DK2817421T3 - DETECTION OF NUCLEIC ACIDS - Google Patents
DETECTION OF NUCLEIC ACIDS Download PDFInfo
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- DK2817421T3 DK2817421T3 DK13751988.0T DK13751988T DK2817421T3 DK 2817421 T3 DK2817421 T3 DK 2817421T3 DK 13751988 T DK13751988 T DK 13751988T DK 2817421 T3 DK2817421 T3 DK 2817421T3
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
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- Chemical & Material Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
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- Immunology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Applications Claiming Priority (3)
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| AU2012900624A AU2012900624A0 (en) | 2012-02-20 | Detection of Nucleic Acids | |
| AU2012902218A AU2012902218A0 (en) | 2012-05-29 | Detection of nucleic acids | |
| PCT/AU2013/000149 WO2013123552A1 (en) | 2012-02-20 | 2013-02-20 | Detection of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2817421T3 true DK2817421T3 (en) | 2018-03-05 |
Family
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Family Applications (1)
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| DK13751988.0T DK2817421T3 (en) | 2012-02-20 | 2013-02-20 | DETECTION OF NUCLEIC ACIDS |
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| EP (1) | EP2817421B1 (enExample) |
| JP (2) | JP6276201B2 (enExample) |
| KR (1) | KR102085119B1 (enExample) |
| CN (1) | CN104245958B (enExample) |
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| BR (1) | BR112014020422B1 (enExample) |
| CA (1) | CA2863808C (enExample) |
| DK (1) | DK2817421T3 (enExample) |
| ES (1) | ES2660248T3 (enExample) |
| IL (1) | IL233958A (enExample) |
| MX (1) | MX353840B (enExample) |
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| NZ (1) | NZ628883A (enExample) |
| SG (1) | SG11201405012YA (enExample) |
| WO (1) | WO2013123552A1 (enExample) |
| ZA (1) | ZA201406410B (enExample) |
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| CA2847697C (en) * | 2011-09-09 | 2020-04-21 | Speedx Pty Ltd | Nucleic acid enzyme substrates |
| KR102085119B1 (ko) | 2012-02-20 | 2020-03-05 | 스피디엑스 피티와이 리미티드 | 핵산의 검출 |
| AU2013202354A1 (en) * | 2012-06-18 | 2014-01-16 | Speedx Pty Ltd | Target detection and signal amplification |
| KR102222546B1 (ko) * | 2013-02-07 | 2021-03-08 | 러트거즈,더스테이트유니버시티오브뉴저지 | 고도로 선택적인 핵산 증폭 프라이머 |
| CN103540660B (zh) * | 2013-10-10 | 2015-10-28 | 中国人民解放军疾病预防控制所 | 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒 |
| EP3134542B1 (en) * | 2014-04-25 | 2020-06-03 | Dnae Group Holdings Limited | Sequencing methods |
| KR101631114B1 (ko) * | 2014-06-13 | 2016-06-16 | 서울대학교산학협력단 | 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법 |
| WO2016077291A1 (en) * | 2014-11-10 | 2016-05-19 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Isothermal amplification assay for the detection of short nucleic acid sequences |
| WO2016138376A1 (en) * | 2015-02-26 | 2016-09-01 | Asuragen, Inc. | Methods and apparatuses for improving mutation assessment accuracy |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| WO2017015177A1 (en) * | 2015-07-17 | 2017-01-26 | Luminex Corporation | Methods and compositions for catalytic assays |
| JP7486951B2 (ja) | 2017-04-11 | 2024-05-20 | スピーディックス プロプライアタリー リミティド | 検出カスケード |
| WO2019030383A1 (en) | 2017-08-10 | 2019-02-14 | Katholieke Universiteit Leuven | ENZYME SENSOR OF NUCLEIC ACIDS |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| KR101987388B1 (ko) * | 2017-10-19 | 2019-06-12 | 경상대학교산학협력단 | 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도 |
| CN107966438B (zh) * | 2017-10-27 | 2020-11-24 | 中国农业大学 | 一种基于锌的功能核酸的耐高盐传感器及其应用 |
| WO2019150414A1 (ja) * | 2018-01-30 | 2019-08-08 | 学校法人 慶應義塾 | 核酸の検出方法 |
| WO2020031156A1 (en) * | 2018-08-09 | 2020-02-13 | Speedx Pty Ltd | Multiplex detection of nucleic acids |
| CN109097468A (zh) * | 2018-08-14 | 2018-12-28 | 南通市第人民医院 | Opcml基因启动子甲基化水平的检测试剂盒及其应用 |
| CN110452988A (zh) * | 2019-08-27 | 2019-11-15 | 武汉友芝友医疗科技股份有限公司 | 检测esr1基因突变的引物组、试剂、试剂盒和方法 |
| CN114555829A (zh) | 2019-10-02 | 2022-05-27 | 新泽西鲁特格斯州立大学 | 检测稀有序列变体的测定方法和试剂盒 |
| AU2020256848A1 (en) * | 2020-06-30 | 2022-10-27 | Speedx Pty Ltd | Multiplex detection of nucleic acids using mixed reporters |
| KR20230098259A (ko) | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
| CN112662804B (zh) * | 2021-01-25 | 2022-05-10 | 中国水稻研究所 | 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法 |
| CA3206960A1 (en) * | 2021-02-02 | 2022-08-11 | Rhodx, Inc. | Synthetic polynucleotides and methods for selectively amplifying alleles |
| CN112852934B (zh) * | 2021-04-16 | 2022-02-18 | 武汉友芝友医疗科技股份有限公司 | 检测基因甲基化的引物、试剂和试剂盒 |
| DE112022005006T5 (de) * | 2021-10-20 | 2024-08-08 | Tataa Biocenter Ab | Verfahren und zusammensetzungen zur detektion mutierter nukleinsäuresequenzen |
| CN114609105B (zh) * | 2022-03-11 | 2024-12-13 | 济南大学 | 一种联合检测miRNA155和谷胱甘肽的荧光生物传感器 |
Family Cites Families (11)
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| US20040121374A1 (en) * | 2002-10-02 | 2004-06-24 | Yoshihide Iwaki | Method for detecting single nucleotide polymorphisms |
| KR101243266B1 (ko) * | 2005-03-05 | 2013-03-25 | 주식회사 씨젠 | 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드 |
| DK1948822T3 (da) * | 2005-10-07 | 2011-10-17 | Johnson & Johnson Res Pty Ltd | Multikomponent-nukleinsyreenzymer og fremgangsmåder til deres anvendelse |
| BRPI0718137B1 (pt) * | 2006-10-06 | 2016-05-10 | Johnson & Johnson Res Pty Ltd | comutadores moleculares e métodos para seu uso |
| CN101802216B (zh) * | 2007-04-05 | 2014-12-10 | 斯比戴克斯私人有限公司 | 核酸酶和复合体及其使用方法 |
| JP2010035532A (ja) * | 2008-08-08 | 2010-02-18 | Canon Inc | アレル特異的pcr法 |
| KR101378214B1 (ko) * | 2009-06-29 | 2014-03-27 | 가부시끼가이샤 도시바 | 검체 해석 방법 및 그것에 사용하는 분석 키트 |
| JP5115825B2 (ja) * | 2009-11-06 | 2013-01-09 | 独立行政法人農業・食品産業技術総合研究機構 | イグサ品種「ひのみどり」の識別マーカー |
| BR112012018394B8 (pt) * | 2009-12-21 | 2021-07-27 | Seegene Inc | método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo |
| JP5684737B2 (ja) * | 2010-01-21 | 2015-03-18 | アークレイ株式会社 | 標的配列の増幅方法、多型検出方法およびそれに用いる試薬 |
| KR102085119B1 (ko) | 2012-02-20 | 2020-03-05 | 스피디엑스 피티와이 리미티드 | 핵산의 검출 |
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2013
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- 2013-02-20 DK DK13751988.0T patent/DK2817421T3/en active
- 2013-02-20 BR BR112014020422-5A patent/BR112014020422B1/pt not_active IP Right Cessation
- 2013-02-20 ES ES13751988.0T patent/ES2660248T3/es active Active
- 2013-02-20 JP JP2014556886A patent/JP6276201B2/ja active Active
- 2013-02-20 CN CN201380019973.3A patent/CN104245958B/zh active Active
- 2013-02-20 SG SG11201405012YA patent/SG11201405012YA/en unknown
- 2013-02-20 NO NO13751988A patent/NO2817421T3/no unknown
- 2013-02-20 WO PCT/AU2013/000149 patent/WO2013123552A1/en not_active Ceased
- 2013-02-20 US US14/379,178 patent/US9963738B2/en active Active
- 2013-02-20 NZ NZ628883A patent/NZ628883A/en unknown
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2014
- 2014-08-05 IL IL233958A patent/IL233958A/en active IP Right Grant
- 2014-09-01 ZA ZA2014/06410A patent/ZA201406410B/en unknown
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- 2018-03-27 US US15/937,657 patent/US10724080B2/en active Active
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2020
- 2020-06-25 US US16/912,622 patent/US11802308B2/en active Active
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