EP1385866A4 - Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast - Google Patents

Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast

Info

Publication number
EP1385866A4
EP1385866A4 EP02764010A EP02764010A EP1385866A4 EP 1385866 A4 EP1385866 A4 EP 1385866A4 EP 02764010 A EP02764010 A EP 02764010A EP 02764010 A EP02764010 A EP 02764010A EP 1385866 A4 EP1385866 A4 EP 1385866A4
Authority
EP
European Patent Office
Prior art keywords
yeast
gene expression
nucleotide sequences
heterologous gene
alcohol oxidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02764010A
Other languages
German (de)
French (fr)
Other versions
EP1385866A2 (en
Inventor
Mehmet Inan
Michael M Meagher
Andrew K Benson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Nebraska
Original Assignee
University of Nebraska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Nebraska filed Critical University of Nebraska
Publication of EP1385866A2 publication Critical patent/EP1385866A2/en
Publication of EP1385866A4 publication Critical patent/EP1385866A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
EP02764010A 2001-04-05 2002-04-05 Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast Withdrawn EP1385866A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28186101P 2001-04-05 2001-04-05
US281861P 2001-04-05
PCT/US2002/012851 WO2002081650A2 (en) 2001-04-05 2002-04-05 Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast

Publications (2)

Publication Number Publication Date
EP1385866A2 EP1385866A2 (en) 2004-02-04
EP1385866A4 true EP1385866A4 (en) 2006-03-29

Family

ID=23079073

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02764010A Withdrawn EP1385866A4 (en) 2001-04-05 2002-04-05 Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast

Country Status (7)

Country Link
US (2) US6699691B2 (en)
EP (1) EP1385866A4 (en)
JP (1) JP2005514001A (en)
AU (1) AU2002307504B2 (en)
CA (1) CA2442935A1 (en)
MX (1) MXPA03009059A (en)
WO (1) WO2002081650A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT501955B1 (en) * 2005-02-23 2007-08-15 Univ Graz Tech MUTED AOX1 PROMOTERS
US8222386B2 (en) * 2006-10-10 2012-07-17 Keck Graduate Institute P. pastoris ADH promoter and use thereof to direct expression of proteins
AT504588B1 (en) * 2006-11-16 2008-08-15 Univ Graz Tech Nucleic Acid Promoter
US7892792B2 (en) * 2008-06-27 2011-02-22 Indian Institute Of Science Cells expressing Pichia cytochrome C
US11518797B2 (en) 2014-11-11 2022-12-06 Clara Foods Co. Methods and compositions for egg white protein production
US11643663B2 (en) 2018-04-16 2023-05-09 Akdeniz Universitesi Determination of regulatory DNA regions of alcohol dehydrogenase 3 (ADH3) promoter and design of synthetic promoters for recombinant protein production
KR20220034848A (en) 2019-07-11 2022-03-18 클라라 푸드즈 컴퍼니 Protein composition and edible products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
EP0510693A2 (en) * 1991-04-25 1992-10-28 Research Corporation Technologies, Inc. Expression of human serum albumin in pichia pastoris
WO1997017450A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica
US5641661A (en) * 1993-03-25 1997-06-24 Biosource Technologies, Inc. Pichia pastoris alcohol oxidase ZZA1 and ZZA2 regulatory regions for heterologous gene expression

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4782137A (en) 1984-01-24 1988-11-01 Immunex Corporation Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
US4703004A (en) 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US5032516A (en) 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US5166329A (en) 1985-10-25 1992-11-24 Phillips Petroleum Company DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
US5594115A (en) 1990-04-09 1997-01-14 Pharmacia & Upjohn Company Process of purifying recombinant proteins and compounds useful in such process
JPH06189769A (en) * 1992-10-30 1994-07-12 Green Cross Corp:The Variant aox2 promoter, vector carrying the promoter, transformant and production of foreign protein
US6033898A (en) 1992-12-30 2000-03-07 Abbott Laboratories Enhanced yeast expression using regulatory control sequences from yeast sorbitol dehydrogenase gene
US5500483A (en) 1993-03-25 1996-03-19 Biosource Technologies, Inc. Pichia pastoris alcohol oxidase ZZA1 regulatory region for heterologous gene expression
EP0852625A2 (en) * 1995-09-25 1998-07-15 Chiron Corporation Pichia secretory leader for protein expression
US5935824A (en) 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
EP0510693A2 (en) * 1991-04-25 1992-10-28 Research Corporation Technologies, Inc. Expression of human serum albumin in pichia pastoris
US5641661A (en) * 1993-03-25 1997-06-24 Biosource Technologies, Inc. Pichia pastoris alcohol oxidase ZZA1 and ZZA2 regulatory regions for heterologous gene expression
WO1997017450A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 24 January 1995 (1995-01-24), "Nucleotide sequence 4 from patent number US4808537.", XP002365843, retrieved from EBI accession no. EMBL:I01662 Database accession no. I01662 *
DATABASE Geneseq [online] 17 March 1993 (1993-03-17), "AOX1 regulatory region.", XP002356971, retrieved from EBI accession no. GSN:AAQ29693 Database accession no. AAQ29693 *
DATABASE Geneseq [online] 2 October 1991 (1991-10-02), "Sequence encoding alcohol oxidase regulator.", XP002365842, retrieved from EBI accession no. GSN:AAN60967 Database accession no. AAN60967 *

Also Published As

Publication number Publication date
US20040137591A1 (en) 2004-07-15
US20030044947A1 (en) 2003-03-06
MXPA03009059A (en) 2004-10-15
EP1385866A2 (en) 2004-02-04
CA2442935A1 (en) 2002-10-17
JP2005514001A (en) 2005-05-19
US6699691B2 (en) 2004-03-02
AU2002307504B2 (en) 2006-10-26
WO2002081650A3 (en) 2003-03-27
WO2002081650A2 (en) 2002-10-17

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Legal Events

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RIN1 Information on inventor provided before grant (corrected)

Inventor name: BENSON, ANDREW, K.

Inventor name: MEAGHER, MICHAEL, M.

Inventor name: INAN, MEHMET

A4 Supplementary search report drawn up and despatched

Effective date: 20060214

17Q First examination report despatched

Effective date: 20060915

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Effective date: 20091103