ES2546086T3 - Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana - Google Patents

Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Download PDF

Info

Publication number
ES2546086T3
ES2546086T3 ES08865304.3T ES08865304T ES2546086T3 ES 2546086 T3 ES2546086 T3 ES 2546086T3 ES 08865304 T ES08865304 T ES 08865304T ES 2546086 T3 ES2546086 T3 ES 2546086T3
Authority
ES
Spain
Prior art keywords
membrane
cell culture
eukaryotic cell
selection system
receptor gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08865304.3T
Other languages
English (en)
Inventor
Yehuda G. Assaraf
Thomas Jostock
Hans-Peter Knopf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Application granted granted Critical
Publication of ES2546086T3 publication Critical patent/ES2546086T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Abstract

Un vector de expresión en eucariotas que comprende un primer polinucleótido que codifica un receptor funcional de folato unido a la membrana y un segundo polinucleótido que codifica un producto de interés, en donde el producto de interés es un polipéptido farmacéutica o terapéuticamente activo o de diagnóstico.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
imagen10

Claims (1)

  1. imagen1
    imagen2
ES08865304.3T 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Active ES2546086T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP07150326 2007-12-21
EP07150326 2007-12-21
PCT/EP2008/068046 WO2009080759A1 (en) 2007-12-21 2008-12-19 Organic compounds

Publications (1)

Publication Number Publication Date
ES2546086T3 true ES2546086T3 (es) 2015-09-18

Family

ID=39560901

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08865304.3T Active ES2546086T3 (es) 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana

Country Status (15)

Country Link
US (5) US20100330572A1 (es)
EP (1) EP2227549B1 (es)
JP (2) JP5660900B2 (es)
KR (1) KR101706390B1 (es)
CN (2) CN103805633B (es)
AU (1) AU2008340017B2 (es)
BR (1) BRPI0821401A2 (es)
CA (1) CA2709503C (es)
DK (1) DK2227549T3 (es)
ES (1) ES2546086T3 (es)
IL (1) IL206233A (es)
PL (1) PL2227549T3 (es)
PT (1) PT2227549E (es)
RU (1) RU2502802C2 (es)
WO (1) WO2009080759A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010097240A1 (en) 2009-02-27 2010-09-02 Novartis Ag Expression vector system comprising two selection markers
NZ724971A (en) 2010-02-24 2019-06-28 Immunogen Inc Folate receptor 1 antibodies and immunoconjugates and uses thereof
MX340640B (es) 2011-04-01 2016-07-19 Immunogen Inc Metodos para aumentar la eficacia de la terapia contra el cancer con receptor 1 de folato (folr1).
KR20240005129A (ko) 2012-08-31 2024-01-11 이뮤노젠 아이엔씨 엽산 수용체 1의 검출을 위한 진단성 검정 및 키트
JP6388592B2 (ja) 2012-11-20 2018-09-12 ノバルティス アーゲー ポリペプチドを高収量で発現するための最適化発現カセット
WO2014141037A1 (en) 2013-03-11 2014-09-18 Novartis Ag Method of screening cell clones
LT3027646T (lt) 2013-07-31 2018-09-25 Novartis Ag Nauji atrankos vektoriai ir eukariotinių ląstelių šeimininkių atrankos būdai
IL293871A (en) 2013-08-30 2022-08-01 Immunogen Inc Antibodies and assays for the detection of folate receptor 1
DK3083676T3 (da) 2013-12-20 2019-11-25 Novartis Ag Hidtil ukendte eukaryote celler og fremgangsmåder til rekombinant ekspression af et produkt af interesse
LT3083677T (lt) 2013-12-20 2019-12-10 Novartis Ag Naujos eukariotinės ląstelės ir dominančio polipeptido rekombinantinės raiškos būdai
PT3137595T (pt) 2014-04-29 2019-06-19 Novartis Ag Novas células de vertebrados e métodos para expressão de forma recombinante de um polipéptido de interesse
SG11201706617SA (en) * 2015-04-03 2017-09-28 Selexis Sa Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells
US9746477B2 (en) * 2015-07-30 2017-08-29 Expression Pathology, Inc. Quantifying FR-α and GART proteins for optimal cancer therapy
JP6880006B2 (ja) 2015-09-17 2021-06-02 イミュノジェン， インコーポレイテッド 抗ｆｏｌｒ１免疫複合体を含む治療組み合わせ
US20200370056A1 (en) 2017-10-02 2020-11-26 Astrazeneca Ab Cell lines and methods for increased protein production
CN116615462A (zh) 2020-11-23 2023-08-18 诺华股份有限公司 抗体构建体的表达技术
EP4330383A1 (en) 2021-04-29 2024-03-06 Novartis AG Hypersialylating cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE129289T1 (de) * 1988-12-08 1995-11-15 Abbott Lab Verfahren zur induzierbaren genexpression unter verwendung eines dhfr-gens.
IL116872A (en) 1995-02-15 2004-12-15 Aventis Pharma Sa Delta 5,7-Strol Reductase from Arbidopsis Thaliana
WO2004081167A2 (en) * 2003-03-11 2004-09-23 Applied Research Systems Ars Holding N.V. Expression vectors comprising the mcmv ie2 promoter
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
CN100567482C (zh) * 2006-10-12 2009-12-09 南开大学 高效表达rhBMP2的CHO细胞株及其建立方法

Also Published As

Publication number Publication date
EP2227549B1 (en) 2015-06-17
US20140295489A1 (en) 2014-10-02
PL2227549T3 (pl) 2015-12-31
CN103805633A (zh) 2014-05-21
PT2227549E (pt) 2015-10-08
KR101706390B1 (ko) 2017-02-13
CA2709503C (en) 2016-07-12
CN103805633B (zh) 2016-09-07
WO2009080759A1 (en) 2009-07-02
CA2709503A1 (en) 2009-07-02
JP2011505861A (ja) 2011-03-03
AU2008340017B2 (en) 2012-04-05
JP6002183B2 (ja) 2016-10-05
US20200370055A1 (en) 2020-11-26
RU2010129550A (ru) 2012-01-27
JP2014236745A (ja) 2014-12-18
DK2227549T3 (en) 2015-09-21
BRPI0821401A2 (pt) 2014-10-14
US20120276579A1 (en) 2012-11-01
IL206233A0 (en) 2010-12-30
KR20100111698A (ko) 2010-10-15
CN101903526A (zh) 2010-12-01
US20100330572A1 (en) 2010-12-30
IL206233A (en) 2015-10-29
EP2227549A1 (en) 2010-09-15
US20160355828A1 (en) 2016-12-08
US10767186B2 (en) 2020-09-08
JP5660900B2 (ja) 2015-01-28
AU2008340017A1 (en) 2009-07-02
RU2502802C2 (ru) 2013-12-27
CN101903526B (zh) 2014-02-12

Similar Documents

Publication Publication Date Title
ES2546086T3 (es) Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana
ES2486675T3 (es) Análogo de péptido de oxintomodulina
ES2484417T3 (es) Perfil de expresión génica como marcador de la receptividad endometrial
ES2616658T3 (es) Péptidos que mimetizan el factor de crecimiento y sus usos
WO2009041586A1 (ja) 診断支援装置及びその制御方法
MX2007007033A (es) Estreptococus del grupo b.
WO2007117535A3 (en) Parcel imaging system and method
BRPI0600115A (pt) sistemas e métodos para regular corrente de pré-carga em um sistema de bateria
EP4279136A3 (en) Methods for determining car-t cells dosing
ES2720999T3 (es) Método y sistema para seleccionar un fármaco sobre la base de la información del daño de proteína individual para prevenir los efectos secundarios de un fármaco
ES2527454T3 (es) El uso de derivados del péptido Wnt5-a para el tratamiento de melanoma y cáncer gástrico
Ben-Shalom Hasdai Crescas: Portrait of a leader at a time of crisis
BR112012031234A2 (pt) "sistema e métodos de sensor de posição modular"
Vinevskaya On the issue of information component of the mobile educational environment
Guo et al. Binding of the vesicle docking protein p115 to the GTPase Rab1b regulates membrane recruitment of the COPI vesicle coat
ES2654208T3 (es) Celda electroquímica y reactor que comprende la misma
Baldina et al. Interpretation of multi-temporal space imagery in thermal infrared band
GB2565604A8 (en) Portable radar sensing device
D'Agostino Lessons learned from imaging on rheumatoid arthritis
Mein-Woei Photograph Stimuli are More Appropriate Stimuli to Emerge Related Stereotypes
Obidua SCHOOL OF POSTGRADUATE STUDIES
Shal’nev et al. General Geography: myth or reality
Ziller et al. Back Cover: Poly (benzoxazine) as an Immobilization Matrix for Miniaturized ATP and Glucose Biosensors (ChemElectroChem 4/2017).
Tsurimoto et al. Report from the Working Group of the Molecular Biology Society of Japan for the investigation of fraud in research papers
Marchii-Dmytrash Criteria of foreign languages future teachers’ readiness to the organization of educational activities in elementary school