UA92913C2 - Экспрессионный вектор и способы продуцирования высоких уровней белков - Google Patents

Экспрессионный вектор и способы продуцирования высоких уровней белков

Info

Publication number
UA92913C2
UA92913C2 UAA200713742A UAA200713742A UA92913C2 UA 92913 C2 UA92913 C2 UA 92913C2 UA A200713742 A UAA200713742 A UA A200713742A UA A200713742 A UAA200713742 A UA A200713742A UA 92913 C2 UA92913 C2 UA 92913C2
Authority
UA
Ukraine
Prior art keywords
expression vector
proteins
production
methods
high levels
Prior art date
Application number
UAA200713742A
Other languages
English (en)
Ukrainian (uk)
Inventor
Арун К. Сингх
Ашиш Гоел
Сенджив К. Мендиратта
Original Assignee
Кадила Хелткере Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Кадила Хелткере Лимитед filed Critical Кадила Хелткере Лимитед
Publication of UA92913C2 publication Critical patent/UA92913C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/60—Vectors comprising a special translation-regulating system from viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Способ получения высокой экспрессии нужного белка с помощью экспрессионного вектора. Способ предусматривает использование экспрессионного вектора, который состоит из как минимум одного из следующих регуляторных элементов: а) промотора CMV или его функциональных вариантов, b) интрона, с) TPL или его функциональных вариантов, d) генов VA или функциональных вариантов, е) полиадениляционной последовательности бычьего гормона роста или функциональных вариантов.
UAA200713742A 2005-06-20 2006-06-19 Экспрессионный вектор и способы продуцирования высоких уровней белков UA92913C2 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN720MU2005 2005-06-20

Publications (1)

Publication Number Publication Date
UA92913C2 true UA92913C2 (ru) 2010-12-27

Family

ID=37727723

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200713742A UA92913C2 (ru) 2005-06-20 2006-06-19 Экспрессионный вектор и способы продуцирования высоких уровней белков

Country Status (13)

Country Link
US (2) US9163257B2 (ru)
EP (1) EP1893757B1 (ru)
JP (1) JP5073653B2 (ru)
KR (1) KR101184074B1 (ru)
AU (1) AU2006277568B2 (ru)
CA (1) CA2611946C (ru)
EA (1) EA011619B1 (ru)
ES (1) ES2605358T3 (ru)
HK (1) HK1112020A1 (ru)
NZ (1) NZ592891A (ru)
UA (1) UA92913C2 (ru)
WO (1) WO2007017903A2 (ru)
ZA (1) ZA200710554B (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101607734B1 (ko) 2010-10-08 2016-03-30 카딜라 핼쓰캐어 리미티드 재조합 단백질의 높은 레벨의 발현을 위한 발현 벡터
KR101599138B1 (ko) 2014-06-17 2016-03-03 한국생명공학연구원 재조합 단백질 발현 증진을 위한 유전자 절편을 포함하는 벡터 및 이의 용도
WO2017027324A1 (en) * 2015-08-10 2017-02-16 The Texas A&M University System Recombinant turkey herpesvirus vaccines and uses thereof
AU2018327224A1 (en) 2017-09-07 2020-04-23 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptide with conjugation sites and methods of use thereof
US12071633B2 (en) * 2020-10-13 2024-08-27 Kriya Therapeutics, Inc. Viral vector constructs for delivery of nucleic acids encoding cytokines and uses thereof for treating cancer
US20230414777A1 (en) * 2020-11-06 2023-12-28 Cue Biopharma, Inc. Antigen Presenting Polypeptide Complexes and Methods of Use Thereof
CN114574444B (zh) * 2020-12-01 2024-05-03 达达生物技术(北京)有限公司 自体纤维母细胞在制备抗类风湿关节炎药物中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
US6040295A (en) * 1995-01-13 2000-03-21 Genemedicine, Inc. Formulated nucleic acid compositions and methods of administering the same for gene therapy
DE19539493A1 (de) * 1995-10-24 1997-04-30 Thomae Gmbh Dr K Starker homologer Promotor aus Hamster
ES2243995T3 (es) * 1996-04-26 2005-12-01 Beth Israel Deaconess Medical Center, Inc. Antagonistas de interleucina-15.
CA2269654A1 (en) * 1996-08-23 1998-02-26 Arch Development Corporation Long-term expression of gene products by transforming muscle cells
GB9817660D0 (en) * 1998-08-13 1998-10-07 Angeletti P Ist Richerche Bio Adenoviral vectors encoding erythropoietin and their use in gene therapy
US6777205B1 (en) * 1998-11-06 2004-08-17 Sterrenbeld Biotechnologie North America, Inc. Host cells expressing recombinant human erythropoietin
BR9905867A (pt) * 1998-11-06 2001-01-23 Bio Sidus S A Linhagem celular produtora de eritropoietina humana recombinante e a eritropoietina humana recombinante produzida por esta célula
US7294481B1 (en) * 1999-01-05 2007-11-13 Immunex Corporation Method for producing recombinant proteins
AU2001257331A1 (en) * 2000-04-28 2001-11-12 Sangamo Biosciences, Inc. Methods for designing exogenous regulatory molecules
JP2003055266A (ja) * 2001-06-04 2003-02-26 Univ Texas Syst Ｍｅｋ５ならびに心臓肥大および拡張型心筋症に関連する方法および組成物
US20030157641A1 (en) * 2001-11-16 2003-08-21 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
KR100533794B1 (ko) * 2003-10-02 2005-12-07 주식회사 프로젠 인간 난포자극 호르몬을 대량으로 생산하는 방법

Also Published As

Publication number Publication date
KR20080047319A (ko) 2008-05-28
ZA200710554B (en) 2008-10-29
EP1893757B1 (en) 2016-09-07
US9163257B2 (en) 2015-10-20
US20130236957A1 (en) 2013-09-12
EP1893757A2 (en) 2008-03-05
AU2006277568A2 (en) 2008-07-17
ES2605358T3 (es) 2017-03-14
US20110212518A1 (en) 2011-09-01
CA2611946C (en) 2013-04-16
JP5073653B2 (ja) 2012-11-14
HK1112020A1 (zh) 2008-08-22
JP2008543325A (ja) 2008-12-04
AU2006277568A1 (en) 2007-02-15
EA200800098A1 (ru) 2008-06-30
WO2007017903A2 (en) 2007-02-15
US9260721B2 (en) 2016-02-16
KR101184074B1 (ko) 2012-09-17
CA2611946A1 (en) 2007-02-15
EA011619B1 (ru) 2009-04-28
AU2006277568B2 (en) 2011-06-30
WO2007017903A3 (en) 2007-06-28
NZ592891A (en) 2013-02-22

Similar Documents

Publication Publication Date Title
UA92913C2 (ru) Экспрессионный вектор и способы продуцирования высоких уровней белков
WO2019126762A3 (en) Cas12a systems, methods, and compositions for targeted rna base editing
WO2007003582A3 (en) Serum-free stable transfection and production of recombinant human proteins in human cell lines
FI3536797T3 (fi) Siirtogeenin tehostettu ilmentäminen ja prosessointi
PH12020551039A1 (en) Non-viral dna vectors and uses thereof for antibody and fusion protein production
ZA200705777B (en) The transcription factor gene osnacx from rice and use for improving tolerance of plants to drought and salt thereof
WO2005102034A8 (en) Regulatory sequences for expressing gene products in plant reproductive tissue
EP1801206A4 (en) PROCESS FOR THE PREPARATION OF L-ARGININE, L-ORNITHIN OR L-CITRULLINE
HK1136847A1 (en) Targeted integration into the ppp1r12c locus
MX2010000196A (es) Prevencion de reduccion de enlaces de disulfuro durante la produccion recombinante de polipeptidos.
MX2010004807A (es) Evolucion dirigida utilizando proteinas que comprenden aminoacidos no naturales.
WO2006056885A3 (en) Igf-1 isoforms
IL172729A0 (en) Reagents and methods for the formation of disulfide bonds and the glycosylation of proteins
WO2007051063A3 (en) Gpcr expressing cell lines and antibodies
TW200638944A (en) Recombinant enterovirus 71 neutralizing antibodies and use thereof
WO2006111387A3 (en) Mammalian expression vector comprising the mcmv promoter and first intron of hcmv major immediate early gene
WO2010002160A3 (ko) 돼지의 알파에스 1 카제인 유전자, 그 프로모터 및 그의 용도
WO2021087296A8 (en) Compositions and methods for treating sensorineural hearing loss using otoferlin dual vector systems
WO2007015691A3 (en) Methods and compositions for increasing longevity and protein yield from a cell culture
ATE532861T1 (de) Expressionsvektor
EP1870454A4 (en) PROCESS FOR DIPEPTIDE PRODUCTION
WO2007088051A3 (en) Modulation of mdl-1 activity for treatment of inflammatory disease
MY147959A (en) Productivity augmenting protein factor, novel cell lines and uses thereof
UA86781C2 (ru) Промотор для эпидермис-специфической трансгенной экспрессии в растениях
ATE442441T1 (de) Virale expressionsvektoren für pflanzen