AU2013202920B9 - Detection of nucleic acids - Google Patents

Detection of nucleic acids Download PDF

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Publication number
AU2013202920B9
AU2013202920B9 AU2013202920A AU2013202920A AU2013202920B9 AU 2013202920 B9 AU2013202920 B9 AU 2013202920B9 AU 2013202920 A AU2013202920 A AU 2013202920A AU 2013202920 A AU2013202920 A AU 2013202920A AU 2013202920 B9 AU2013202920 B9 AU 2013202920B9
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AU
Australia
Prior art keywords
component
nucleotides
primer
sequence
amplicon
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AU2013202920A
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AU2013202920A1 (en
AU2013202920B2 (en
AU2013202920A9 (en
Inventor
Cortny DONALD
Dina Lonergan
Elisa Mokany
Alison Velyian Todd
Samantha WALKER
Lit YEEN TAN
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SpeeDx Pty Ltd
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SpeeDx Pty Ltd
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Priority claimed from AU2012900624A external-priority patent/AU2012900624A0/en
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Priority to AU2013202920A priority Critical patent/AU2013202920B9/en
Publication of AU2013202920A1 publication Critical patent/AU2013202920A1/en
Publication of AU2013202920A9 publication Critical patent/AU2013202920A9/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/12Type of nucleic acid catalytic nucleic acids, e.g. ribozymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2013202920A 2012-02-20 2013-02-20 Detection of nucleic acids Active AU2013202920B9 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2013202920A AU2013202920B9 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
AU2012900624 2012-02-20
AU2012900624A AU2012900624A0 (en) 2012-02-20 Detection of Nucleic Acids
AU2012902218 2012-05-29
AU2012902218A AU2012902218A0 (en) 2012-05-29 Detection of nucleic acids
AU2013202920A AU2013202920B9 (en) 2012-02-20 2013-02-20 Detection of nucleic acids
PCT/AU2013/000149 WO2013123552A1 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Publications (4)

Publication Number Publication Date
AU2013202920A1 AU2013202920A1 (en) 2013-09-05
AU2013202920A9 AU2013202920A9 (en) 2015-08-20
AU2013202920B2 AU2013202920B2 (en) 2015-09-17
AU2013202920B9 true AU2013202920B9 (en) 2015-10-01

Family

ID=49004851

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013202920A Active AU2013202920B9 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Country Status (17)

Country Link
US (3) US9963738B2 (enExample)
EP (1) EP2817421B1 (enExample)
JP (2) JP6276201B2 (enExample)
KR (1) KR102085119B1 (enExample)
CN (1) CN104245958B (enExample)
AU (1) AU2013202920B9 (enExample)
BR (1) BR112014020422B1 (enExample)
CA (1) CA2863808C (enExample)
DK (1) DK2817421T3 (enExample)
ES (1) ES2660248T3 (enExample)
IL (1) IL233958A (enExample)
MX (1) MX353840B (enExample)
NO (1) NO2817421T3 (enExample)
NZ (1) NZ628883A (enExample)
SG (1) SG11201405012YA (enExample)
WO (1) WO2013123552A1 (enExample)
ZA (1) ZA201406410B (enExample)

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SG11201506146TA (en) * 2013-02-07 2015-09-29 Univ Rutgers Highly selective nucleic acid amplification primers
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
CN106460044B (zh) * 2014-04-25 2021-03-19 DNAe集团控股有限公司 测序方法
KR101631114B1 (ko) * 2014-06-13 2016-06-16 서울대학교산학협력단 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법
WO2016077291A1 (en) * 2014-11-10 2016-05-19 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Isothermal amplification assay for the detection of short nucleic acid sequences
AU2016222569A1 (en) * 2015-02-26 2017-09-07 Asuragen, Inc. Methods and apparatuses for improving mutation assessment accuracy
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
AU2016296594B2 (en) * 2015-07-17 2020-07-09 Luminex Corporation Methods and compositions for catalytic assays
CA3048046A1 (en) 2017-04-11 2018-10-18 Speedx Pty Ltd Catalytic enzyme detection cascades
US11614403B2 (en) 2017-08-10 2023-03-28 Fox Biosystems Nv Nucleic acid enzyme sensor
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
KR101987388B1 (ko) * 2017-10-19 2019-06-12 경상대학교산학협력단 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도
CN107966438B (zh) * 2017-10-27 2020-11-24 中国农业大学 一种基于锌的功能核酸的耐高盐传感器及其应用
WO2019150414A1 (ja) * 2018-01-30 2019-08-08 学校法人 慶應義塾 核酸の検出方法
KR102796204B1 (ko) * 2018-08-09 2025-04-14 스피디엑스 피티와이 리미티드 핵산의 다중 검출
CN109097468A (zh) * 2018-08-14 2018-12-28 南通市第人民医院 Opcml基因启动子甲基化水平的检测试剂盒及其应用
CN110452988A (zh) * 2019-08-27 2019-11-15 武汉友芝友医疗科技股份有限公司 检测esr1基因突变的引物组、试剂、试剂盒和方法
IL291819A (en) 2019-10-02 2022-06-01 Univ Rutgers Assay methods and kits for detecting rare sequence variants
US20230220463A1 (en) * 2020-06-30 2023-07-13 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters
JP2023547498A (ja) 2020-10-29 2023-11-10 ビオカルティ ナームローゼ フェノーツハップ マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法
CN112662804B (zh) * 2021-01-25 2022-05-10 中国水稻研究所 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法
KR20230141826A (ko) * 2021-02-02 2023-10-10 로디엑스, 인코포레이티드 합성 폴리뉴클레오티드 및 대립유전자를 선택적으로 증폭하기 위한 방법
CN112852934B (zh) * 2021-04-16 2022-02-18 武汉友芝友医疗科技股份有限公司 检测基因甲基化的引物、试剂和试剂盒
WO2023067110A1 (en) * 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
CN114609105B (zh) * 2022-03-11 2024-12-13 济南大学 一种联合检测miRNA155和谷胱甘肽的荧光生物传感器

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Also Published As

Publication number Publication date
JP2015511818A (ja) 2015-04-23
CN104245958A (zh) 2014-12-24
US10724080B2 (en) 2020-07-28
EP2817421B1 (en) 2017-12-13
AU2013202920A1 (en) 2013-09-05
MX2014010007A (es) 2014-09-12
JP6836302B2 (ja) 2021-02-24
US9963738B2 (en) 2018-05-08
KR20140139497A (ko) 2014-12-05
CA2863808A1 (en) 2013-08-29
MX353840B (es) 2018-01-31
ZA201406410B (en) 2015-09-30
ES2660248T3 (es) 2018-03-21
BR112014020422A2 (pt) 2018-10-30
DK2817421T3 (en) 2018-03-05
JP2018075033A (ja) 2018-05-17
IL233958A (en) 2017-03-30
JP6276201B2 (ja) 2018-02-07
EP2817421A1 (en) 2014-12-31
AU2013202920B2 (en) 2015-09-17
CA2863808C (en) 2020-07-21
HK1199743A1 (en) 2015-07-17
US20190024160A1 (en) 2019-01-24
AU2013202920A9 (en) 2015-08-20
WO2013123552A9 (en) 2014-08-28
NO2817421T3 (enExample) 2018-05-12
CN104245958B (zh) 2018-05-22
NZ628883A (en) 2015-07-31
US11802308B2 (en) 2023-10-31
BR112014020422B1 (pt) 2021-12-28
SG11201405012YA (en) 2014-09-26
US20210108261A1 (en) 2021-04-15
IL233958A0 (en) 2014-09-30
WO2013123552A1 (en) 2013-08-29
US20160348161A1 (en) 2016-12-01
KR102085119B1 (ko) 2020-03-05
EP2817421A4 (en) 2016-01-06

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