KR102085119B1 - 핵산의 검출 - Google Patents

핵산의 검출 Download PDF

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KR102085119B1
KR102085119B1 KR1020147025284A KR20147025284A KR102085119B1 KR 102085119 B1 KR102085119 B1 KR 102085119B1 KR 1020147025284 A KR1020147025284 A KR 1020147025284A KR 20147025284 A KR20147025284 A KR 20147025284A KR 102085119 B1 KR102085119 B1 KR 102085119B1
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primer
delete delete
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nucleotides
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KR20140139497A (ko
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앨리슨 벨이안 토드
엘리사 모카니
사만다 월커
코어트니 도널드
디나 로너건
탠 리트 인
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스피디엑스 피티와이 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020147025284A 2012-02-20 2013-02-20 핵산의 검출 Active KR102085119B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2012900624 2012-02-20
AU2012900624A AU2012900624A0 (en) 2012-02-20 Detection of Nucleic Acids
AU2012902218 2012-05-29
AU2012902218A AU2012902218A0 (en) 2012-05-29 Detection of nucleic acids
PCT/AU2013/000149 WO2013123552A1 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Publications (2)

Publication Number Publication Date
KR20140139497A KR20140139497A (ko) 2014-12-05
KR102085119B1 true KR102085119B1 (ko) 2020-03-05

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US (3) US9963738B2 (enExample)
EP (1) EP2817421B1 (enExample)
JP (2) JP6276201B2 (enExample)
KR (1) KR102085119B1 (enExample)
CN (1) CN104245958B (enExample)
AU (1) AU2013202920B9 (enExample)
BR (1) BR112014020422B1 (enExample)
CA (1) CA2863808C (enExample)
DK (1) DK2817421T3 (enExample)
ES (1) ES2660248T3 (enExample)
IL (1) IL233958A (enExample)
MX (1) MX353840B (enExample)
NO (1) NO2817421T3 (enExample)
NZ (1) NZ628883A (enExample)
SG (1) SG11201405012YA (enExample)
WO (1) WO2013123552A1 (enExample)
ZA (1) ZA201406410B (enExample)

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RU2650806C2 (ru) 2011-09-09 2018-04-17 Спидкс Пти Лтд Субстраты нуклеиновых кислот с ферментативной активностью
AU2013202920B9 (en) * 2012-02-20 2015-10-01 Speedx Pty Ltd Detection of nucleic acids
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
SG11201506146TA (en) * 2013-02-07 2015-09-29 Univ Rutgers Highly selective nucleic acid amplification primers
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
CN106460044B (zh) * 2014-04-25 2021-03-19 DNAe集团控股有限公司 测序方法
KR101631114B1 (ko) * 2014-06-13 2016-06-16 서울대학교산학협력단 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법
WO2016077291A1 (en) * 2014-11-10 2016-05-19 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Isothermal amplification assay for the detection of short nucleic acid sequences
AU2016222569A1 (en) * 2015-02-26 2017-09-07 Asuragen, Inc. Methods and apparatuses for improving mutation assessment accuracy
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
AU2016296594B2 (en) * 2015-07-17 2020-07-09 Luminex Corporation Methods and compositions for catalytic assays
CA3048046A1 (en) 2017-04-11 2018-10-18 Speedx Pty Ltd Catalytic enzyme detection cascades
US11614403B2 (en) 2017-08-10 2023-03-28 Fox Biosystems Nv Nucleic acid enzyme sensor
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
KR101987388B1 (ko) * 2017-10-19 2019-06-12 경상대학교산학협력단 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도
CN107966438B (zh) * 2017-10-27 2020-11-24 中国农业大学 一种基于锌的功能核酸的耐高盐传感器及其应用
WO2019150414A1 (ja) * 2018-01-30 2019-08-08 学校法人 慶應義塾 核酸の検出方法
KR102796204B1 (ko) * 2018-08-09 2025-04-14 스피디엑스 피티와이 리미티드 핵산의 다중 검출
CN109097468A (zh) * 2018-08-14 2018-12-28 南通市第人民医院 Opcml基因启动子甲基化水平的检测试剂盒及其应用
CN110452988A (zh) * 2019-08-27 2019-11-15 武汉友芝友医疗科技股份有限公司 检测esr1基因突变的引物组、试剂、试剂盒和方法
IL291819A (en) 2019-10-02 2022-06-01 Univ Rutgers Assay methods and kits for detecting rare sequence variants
US20230220463A1 (en) * 2020-06-30 2023-07-13 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters
JP2023547498A (ja) 2020-10-29 2023-11-10 ビオカルティ ナームローゼ フェノーツハップ マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法
CN112662804B (zh) * 2021-01-25 2022-05-10 中国水稻研究所 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法
KR20230141826A (ko) * 2021-02-02 2023-10-10 로디엑스, 인코포레이티드 합성 폴리뉴클레오티드 및 대립유전자를 선택적으로 증폭하기 위한 방법
CN112852934B (zh) * 2021-04-16 2022-02-18 武汉友芝友医疗科技股份有限公司 检测基因甲基化的引物、试剂和试剂盒
WO2023067110A1 (en) * 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
CN114609105B (zh) * 2022-03-11 2024-12-13 济南大学 一种联合检测miRNA155和谷胱甘肽的荧光生物传感器

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WO2011001496A1 (ja) 2009-06-29 2011-01-06 株式会社 東芝 検体解析方法およびそれに用いるアッセイキット

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WO2008122084A1 (en) 2007-04-05 2008-10-16 Johnson & Johnson Research Pty Limited Nucleic acid enzymes and complexes and methods for their use
WO2011001496A1 (ja) 2009-06-29 2011-01-06 株式会社 東芝 検体解析方法およびそれに用いるアッセイキット

Also Published As

Publication number Publication date
JP2015511818A (ja) 2015-04-23
CN104245958A (zh) 2014-12-24
US10724080B2 (en) 2020-07-28
EP2817421B1 (en) 2017-12-13
AU2013202920A1 (en) 2013-09-05
MX2014010007A (es) 2014-09-12
JP6836302B2 (ja) 2021-02-24
US9963738B2 (en) 2018-05-08
KR20140139497A (ko) 2014-12-05
CA2863808A1 (en) 2013-08-29
MX353840B (es) 2018-01-31
ZA201406410B (en) 2015-09-30
ES2660248T3 (es) 2018-03-21
AU2013202920B9 (en) 2015-10-01
BR112014020422A2 (pt) 2018-10-30
DK2817421T3 (en) 2018-03-05
JP2018075033A (ja) 2018-05-17
IL233958A (en) 2017-03-30
JP6276201B2 (ja) 2018-02-07
EP2817421A1 (en) 2014-12-31
AU2013202920B2 (en) 2015-09-17
CA2863808C (en) 2020-07-21
HK1199743A1 (en) 2015-07-17
US20190024160A1 (en) 2019-01-24
AU2013202920A9 (en) 2015-08-20
WO2013123552A9 (en) 2014-08-28
NO2817421T3 (enExample) 2018-05-12
CN104245958B (zh) 2018-05-22
NZ628883A (en) 2015-07-31
US11802308B2 (en) 2023-10-31
BR112014020422B1 (pt) 2021-12-28
SG11201405012YA (en) 2014-09-26
US20210108261A1 (en) 2021-04-15
IL233958A0 (en) 2014-09-30
WO2013123552A1 (en) 2013-08-29
US20160348161A1 (en) 2016-12-01
EP2817421A4 (en) 2016-01-06

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