BR112014020422B1 - Método para determinar a presença ou ausência de um polinucleotídeo alvo em uma amostra - Google Patents

Método para determinar a presença ou ausência de um polinucleotídeo alvo em uma amostra Download PDF

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BR112014020422B1
BR112014020422B1 BR112014020422-5A BR112014020422A BR112014020422B1 BR 112014020422 B1 BR112014020422 B1 BR 112014020422B1 BR 112014020422 A BR112014020422 A BR 112014020422A BR 112014020422 B1 BR112014020422 B1 BR 112014020422B1
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component
primer
amplicon
sequence
nucleotides
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BR112014020422-5A
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English (en)
Portuguese (pt)
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BR112014020422A2 (pt
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Alison Velyian Todd
Elisa Mokany
Samantha Walker
Cortny Donald
Dina Lonergan
Lit Yeen Tan
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Speedx Pty Ltd
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Priority claimed from AU2012900624A external-priority patent/AU2012900624A0/en
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Publication of BR112014020422A2 publication Critical patent/BR112014020422A2/pt
Publication of BR112014020422B1 publication Critical patent/BR112014020422B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/12Type of nucleic acid catalytic nucleic acids, e.g. ribozymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
BR112014020422-5A 2012-02-20 2013-02-20 Método para determinar a presença ou ausência de um polinucleotídeo alvo em uma amostra BR112014020422B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2012900624 2012-02-20
AU2012900624A AU2012900624A0 (en) 2012-02-20 Detection of Nucleic Acids
AU2012902218 2012-05-29
AU2012902218A AU2012902218A0 (en) 2012-05-29 Detection of nucleic acids
PCT/AU2013/000149 WO2013123552A1 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Publications (2)

Publication Number Publication Date
BR112014020422A2 BR112014020422A2 (pt) 2018-10-30
BR112014020422B1 true BR112014020422B1 (pt) 2021-12-28

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Country Link
US (3) US9963738B2 (enExample)
EP (1) EP2817421B1 (enExample)
JP (2) JP6276201B2 (enExample)
KR (1) KR102085119B1 (enExample)
CN (1) CN104245958B (enExample)
AU (1) AU2013202920B9 (enExample)
BR (1) BR112014020422B1 (enExample)
CA (1) CA2863808C (enExample)
DK (1) DK2817421T3 (enExample)
ES (1) ES2660248T3 (enExample)
IL (1) IL233958A (enExample)
MX (1) MX353840B (enExample)
NO (1) NO2817421T3 (enExample)
NZ (1) NZ628883A (enExample)
SG (1) SG11201405012YA (enExample)
WO (1) WO2013123552A1 (enExample)
ZA (1) ZA201406410B (enExample)

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CN106460044B (zh) * 2014-04-25 2021-03-19 DNAe集团控股有限公司 测序方法
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WO2016077291A1 (en) * 2014-11-10 2016-05-19 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Isothermal amplification assay for the detection of short nucleic acid sequences
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WO2019150414A1 (ja) * 2018-01-30 2019-08-08 学校法人 慶應義塾 核酸の検出方法
KR102796204B1 (ko) * 2018-08-09 2025-04-14 스피디엑스 피티와이 리미티드 핵산의 다중 검출
CN109097468A (zh) * 2018-08-14 2018-12-28 南通市第人民医院 Opcml基因启动子甲基化水平的检测试剂盒及其应用
CN110452988A (zh) * 2019-08-27 2019-11-15 武汉友芝友医疗科技股份有限公司 检测esr1基因突变的引物组、试剂、试剂盒和方法
IL291819A (en) 2019-10-02 2022-06-01 Univ Rutgers Assay methods and kits for detecting rare sequence variants
US20230220463A1 (en) * 2020-06-30 2023-07-13 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters
JP2023547498A (ja) 2020-10-29 2023-11-10 ビオカルティ ナームローゼ フェノーツハップ マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法
CN112662804B (zh) * 2021-01-25 2022-05-10 中国水稻研究所 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法
KR20230141826A (ko) * 2021-02-02 2023-10-10 로디엑스, 인코포레이티드 합성 폴리뉴클레오티드 및 대립유전자를 선택적으로 증폭하기 위한 방법
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Publication number Publication date
JP2015511818A (ja) 2015-04-23
CN104245958A (zh) 2014-12-24
US10724080B2 (en) 2020-07-28
EP2817421B1 (en) 2017-12-13
AU2013202920A1 (en) 2013-09-05
MX2014010007A (es) 2014-09-12
JP6836302B2 (ja) 2021-02-24
US9963738B2 (en) 2018-05-08
KR20140139497A (ko) 2014-12-05
CA2863808A1 (en) 2013-08-29
MX353840B (es) 2018-01-31
ZA201406410B (en) 2015-09-30
ES2660248T3 (es) 2018-03-21
AU2013202920B9 (en) 2015-10-01
BR112014020422A2 (pt) 2018-10-30
DK2817421T3 (en) 2018-03-05
JP2018075033A (ja) 2018-05-17
IL233958A (en) 2017-03-30
JP6276201B2 (ja) 2018-02-07
EP2817421A1 (en) 2014-12-31
AU2013202920B2 (en) 2015-09-17
CA2863808C (en) 2020-07-21
HK1199743A1 (en) 2015-07-17
US20190024160A1 (en) 2019-01-24
AU2013202920A9 (en) 2015-08-20
WO2013123552A9 (en) 2014-08-28
NO2817421T3 (enExample) 2018-05-12
CN104245958B (zh) 2018-05-22
NZ628883A (en) 2015-07-31
US11802308B2 (en) 2023-10-31
SG11201405012YA (en) 2014-09-26
US20210108261A1 (en) 2021-04-15
IL233958A0 (en) 2014-09-30
WO2013123552A1 (en) 2013-08-29
US20160348161A1 (en) 2016-12-01
KR102085119B1 (ko) 2020-03-05
EP2817421A4 (en) 2016-01-06

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