PH25990A - Site selective genomic modification of yeast of the genus pichia - Google Patents
Site selective genomic modification of yeast of the genus pichia Download PDFInfo
- Publication number
- PH25990A PH25990A PH34343A PH34343A PH25990A PH 25990 A PH25990 A PH 25990A PH 34343 A PH34343 A PH 34343A PH 34343 A PH34343 A PH 34343A PH 25990 A PH25990 A PH 25990A
- Authority
- PH
- Philippines
- Prior art keywords
- gene
- dna
- dna fragment
- insertable
- fragments
- Prior art date
Links
- 238000010362 genome editing Methods 0.000 title claims abstract description 17
- 241000235648 Pichia Species 0.000 title abstract description 58
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract description 29
- 108020004414 DNA Proteins 0.000 claims abstract description 137
- 239000012634 fragment Substances 0.000 claims abstract description 127
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 109
- 108010025188 Alcohol oxidase Proteins 0.000 claims abstract description 41
- 239000003550 marker Substances 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 27
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 19
- 239000013612 plasmid Substances 0.000 claims description 60
- 241000235058 Komagataella pastoris Species 0.000 claims description 26
- 230000001131 transforming effect Effects 0.000 claims description 16
- 230000001580 bacterial effect Effects 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 10
- 125000003729 nucleotide group Chemical group 0.000 claims description 10
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 101150061183 AOX1 gene Proteins 0.000 claims description 8
- 101150006240 AOX2 gene Proteins 0.000 claims description 6
- ZQISRDCJNBUVMM-UHFFFAOYSA-N L-Histidinol Natural products OCC(N)CC1=CN=CN1 ZQISRDCJNBUVMM-UHFFFAOYSA-N 0.000 claims description 2
- ZQISRDCJNBUVMM-YFKPBYRVSA-N L-histidinol Chemical compound OC[C@@H](N)CC1=CNC=N1 ZQISRDCJNBUVMM-YFKPBYRVSA-N 0.000 claims description 2
- 108010085336 phosphoribosyl-AMP cyclohydrolase Proteins 0.000 claims 3
- 108010020056 Hydrogenase Proteins 0.000 claims 1
- 230000014509 gene expression Effects 0.000 abstract description 19
- 238000004519 manufacturing process Methods 0.000 abstract description 14
- 150000003138 primary alcohols Chemical class 0.000 abstract description 8
- 230000006798 recombination Effects 0.000 abstract description 2
- 238000005215 recombination Methods 0.000 abstract description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 81
- 210000004027 cell Anatomy 0.000 description 42
- 229920001817 Agar Polymers 0.000 description 38
- 239000008272 agar Substances 0.000 description 38
- 239000002609 medium Substances 0.000 description 21
- 239000013598 vector Substances 0.000 description 20
- 230000012010 growth Effects 0.000 description 18
- 230000009466 transformation Effects 0.000 description 18
- 230000008929 regeneration Effects 0.000 description 17
- 238000011069 regeneration method Methods 0.000 description 17
- 241000588724 Escherichia coli Species 0.000 description 15
- 235000014304 histidine Nutrition 0.000 description 15
- 238000003780 insertion Methods 0.000 description 15
- 230000037431 insertion Effects 0.000 description 15
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 14
- 229960002885 histidine Drugs 0.000 description 14
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 14
- 239000000047 product Substances 0.000 description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 13
- 102000004196 processed proteins & peptides Human genes 0.000 description 13
- 108090000765 processed proteins & peptides Proteins 0.000 description 13
- 230000000694 effects Effects 0.000 description 12
- 239000000600 sorbitol Substances 0.000 description 12
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000012217 deletion Methods 0.000 description 9
- 230000037430 deletion Effects 0.000 description 9
- 108010067193 Formaldehyde transketolase Proteins 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 229920001184 polypeptide Polymers 0.000 description 8
- 239000000872 buffer Substances 0.000 description 7
- 230000002950 deficient Effects 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 238000010276 construction Methods 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 241000364057 Peoria Species 0.000 description 5
- 241000235070 Saccharomyces Species 0.000 description 5
- 238000002105 Southern blotting Methods 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 239000008121 dextrose Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 4
- KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 102000002464 Galactosidases Human genes 0.000 description 4
- 108010093031 Galactosidases Proteins 0.000 description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 238000000844 transformation Methods 0.000 description 4
- 239000007222 ypd medium Substances 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108090000854 Oxidoreductases Proteins 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 101150031623 aox gene Proteins 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 210000005253 yeast cell Anatomy 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 2
- 101000583086 Bunodosoma granuliferum Delta-actitoxin-Bgr2b Proteins 0.000 description 2
- 108020004638 Circular DNA Proteins 0.000 description 2
- 101150069554 HIS4 gene Proteins 0.000 description 2
- 108090000856 Lyases Proteins 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 2
- 244000292604 Salvia columbariae Species 0.000 description 2
- 235000012377 Salvia columbariae var. columbariae Nutrition 0.000 description 2
- 235000001498 Salvia hispanica Nutrition 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- YSVZGWAJIHWNQK-UHFFFAOYSA-N [3-(hydroxymethyl)-2-bicyclo[2.2.1]heptanyl]methanol Chemical compound C1CC2C(CO)C(CO)C1C2 YSVZGWAJIHWNQK-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000014167 chia Nutrition 0.000 description 2
- 230000035611 feeding Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- -1 for example Proteins 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 101150066555 lacZ gene Proteins 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000012808 vapor phase Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 101150033839 4 gene Proteins 0.000 description 1
- 101150041132 AO gene Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000040944 Carpobrotus aequilaterus Species 0.000 description 1
- 235000005809 Carpobrotus aequilaterus Nutrition 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 101150105088 Dele1 gene Proteins 0.000 description 1
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 description 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 241001508691 Martes zibellina Species 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 208000003629 Rupture Diseases 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 101100323865 Xenopus laevis arg1 gene Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 239000012143 dye reagent concentrate Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000001279 glycosylating effect Effects 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 150000002411 histidines Chemical class 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 125000006501 nitrophenyl group Chemical group 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001698 pyrogenic effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000036259 susceptibility to 4 autoimmune disease Diseases 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/791,013 US4882279A (en) | 1985-10-25 | 1985-10-25 | Site selective genomic modification of yeast of the genus pichia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PH25990A true PH25990A (en) | 1992-01-13 |
Family
ID=25152397
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PH34343A PH25990A (en) | 1985-10-25 | 1986-10-09 | Site selective genomic modification of yeast of the genus pichia |
Country Status (26)
| Country | Link |
|---|---|
| US (1) | US4882279A (sh) |
| EP (2) | EP0226752B1 (sh) |
| JP (1) | JP2614215B2 (sh) |
| KR (1) | KR930002738B1 (sh) |
| CN (1) | CN86107108A (sh) |
| AT (2) | ATE197962T1 (sh) |
| AU (2) | AU581107B2 (sh) |
| CA (1) | CA1339209C (sh) |
| DD (1) | DD255544A5 (sh) |
| DE (2) | DE3650749T2 (sh) |
| DK (1) | DK510786A (sh) |
| EG (1) | EG17985A (sh) |
| ES (2) | ES2061433T3 (sh) |
| FI (1) | FI98931C (sh) |
| HU (1) | HU208552B (sh) |
| IE (1) | IE68454B1 (sh) |
| IL (1) | IL80286A (sh) |
| IN (1) | IN166443B (sh) |
| MX (1) | MX168390B (sh) |
| NO (1) | NO176923C (sh) |
| NZ (1) | NZ217809A (sh) |
| PH (1) | PH25990A (sh) |
| PL (1) | PL154843B1 (sh) |
| PT (1) | PT83618B (sh) |
| YU (1) | YU46758B (sh) |
| ZA (1) | ZA867650B (sh) |
Families Citing this family (162)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5032516A (en) * | 1985-10-25 | 1991-07-16 | Phillips Petroleum Company | Pichia pastoris alcohol oxidase II regulatory region |
| US4895800A (en) * | 1985-11-26 | 1990-01-23 | Phillips Petroleum Company | Yeast production of hepatitis B surface antigen |
| IL89993A0 (en) * | 1988-04-25 | 1989-12-15 | Phillips Petroleum Co | Expression of the hiv 24kda gag protein in methylotrophic yeasts |
| IL89992A0 (en) | 1988-04-25 | 1989-12-15 | Phillips Petroleum Co | Expression of human serum albumin in methylotrophic yeasts |
| NZ228774A (en) * | 1988-04-25 | 1991-05-28 | Phillips Petroleum Co | Hbv particles containing pres 2 proteins and recombinant processes for their production in yeast cells |
| IL90021A0 (en) * | 1988-04-25 | 1989-12-15 | Phillips Petroleum Co | Process for the production of interferon |
| IL89989A0 (en) * | 1988-04-25 | 1989-12-15 | Phillips Petroleum Co | Expression of human interleukin-2 in methylotrophic yeasts |
| AT390267B (de) * | 1988-06-08 | 1990-04-10 | Vogelbusch Gmbh | Verfahren zum markieren und identifizieren von industriell eingesetzten mikroorganismen, vorzugsweise hefestaemmen |
| US5102789A (en) * | 1989-03-15 | 1992-04-07 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production of epideramal growth factor in pichia pastoris yeast cells |
| US5204252A (en) * | 1989-02-08 | 1993-04-20 | Henkel Research Corporation | Candida tropicalis transformation system |
| WO1990009434A1 (en) * | 1989-02-13 | 1990-08-23 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production of superoxide dismutase in pichia pastoris yeast cells |
| CA2017176A1 (en) * | 1989-05-22 | 1990-11-22 | Yoshitomi Pharmaceutical Industries Ltd. | Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin |
| JPH0669365B2 (ja) * | 1989-05-22 | 1994-09-07 | 株式会社ミドリ十字 | アルブミン遺伝子を含むプラスミド、形質転換体、形質転換体の製造方法、アルブミンの製造方法 |
| US5369028A (en) * | 1990-04-03 | 1994-11-29 | The Salk Institute Biotechnology/Industrial Associates, Inc. | DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same |
| US6440681B1 (en) | 1990-04-03 | 2002-08-27 | Merck & Co., Inc. | Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors |
| US5258302A (en) * | 1990-07-03 | 1993-11-02 | The Salk Institute Biotechnology/Industrial Associates, Inc. | DNA for expression of aprotinin in methylotrophic yeast cells |
| US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| JPH06500470A (ja) * | 1990-09-04 | 1994-01-20 | メルク エンド カンパニー インコーポレーテッド | メチロトローフ酵母細胞におけるインスリン様成長因子の産生 |
| US5268273A (en) * | 1990-12-14 | 1993-12-07 | Phillips Petroleum Company | Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same |
| WO1992013951A1 (en) * | 1991-02-04 | 1992-08-20 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production of human serum albumin in methylotrophic yeast cells |
| CA2063890C (en) * | 1991-03-27 | 2007-03-13 | Masami Miura | Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism |
| AU661844B2 (en) * | 1991-04-01 | 1995-08-10 | Merck Sharp & Dohme Corp. | Genes which influence (pichia) proteolytic activity, and uses therefor |
| CA2058820C (en) * | 1991-04-25 | 2003-07-15 | Kotikanyad Sreekrishna | Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells |
| US5330901A (en) * | 1991-04-26 | 1994-07-19 | Research Corporation Technologies, Inc. | Expression of human serum albumin in Pichia pastoris |
| US5665600A (en) * | 1991-09-18 | 1997-09-09 | Research Corporation Technologies, Inc. | Pichia pastoris linear plasmids and DNA fragments thereof |
| WO1994020631A2 (en) * | 1993-03-03 | 1994-09-15 | E.I. Du Pont De Nemours And Company | Production of glycolate oxidase in methylotrophic yeast |
| EP0688361B1 (en) * | 1993-03-08 | 2004-10-13 | Merck & Co., Inc. | Human neuronal nicotinic acetylcholine receptor compositions and methods employing same |
| EP0696320B1 (en) * | 1993-04-20 | 2002-09-25 | Merck & Co., Inc. | Human n-methyl-d-aspartate receptor subunits, nucleic acids encoding same and uses therefor |
| JP3937445B2 (ja) * | 1993-05-28 | 2007-06-27 | ユニバーシテイ・オブ・アイオワ・リサーチ・フアウンデーシヨン | グリオキシル酸／アミノメチルホスホン酸ジアルキル混合物の製造法 |
| US5912122A (en) * | 1993-06-04 | 1999-06-15 | Sibia Neurosciences, Inc. | Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6 |
| US5521297A (en) | 1993-06-04 | 1996-05-28 | Salk Institute Biotechnology/Industrial Associates | Nucleic acids encoding human metabotropic glutamate receptors |
| US6001581A (en) | 1993-06-04 | 1999-12-14 | Sibia Neurosciences, Inc. | Cation-based bioassay using human metabotropic glutamate receptors |
| US6495343B1 (en) | 1993-06-18 | 2002-12-17 | The Salk Institute For Biological Studies | Cloning and recombinant production of CRF receptor(s) |
| US6638905B2 (en) * | 1993-06-18 | 2003-10-28 | The Salk Institute For Biological Studies | Cloning and recombinant production of CFR receptor(s) |
| GB2287941B (en) * | 1993-11-08 | 1998-01-28 | Salk Inst Biotech Ind | Human alpha2 neuronal nicotinic acetylcholine receptor compositions and methods employing same |
| CN1048254C (zh) * | 1993-12-09 | 2000-01-12 | 托马斯杰弗逊大学 | 用于将预定的改变引入靶基因中的化合物 |
| US5863894A (en) * | 1995-06-05 | 1999-01-26 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
| US5866542A (en) * | 1994-10-18 | 1999-02-02 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
| US5866543A (en) * | 1995-06-05 | 1999-02-02 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
| AU711405B2 (en) * | 1994-10-18 | 1999-10-14 | Dendreon Corporation | Nematode-extracted serine protease inhibitors and anticoagulant proteins |
| US5872098A (en) * | 1995-06-05 | 1999-02-16 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
| CA2202351A1 (en) * | 1994-10-18 | 1996-04-25 | George Phillip Vlasuk | Nematode-extracted serine protease inhibitors and anticoagulant proteins |
| US5945275A (en) * | 1994-10-18 | 1999-08-31 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
| JP3360830B2 (ja) | 1995-03-17 | 2003-01-07 | ノボザイムス アクティーゼルスカブ | 新規なエンドグルカナーゼ |
| US6485967B1 (en) | 1995-06-07 | 2002-11-26 | Merck & Co., Inc. | Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid |
| AU7719696A (en) * | 1995-10-18 | 1997-05-07 | New York Blood Center, Inc., The | Recombinant alpha-n-acetylgalactosaminidase enzyme |
| JP2000500014A (ja) * | 1995-11-09 | 2000-01-11 | ザイモジェネティクス，インコーポレイティド | ピヒア・メタノリカ中で異種ポリペプチドを生産するための組成物と方法 |
| US6001597A (en) * | 1995-11-09 | 1999-12-14 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| CA2246733C (en) | 1996-03-01 | 2010-07-20 | Novo Nordisk A/S | Use of a pharmaceutical composition comprising an appetite-suppressing peptide |
| US5731181A (en) * | 1996-06-17 | 1998-03-24 | Thomas Jefferson University | Chimeric mutational vectors having non-natural nucleotides |
| DK1632574T3 (da) | 1996-10-16 | 2011-02-28 | Zymogenetics Inc | Fibroblast-vækstfaktor homologer |
| MX9605082A (es) | 1996-10-24 | 1998-04-30 | Univ Autonoma De Nuevo Leon | Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano. |
| DK1115862T3 (da) | 1998-09-23 | 2009-11-16 | Zymogenetics Inc | Cytokinreceptor Zalpha11 |
| WO2000034474A2 (en) | 1998-12-07 | 2000-06-15 | Zymogenetics, Inc. | Growth factor homolog zvegf3 |
| US6780615B1 (en) | 1998-12-31 | 2004-08-24 | Genway Biotech Inc. | Production of recombinant monellin using methylotrophic yeast expression system |
| NZ512753A (en) | 1999-01-07 | 2003-10-31 | Zymogenetics Inc | Soluble receptor BR43x2 related proteins useful for inhibiting ztnf4 |
| US7833529B1 (en) | 1999-01-07 | 2010-11-16 | Zymogenetics, Inc. | Methods for inhibiting B lymphocyte proliferation with soluble ztnf4 receptor |
| ATE377076T1 (de) | 1999-03-09 | 2007-11-15 | Zymogenetics Inc | Humanes cytokin als ligand des zalpha rezeptors und dessen verwendungen |
| US7504253B2 (en) * | 1999-06-11 | 2009-03-17 | The Burnham Institute For Medical Research | Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof |
| ES2364086T3 (es) | 1999-07-07 | 2011-08-24 | Zymogenetics, Inc. | Receptor de citoquina humana. |
| EP1242600B1 (en) | 1999-12-23 | 2010-03-03 | ZymoGenetics, Inc. | Cytokine zcyto18 |
| EP1278852B1 (en) | 2000-05-11 | 2010-07-07 | ZymoGenetics, Inc. | Zsig33-like peptides |
| EP2258725A3 (en) | 2000-06-26 | 2014-09-17 | ZymoGenetics, L.L.C. | Cytokine receptor zcytor17 |
| US7625756B2 (en) * | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
| EP2322644A1 (en) | 2000-06-28 | 2011-05-18 | GlycoFi, Inc. | Methods for producing modified glycoproteins |
| US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
| US7598055B2 (en) * | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| US7863020B2 (en) * | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
| US8697394B2 (en) * | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
| EP1717316B1 (en) | 2000-06-30 | 2008-08-13 | ZymoGenetics, Inc. | Allelic variant of the Interferon-like protein ZCYTO21 |
| JP4092194B2 (ja) | 2000-06-30 | 2008-05-28 | フランダース インターユニバーシティ インスティテュート フォア バイオテクノロジー （ヴィーアイビー） | ピキアパストリス（ＰｉｃｈｉａＰａｓｔｏｒｉｓ）におけるタンパク質糖鎖修飾 |
| US7009045B2 (en) * | 2000-07-14 | 2006-03-07 | Archer-Daniels-Midland Company | Transformation systems for flavinogenic yeast |
| EP1736545A3 (en) | 2000-08-08 | 2007-03-28 | ZymoGenetics, Inc. | Soluble zcytor 11 cytokine receptors |
| JP2004533997A (ja) | 2001-02-20 | 2004-11-11 | ザイモジェネティクス，インコーポレイティド | Ｂｃｍａ及びｔａｃｉの両者を結合する抗体 |
| JP2004529640A (ja) | 2001-03-22 | 2004-09-30 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 第ｖｉｉ凝固因子誘導体 |
| EP1385866A4 (en) | 2001-04-05 | 2006-03-29 | Univ Nebraska | ALCOHOL OXIDASE-1 REGULATING NUCLEOTIDE SEQUENCES FOR HETEROLOGIC GENE EXPRESSION IN YEAST |
| EP2116259B1 (en) | 2001-05-24 | 2012-01-25 | ZymoGenetics, Inc. | TACI-immunoglobulin fusion proteins |
| DK1451322T3 (da) | 2001-11-05 | 2010-02-01 | Zymogenetics Inc | Il-21-antagonister |
| WO2003054155A2 (en) * | 2001-12-19 | 2003-07-03 | Bristol-Myers Squibb Company | Pichia pastoris formate dehydrogenase and uses therefor |
| US7494804B2 (en) | 2002-01-18 | 2009-02-24 | Zymogenetics, Inc. | Polynucleotide encoding cytokine receptor zcytor17 multimer |
| RU2360923C2 (ru) | 2002-01-18 | 2009-07-10 | Займоджинетикс, Инк. | Новый лиганд рецептора цитокина zcytor17 |
| US20060088834A1 (en) * | 2002-04-15 | 2006-04-27 | Gretchen Bain | Matrix analysis of gene expression in cells (magec) |
| WO2003089603A2 (en) | 2002-04-19 | 2003-10-30 | Zymogenetics, Inc. | Cytokine receptor |
| IL165717A0 (en) | 2002-06-26 | 2006-01-15 | Flanders Interuniversity Inst | A strain of methylotrophic yeast for producing proteins |
| DE60335602D1 (de) * | 2002-12-18 | 2011-02-17 | Roche Diagnostics Gmbh | Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität |
| DE60319333D1 (de) * | 2002-12-20 | 2008-04-10 | Roche Diagnostics Gmbh | Hitzelabile Desoxyribonuklease I-Varianten |
| US7332299B2 (en) * | 2003-02-20 | 2008-02-19 | Glycofi, Inc. | Endomannosidases in the modification of glycoproteins in eukaryotes |
| EP1460425A1 (en) * | 2003-03-17 | 2004-09-22 | Boehringer Mannheim Gmbh | Deglycosylated enzymes for conjugates |
| EP2251353B1 (en) | 2003-08-07 | 2013-03-06 | ZymoGenetics, Inc. | Homogeneous preparations of IL-29 |
| CN1863908B (zh) | 2003-09-09 | 2010-08-04 | 诺和诺德医疗保健公司 | 凝固因子ⅶ多肽 |
| CA2486195C (en) * | 2003-12-05 | 2012-03-06 | Stephan Glaser | Recombinantly expressed carboxypeptidase b and purification thereof |
| WO2005078074A2 (en) | 2004-02-13 | 2005-08-25 | Novozymes A/S | Protease variants |
| EP1732946B1 (en) | 2004-03-08 | 2011-07-27 | ZymoGenetics, Inc. | Dimeric fusion proteins and materials and methods for producing them |
| WO2006012644A2 (en) | 2004-07-29 | 2006-02-02 | Zymogenetics, Inc. | Use of il-28 and il-29 to treat cancer |
| AU2005268917B2 (en) | 2004-08-02 | 2010-05-20 | Basf Plant Science Gmbh | Method for isolation of transcription termination sequences |
| AU2006212807A1 (en) | 2005-02-08 | 2006-08-17 | Zymogenetics, Inc. | Anti-IL-20, anti-IL-22 and anti-IL-22RA antibodies and binding partners and methods of using in inflammation |
| DE602006020032D1 (de) | 2005-04-18 | 2011-03-24 | Novo Nordisk As | Il-21-varianten |
| EP3214095B1 (en) | 2005-05-12 | 2019-12-11 | ZymoGenetics, Inc. | Compositions and methods for modulating immune responses |
| US7393667B2 (en) * | 2005-05-31 | 2008-07-01 | Bristol-Myers Squibb Company | Stereoselective reduction process for the preparation of pyrrolotriazine compounds |
| AU2006278227B2 (en) | 2005-08-09 | 2011-10-20 | Ares Trading S.A. | Methods for the treatment and prevention of abnormal cell proliferation using TACI-fusion molecules |
| UA93516C2 (ru) * | 2005-08-09 | 2011-02-25 | Займоджинетикс, Инк. | Способы лечения b-клеточных злокачественных образований c помощью слитой молекулы taci-ig |
| ES2368500T3 (es) | 2005-08-16 | 2011-11-17 | Novo Nordisk A/S | Método para producir polipéptidos de insulina maduros. |
| US20090055942A1 (en) | 2005-09-14 | 2009-02-26 | Novo Nordisk Healthcare A/G | Human Coagulation Factor VII Polypeptides |
| US7981635B2 (en) * | 2005-09-14 | 2011-07-19 | Sanofi-Aventis Deutschland Gmbh | Cleavage of precursors of insulins by a variant of trypsin |
| US7855279B2 (en) | 2005-09-27 | 2010-12-21 | Amunix Operating, Inc. | Unstructured recombinant polymers and uses thereof |
| EP1931697B1 (en) | 2005-09-28 | 2010-09-15 | ZymoGenetics, Inc. | Il-17a and il-17f antagonists and methods of using the same |
| NZ572373A (en) * | 2006-05-15 | 2012-02-24 | Ares Trading Sa | Methods for treating rheumatoid arthritis using a taci-ig fusion molecule |
| ES2464282T3 (es) | 2006-09-27 | 2014-06-02 | Novo Nordisk A/S | Método para preparar polipéptidos de insulina madura |
| JP5967861B2 (ja) | 2007-04-03 | 2016-08-10 | オキシレイン ユーケー リミテッド | 分子のグリコシル化 |
| EP2164863A4 (en) | 2007-06-15 | 2010-07-28 | Univ Arkansas | METHOD FOR THE ADMINISTRATION OF MOLECULES IN CELLS USING A RICIN SUB-UNIT AND RELATED COMPOSITIONS THEREOF |
| JP5474809B2 (ja) | 2007-11-21 | 2014-04-16 | ロスキレ ユニバーシティ | 氷結合活性を備えたポリペプチド |
| CN101952309A (zh) * | 2007-12-21 | 2011-01-19 | Ifxa有限公司 | 蛋白酶抑制剂 |
| DK2245052T3 (en) * | 2008-01-25 | 2017-09-11 | Univ Aarhus | SELECTIVE EXOSITE INHIBITATION OF PAPP-A ACTIVITY AGAINST IGFBP-4 |
| WO2009114093A2 (en) | 2008-03-03 | 2009-09-17 | Abbott Laboratories | Methods for transforming yeast |
| WO2009120922A2 (en) | 2008-03-27 | 2009-10-01 | Zymogenetics, Inc. | Compositions and methods for inhibiting pdgfrbeta and vegf-a |
| WO2009139349A1 (ja) * | 2008-05-14 | 2009-11-19 | Ｂｉｏ－ｅｎｅｒｇｙ株式会社 | 酵母細胞に遺伝子を導入する方法およびそのためのベクター |
| EP2623112B1 (en) | 2008-06-27 | 2015-03-04 | Zymogenetics, Inc. | Soluble hybrid Fc gamma receptors and related methods |
| EA201100943A1 (ru) | 2008-12-16 | 2012-05-30 | Новартис Аг | Системы дрожжевого дисплея |
| CA2748314C (en) | 2009-02-03 | 2018-10-02 | Amunix Operating Inc. | Extended recombinant polypeptides and compositions comprising same |
| WO2010103038A1 (en) | 2009-03-11 | 2010-09-16 | Novo Nordisk A/S | Interleukin-21 variants having antagonistic binding to the il-21 receptor |
| AU2010272483B2 (en) | 2009-07-17 | 2016-07-21 | Omeros Corporation | MASP isoforms as inhibitors of complement activation |
| AU2010290131C1 (en) | 2009-08-24 | 2015-12-03 | Amunix Operating Inc. | Coagulation factor VII compositions and methods of making and using same |
| JP5990102B2 (ja) | 2009-09-29 | 2016-09-07 | ユニフェルシテイト ヘント | ホスホ−６−マンノースへのマンノース−１−ホスホ−６−マンノース結合の加水分解 |
| US20130053550A1 (en) | 2009-11-19 | 2013-02-28 | Oxyrane Uk Limited | Yeast strains producing mammalian-like complex n-glycans |
| JP5850848B2 (ja) | 2009-11-25 | 2016-02-03 | ノヴォ ノルディスク アー／エス | ポリペプチドの製造方法 |
| WO2011067283A1 (en) | 2009-12-01 | 2011-06-09 | Novo Nordisk A/S | Novel peptidyl alpha-hydroxyglycine alpha-amidating lyases |
| CA2791841C (en) | 2010-03-05 | 2023-01-03 | Rigshospitalet | Chimeric inhibitor molecules of complement activation |
| EP2580238A1 (en) | 2010-06-09 | 2013-04-17 | Zymogenetics, Inc. | Dimeric vstm3 fusion proteins and related compositions and methods |
| JP6131190B2 (ja) | 2010-09-29 | 2017-05-17 | オキシレイン ユーケー リミテッド | リン酸化ｎ−グリカンの脱マンノシル化 |
| SG189108A1 (en) | 2010-09-29 | 2013-05-31 | Oxyrane Uk Ltd | Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins |
| WO2013098651A1 (en) | 2011-12-30 | 2013-07-04 | Oxyrane Uk Limited | Methods and materials for reducing degradation of recombinant proteins |
| CN104220602B (zh) | 2012-02-01 | 2018-10-30 | 合成基因组股份有限公司 | 用于合成错误最小化核酸分子的材料和方法 |
| US11787844B2 (en) | 2012-02-09 | 2023-10-17 | Var2 Pharmaceuticals Aps | Targeting of chondroitin sulfate glycans |
| DK2822577T3 (en) | 2012-02-15 | 2019-04-01 | Bioverativ Therapeutics Inc | RECOMBINANT FACTOR VIII PROTEINS |
| ES2771208T3 (es) | 2012-02-15 | 2020-07-06 | Bioverativ Therapeutics Inc | Composiciones de factor VIII y métodos de preparación y uso de las mismas |
| AU2013234042B2 (en) | 2012-03-15 | 2017-11-02 | Oxyrane Uk Limited | Methods and materials for treatment of Pompe's disease |
| KR101958234B1 (ko) * | 2012-05-09 | 2019-03-14 | 엘지이노텍 주식회사 | 보이스 코일 모터 |
| US20150307865A1 (en) | 2012-10-15 | 2015-10-29 | Novo Nordisk Health Care Ag | Coagulation factor vii polypeptides |
| US20190040368A1 (en) | 2013-03-05 | 2019-02-07 | Oxyrane Uk Limited | Production of catalytically active type i sulfatase |
| AU2014234962C1 (en) | 2013-03-21 | 2019-01-17 | Evonik Operations Gmbh | Purification of triple helical proteins |
| EP3004156A1 (en) | 2013-06-07 | 2016-04-13 | Novo Nordisk A/S | Method for making mature insulin polypeptides |
| WO2014202089A2 (en) | 2013-06-18 | 2014-12-24 | Roskilde Universitet | Variants of anti-freeze polypeptides |
| WO2015023891A2 (en) | 2013-08-14 | 2015-02-19 | Biogen Idec Ma Inc. | Factor viii-xten fusions and uses thereof |
| CN105764920B (zh) | 2013-09-09 | 2020-07-24 | 联邦科学工业研究组织 | 修饰的细菌胶原蛋白样蛋白 |
| WO2016017693A1 (ja) * | 2014-07-30 | 2016-02-04 | 第一三共株式会社 | タンパク質の改良高分泌生産方法 |
| WO2017024060A1 (en) | 2015-08-03 | 2017-02-09 | Biogen Ma Inc. | Factor ix fusion proteins and methods of making and using same |
| EP3328881B1 (en) | 2015-09-08 | 2019-08-28 | Theripion, Inc. | Apoa-1 fusion polypeptides and related compositions and methods |
| WO2018136163A2 (en) | 2016-12-09 | 2018-07-26 | Theripion, Inc. | Tandem apoa-1 fusion polypeptides |
| JP7012663B2 (ja) * | 2016-12-15 | 2022-02-14 | 株式会社カネカ | 新規宿主細胞及びそれを用いた目的タンパク質の製造方法 |
| CA3070272A1 (en) | 2017-07-20 | 2019-01-24 | Aptevo Research And Development Llc | Antigen binding proteins binding to 5t4 and 4-1bb and related compositions and methods |
| RU2756100C1 (ru) | 2017-12-20 | 2021-09-28 | Харбор Байомед (Шанхай) Ко., Лтд | Антитела, связывающие ctla-4, и их применение |
| US10150801B1 (en) | 2017-12-27 | 2018-12-11 | Imunami Laboratories Pte. Ltd. | Recombinant polypeptides and methods of use thereof |
| WO2019132765A1 (en) | 2017-12-27 | 2019-07-04 | Imunami Laboratories Pte. Ltd. | Recombinant polypeptides and methods of use thereof |
| DE202019005554U1 (de) * | 2018-03-06 | 2021-01-18 | Joywell Foods, Inc. | Rekombinant exprimierte geschmacksmodifizierende Polypeptide und Präparate und Formulierungen, die diese enthalten |
| NZ769677A (en) | 2018-05-18 | 2024-07-05 | Bioverativ Therapeutics Inc | Methods of treating hemophilia a |
| US20210278406A1 (en) | 2018-07-13 | 2021-09-09 | Varct Diagnostic Aps | Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa |
| CN111378585B (zh) * | 2018-12-28 | 2023-06-16 | 丰益(上海)生物技术研发中心有限公司 | 用于表达外源基因的毕赤酵母突变株 |
| WO2021040610A1 (en) | 2019-08-26 | 2021-03-04 | Imunami Laboratories Pte. Ltd. | Recombinant polypeptides and methods of use thereof |
| US10675332B1 (en) | 2019-08-26 | 2020-06-09 | Imunami Laboratories Pte. Ltd. | Recombinant polypeptides and methods of use thereof |
| WO2021262081A1 (en) | 2020-06-22 | 2021-12-30 | Imunami Laboratories Pte. Ltd. | Recombinant polypeptides and combinations for use in the treatment of cancer |
| WO2022178090A2 (en) | 2021-02-19 | 2022-08-25 | Theripion, Inc. | Dnase fusion polypeptides and related compositions and methods |
| WO2024148328A2 (en) | 2023-01-06 | 2024-07-11 | Aptevo Research And Development Llc | Bispecific pd-l1 and cd40 binding molecules and uses thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4530901A (en) * | 1980-01-08 | 1985-07-23 | Biogen N.V. | Recombinant DNA molecules and their use in producing human interferon-like polypeptides |
| US4414329A (en) * | 1980-01-15 | 1983-11-08 | Phillips Petroleum Company | Biochemical conversions by yeast fermentation at high cell densities |
| JPS57206699A (en) * | 1981-06-10 | 1982-12-18 | Takeda Chem Ind Ltd | Plasmid patm 3 and its preparation |
| US4837148A (en) * | 1984-10-30 | 1989-06-06 | Phillips Petroleum Company | Autonomous replication sequences for yeast strains of the genus pichia |
| US4855231A (en) * | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4879231A (en) * | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
-
1985
- 1985-10-25 US US06/791,013 patent/US4882279A/en not_active Expired - Lifetime
-
1986
- 1986-07-29 CA CA000514838A patent/CA1339209C/en not_active Expired - Lifetime
- 1986-10-06 IE IE263786A patent/IE68454B1/en not_active IP Right Cessation
- 1986-10-06 NZ NZ217809A patent/NZ217809A/en unknown
- 1986-10-07 ZA ZA867650A patent/ZA867650B/xx unknown
- 1986-10-08 MX MX003971A patent/MX168390B/es unknown
- 1986-10-08 IN IN731/CAL/86A patent/IN166443B/en unknown
- 1986-10-09 PH PH34343A patent/PH25990A/en unknown
- 1986-10-10 IL IL80286A patent/IL80286A/xx not_active IP Right Cessation
- 1986-10-14 AU AU63882/86A patent/AU581107B2/en not_active Expired
- 1986-10-20 KR KR1019860008825A patent/KR930002738B1/ko not_active IP Right Cessation
- 1986-10-20 JP JP61249403A patent/JP2614215B2/ja not_active Expired - Lifetime
- 1986-10-22 EG EG659/86A patent/EG17985A/xx active
- 1986-10-23 AT AT93105818T patent/ATE197962T1/de not_active IP Right Cessation
- 1986-10-23 EP EP86114700A patent/EP0226752B1/en not_active Expired - Lifetime
- 1986-10-23 DE DE3650749T patent/DE3650749T2/de not_active Expired - Lifetime
- 1986-10-23 YU YU180386A patent/YU46758B/sh unknown
- 1986-10-23 ES ES86114700T patent/ES2061433T3/es not_active Expired - Lifetime
- 1986-10-23 DE DE3689411T patent/DE3689411T2/de not_active Expired - Lifetime
- 1986-10-23 AT AT86114700T patent/ATE98695T1/de not_active IP Right Cessation
- 1986-10-23 EP EP93105818A patent/EP0560401B1/en not_active Expired - Lifetime
- 1986-10-23 ES ES93105818T patent/ES2152233T3/es not_active Expired - Lifetime
- 1986-10-24 DK DK510786A patent/DK510786A/da not_active Application Discontinuation
- 1986-10-24 CN CN198686107108A patent/CN86107108A/zh active Pending
- 1986-10-24 NO NO864275A patent/NO176923C/no unknown
- 1986-10-24 PL PL1986262030A patent/PL154843B1/pl unknown
- 1986-10-24 DD DD29558586A patent/DD255544A5/de unknown
- 1986-10-24 HU HU864480A patent/HU208552B/hu not_active IP Right Cessation
- 1986-10-24 PT PT83618A patent/PT83618B/pt not_active IP Right Cessation
- 1986-10-24 FI FI864319A patent/FI98931C/fi not_active IP Right Cessation
-
1988
- 1988-04-15 AU AU14699/88A patent/AU593436B2/en not_active Expired
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PH25990A (en) | Site selective genomic modification of yeast of the genus pichia | |
| US5166329A (en) | DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia | |
| US4885242A (en) | Genes from pichia histidine pathway and uses thereof | |
| AU601768B2 (en) | Yeast production of human tumor necrosis factor | |
| FI81379B (fi) | Anvaendning av kluyveromyces-jaest saosom vaerd foer transformering och uttryckning av fraemmande gener. | |
| EP0460716B2 (en) | Yeast expression systems with vectors having GAPDH promoters, and synthesis of HBsAg | |
| FI95285B (fi) | 2 m-plasmidivektori, menetelmä sen valmistamiseksi sekä sen käyttö | |
| US4895800A (en) | Yeast production of hepatitis B surface antigen | |
| US5135868A (en) | Cultures of yeast of the genus Pichia altered by site selective genomic modification | |
| IE852472L (en) | Autonomous yeast replication sequences | |
| FI104264B (fi) | Menetelmä sekundääristen metaboliittien tuoton parantamiseksi käyttämällä rykelmöityjä biosynteettisiä geenejä | |
| EP0225078A2 (en) | Composite expression cassettes for fungal transformation | |
| US5616474A (en) | K. lactis transaldolase gene promoter and use thereof | |
| US7973215B2 (en) | Method for the introduction of a heterologous polynucleotide into a mushroom | |
| Obraztsova et al. | Genetic damage following introduction of DNA in Phycomyces | |
| JPH0576375A (ja) | 改良された酵母ベクター | |
| EP0549062B1 (en) | A eukaryotic expression system | |
| WO1995009914A1 (fr) | Vecteur de multiclonage, vecteur d'expression, et production d'une proteine exogene au moyen dudit vecteur d'expression | |
| IE83234B1 (en) | DNA fragment encoding an AOX | |
| US20050289666A1 (en) | Starch-inducible promoters, recombinant gene constructs, and methods of regulating gene expression | |
| JPH03262487A (ja) | ヒト血清アルブミンの安定な製造方法 | |
| JPH08511161A (ja) | 酵母プロモーターおよびその使用 |