LT3686B - Process for preparing fused proteins, gene structure, process for transformation of hybride and streptomycetes cells - Google Patents
Process for preparing fused proteins, gene structure, process for transformation of hybride and streptomycetes cells Download PDFInfo
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- LT3686B LT3686B LTIP1523A LTIP1523A LT3686B LT 3686 B LT3686 B LT 3686B LT IP1523 A LTIP1523 A LT IP1523A LT IP1523 A LTIP1523 A LT IP1523A LT 3686 B LT3686 B LT 3686B
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- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- PTMHPRAIXMAOOB-UHFFFAOYSA-L phosphoramidate Chemical compound NP([O-])([O-])=O PTMHPRAIXMAOOB-UHFFFAOYSA-L 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 108010066381 preproinsulin Proteins 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- NHXLMOGPVYXJNR-ATOGVRKGSA-N somatostatin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N1)[C@@H](C)O)NC(=O)CNC(=O)[C@H](C)N)C(O)=O)=O)[C@H](O)C)C1=CC=CC=C1 NHXLMOGPVYXJNR-ATOGVRKGSA-N 0.000 description 1
- 229960000553 somatostatin Drugs 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229930188070 thiostrepton Natural products 0.000 description 1
- NSFFHOGKXHRQEW-AIHSUZKVSA-N thiostrepton Chemical compound C([C@]12C=3SC=C(N=3)C(=O)N[C@H](C(=O)NC(/C=3SC[C@@H](N=3)C(=O)N[C@H](C=3SC=C(N=3)C(=O)N[C@H](C=3SC=C(N=3)[C@H]1N=1)[C@@H](C)OC(=O)C3=CC(=C4C=C[C@H]([C@@H](C4=N3)O)N[C@H](C(N[C@@H](C)C(=O)NC(=C)C(=O)N[C@@H](C)C(=O)N2)=O)[C@@H](C)CC)[C@H](C)O)[C@](C)(O)[C@@H](C)O)=C\C)[C@@H](C)O)CC=1C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-AIHSUZKVSA-N 0.000 description 1
- 229940063214 thiostrepton Drugs 0.000 description 1
- NSFFHOGKXHRQEW-OFMUQYBVSA-N thiostrepton A Natural products CC[C@H](C)[C@@H]1N[C@@H]2C=Cc3c(cc(nc3[C@H]2O)C(=O)O[C@H](C)[C@@H]4NC(=O)c5csc(n5)[C@@H](NC(=O)[C@H]6CSC(=N6)C(=CC)NC(=O)[C@@H](NC(=O)c7csc(n7)[C@]8(CCC(=N[C@@H]8c9csc4n9)c%10nc(cs%10)C(=O)NC(=C)C(=O)NC(=C)C(=O)N)NC(=O)[C@H](C)NC(=O)C(=C)NC(=O)[C@H](C)NC1=O)[C@@H](C)O)[C@](C)(O)[C@@H](C)O)[C@H](C)O NSFFHOGKXHRQEW-OFMUQYBVSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 108010078580 tyrosylleucine Proteins 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Diabetes (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4012818A DE4012818A1 (de) | 1990-04-21 | 1990-04-21 | Verfahren zur herstellung von fremdproteinen in streptomyceten |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LTIP1523A LTIP1523A (en) | 1995-06-26 |
| LT3686B true LT3686B (en) | 1996-01-25 |
Family
ID=6404857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTIP1523A LT3686B (en) | 1990-04-21 | 1993-12-03 | Process for preparing fused proteins, gene structure, process for transformation of hybride and streptomycetes cells |
Country Status (29)
| Country | Link |
|---|---|
| EP (1) | EP0453969B1 (ru) |
| JP (1) | JP3319605B2 (ru) |
| KR (1) | KR0168669B1 (ru) |
| CN (1) | CN1049248C (ru) |
| AT (1) | ATE142263T1 (ru) |
| AU (1) | AU630287B2 (ru) |
| BR (1) | BR9101587A (ru) |
| CA (1) | CA2040810C (ru) |
| CZ (1) | CZ285440B6 (ru) |
| DE (2) | DE4012818A1 (ru) |
| DK (1) | DK0453969T3 (ru) |
| ES (1) | ES2093043T3 (ru) |
| FI (1) | FI911882A (ru) |
| GR (1) | GR3021040T3 (ru) |
| HR (1) | HRP940770A2 (ru) |
| HU (1) | HU210358B (ru) |
| IE (1) | IE911322A1 (ru) |
| IL (1) | IL97903A0 (ru) |
| LT (1) | LT3686B (ru) |
| LV (1) | LV10494B (ru) |
| NO (1) | NO911557L (ru) |
| NZ (1) | NZ237882A (ru) |
| PL (2) | PL169596B1 (ru) |
| PT (1) | PT97427B (ru) |
| RU (1) | RU2055892C1 (ru) |
| SK (1) | SK110191A3 (ru) |
| TW (1) | TW213487B (ru) |
| YU (1) | YU48435B (ru) |
| ZA (1) | ZA912937B (ru) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI103805B (fi) * | 1990-09-05 | 1999-09-30 | Hoechst Ag | Menetelmä preproinsuliinien hydroloimiseksi |
| DK0600372T3 (da) | 1992-12-02 | 1997-08-11 | Hoechst Ag | Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer. |
| ES2161726T3 (es) * | 1993-04-27 | 2001-12-16 | Hoechst Ag | Formas amorfas monoesfericas de derivados de insulina. |
| DE4405179A1 (de) * | 1994-02-18 | 1995-08-24 | Hoechst Ag | Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken |
| CN1061375C (zh) * | 1996-07-19 | 2001-01-31 | 中国科学院上海生物工程研究中心 | 利用异源启动子在链霉菌中表达透明颤菌血红蛋白 |
| DK0821006T3 (da) | 1996-07-26 | 2004-08-16 | Aventis Pharma Gmbh | Insulinderivater med öget zinkbinding |
| DE19825447A1 (de) | 1998-06-06 | 1999-12-09 | Hoechst Marion Roussel De Gmbh | Neue Insulinanaloga mit erhöhter Zinkbildung |
| WO2004106525A1 (fr) * | 2003-06-03 | 2004-12-09 | Shanghai Centre Of Research & Development Of New Drugs | Proteine de fusion pouvant etre exprimee tres efficacement et son procede de production |
| RU2395296C1 (ru) * | 2009-02-19 | 2010-07-27 | Общество С Ограниченной Ответственностью "Концерн О3" | Способ получения препарата проинсулина для перорального применения |
| CN104818291A (zh) * | 2015-05-08 | 2015-08-05 | 江南大学 | 一种链霉菌重组表达载体的构建及应用 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4366246A (en) | 1977-11-08 | 1982-12-28 | Genentech, Inc. | Method for microbial polypeptide expression |
| EP0161629A1 (de) | 1984-05-17 | 1985-11-21 | Hoechst Aktiengesellschaft | Signalpeptid für die Exkretion von Peptiden in Streptomyceten |
| EP0290005A2 (de) | 1987-05-06 | 1988-11-09 | Andreas Dr. Plückthun | Verfahren zur Herstellung eines genetisch codierbaren Polypeptids |
| EP0289936A2 (de) | 1987-05-05 | 1988-11-09 | Hoechst Aktiengesellschaft | Verfahren zur Herstellung von Fremdproteinen in Streptomyceten |
| EP0292763A2 (de) | 1987-05-19 | 1988-11-30 | Hoechst Aktiengesellschaft | Gentechnologisches Verfahren zur Herstellung von Angiogeninen |
| EP0367163A2 (de) | 1988-11-03 | 1990-05-09 | Hoechst Aktiengesellschaft | Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3707150A1 (de) * | 1987-03-06 | 1988-09-15 | Hoechst Ag | Tendamistat-derivate |
| DE3843713A1 (de) * | 1988-04-25 | 1989-11-02 | Henkel Kgaa | Verwendung von calcinierten hydrotalciten als katalysatoren fuer die ethoxylierung bzw. propoxylierung |
-
1990
- 1990-04-21 DE DE4012818A patent/DE4012818A1/de not_active Withdrawn
-
1991
- 1991-04-18 DE DE59108128T patent/DE59108128D1/de not_active Expired - Lifetime
- 1991-04-18 ES ES91106268T patent/ES2093043T3/es not_active Expired - Lifetime
- 1991-04-18 EP EP91106268A patent/EP0453969B1/de not_active Expired - Lifetime
- 1991-04-18 AT AT91106268T patent/ATE142263T1/de not_active IP Right Cessation
- 1991-04-18 DK DK91106268.5T patent/DK0453969T3/da active
- 1991-04-18 YU YU69691A patent/YU48435B/sh unknown
- 1991-04-18 SK SK1101-91A patent/SK110191A3/sk unknown
- 1991-04-18 CZ CS911101A patent/CZ285440B6/cs not_active IP Right Cessation
- 1991-04-18 FI FI911882A patent/FI911882A/fi not_active Application Discontinuation
- 1991-04-19 BR BR919101587A patent/BR9101587A/pt not_active Application Discontinuation
- 1991-04-19 IE IE132291A patent/IE911322A1/en unknown
- 1991-04-19 NO NO91911557A patent/NO911557L/no unknown
- 1991-04-19 PL PL91309698A patent/PL169596B1/pl unknown
- 1991-04-19 KR KR1019910006250A patent/KR0168669B1/ko not_active IP Right Cessation
- 1991-04-19 IL IL97903A patent/IL97903A0/xx unknown
- 1991-04-19 AU AU75154/91A patent/AU630287B2/en not_active Expired
- 1991-04-19 NZ NZ237882A patent/NZ237882A/en unknown
- 1991-04-19 CA CA002040810A patent/CA2040810C/en not_active Expired - Lifetime
- 1991-04-19 RU SU914895292A patent/RU2055892C1/ru active
- 1991-04-19 PL PL91289953A patent/PL169178B1/pl unknown
- 1991-04-19 HU HU911302A patent/HU210358B/hu unknown
- 1991-04-19 PT PT97427A patent/PT97427B/pt not_active IP Right Cessation
- 1991-04-19 ZA ZA912937A patent/ZA912937B/xx unknown
- 1991-04-20 JP JP11700691A patent/JP3319605B2/ja not_active Expired - Lifetime
- 1991-04-20 CN CN91102541A patent/CN1049248C/zh not_active Expired - Lifetime
- 1991-05-28 TW TW080104141A patent/TW213487B/zh active
-
1993
- 1993-05-04 LV LVP-93-283A patent/LV10494B/xx unknown
- 1993-12-03 LT LTIP1523A patent/LT3686B/lt unknown
-
1994
- 1994-10-25 HR HRP-696/91A patent/HRP940770A2/hr not_active Application Discontinuation
-
1996
- 1996-09-13 GR GR960402399T patent/GR3021040T3/el unknown
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4366246A (en) | 1977-11-08 | 1982-12-28 | Genentech, Inc. | Method for microbial polypeptide expression |
| EP0161629A1 (de) | 1984-05-17 | 1985-11-21 | Hoechst Aktiengesellschaft | Signalpeptid für die Exkretion von Peptiden in Streptomyceten |
| EP0289936A2 (de) | 1987-05-05 | 1988-11-09 | Hoechst Aktiengesellschaft | Verfahren zur Herstellung von Fremdproteinen in Streptomyceten |
| EP0290005A2 (de) | 1987-05-06 | 1988-11-09 | Andreas Dr. Plückthun | Verfahren zur Herstellung eines genetisch codierbaren Polypeptids |
| EP0292763A2 (de) | 1987-05-19 | 1988-11-30 | Hoechst Aktiengesellschaft | Gentechnologisches Verfahren zur Herstellung von Angiogeninen |
| EP0367163A2 (de) | 1988-11-03 | 1990-05-09 | Hoechst Aktiengesellschaft | Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten |
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