KR100825147B1 - 신규의 니트릴히드라타제 - Google Patents

신규의 니트릴히드라타제 Download PDF

Info

Publication number
KR100825147B1
KR100825147B1 KR1020057011335A KR20057011335A KR100825147B1 KR 100825147 B1 KR100825147 B1 KR 100825147B1 KR 1020057011335 A KR1020057011335 A KR 1020057011335A KR 20057011335 A KR20057011335 A KR 20057011335A KR 100825147 B1 KR100825147 B1 KR 100825147B1
Authority
KR
South Korea
Prior art keywords
amino acid
substituted
subunit
nitrile hydratase
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
KR1020057011335A
Other languages
English (en)
Korean (ko)
Other versions
KR20050089972A (ko
Inventor
토시후미 야마키
신이치 반바
카오리 마토이시
키요시 이토
히데키 고바야시
에이시 타나카
토시히로 오이카와
Original Assignee
미쓰이 가가쿠 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 미쓰이 가가쿠 가부시키가이샤 filed Critical 미쓰이 가가쿠 가부시키가이샤
Publication of KR20050089972A publication Critical patent/KR20050089972A/ko
Application granted granted Critical
Publication of KR100825147B1 publication Critical patent/KR100825147B1/ko
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01084—Nitrile hydratase (4.2.1.84)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020057011335A 2002-12-19 2003-12-15 신규의 니트릴히드라타제 Expired - Lifetime KR100825147B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2002368360A JP2004194588A (ja) 2002-12-19 2002-12-19 新規なニトリルヒドラターゼ
JPJP-P-2002-00368360 2002-12-19
JP2003379280 2003-11-10
JPJP-P-2003-00379280 2003-11-10

Related Child Applications (4)

Application Number Title Priority Date Filing Date
KR1020067026865A Division KR100785549B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020077024119A Division KR100819228B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020077015994A Division KR100794001B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026866A Division KR100785548B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제

Publications (2)

Publication Number Publication Date
KR20050089972A KR20050089972A (ko) 2005-09-09
KR100825147B1 true KR100825147B1 (ko) 2008-04-24

Family

ID=32684188

Family Applications (5)

Application Number Title Priority Date Filing Date
KR1020057011335A Expired - Lifetime KR100825147B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020077015994A Expired - Lifetime KR100794001B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026865A Expired - Lifetime KR100785549B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026866A Expired - Lifetime KR100785548B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020077024119A Expired - Lifetime KR100819228B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제

Family Applications After (4)

Application Number Title Priority Date Filing Date
KR1020077015994A Expired - Lifetime KR100794001B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026865A Expired - Lifetime KR100785549B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026866A Expired - Lifetime KR100785548B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020077024119A Expired - Lifetime KR100819228B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제

Country Status (7)

Country Link
US (1) US7595184B2 (enExample)
EP (1) EP1586637B1 (enExample)
JP (1) JP2004194588A (enExample)
KR (5) KR100825147B1 (enExample)
CN (4) CN1729288A (enExample)
AU (2) AU2003289338A1 (enExample)
WO (1) WO2004056990A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4922757B2 (ja) * 2004-05-26 2012-04-25 三菱レイヨン株式会社 改良型ニトリルヒドラターゼ
EP1842907A1 (en) 2006-04-07 2007-10-10 B.R.A.I.N. Ag A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
JP5069032B2 (ja) * 2007-04-04 2012-11-07 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
CN105624139A (zh) * 2008-11-14 2016-06-01 三井化学株式会社 腈水合酶突变体
JP5532618B2 (ja) * 2009-01-30 2014-06-25 三菱レイヨン株式会社 改良型ニトリルヒドラターゼ及びその製造方法
KR20120058452A (ko) 2009-04-30 2012-06-07 미쓰이 가가쿠 가부시키가이샤 3-메르캅토프로피온산 또는 그 염을 제조하는 방법
AU2012264058B2 (en) 2011-05-31 2015-10-22 Mitsubishi Chemical Corporation Improved nitrile hydratase
EP2811021B1 (en) 2011-06-07 2016-11-02 Mitsubishi Rayon Co., Ltd. Improved nitrile hydratase
CN102517271B (zh) * 2011-12-13 2013-04-03 清华大学 一种突变腈水合酶
CN116536293A (zh) 2014-06-06 2023-08-04 三菱化学株式会社 改良型腈水合酶
CN104725476B (zh) * 2015-03-31 2018-10-16 清华大学 一种钩状结构寡肽及其应用
RU2736086C1 (ru) * 2016-12-28 2020-11-11 Мицуи Кемикалс, Инк. Мутантная нитрилгидратаза, нуклеиновая кислота, кодирующая указанную мутантную нитрилгидратазу, вектор экспрессии и трансформант, содержащие указанную нуклеиновую кислоту, способ получения указанной мутантной нитрилгидратазы и способ получения амидного соединения
KR20190049109A (ko) 2017-11-01 2019-05-09 (주)대화정공 풀리 제조방법
KR102146408B1 (ko) 2019-04-08 2020-08-21 (주)대화정공 단류선 개선을 위해 압연롤러를 이용한 자동차 댐퍼풀리 제조방법
KR102068615B1 (ko) 2019-04-08 2020-01-21 (주)대화정공 자동차 댐퍼풀리 제조방법
CN111690675B (zh) * 2019-08-01 2022-07-19 安徽瑞邦生物科技有限公司 一种表达腈水合酶突变体的重组菌及其制备方法和应用
CN112322606B (zh) * 2020-11-20 2022-05-10 江南大学 一种腈水合酶突变体及其应用
CN112522245A (zh) * 2020-12-09 2021-03-19 江南大学 一种腈水合酶氨基酸基序的改造及其应用
CN113151234B (zh) * 2021-04-13 2022-08-12 浙江工业大学 腈水合酶赖氨酸突变体hba-k2h2r、编码基因及应用
CN113444714B (zh) * 2021-07-29 2022-06-21 浙江大学杭州国际科创中心 一种β亚基突变的腈水合酶突变体及其应用
CN114317507B (zh) * 2021-11-30 2024-07-02 清华大学 腈水合酶突变体及其应用
CN119343452A (zh) * 2022-06-30 2025-01-21 三井化学株式会社 突变型腈水合酶、编码该突变型腈水合酶的核酸、含有该核酸的载体及转化体、该突变型腈水合酶的制造方法、及酰胺化合物的制造方法
CN115772514B (zh) * 2022-11-03 2025-03-04 无锡新晨宇生物工程有限公司 一种腈水合酶底物通道氨基酸基序的改造用于肉桂酰胺的制备
EP4600368A1 (en) * 2024-02-09 2025-08-13 Adisseo France S.A.S. A method for enzymatic production of an amide compound

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09275978A (ja) * 1996-02-14 1997-10-28 Mitsui Toatsu Chem Inc 新規なニトリルヒドラターゼ

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD274631A5 (de) 1987-09-18 1989-12-27 Kk Verfahren zur biologischen herstellung von amiden
AU627648B2 (en) 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JPH0856684A (ja) 1994-08-16 1996-03-05 Mitsui Toatsu Chem Inc 微生物によるアミド化合物の製造法
DE69739026D1 (de) 1996-02-14 2008-11-20 Mitsui Chemicals Inc Nitril-Hydratase, ihre Derivate und Verfahren zur Herstellung von Verbindungen
JP3408737B2 (ja) 1998-03-16 2003-05-19 三井化学株式会社 ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09275978A (ja) * 1996-02-14 1997-10-28 Mitsui Toatsu Chem Inc 新規なニトリルヒドラターゼ

Also Published As

Publication number Publication date
KR100785549B1 (ko) 2007-12-12
CN1729288A (zh) 2006-02-01
KR20070110146A (ko) 2007-11-15
AU2003289338A1 (en) 2004-07-14
KR100819228B1 (ko) 2008-04-03
EP1586637B1 (en) 2014-02-26
AU2010202683A1 (en) 2010-07-15
CN101892211A (zh) 2010-11-24
KR100785548B1 (ko) 2007-12-12
JP2004194588A (ja) 2004-07-15
KR20070087054A (ko) 2007-08-27
CN103215242A (zh) 2013-07-24
KR20070007393A (ko) 2007-01-15
EP1586637A1 (en) 2005-10-19
EP1586637A4 (en) 2008-03-19
KR20050089972A (ko) 2005-09-09
AU2010202683B2 (en) 2012-01-19
KR100794001B1 (ko) 2008-01-10
WO2004056990A1 (ja) 2004-07-08
US7595184B2 (en) 2009-09-29
CN103103175A (zh) 2013-05-15
KR20070011620A (ko) 2007-01-24
US20070009985A1 (en) 2007-01-11

Similar Documents

Publication Publication Date Title
KR100825147B1 (ko) 신규의 니트릴히드라타제
KR102324118B1 (ko) 변이형 나이트릴 히드라타아제, 그 변이형 나이트릴 히드라타아제를 코드하는 핵산, 그 핵산을 포함하는 발현 벡터 및 형질전환체, 그 변이형 나이트릴 히드라타아제의 제조 방법, 및 아마이드 화합물의 제조 방법
EP0774512A2 (en) A method for production of protein using molecular chaperon
CN111471669A (zh) 一种肝素裂解酶突变体及其重组表达的方法
JP3408737B2 (ja) ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子
US11859229B2 (en) Polyvinyl alcohol-degrading enzyme and process for producing the same
JP2005160403A (ja) ニトリルヒドラターゼ活性を有する酵素の改変方法
JP4216719B2 (ja) ハロゲン化合物耐性新規ギ酸脱水素酵素及びその製造方法
JPH08196281A (ja) 水生成型ｎａｄｈオキシダーゼをコードするｄｎａ
JPWO2002046427A1 (ja) 新規ギ酸脱水素酵素及びその製造方法
JPWO2002010399A1 (ja) 新規カルボニル還元酵素、その遺伝子、およびその利用法
KR100270508B1 (ko) 신규세파로스포린디아세틸라아제유전자및이를이용한단백질제조방법
CN110904076B (zh) 一种耐氯化钾的木糖苷酶突变体k317d及其应用
JP2005102511A (ja) 新規アルコール脱水素酵素、その遺伝子
WO2000031245A1 (en) Creatine amidinohydrolase and process for producing the same
JP4686090B2 (ja) マンノースイソメラーゼ遺伝子
JP2023141245A (ja) ３－ヒドロキシカルボン酸化合物又はその塩の製造方法
JP2003088384A (ja) 新規なニトリルヒドラターゼ
JP2009077722A (ja) 新規なニトリルヒドラターゼ
JP2007202432A (ja) 耐熱性カタラーゼ及びその製造方法
JPWO2001025443A1 (ja) マンノースイソメラーゼ遺伝子
JP2009017875A (ja) 新規カタラーゼ及びこれをコードする遺伝子
JPH11243962A (ja) 新規イヌリン分解酵素遺伝子及びその利用
JP2004261105A (ja) 新規なニトリルヒドラターゼ

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20050617

Patent event code: PA01051R01D

Comment text: International Patent Application

A201 Request for examination
AMND Amendment
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20050620

Comment text: Request for Examination of Application

PG1501 Laying open of application
AMND Amendment
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20060630

Patent event code: PE09021S01D

A107 Divisional application of patent
AMND Amendment
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20061220

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20070313

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20060630

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I

J201 Request for trial against refusal decision
PJ0201 Trial against decision of rejection

Patent event date: 20070612

Comment text: Request for Trial against Decision on Refusal

Patent event code: PJ02012R01D

Patent event date: 20070313

Comment text: Decision to Refuse Application

Patent event code: PJ02011S01I

Appeal kind category: Appeal against decision to decline refusal

Decision date: 20080131

Appeal identifier: 2007101006317

Request date: 20070612

A107 Divisional application of patent
AMND Amendment
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20070712

PB0901 Examination by re-examination before a trial

Comment text: Amendment to Specification, etc.

Patent event date: 20070712

Patent event code: PB09011R02I

Comment text: Request for Trial against Decision on Refusal

Patent event date: 20070612

Patent event code: PB09011R01I

Comment text: Amendment to Specification, etc.

Patent event date: 20061220

Patent event code: PB09011R02I

Comment text: Amendment to Specification, etc.

Patent event date: 20051118

Patent event code: PB09011R02I

Comment text: Amendment to Specification, etc.

Patent event date: 20050620

Patent event code: PB09011R02I

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20070821

Patent event code: PE09021S01D

A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20071019

B701 Decision to grant
PB0701 Decision of registration after re-examination before a trial

Patent event date: 20080131

Comment text: Decision to Grant Registration

Patent event code: PB07012S01D

Patent event date: 20070723

Comment text: Transfer of Trial File for Re-examination before a Trial

Patent event code: PB07011S01I

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20080418

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20080418

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20110318

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20120322

Start annual number: 5

End annual number: 5

FPAY Annual fee payment

Payment date: 20130321

Year of fee payment: 6

PR1001 Payment of annual fee

Payment date: 20130321

Start annual number: 6

End annual number: 6

FPAY Annual fee payment

Payment date: 20140411

Year of fee payment: 7

PR1001 Payment of annual fee

Payment date: 20140411

Start annual number: 7

End annual number: 7

FPAY Annual fee payment

Payment date: 20150416

Year of fee payment: 8

PR1001 Payment of annual fee

Payment date: 20150416

Start annual number: 8

End annual number: 8

FPAY Annual fee payment

Payment date: 20160408

Year of fee payment: 9

PR1001 Payment of annual fee

Payment date: 20160408

Start annual number: 9

End annual number: 9

FPAY Annual fee payment

Payment date: 20170407

Year of fee payment: 10

PR1001 Payment of annual fee

Payment date: 20170407

Start annual number: 10

End annual number: 10

FPAY Annual fee payment

Payment date: 20180410

Year of fee payment: 11

PR1001 Payment of annual fee

Payment date: 20180410

Start annual number: 11

End annual number: 11

PC1801 Expiration of term

Termination date: 20240615

Termination category: Expiration of duration