MX345884B - Metodo de cultivo de celulas b individuales. - Google Patents

Metodo de cultivo de celulas b individuales.

Info

Publication number
MX345884B
MX345884B MX2015001467A MX2015001467A MX345884B MX 345884 B MX345884 B MX 345884B MX 2015001467 A MX2015001467 A MX 2015001467A MX 2015001467 A MX2015001467 A MX 2015001467A MX 345884 B MX345884 B MX 345884B
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MX
Mexico
Prior art keywords
cells
interleukin
igg
cell
labeling
Prior art date
Application number
MX2015001467A
Other languages
English (en)
Inventor
Offner Sonja
Endl Josef
Platzer Josef
Schuhmacher Natalie
Siewe Basile
Thorey Irmgard
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX345884B publication Critical patent/MX345884B/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
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    • G01N33/531—Production of immunochemical test materials
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2502/00—Coculture with; Conditioned medium produced by
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
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    • G01N15/10—Investigating individual particles
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    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells

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  • Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invención se refiere a un método para seleccionar una célula B, caracterizado porque comprende las siguientes etapas: a) co-cultivar cada una de las células B, que se ha depositado como célula individual, con células EL-4 B5 de murino como células alimentadoras, b) seleccionar un clon de células B que prolifere y secrete anticuerpos en la etapa a), en donde el co-cultivo es en presencia de una mezcla alimentadora sintética que comprende IL-1ß, TNFa, IL-10, y uno o más seleccionados de IL-21, SAC, BAFF, IL-2 IL4 e IL-6.
MX2015001467A 2010-05-28 2011-05-26 Metodo de cultivo de celulas b individuales. MX345884B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10005602.7A EP2400298B1 (en) 2010-05-28 2010-05-28 Single B-cell cultivation method and specific antibody production
PCT/EP2011/058616 WO2011147903A1 (en) 2010-05-28 2011-05-26 Single b-cell cultivation method

Publications (1)

Publication Number Publication Date
MX345884B true MX345884B (es) 2017-02-22

Family

ID=42396427

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MX2012013437A MX2012013437A (es) 2010-05-28 2011-05-26 Metodo de cultivo de celulas b individuales.
MX2015001467A MX345884B (es) 2010-05-28 2011-05-26 Metodo de cultivo de celulas b individuales.

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MX2012013437A MX2012013437A (es) 2010-05-28 2011-05-26 Metodo de cultivo de celulas b individuales.

Country Status (15)

Country Link
US (4) US20130084637A1 (es)
EP (4) EP2400298B1 (es)
JP (3) JP5779239B2 (es)
KR (1) KR101495976B1 (es)
CN (2) CN105039252B (es)
BR (1) BR112012025695B1 (es)
CA (2) CA2798286C (es)
DK (1) DK2400298T3 (es)
ES (3) ES2434256T3 (es)
HK (2) HK1181112A1 (es)
MX (2) MX2012013437A (es)
PL (2) PL2400298T3 (es)
RU (1) RU2709531C2 (es)
SI (1) SI2400298T1 (es)
WO (1) WO2011147903A1 (es)

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JP6916884B2 (ja) * 2017-01-02 2021-08-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｂ細胞培養法
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CN108588019B (zh) * 2018-05-03 2019-04-02 首都医科大学附属北京朝阳医院 一种体外诱导初始b细胞分化为调节性b细胞的方法及其培养条件
CN109234232A (zh) * 2018-09-30 2019-01-18 杭州华安单抗生物技术有限公司 兔外周血b细胞的培养体系及培养方法、抗体的制备方法和应用
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