KR101347951B1 - 핵산 증폭을 위한 프라이머, 프로브 및 방법 - Google Patents

핵산 증폭을 위한 프라이머, 프로브 및 방법 Download PDF

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KR101347951B1
KR101347951B1 KR1020137002325A KR20137002325A KR101347951B1 KR 101347951 B1 KR101347951 B1 KR 101347951B1 KR 1020137002325 A KR1020137002325 A KR 1020137002325A KR 20137002325 A KR20137002325 A KR 20137002325A KR 101347951 B1 KR101347951 B1 KR 101347951B1
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pcr
probe
late
primer
amplification
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로렌스 제이. 왕
존 라이스
아킬레스 제이. 산체스
케네쓰 피어스
제시 솔크
아더 레이스
크리스티나 하츠혼
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브랜데이스 유니버시티
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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KR1020137002325A 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법 Expired - Fee Related KR101347951B1 (ko)

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US61965404P 2004-10-18 2004-10-18
US60/619,654 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

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KR101347951B1 true KR101347951B1 (ko) 2014-01-07

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KR1020137025318A Expired - Fee Related KR101447405B1 (ko) 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법
KR1020077011244A Expired - Fee Related KR101424812B1 (ko) 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법

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KR1020077011244A Expired - Fee Related KR101424812B1 (ko) 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법

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US (3) US7632642B2 (https=)
EP (3) EP1805199B1 (https=)
JP (2) JP5543065B2 (https=)
KR (3) KR101347951B1 (https=)
CN (1) CN101076607B (https=)
AT (1) ATE494295T1 (https=)
AU (1) AU2005295298B2 (https=)
BR (1) BRPI0517105B1 (https=)
CA (3) CA2996947C (https=)
DE (1) DE602005025791D1 (https=)
DK (1) DK1805199T3 (https=)
ES (3) ES2668467T3 (https=)
NO (1) NO20072506L (https=)
PL (1) PL1805199T3 (https=)
PT (1) PT1805199E (https=)
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Families Citing this family (88)

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Publication number Priority date Publication date Assignee Title
WO2006044994A2 (en) 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
US7933011B2 (en) 2007-07-12 2011-04-26 Smiths Detection Inc. Fiber optic detection system
CA2740973A1 (en) * 2007-10-23 2009-04-30 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
EP2382324A1 (en) * 2008-12-30 2011-11-02 STMicroelectronics S.r.l. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムｐｃｒ装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US9034605B2 (en) 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
US8674080B2 (en) 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077841A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
AR077840A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
CN102471364B (zh) * 2009-08-14 2015-01-14 株式会社日立制作所 基因序列分析的方法和试剂
EP2491144B1 (en) 2009-10-21 2015-09-30 Brandeis University Methods for analyzing single-stranded nucleic acid sequences
US20110104693A1 (en) * 2009-11-03 2011-05-05 High Throughput Genomics, Inc. QUANTITATIVE NUCLEASE PROTECTION SEQUENCING (qNPS)
US20140186827A1 (en) * 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
WO2012024642A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Quantitative real-time pcr assay using fret dual-labeled primers
EP2614157A4 (en) * 2010-09-08 2014-03-26 Univ Brandeis Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
US20130295570A1 (en) * 2010-10-07 2013-11-07 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
ES2689257T3 (es) 2010-11-10 2018-11-12 Brandeis University Kit para la detección e identificación de micobacterias
US9637790B2 (en) 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA
US20140004504A1 (en) * 2010-12-10 2014-01-02 Brandeis University Compositions and methods for the detection and analysis of african swine fever virus
EP3181704B1 (en) 2010-12-27 2021-01-27 Abbott Molecular Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
CN103649335B (zh) 2011-05-04 2015-11-25 Htg分子诊断有限公司 定量核酸酶保护测定（qnpa）和测序（qnps）的改进
WO2013019759A1 (en) * 2011-07-30 2013-02-07 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
WO2013040491A2 (en) 2011-09-15 2013-03-21 Shafer David A Probe: antiprobe compositions for high specificity dna or rna detection
JP5570657B2 (ja) * 2011-10-31 2014-08-13 アークレイ株式会社 遺伝子存在量の測定方法
EP4361609A3 (en) 2012-02-03 2024-07-17 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
US9127317B2 (en) 2012-03-02 2015-09-08 Winthrop-University Hospital Method for using probe based PCR detection to measure the levels of circulating demethylated β cell derived DNA as a measure of β cell loss in diabetes
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
CN104662172B (zh) * 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
WO2014043660A2 (en) * 2012-09-17 2014-03-20 Brandeis University Combination of dsdna binding dye and probes for characterization of ssdna sequences
CN105339507A (zh) * 2013-02-21 2016-02-17 托马生物科学公司 用于核酸分析的方法、组合物和试剂盒
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
CN105745334B (zh) * 2013-09-30 2019-12-10 吴迪 通过使用邻近条形编码分析分子复合物的概况的方法
US10501783B2 (en) 2013-12-11 2019-12-10 Qiagen Gmbh Nucleic acid detection and quantification
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US20170247745A1 (en) * 2014-09-12 2017-08-31 Click Diagnostics, Inc. Multiplex optical detection
JP6835712B2 (ja) 2014-10-10 2021-02-24 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー 結核菌（Ｍｙｃｏｂａｃｔｅｒｉｕｍ Ｔｕｂｅｒｃｕｌｏｓｉｓ）のためのポリメラーゼ連鎖反応プライマーおよびプローブ
KR102084405B1 (ko) 2014-12-09 2020-03-04 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
US10240178B2 (en) 2014-12-19 2019-03-26 Brandeis University Mispriming prevention reagents
EP4029606A1 (en) 2014-12-31 2022-07-20 Visby Medical, Inc. Molecular diagnostic testing
EP3748002A1 (en) * 2015-01-12 2020-12-09 Life Technologies Corporation Methods, systems and compositions thereof for nucleic acid library quality control and quantification
EP3353697B1 (en) * 2015-09-24 2025-11-05 Seegene, Inc. Multiple dataset analysis for determining the presence or absence of target analyte
WO2017087943A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
CN109923613B (zh) * 2016-06-02 2023-07-04 Seegene株式会社 利用信号变化量数据集的样品内的目标分析物质检测方法
MX2018015889A (es) 2016-06-29 2019-05-27 Click Diagnostics Inc Dispositivos y metodos para la deteccion de moleculas usando una celda de flujo.
JP6983906B2 (ja) * 2017-04-11 2021-12-17 ニューゲン テクノロジーズ， インコーポレイテッド ライブラリーの定量および定性
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
KR102380264B1 (ko) * 2017-08-31 2022-03-29 주식회사 씨젠 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가
SG11202002931VA (en) 2017-11-09 2020-04-29 Visby Medical Inc Portable molecular diagnostic device and methods for the detection of target viruses
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅（武汉）生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
KR20200115552A (ko) 2018-01-22 2020-10-07 루미넥스 코포레이션 별개의 용융 분석을 위한 방법 및 조성물
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
AU2019255933B2 (en) * 2018-04-20 2022-08-25 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
KR20210081335A (ko) 2018-09-03 2021-07-01 비스비 메디컬, 인코포레이티드 항생제 감수성 시험을 위한 장치 및 방법
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
JP2022530323A (ja) 2019-04-28 2022-06-29 ビスビュー メディカル，インコーポレイテッド デジタル検出能力および無線接続性を有する分子診断デバイス
WO2021011850A2 (en) * 2019-07-17 2021-01-21 Chao Chien Chung Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
US20220340981A1 (en) 2019-09-23 2022-10-27 Universiteit Gent Probe and method for str-genotyping
US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术（深圳）有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
EP4388132A1 (en) * 2021-08-19 2024-06-26 Luminex Corporation Digital amplification assay analysis method
US12516389B2 (en) 2021-11-19 2026-01-06 Stmicroelectronics S.R.L. Microdot array having PCR-primers fixed in each microdot and method of forming the same on a substrate for gene based pathogen detection
CN119173642A (zh) * 2022-06-20 2024-12-20 株式会社日立高新技术 引物、dna检测方法和dna检测试剂盒
WO2026024677A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Method for determining the plasma volume of a blood sample and method for determining the dilution of plasma
WO2026024666A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Systems and methods for digital detection assays
WO2026024668A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Method of determining a volume of a liquid in a sample

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
EP1033411B1 (en) * 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
WO2001013086A2 (en) 1999-08-13 2001-02-22 Brandeis University Detection of nucleic acids
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
WO2001031062A1 (en) 1999-10-22 2001-05-03 The Public Health Research Institute Of The City Of New York, Inc. Assays for short sequence variants
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
EP1436391A4 (en) * 2001-06-08 2005-02-16 Shanghai Mendel Dna Ct Co Ltd CYCLIC EXTENSION AT LOW TEMPERATURE OF DNA WITH HIGH SPECIFICITY OF BEGINNING
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
CA2462505A1 (en) * 2001-10-25 2003-05-15 Gorilla Genomics, Inc. Asymmetric pcr with nuclease-free polymerase or nuclease-resistant molecular beacons
WO2003045233A1 (en) 2001-11-21 2003-06-05 Optiscan Biomedical Corporation Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
US7659060B2 (en) * 2002-09-02 2010-02-09 Toyo Boseki Kabushiki Kaisha Method for identifying nucleotide polymorphism
WO2004048516A2 (en) * 2002-11-22 2004-06-10 North Shore-Long Island Jewish Research Institute Elf3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
EP1709200B1 (en) * 2003-12-03 2012-05-09 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
CN101501211A (zh) * 2004-03-31 2009-08-05 综合医院公司 测定癌症对表皮生长因子受体靶向性治疗反应性的方法
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Proc. Natl. Acad. Sci. U. S. A., Vol. 101, No. 7, pp. 1933-1938 (2004.02.09.) *

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