CA2040810A1 - Process for producing foreign proteins in streptomyces - Google Patents

Process for producing foreign proteins in streptomyces

Info

Publication number
CA2040810A1
CA2040810A1 CA002040810A CA2040810A CA2040810A1 CA 2040810 A1 CA2040810 A1 CA 2040810A1 CA 002040810 A CA002040810 A CA 002040810A CA 2040810 A CA2040810 A CA 2040810A CA 2040810 A1 CA2040810 A1 CA 2040810A1
Authority
CA
Canada
Prior art keywords
streptomyces
foreign proteins
producing foreign
producing
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002040810A
Other languages
French (fr)
Other versions
CA2040810C (en
Inventor
Klaus-Peter Koller
Gunther Riess
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of CA2040810A1 publication Critical patent/CA2040810A1/en
Application granted granted Critical
Publication of CA2040810C publication Critical patent/CA2040810C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Process for producing foreign proteins in streptomyces Genetic structures which code for the signal sequence and about the first ten amino acids of tendamistat as well as a desired protein are expressed in stregtomyces host cells with a high yield, and the fusion proteins are secreted into the medium.
CA002040810A 1990-04-21 1991-04-19 Process for producing foreign proteins in streptomyces Expired - Lifetime CA2040810C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP4012818.0 1990-04-21
DE4012818A DE4012818A1 (en) 1990-04-21 1990-04-21 METHOD FOR THE PRODUCTION OF FOREIGN PROTEINS IN STREPTOMYCETES

Publications (2)

Publication Number Publication Date
CA2040810A1 true CA2040810A1 (en) 1991-10-22
CA2040810C CA2040810C (en) 2001-07-24

Family

ID=6404857

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002040810A Expired - Lifetime CA2040810C (en) 1990-04-21 1991-04-19 Process for producing foreign proteins in streptomyces

Country Status (29)

Country Link
EP (1) EP0453969B1 (en)
JP (1) JP3319605B2 (en)
KR (1) KR0168669B1 (en)
CN (1) CN1049248C (en)
AT (1) ATE142263T1 (en)
AU (1) AU630287B2 (en)
BR (1) BR9101587A (en)
CA (1) CA2040810C (en)
CZ (1) CZ285440B6 (en)
DE (2) DE4012818A1 (en)
DK (1) DK0453969T3 (en)
ES (1) ES2093043T3 (en)
FI (1) FI911882A (en)
GR (1) GR3021040T3 (en)
HR (1) HRP940770A2 (en)
HU (1) HU210358B (en)
IE (1) IE911322A1 (en)
IL (1) IL97903A0 (en)
LT (1) LT3686B (en)
LV (1) LV10494B (en)
NO (1) NO911557L (en)
NZ (1) NZ237882A (en)
PL (2) PL169596B1 (en)
PT (1) PT97427B (en)
RU (1) RU2055892C1 (en)
SK (1) SK110191A3 (en)
TW (1) TW213487B (en)
YU (1) YU48435B (en)
ZA (1) ZA912937B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100188800B1 (en) * 1990-09-05 1999-06-01 이센브룩, 라피세 Enzymatic process for the conversion of preproinsulins into insulins
SG46612A1 (en) 1992-12-02 1998-02-20 Hoechst Ag Process for obtaining proinsulin in processing correctly linked cystine bridges
DE59409816D1 (en) * 1993-04-27 2001-09-13 Hoechst Ag Amorphous monospheric forms of insulin derivatives
DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
CN1061375C (en) * 1996-07-19 2001-01-31 中国科学院上海生物工程研究中心 Using allogeneic promoter to express transparent Tremellineae haemoglobin in streptomycete
EP0821006B1 (en) 1996-07-26 2004-04-21 Aventis Pharma Deutschland GmbH Derivatives of insulin having enhanced zinc binding activity
DE19825447A1 (en) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh New insulin analogues with increased zinc formation
US7795384B2 (en) 2003-06-03 2010-09-14 Shanghai Centre Of Research & Development Of New Drugs Fusion protein suitable for high efficiency expression and the production method thereof
RU2395296C1 (en) * 2009-02-19 2010-07-27 Общество С Ограниченной Ответственностью "Концерн О3" Method for making oral proinsulin preparation
CN104818291A (en) * 2015-05-08 2015-08-05 江南大学 Construction and application of streptomycete recombinant expression vector

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
DE3418274A1 (en) 1984-05-17 1985-11-21 Hoechst Ag, 6230 Frankfurt SIGNAL PEPTIDE FOR THE EXCRETION OF PEPTIDES IN STREPTOMYCETS
DE3707150A1 (en) * 1987-03-06 1988-09-15 Hoechst Ag TENDAMISTAT DERIVATIVES
DE3714866A1 (en) * 1987-05-05 1988-11-24 Hoechst Ag METHOD FOR THE PRODUCTION OF FOREIGN PROTEINS IN STREPTOMYCETES
DE3715033A1 (en) 1987-05-06 1988-11-17 Hoechst Ag METHOD FOR ISOLATING FUSION PROTEINS
DE3716722A1 (en) 1987-05-19 1988-12-01 Hoechst Ag GENE TECHNOLOGICAL METHOD FOR PRODUCING ANGIOGENINES
DE3843713A1 (en) * 1988-04-25 1989-11-02 Henkel Kgaa USE OF CALCINATED HYDROTALCITES AS CATALYSTS FOR ETHOXYLATION OR PROPOXYLATION
ATE132531T1 (en) * 1988-11-03 1996-01-15 Hoechst Ag METHOD FOR PRODUCING AN INSULIN PREPRODUCT IN STREPTOMYCETES

Also Published As

Publication number Publication date
DK0453969T3 (en) 1997-02-10
NO911557D0 (en) 1991-04-19
EP0453969A1 (en) 1991-10-30
ATE142263T1 (en) 1996-09-15
ES2093043T3 (en) 1996-12-16
IE911322A1 (en) 1991-10-23
FI911882A (en) 1991-10-22
NO911557L (en) 1991-10-22
PL289953A1 (en) 1991-11-04
PT97427A (en) 1992-01-31
SK110191A3 (en) 1995-07-11
HU911302D0 (en) 1991-10-28
CZ285440B6 (en) 1999-08-11
CN1049248C (en) 2000-02-09
AU7515491A (en) 1991-10-24
HRP940770A2 (en) 1997-06-30
JP3319605B2 (en) 2002-09-03
CA2040810C (en) 2001-07-24
KR910018551A (en) 1991-11-30
TW213487B (en) 1993-09-21
EP0453969B1 (en) 1996-09-04
BR9101587A (en) 1991-12-10
HU210358B (en) 1995-04-28
DE59108128D1 (en) 1996-10-10
IL97903A0 (en) 1992-06-21
ZA912937B (en) 1991-12-24
CN1055952A (en) 1991-11-06
HUT57268A (en) 1991-11-28
RU2055892C1 (en) 1996-03-10
PL169178B1 (en) 1996-06-28
FI911882A0 (en) 1991-04-18
AU630287B2 (en) 1992-10-22
LV10494A (en) 1995-02-20
YU69691A (en) 1995-12-04
LT3686B (en) 1996-01-25
YU48435B (en) 1998-07-10
KR0168669B1 (en) 1999-01-15
NZ237882A (en) 1993-12-23
CZ110191A3 (en) 1993-08-11
PT97427B (en) 1998-08-31
DE4012818A1 (en) 1991-10-24
JPH04228086A (en) 1992-08-18
LTIP1523A (en) 1995-06-26
GR3021040T3 (en) 1996-12-31
LV10494B (en) 1996-02-20
PL169596B1 (en) 1996-08-30

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