ES2546086T3 - Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana - Google Patents

Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Download PDF

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Publication number
ES2546086T3
ES2546086T3 ES08865304.3T ES08865304T ES2546086T3 ES 2546086 T3 ES2546086 T3 ES 2546086T3 ES 08865304 T ES08865304 T ES 08865304T ES 2546086 T3 ES2546086 T3 ES 2546086T3
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Prior art keywords
membrane
cell culture
eukaryotic cell
selection system
receptor gene
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ES08865304.3T
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English (en)
Inventor
Yehuda G. Assaraf
Thomas Jostock
Hans-Peter Knopf
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Novartis AG
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un vector de expresión en eucariotas que comprende un primer polinucleótido que codifica un receptor funcional de folato unido a la membrana y un segundo polinucleótido que codifica un producto de interés, en donde el producto de interés es un polipéptido farmacéutica o terapéuticamente activo o de diagnóstico.

Description

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Claims (1)

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ES08865304.3T 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Active ES2546086T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP07150326 2007-12-21
EP07150326 2007-12-21
PCT/EP2008/068046 WO2009080759A1 (en) 2007-12-21 2008-12-19 Organic compounds

Publications (1)

Publication Number Publication Date
ES2546086T3 true ES2546086T3 (es) 2015-09-18

Family

ID=39560901

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08865304.3T Active ES2546086T3 (es) 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana

Country Status (15)

Country Link
US (5) US20100330572A1 (es)
EP (1) EP2227549B1 (es)
JP (2) JP5660900B2 (es)
KR (1) KR101706390B1 (es)
CN (2) CN101903526B (es)
AU (1) AU2008340017B2 (es)
BR (1) BRPI0821401A2 (es)
CA (1) CA2709503C (es)
DK (1) DK2227549T3 (es)
ES (1) ES2546086T3 (es)
IL (1) IL206233A (es)
PL (1) PL2227549T3 (es)
PT (1) PT2227549E (es)
RU (1) RU2502802C2 (es)
WO (1) WO2009080759A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG173456A1 (en) 2009-02-27 2011-09-29 Novartis Ag Expression vector system comprising two selection markers
UA123257C2 (uk) 2010-02-24 2021-03-10 Іммуноджен, Інк. Виділений поліпептид, що кодує антитіло до рецептора фолієвої кислоти 1
PT2694106T (pt) 2011-04-01 2018-03-05 Immunogen Inc Métodos para aumentar eficácia de terapia de cancro de folr1
NZ726258A (en) 2012-08-31 2019-07-26 Immunogen Inc Antibodies and uses thereof to detect folate receptor 1
LT2922962T (lt) 2012-11-20 2017-04-10 Novartis Ag Optimizuota raiškos kasetė, skirta polipeptido raiškai su didele išeiga
EP2970956A1 (en) 2013-03-11 2016-01-20 Novartis AG Method of screening cell clones
EP3412684B1 (en) 2013-07-31 2022-04-06 Novartis AG Novel selection vectors and methods of selecting eukaryotic host cells
SG10201907501QA (en) 2013-08-30 2019-10-30 Immunogen Inc Antibodies and assays for detection of folate receptor 1
CN106029692A (zh) 2013-12-20 2016-10-12 诺华股份有限公司 用于重组表达感兴趣产物的新型真核细胞和方法
RU2712507C2 (ru) 2013-12-20 2020-01-29 Новартис Аг Новые эукариотические клетки и способы их получения для рекомбинантной экспрессии представляющего интерес продукта
PL3536776T3 (pl) 2014-04-29 2024-03-25 Novartis Ag Nowe komórki kręgowców i sposoby ekspresji rekombinowanej polipeptydu będącego przedmiotem zainteresowania
PL3277818T3 (pl) * 2015-04-03 2020-06-15 Selexis S.A. Zastosowanie witamin oraz genów i białek metabolizmu witamin do wytwarzania białek rekombinowanych w komórkach ssaczych
JP6768786B2 (ja) * 2015-07-30 2020-10-14 エクスプレッション、パソロジー、インコーポレイテッドＥｘｐｒｅｓｓｉｏｎ Ｐａｔｈｏｌｏｇｙ， Ｉｎｃ． 最適な癌療法のためのＦＲ−αおよびＧＡＲＴタンパク質の定量
KR102700777B1 (ko) 2015-09-17 2024-08-29 이뮤노젠 아이엔씨 항-folr1 면역접합체를 포함하는 치료제 조합
US20200370056A1 (en) 2017-10-02 2020-11-26 Astrazeneca Ab Cell lines and methods for increased protein production
WO2022107090A1 (en) 2020-11-23 2022-05-27 Novartis Ag Expression technology for antibody constructs
EP4330383A1 (en) 2021-04-29 2024-03-06 Novartis AG Hypersialylating cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0555203B1 (en) * 1988-12-08 1995-10-18 Abbott Laboratories Expression induction method employing dhfr gene
IL162476A (en) 1995-02-15 2005-12-18 Aventis Pharma Sa Arabidopsis thaliana proteins having delta -5,7- sterol - delta -7- reductase activity
JP5171033B2 (ja) * 2003-03-11 2013-03-27 メルク セローノ ソシエテ アノニム ｍＣＭＶＩＥ２プロモーターを含んで成る発現ベクター
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
CN100567482C (zh) * 2006-10-12 2009-12-09 南开大学 高效表达rhBMP2的CHO细胞株及其建立方法

Also Published As

Publication number Publication date
RU2010129550A (ru) 2012-01-27
KR101706390B1 (ko) 2017-02-13
DK2227549T3 (en) 2015-09-21
PL2227549T3 (pl) 2015-12-31
RU2502802C2 (ru) 2013-12-27
IL206233A (en) 2015-10-29
JP2014236745A (ja) 2014-12-18
US20100330572A1 (en) 2010-12-30
JP6002183B2 (ja) 2016-10-05
AU2008340017B2 (en) 2012-04-05
CN101903526A (zh) 2010-12-01
CN101903526B (zh) 2014-02-12
CN103805633A (zh) 2014-05-21
CN103805633B (zh) 2016-09-07
EP2227549A1 (en) 2010-09-15
CA2709503A1 (en) 2009-07-02
US20120276579A1 (en) 2012-11-01
US20140295489A1 (en) 2014-10-02
JP5660900B2 (ja) 2015-01-28
WO2009080759A1 (en) 2009-07-02
EP2227549B1 (en) 2015-06-17
US20200370055A1 (en) 2020-11-26
BRPI0821401A2 (pt) 2014-10-14
US20160355828A1 (en) 2016-12-08
KR20100111698A (ko) 2010-10-15
IL206233A0 (en) 2010-12-30
AU2008340017A1 (en) 2009-07-02
US10767186B2 (en) 2020-09-08
JP2011505861A (ja) 2011-03-03
PT2227549E (pt) 2015-10-08
CA2709503C (en) 2016-07-12

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