JP5779239B2 - 単一ｂ細胞培養法 - Google Patents

単一ｂ細胞培養法 Download PDF

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JP5779239B2
JP5779239B2 JP2013510646A JP2013510646A JP5779239B2 JP 5779239 B2 JP5779239 B2 JP 5779239B2 JP 2013510646 A JP2013510646 A JP 2013510646A JP 2013510646 A JP2013510646 A JP 2013510646A JP 5779239 B2 JP5779239 B2 JP 5779239B2
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cells
cell
igg
antibody
culture
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JP2013526286A (ja
JP2013526286A5 (enExample
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ジョセフ エンドル
ジョセフ エンドル
ナタリー シューマッハ
ナタリー シューマッハ
ソニア オフナー
ソニア オフナー
ジョセフ プラッツァー
ジョセフ プラッツァー
バシレ シーウェ
バシレ シーウェ
イルムガルト トレイ
イルムガルト トレイ
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2013510646A 2010-05-28 2011-05-26 単一ｂ細胞培養法 Active JP5779239B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10005602.7 2010-05-28
EP10005602.7A EP2400298B1 (en) 2010-05-28 2010-05-28 Single B-cell cultivation method and specific antibody production
PCT/EP2011/058616 WO2011147903A1 (en) 2010-05-28 2011-05-26 Single b-cell cultivation method

Related Child Applications (1)

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JP2015138308A Division JP6204415B2 (ja) 2010-05-28 2015-07-10 単一ｂ細胞培養法

Publications (3)

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JP2013526286A JP2013526286A (ja) 2013-06-24
JP2013526286A5 JP2013526286A5 (enExample) 2014-12-11
JP5779239B2 true JP5779239B2 (ja) 2015-09-16

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JP2013510646A Active JP5779239B2 (ja) 2010-05-28 2011-05-26 単一ｂ細胞培養法
JP2015138308A Active JP6204415B2 (ja) 2010-05-28 2015-07-10 単一ｂ細胞培養法
JP2017097126A Active JP6353953B2 (ja) 2010-05-28 2017-05-16 単一ｂ細胞培養法

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JP2017097126A Active JP6353953B2 (ja) 2010-05-28 2017-05-16 単一ｂ細胞培養法

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Country Link
US (4) US20130084637A1 (enExample)
EP (4) EP2400298B1 (enExample)
JP (3) JP5779239B2 (enExample)
KR (1) KR101495976B1 (enExample)
CN (2) CN102918395B (enExample)
BR (1) BR112012025695B1 (enExample)
CA (2) CA2798286C (enExample)
DK (1) DK2400298T3 (enExample)
ES (4) ES2434256T3 (enExample)
MX (2) MX345884B (enExample)
PL (3) PL2400298T3 (enExample)
RU (1) RU2709531C2 (enExample)
SI (1) SI2400298T1 (enExample)
WO (1) WO2011147903A1 (enExample)

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EP2914629A1 (en) 2012-11-05 2015-09-09 MAB Discovery GmbH Method for the production of multispecific antibodies
EP2727943A1 (en) 2012-11-05 2014-05-07 MAB Discovery GmbH Trispecific antibodies against human EGFR, HER2 and HER3
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AU2014232225B2 (en) * 2013-03-15 2020-03-19 H. Lundbeck A/S. Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens
CN104232577B (zh) * 2013-06-20 2017-12-01 中国人民解放军军事医学科学院基础医学研究所 一种获得自身反应性b细胞的方法
EP4141108A1 (en) * 2013-12-24 2023-03-01 Kling Biotherapeutics B.V. Ex vivo antibody production
JP7114460B2 (ja) 2015-06-26 2022-08-08 サノフィ・バイオテクノロジー モノクローナル抗ＩＬ－１ＲＡｃＰ抗体
EP3378488A4 (en) 2015-11-18 2019-10-30 Chugai Seiyaku Kabushiki Kaisha METHOD FOR IMPROVING THE HUMORAL IMMUNE REACTION
EP4273232A3 (en) 2016-03-30 2024-01-03 F. Hoffmann-La Roche AG B-cell cultivation method
EP3241845A1 (en) 2016-05-06 2017-11-08 MAB Discovery GmbH Humanized anti-il-1r3 antibodies
CN106222137A (zh) * 2016-08-24 2016-12-14 南昌大学 一种体外活化人记忆性b细胞成浆细胞的培养方法
WO2018112407A1 (en) 2016-12-15 2018-06-21 Duke University Antibodies and methods for depleting regulatory b10 cells and use in combination with immune checkpoint inhibitors
EP3562936B1 (en) * 2017-01-02 2024-05-22 F. Hoffmann-La Roche AG B-cell cultivation method
EP3401332A1 (en) 2017-05-08 2018-11-14 MAB Discovery GmbH Anti-il-1r3 antibodies for use in inflammatory conditions
JP6978516B2 (ja) 2017-05-19 2021-12-08 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 胸腺細胞上清を産生するための方法
US12009521B2 (en) * 2017-11-08 2024-06-11 Sharp Kabushiki Kaisha Negative electrode for batteries, battery, and method for producing battery
JP7166342B2 (ja) 2017-11-30 2022-11-07 エフ．ホフマン－ラ ロシュ アーゲー Ｂ細胞培養法
CN108588019B (zh) * 2018-05-03 2019-04-02 首都医科大学附属北京朝阳医院 一种体外诱导初始b细胞分化为调节性b细胞的方法及其培养条件
CN109234232A (zh) * 2018-09-30 2019-01-18 杭州华安单抗生物技术有限公司 兔外周血b细胞的培养体系及培养方法、抗体的制备方法和应用
JP7650802B2 (ja) 2018-12-30 2025-03-25 エフ． ホフマン－ラ ロシュ アーゲー 抗ウサギｃｄ１９抗体および使用方法
WO2021211997A1 (en) * 2020-04-16 2021-10-21 The General Hospital Corporation B cell immunomodulatory therapy for acute respiratory distress syndrome
CN111518765B (zh) * 2020-05-12 2021-01-26 优睿赛思(武汉)生物科技有限公司 一种b淋巴细胞体外培养体系及应用
AR130550A1 (es) 2022-09-21 2024-12-18 Sanofi Biotechnology Anticuerpo anti-il-1r3 humanizado y métodos de uso
CN120917139A (zh) 2023-04-05 2025-11-07 豪夫迈·罗氏有限公司 用于抗体表达细胞的体外培养方法

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