CN102918395B - 单个b细胞培养方法 - Google Patents

单个b细胞培养方法 Download PDF

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Publication number
CN102918395B
CN102918395B CN201180026559.6A CN201180026559A CN102918395B CN 102918395 B CN102918395 B CN 102918395B CN 201180026559 A CN201180026559 A CN 201180026559A CN 102918395 B CN102918395 B CN 102918395B
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cells
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cell
antibody
feeder
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CN102918395A (zh
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J·恩德尔
N·舒马赫尔
S·奥夫纳
J·普拉策尔
B·西韦
I·托雷
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F Hoffmann La Roche AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
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CN201180026559.6A 2010-05-28 2011-05-26 单个b细胞培养方法 Active CN102918395B (zh)

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Applications Claiming Priority (3)

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EP10005602.7A EP2400298B1 (en) 2010-05-28 2010-05-28 Single B-cell cultivation method and specific antibody production
EP10005602.7 2010-05-28
PCT/EP2011/058616 WO2011147903A1 (en) 2010-05-28 2011-05-26 Single b-cell cultivation method

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US (4) US20130084637A1 (enExample)
EP (4) EP2400298B1 (enExample)
JP (3) JP5779239B2 (enExample)
KR (1) KR101495976B1 (enExample)
CN (2) CN102918395B (enExample)
BR (1) BR112012025695B1 (enExample)
CA (2) CA2798286C (enExample)
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EP2727941A1 (en) 2012-11-05 2014-05-07 MAB Discovery GmbH Method for the production of multispecific antibodies
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EP2727942A1 (en) 2012-11-05 2014-05-07 MAB Discovery GmbH Bispecific antibodies against human EGFR, HER2, and HER3
EP2727943A1 (en) 2012-11-05 2014-05-07 MAB Discovery GmbH Trispecific antibodies against human EGFR, HER2 and HER3
DK2971039T3 (da) 2013-03-14 2020-04-06 Immusoft Corp Fremgangsmåder til in vitro-differentiering af hukommelses-b-celler og transduktion med vsv-g-pseudotypeomdannede virusvektorer
AU2014232225B2 (en) * 2013-03-15 2020-03-19 H. Lundbeck A/S. Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens
CN104232577B (zh) * 2013-06-20 2017-12-01 中国人民解放军军事医学科学院基础医学研究所 一种获得自身反应性b细胞的方法
DK3087177T3 (da) * 2013-12-24 2022-10-03 Kling Biotherapeutics B V Antistofproduktion ex vivo
CA2990305A1 (en) 2015-06-26 2016-12-29 Mab Discovery Gmbh Monoclonal anti-il-1racp antibodies
WO2017086419A1 (ja) 2015-11-18 2017-05-26 中外製薬株式会社 液性免疫応答の増強方法
EP4273232A3 (en) 2016-03-30 2024-01-03 F. Hoffmann-La Roche AG B-cell cultivation method
EP3241845A1 (en) 2016-05-06 2017-11-08 MAB Discovery GmbH Humanized anti-il-1r3 antibodies
CN106222137A (zh) * 2016-08-24 2016-12-14 南昌大学 一种体外活化人记忆性b细胞成浆细胞的培养方法
WO2018112407A1 (en) 2016-12-15 2018-06-21 Duke University Antibodies and methods for depleting regulatory b10 cells and use in combination with immune checkpoint inhibitors
JP6916884B2 (ja) * 2017-01-02 2021-08-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｂ細胞培養法
EP3401332A1 (en) 2017-05-08 2018-11-14 MAB Discovery GmbH Anti-il-1r3 antibodies for use in inflammatory conditions
WO2018210896A1 (en) 2017-05-19 2018-11-22 F. Hoffmann-La Roche Ag Method for the production of thymocyte supernatant
US12009521B2 (en) * 2017-11-08 2024-06-11 Sharp Kabushiki Kaisha Negative electrode for batteries, battery, and method for producing battery
CN111417719B (zh) * 2017-11-30 2023-08-29 豪夫迈·罗氏有限公司 B细胞培养方法
CN108588019B (zh) * 2018-05-03 2019-04-02 首都医科大学附属北京朝阳医院 一种体外诱导初始b细胞分化为调节性b细胞的方法及其培养条件
CN109234232A (zh) * 2018-09-30 2019-01-18 杭州华安单抗生物技术有限公司 兔外周血b细胞的培养体系及培养方法、抗体的制备方法和应用
CN113272327A (zh) 2018-12-30 2021-08-17 豪夫迈·罗氏有限公司 抗兔cd19抗体及其使用方法
WO2021211997A1 (en) * 2020-04-16 2021-10-21 The General Hospital Corporation B cell immunomodulatory therapy for acute respiratory distress syndrome
CN111518765B (zh) * 2020-05-12 2021-01-26 优睿赛思(武汉)生物科技有限公司 一种b淋巴细胞体外培养体系及应用
AU2023345467A1 (en) 2022-09-21 2025-05-08 Sanofi Biotechnology Humanized anti-il-1r3 antibody and methods of use
CN120917139A (zh) 2023-04-05 2025-11-07 豪夫迈·罗氏有限公司 用于抗体表达细胞的体外培养方法

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