ATE399872T1 - Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten - Google Patents

Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten

Info

Publication number
ATE399872T1
ATE399872T1 AT04718972T AT04718972T ATE399872T1 AT E399872 T1 ATE399872 T1 AT E399872T1 AT 04718972 T AT04718972 T AT 04718972T AT 04718972 T AT04718972 T AT 04718972T AT E399872 T1 ATE399872 T1 AT E399872T1
Authority
AT
Austria
Prior art keywords
gene
promoter
expression vectors
vectors containing
mouse cytomegalovirus
Prior art date
Application number
AT04718972T
Other languages
English (en)
Inventor
Philippe Chatellard
Markus Imhof
Original Assignee
Serono Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serono Lab filed Critical Serono Lab
Application granted granted Critical
Publication of ATE399872T1 publication Critical patent/ATE399872T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01DNA viruses
    • C07K14/03Herpetoviridae, e.g. pseudorabies virus
    • C07K14/04Varicella-zoster virus
    • C07K14/045Cytomegalovirus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/16011Herpesviridae
    • C12N2710/16111Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/16011Herpesviridae
    • C12N2710/16111Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16141Use of virus, viral particle or viral elements as a vector
    • C12N2710/16143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/20Vector systems having a special element relevant for transcription transcription of more than one cistron
    • C12N2830/205Vector systems having a special element relevant for transcription transcription of more than one cistron bidirectional
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/60Vector systems having a special element relevant for transcription from viruses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT04718972T 2003-03-11 2004-03-10 Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten ATE399872T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03100617 2003-03-11

Publications (1)

Publication Number Publication Date
ATE399872T1 true ATE399872T1 (de) 2008-07-15

Family

ID=32981915

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04718972T ATE399872T1 (de) 2003-03-11 2004-03-10 Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten

Country Status (26)

Country Link
US (3) US7824907B2 (de)
EP (1) EP1601776B1 (de)
JP (3) JP5171033B2 (de)
KR (1) KR101180139B1 (de)
CN (1) CN100429315C (de)
AT (1) ATE399872T1 (de)
AU (1) AU2004219917B2 (de)
BR (1) BRPI0408246A (de)
CA (1) CA2516157C (de)
CY (1) CY1108344T1 (de)
DE (1) DE602004014738D1 (de)
DK (1) DK1601776T3 (de)
EA (1) EA010096B1 (de)
ES (1) ES2309508T3 (de)
HK (1) HK1085766A1 (de)
HR (1) HRP20050705B1 (de)
IL (1) IL170754A (de)
MX (1) MXPA05009608A (de)
NO (1) NO333818B1 (de)
PL (1) PL1601776T4 (de)
PT (1) PT1601776E (de)
RS (1) RS50488B (de)
SI (1) SI1601776T1 (de)
UA (1) UA88138C2 (de)
WO (1) WO2004081167A2 (de)
ZA (1) ZA200506400B (de)

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US20080076708A1 (en) * 2003-05-13 2008-03-27 Valter Altarocca Active Variants of the Il-18 Binding Protein and Medical Uses Thereof
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ME01218B (me) * 2004-03-01 2013-06-20 Ares Trading Sa Upotreba podloge za ćelijsku strukturu bez sadrzaja seruma za produkciju il-18bp u ćelijama sisara
EP1761552B1 (de) * 2004-06-29 2008-09-17 Ares Trading S.A. Verfahren zur aufreinigung von il-18-bindendem protein
EP1819812B1 (de) * 2004-12-02 2012-05-30 Merck Serono SA Der lupac bifunctionnelle marker und dessen gebrauch in der herstellung von proteinen
EP1874929A2 (de) 2005-04-22 2008-01-09 Lonza Biologics plc Säugerexpressionsvektor mit dem mcmv-promotor und dem ersten intron des major-immediate-early-gens von hcmv
CA2608656A1 (en) * 2005-05-16 2006-11-23 Morphotek, Inc. Regulated vectors for selection of cells exhibiting desired phenotypes
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BRPI0821401A2 (pt) * 2007-12-21 2014-10-14 Novartis Ag Compostos orgânicos
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KR101038126B1 (ko) * 2010-11-30 2011-05-31 주식회사 엘지생명과학 새로운 융합 프로모터 및 이를 포함하는 재조합 벡터
US9249785B2 (en) * 2012-01-31 2016-02-02 Brightsource Industries (Isreal) Ltd. Method and system for operating a solar steam system during reduced-insolation events
EP2711426B1 (de) * 2012-09-24 2015-04-08 Lonza Biologics plc Expressionsvektoren mit chimärem Cytomegalieviruspromotor und Verstärkersequenzen
ITFI20120221A1 (it) * 2012-10-22 2014-04-23 Nuovo Pignone Srl "exhaust gas collector and gas turbine"
WO2014133468A1 (en) * 2013-02-28 2014-09-04 Agency For Science, Technology And Research Chimeric promoters for high gene expression level and stability in mammalian cells
CN103173480B (zh) * 2013-03-01 2015-06-24 上海中医药大学附属曙光医院 一种利用双荧光素酶报告基因筛选多药耐药相关microRNA的方法
CN103173481A (zh) * 2013-03-01 2013-06-26 上海中医药大学附属普陀医院 一种含有abcb1基因3′utr序列和报告基因的质粒载体及其构建方法和用途
EP2970956A1 (de) 2013-03-11 2016-01-20 Novartis AG Verfahren zum screening von zellklonen
ES2689136T3 (es) * 2013-07-31 2018-11-08 Novartis Ag Nuevos vectores de selección y métodos de selección de células hospedadoras eucariotas
PL3083677T3 (pl) 2013-12-20 2020-03-31 Novartis Ag Nowe komórki eukariotyczne i sposoby rekombinowanej ekspresji produktu będącego przedmiotem zainteresowania
KR102288857B1 (ko) 2013-12-20 2021-08-11 노파르티스 아게 신규한 진핵 세포 및 관심 생성물의 재조합 발현 방법
JP6265020B2 (ja) * 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
PT3137595T (pt) 2014-04-29 2019-06-19 Novartis Ag Novas células de vertebrados e métodos para expressão de forma recombinante de um polipéptido de interesse
ES2821940T3 (es) * 2014-05-19 2021-04-28 Hoffmann La Roche Procedimiento para la producción de polipéptidos
KR20160068558A (ko) 2014-12-05 2016-06-15 삼성바이오에피스 주식회사 마우스 cmv 프로모터를 포함하는 융합 폴리뉴클레오타이드 및 이를 이용한 목적 폴리펩타이드의 생산 방법
SG11201706879UA (en) 2015-03-05 2017-09-28 Ab2 Bio Sa Il-18 binding protein (il-18bp) and antibodies in inflammatory diseases
US10570417B2 (en) 2015-04-14 2020-02-25 Janssen Vaccines & Prevention B.V. Recombinant adenovirus expressing two transgenes with a bidirectional promoter
US11028410B2 (en) 2016-01-27 2021-06-08 Just-Evotec Biologics, Inc. Hybrid promoter and uses thereof
RU2758238C2 (ru) * 2016-05-12 2021-10-26 Янссен Вэксинс Энд Превеншн Б.В. Эффективный и сбалансированный двунаправленный промотор
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KR101864581B1 (ko) * 2016-11-04 2018-07-13 국립암센터 양방향 프로모터를 포함하는 렌티바이러스 발현 벡터 및 이의 용도
KR102111244B1 (ko) 2017-02-09 2020-05-15 얀센 백신스 앤드 프리벤션 비.브이. 이종 유전자의 발현을 위한 강력한 짧은 프로모터
JP2020536513A (ja) 2017-10-02 2020-12-17 アストラゼネカ・アクチエボラーグAstrazeneca Aktiebolag タンパク質産生の増加のための細胞株及び方法
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US20080076708A1 (en) 2003-05-13 2008-03-27 Valter Altarocca Active Variants of the Il-18 Binding Protein and Medical Uses Thereof
SI1675956T1 (sl) 2003-10-21 2010-12-31 Merck Serono Sa Minimalno zaporedje dna, ki deluje kot izolirni element kromatina in njegova uporaba pri ekspresiji proteinov
AU2004291341B2 (en) 2003-11-05 2010-08-05 Ares Trading S.A. Process for the purification of IL-18 binding protein
ME01218B (me) 2004-03-01 2013-06-20 Ares Trading Sa Upotreba podloge za ćelijsku strukturu bez sadrzaja seruma za produkciju il-18bp u ćelijama sisara
EP1761552B1 (de) 2004-06-29 2008-09-17 Ares Trading S.A. Verfahren zur aufreinigung von il-18-bindendem protein
EP1819812B1 (de) * 2004-12-02 2012-05-30 Merck Serono SA Der lupac bifunctionnelle marker und dessen gebrauch in der herstellung von proteinen
PT1885753E (pt) 2005-06-03 2011-10-06 Ares Trading Sa Produção de proteína recombinante de ligação a il-18
CA2610804C (en) 2005-06-10 2013-11-19 Ares Trading S.A. Process for the purification of il-18 binding protein
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Also Published As

Publication number Publication date
US20150252383A1 (en) 2015-09-10
KR101180139B1 (ko) 2012-09-13
US20070258962A1 (en) 2007-11-08
MXPA05009608A (es) 2006-03-21
SI1601776T1 (sl) 2008-10-31
AU2004219917B2 (en) 2008-01-31
HRP20050705A2 (en) 2005-12-31
JP5848202B2 (ja) 2016-01-27
JP5171033B2 (ja) 2013-03-27
KR20050113193A (ko) 2005-12-01
EP1601776A2 (de) 2005-12-07
NO20054642L (no) 2005-10-10
US7824907B2 (en) 2010-11-02
CN1759184A (zh) 2006-04-12
ZA200506400B (en) 2006-12-27
UA88138C2 (en) 2009-09-25
BRPI0408246A (pt) 2006-03-01
RS20050687A (en) 2007-11-15
CA2516157C (en) 2012-09-18
HK1085766A1 (en) 2006-09-01
PT1601776E (pt) 2008-09-17
NO333818B1 (no) 2013-09-23
RS50488B (sr) 2010-03-02
JP2012235787A (ja) 2012-12-06
EA010096B1 (ru) 2008-06-30
AU2004219917A1 (en) 2004-09-23
EP1601776B1 (de) 2008-07-02
DK1601776T3 (da) 2008-10-13
HRP20050705B1 (hr) 2013-08-31
PL1601776T4 (pl) 2009-05-29
CN100429315C (zh) 2008-10-29
EA200501444A1 (ru) 2006-02-24
US9051582B2 (en) 2015-06-09
US20110008839A1 (en) 2011-01-13
WO2004081167A3 (en) 2004-11-04
PL1601776T3 (pl) 2008-12-31
ES2309508T3 (es) 2008-12-16
CY1108344T1 (el) 2014-02-12
CA2516157A1 (en) 2004-09-23
JP2006520589A (ja) 2006-09-14
WO2004081167A2 (en) 2004-09-23
JP2015180222A (ja) 2015-10-15
DE602004014738D1 (de) 2008-08-14
IL170754A (en) 2010-11-30

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