HK1181112A1 - Single -cell cultivation method - Google Patents

Single -cell cultivation method

Info

Publication number
HK1181112A1
HK1181112A1 HK13108297.8A HK13108297A HK1181112A1 HK 1181112 A1 HK1181112 A1 HK 1181112A1 HK 13108297 A HK13108297 A HK 13108297A HK 1181112 A1 HK1181112 A1 HK 1181112A1
Authority
HK
Hong Kong
Prior art keywords
cultivation method
cell cultivation
cell
cultivation
Prior art date
Application number
HK13108297.8A
Other languages
English (en)
Chinese (zh)
Inventor
Josef Endl
Natalie Schuhmacher
Sonja Offner
Josef Platzer
Basile Siewe
Irmgard Thorey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1181112A1 publication Critical patent/HK1181112A1/xx

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1114—T cells
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    • C12N2502/00—Coculture with; Conditioned medium produced by
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    • C12N2502/1157—Monocytes, macrophages
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    • C12N2502/00—Coculture with; Conditioned medium produced by
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    • C12N2502/1185—Thymus cells
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    • C12N2502/70—Non-animal cells
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    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
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    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells

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  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
HK13108297.8A 2010-05-28 2013-07-15 Single -cell cultivation method HK1181112A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10005602.7A EP2400298B1 (en) 2010-05-28 2010-05-28 Single B-cell cultivation method and specific antibody production
PCT/EP2011/058616 WO2011147903A1 (en) 2010-05-28 2011-05-26 Single b-cell cultivation method

Publications (1)

Publication Number Publication Date
HK1181112A1 true HK1181112A1 (en) 2013-11-01

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Family Applications (2)

Application Number Title Priority Date Filing Date
HK13108297.8A HK1181112A1 (en) 2010-05-28 2013-07-15 Single -cell cultivation method
HK16100556.8A HK1212731A1 (zh) 2010-05-28 2016-01-19 單個 細胞培養方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
HK16100556.8A HK1212731A1 (zh) 2010-05-28 2016-01-19 單個 細胞培養方法

Country Status (15)

Country Link
US (4) US20130084637A1 (xx)
EP (4) EP2400298B1 (xx)
JP (3) JP5779239B2 (xx)
KR (1) KR101495976B1 (xx)
CN (2) CN105039252B (xx)
BR (1) BR112012025695B1 (xx)
CA (2) CA2798286C (xx)
DK (1) DK2400298T3 (xx)
ES (3) ES2434256T3 (xx)
HK (2) HK1181112A1 (xx)
MX (2) MX2012013437A (xx)
PL (2) PL2400298T3 (xx)
RU (1) RU2709531C2 (xx)
SI (1) SI2400298T1 (xx)
WO (1) WO2011147903A1 (xx)

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JP6916884B2 (ja) * 2017-01-02 2021-08-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｂ細胞培養法
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CN108588019B (zh) * 2018-05-03 2019-04-02 首都医科大学附属北京朝阳医院 一种体外诱导初始b细胞分化为调节性b细胞的方法及其培养条件
CN109234232A (zh) * 2018-09-30 2019-01-18 杭州华安单抗生物技术有限公司 兔外周血b细胞的培养体系及培养方法、抗体的制备方法和应用
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BR112012025695A2 (pt) 2016-07-05
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HK1212731A1 (zh) 2016-06-17
PL3239708T3 (pl) 2021-06-14
EP3239708A1 (en) 2017-11-01
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