DE60330140D1 - Gen-silencing-vektor und diesen verwendendes gen-silencing-verfahren - Google Patents

Gen-silencing-vektor und diesen verwendendes gen-silencing-verfahren

Info

Publication number
DE60330140D1
DE60330140D1 DE60330140T DE60330140T DE60330140D1 DE 60330140 D1 DE60330140 D1 DE 60330140D1 DE 60330140 T DE60330140 T DE 60330140T DE 60330140 T DE60330140 T DE 60330140T DE 60330140 D1 DE60330140 D1 DE 60330140D1
Authority
DE
Germany
Prior art keywords
silencing
gene
vector
gen
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60330140T
Other languages
English (en)
Inventor
Keisuke Kasaoka
Yasuhito Saito
Shigeru Kuwata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Application granted granted Critical
Publication of DE60330140D1 publication Critical patent/DE60330140D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compositions Of Oxide Ceramics (AREA)
  • Inorganic Insulating Materials (AREA)
  • Saccharide Compounds (AREA)
DE60330140T 2002-05-22 2003-05-21 Gen-silencing-vektor und diesen verwendendes gen-silencing-verfahren Expired - Lifetime DE60330140D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002147888 2002-05-22
PCT/JP2003/006328 WO2003097841A1 (fr) 2002-05-22 2003-05-21 Vecteur de silençage genique et procede de silençage genique utilisant ce vecteur

Publications (1)

Publication Number Publication Date
DE60330140D1 true DE60330140D1 (de) 2009-12-31

Family

ID=29545204

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60330140T Expired - Lifetime DE60330140D1 (de) 2002-05-22 2003-05-21 Gen-silencing-vektor und diesen verwendendes gen-silencing-verfahren

Country Status (9)

Country Link
EP (1) EP1507009B1 (de)
JP (1) JP4361858B2 (de)
AT (1) ATE449182T1 (de)
AU (1) AU2003235377A1 (de)
DE (1) DE60330140D1 (de)
DK (1) DK1507009T3 (de)
ES (1) ES2335756T3 (de)
PT (1) PT1507009E (de)
WO (1) WO2003097841A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110857438B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用
CN110857439B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的马铃薯Y病毒基因片段、弱毒疫苗、制备方法及其应用
CN110885797B (zh) * 2018-08-20 2022-04-12 山东农业大学 一种抗黄瓜花叶病毒的弱毒疫苗、制备方法及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3452372B2 (ja) * 1992-10-21 2003-09-29 日本たばこ産業株式会社 組換えベクター及びそれを用いてジャガイモにｐｖｙ−ｔに対する免疫性を付与する方法並びにｐｖｙ−ｔ免疫性ジャガイモ
GB9703146D0 (en) * 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants
AU2002347343A1 (en) * 2001-12-12 2003-06-23 Plant Bioscience Limited Methods and means for gene silencing in plants

Also Published As

Publication number Publication date
PT1507009E (pt) 2010-01-25
EP1507009A4 (de) 2006-03-22
EP1507009A1 (de) 2005-02-16
DK1507009T3 (da) 2010-04-06
ATE449182T1 (de) 2009-12-15
JPWO2003097841A1 (ja) 2005-09-15
JP4361858B2 (ja) 2009-11-11
AU2003235377A1 (en) 2003-12-02
EP1507009B1 (de) 2009-11-18
ES2335756T3 (es) 2010-04-05
WO2003097841A1 (fr) 2003-11-27

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