JP6276201B2 - 核酸の検出 - Google Patents

核酸の検出 Download PDF

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JP6276201B2
JP6276201B2 JP2014556886A JP2014556886A JP6276201B2 JP 6276201 B2 JP6276201 B2 JP 6276201B2 JP 2014556886 A JP2014556886 A JP 2014556886A JP 2014556886 A JP2014556886 A JP 2014556886A JP 6276201 B2 JP6276201 B2 JP 6276201B2
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primer
nucleotides
sequence
complementary
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JP2015511818A (ja
JP2015511818A5 (enExample
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アリソン ヴェルイヤン トッド
アリソン ヴェルイヤン トッド
エリーザ モカニー
エリーザ モカニー
サマンサ ウォーカー
サマンサ ウォーカー
コートニー ドナルド
コートニー ドナルド
ディーナ ロナーガン
ディーナ ロナーガン
タン リット イーン
タン リット イーン
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スピーデクス ピーティーワイ リミテッド
スピーデクス ピーティーワイ リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2014556886A 2012-02-20 2013-02-20 核酸の検出 Active JP6276201B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2012900624A AU2012900624A0 (en) 2012-02-20 Detection of Nucleic Acids
AU2012900624 2012-02-20
AU2012902218A AU2012902218A0 (en) 2012-05-29 Detection of nucleic acids
AU2012902218 2012-05-29
PCT/AU2013/000149 WO2013123552A1 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Related Child Applications (1)

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JP2018002618A Division JP6836302B2 (ja) 2012-02-20 2018-01-11 核酸の検出

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JP2015511818A JP2015511818A (ja) 2015-04-23
JP2015511818A5 JP2015511818A5 (enExample) 2016-04-14
JP6276201B2 true JP6276201B2 (ja) 2018-02-07

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JP2018002618A Active JP6836302B2 (ja) 2012-02-20 2018-01-11 核酸の検出

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US (3) US9963738B2 (enExample)
EP (1) EP2817421B1 (enExample)
JP (2) JP6276201B2 (enExample)
KR (1) KR102085119B1 (enExample)
CN (1) CN104245958B (enExample)
AU (1) AU2013202920B9 (enExample)
BR (1) BR112014020422B1 (enExample)
CA (1) CA2863808C (enExample)
DK (1) DK2817421T3 (enExample)
ES (1) ES2660248T3 (enExample)
IL (1) IL233958A (enExample)
MX (1) MX353840B (enExample)
NO (1) NO2817421T3 (enExample)
NZ (1) NZ628883A (enExample)
SG (1) SG11201405012YA (enExample)
WO (1) WO2013123552A1 (enExample)
ZA (1) ZA201406410B (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018075033A (ja) * 2012-02-20 2018-05-17 スピーデクス ピーティーワイ リミテッド 核酸の検出
CN112852934A (zh) * 2021-04-16 2021-05-28 武汉友芝友医疗科技股份有限公司 检测基因甲基化的引物、试剂和试剂盒

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AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
JP6544861B2 (ja) 2013-02-07 2019-07-17 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー 高い選択性の核酸増幅プライマー
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
ES2813825T3 (es) * 2014-04-25 2021-03-25 Dnae Group Holdings Ltd Métodos de secuenciación
KR101631114B1 (ko) * 2014-06-13 2016-06-16 서울대학교산학협력단 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법
US20170335382A1 (en) * 2014-11-10 2017-11-23 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Isothermal Amplification Assay for the Detection of Short Nucleic Acid Sequences
CN107614697A (zh) * 2015-02-26 2018-01-19 奥斯瑞根公司 用于提高突变评估准确性的方法和装置
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
EP3708677A1 (en) * 2015-07-17 2020-09-16 Luminex Corporation Methods and compositions for catalytic dna assays
CN114807323A (zh) * 2015-10-09 2022-07-29 安可济控股有限公司 用于富集扩增产物的方法及组合物
US20200299754A1 (en) 2017-04-11 2020-09-24 Speedx Pty Ltd Detection cascades
EP3665305A1 (en) 2017-08-10 2020-06-17 Katholieke Universiteit Leuven Nucleic acid enzyme sensor
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
KR101987388B1 (ko) * 2017-10-19 2019-06-12 경상대학교산학협력단 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도
CN107966438B (zh) * 2017-10-27 2020-11-24 中国农业大学 一种基于锌的功能核酸的耐高盐传感器及其应用
WO2019150414A1 (ja) * 2018-01-30 2019-08-08 学校法人 慶應義塾 核酸の検出方法
SG11202101140TA (en) * 2018-08-09 2021-03-30 Speedx Pty Ltd Multiplex detection of nucleic acids
CN109097468A (zh) * 2018-08-14 2018-12-28 南通市第人民医院 Opcml基因启动子甲基化水平的检测试剂盒及其应用
CN110452988A (zh) * 2019-08-27 2019-11-15 武汉友芝友医疗科技股份有限公司 检测esr1基因突变的引物组、试剂、试剂盒和方法
JP7744025B2 (ja) 2019-10-02 2025-09-25 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー 希少配列変異体を検出するためのアッセイ方法およびキット
CN116018414A (zh) * 2020-06-30 2023-04-25 斯皮德斯私人有限公司 使用混合报告子的多重核酸检测
KR20230098259A (ko) 2020-10-29 2023-07-03 비오까르띠 엔브이 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법
CN112662804B (zh) * 2021-01-25 2022-05-10 中国水稻研究所 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法
EP4288557A4 (en) * 2021-02-02 2024-12-25 RhoDx, Inc. SYNTHETIC POLYNUCLEOTIDES AND METHODS FOR SELECTIVE ALLELE AMPLIFICATION
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CN114609105B (zh) * 2022-03-11 2024-12-13 济南大学 一种联合检测miRNA155和谷胱甘肽的荧光生物传感器
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CN122235147A (zh) * 2026-05-20 2026-06-19 温州医科大学慈溪生物医药研究院 一种基于胆固醇纳米胶束自组装的多组分核酸酶系统及其在snp检测中的应用

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018075033A (ja) * 2012-02-20 2018-05-17 スピーデクス ピーティーワイ リミテッド 核酸の検出
CN112852934A (zh) * 2021-04-16 2021-05-28 武汉友芝友医疗科技股份有限公司 检测基因甲基化的引物、试剂和试剂盒

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EP2817421A4 (en) 2016-01-06
AU2013202920B2 (en) 2015-09-17
JP2018075033A (ja) 2018-05-17
WO2013123552A9 (en) 2014-08-28
US20190024160A1 (en) 2019-01-24
EP2817421B1 (en) 2017-12-13
JP2015511818A (ja) 2015-04-23
MX353840B (es) 2018-01-31
SG11201405012YA (en) 2014-09-26
NO2817421T3 (enExample) 2018-05-12
AU2013202920B9 (en) 2015-10-01
US20210108261A1 (en) 2021-04-15
BR112014020422A2 (pt) 2018-10-30
CA2863808C (en) 2020-07-21
HK1199743A1 (en) 2015-07-17
ES2660248T3 (es) 2018-03-21
US9963738B2 (en) 2018-05-08
BR112014020422B1 (pt) 2021-12-28
US11802308B2 (en) 2023-10-31
CN104245958B (zh) 2018-05-22
IL233958A (en) 2017-03-30
CN104245958A (zh) 2014-12-24
AU2013202920A1 (en) 2013-09-05
KR20140139497A (ko) 2014-12-05
IL233958A0 (en) 2014-09-30
US10724080B2 (en) 2020-07-28
ZA201406410B (en) 2015-09-30
EP2817421A1 (en) 2014-12-31
JP6836302B2 (ja) 2021-02-24
KR102085119B1 (ko) 2020-03-05
WO2013123552A1 (en) 2013-08-29
CA2863808A1 (en) 2013-08-29
DK2817421T3 (en) 2018-03-05
US20160348161A1 (en) 2016-12-01
MX2014010007A (es) 2014-09-12
AU2013202920A9 (en) 2015-08-20
NZ628883A (en) 2015-07-31

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