JP4092222B2 - 多次元型液体クロマトグラフ分析装置 - Google Patents

多次元型液体クロマトグラフ分析装置 Download PDF

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JP4092222B2
JP4092222B2 JP2003039107A JP2003039107A JP4092222B2 JP 4092222 B2 JP4092222 B2 JP 4092222B2 JP 2003039107 A JP2003039107 A JP 2003039107A JP 2003039107 A JP2003039107 A JP 2003039107A JP 4092222 B2 JP4092222 B2 JP 4092222B2
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column
sample
mobile phase
analysis
concentration
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JP2003254955A5 (enExample
JP2003254955A (ja
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潤一 増田
ジェフリー・エイ・コワラク
エドワード・ジェイ・アンスワース
サンフォード・ピィ・マーケイ
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Shimadzu Corp
Government of the United States of America
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8411—Intermediate storage of effluent, including condensation on surface
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Sampling And Sample Adjustment (AREA)
JP2003039107A 2002-03-06 2003-02-18 多次元型液体クロマトグラフ分析装置 Expired - Lifetime JP4092222B2 (ja)

Applications Claiming Priority (2)

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US10/091,029 US6802967B2 (en) 2002-03-06 2002-03-06 Multi-dimension liquid chromatography separation system
US10/091,029 2002-03-06

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JP2003254955A JP2003254955A (ja) 2003-09-10
JP2003254955A5 JP2003254955A5 (enExample) 2005-10-27
JP4092222B2 true JP4092222B2 (ja) 2008-05-28

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JP (1) JP4092222B2 (enExample)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7010964B2 (en) * 2002-10-31 2006-03-14 Nanostream, Inc. Pressurized microfluidic devices with optical detection regions
JP3816883B2 (ja) * 2003-03-06 2006-08-30 株式会社日立ハイテクノロジーズ 液体クロマトグラフ質量分析装置
US20070134808A1 (en) * 2003-07-29 2007-06-14 Sullivan James T Simultaneous multi-column liquid chromatograph for direct sampling of an array of liquid samples
CA2536809A1 (en) * 2003-09-05 2005-03-17 Sumitomo Chemical Company, Limited Liquid chromatographic apparatus
JP4166165B2 (ja) * 2004-01-30 2008-10-15 株式会社島津製作所 液体クロマトグラフ
FR2867089B1 (fr) * 2004-03-03 2006-06-09 Bionisis Sa Installation de separation de composants dans plusieurs voies paralleles
JP4093201B2 (ja) * 2004-03-30 2008-06-04 株式会社島津製作所 液体クロマトグラフ
US20050247625A1 (en) * 2004-05-05 2005-11-10 Jianbo Liu Determining conditions for chromatography
US7686959B2 (en) 2004-05-05 2010-03-30 Biotage Ab Control system and method for flash separation
ATE388397T1 (de) * 2004-07-03 2008-03-15 Hoffmann La Roche Vorkonzentrationsverbindung zur kopplung von kapillarer elektrochromatografie und kapillarzonenelektrophorese
US20060156792A1 (en) * 2004-10-12 2006-07-20 Yongdong Wang Multi-dimensional liquid chromatography separation system and method
JP2006125856A (ja) * 2004-10-26 2006-05-18 Sumitomo Chemical Co Ltd 液体クロマトグラフィー装置
CN101115540A (zh) * 2004-12-08 2008-01-30 谢佳辉 用于液相色谱的组合柱以及相关系统和方法
US8123949B2 (en) * 2005-01-20 2012-02-28 Waters Technologies Corporation Methods for separating compounds
JP4498186B2 (ja) * 2005-03-24 2010-07-07 株式会社日立ハイテクノロジーズ 液体クロマトグラフ分析方法及び装置
JP4467454B2 (ja) * 2005-03-31 2010-05-26 株式会社日立ハイテクノロジーズ 多次元液体クロマトグラフ及びそれを用いた分析方法
WO2007016971A1 (en) * 2005-07-25 2007-02-15 Agilent Technologies, Inc. Fluidic analysis with hydrophobic and hydrophilic compounds trapping
US20070140918A1 (en) * 2005-12-19 2007-06-21 Hongfeng Yin Fluidic separation devices and methods with reduced sample broadening
US20070178581A1 (en) * 2006-01-27 2007-08-02 Shimadzu Corporation Screening method and apparatus for identifying a reactive compound bonding with a target compound
JP4790435B2 (ja) * 2006-02-03 2011-10-12 株式会社日立ハイテクノロジーズ ３次元液体クロマトグラフィ
US20070246063A1 (en) * 2006-04-21 2007-10-25 Macronix International Co., Ltd. Method of performing a pressure calibration during waferless autoclean process
EP1879026A1 (en) * 2006-06-24 2008-01-16 Agilent Technologies, Inc. Focussing a sample on an analytical column
JP5012148B2 (ja) * 2007-04-03 2012-08-29 株式会社島津製作所 液体クロマトグラフ
WO2008150763A1 (en) * 2007-05-29 2008-12-11 Waters Investments Limited Apparatus and methods for multidimensional analysis
US9091695B2 (en) * 2007-06-01 2015-07-28 Laboratory Corporation Of America Holdings Methods and systems for quantification of peptides and other analytes
JP5002365B2 (ja) 2007-08-06 2012-08-15 株式会社日立製作所 質量分析装置及び質量分析方法
EP2068143A1 (de) * 2007-12-07 2009-06-10 Roche Diagnostics GmbH Manipulation magnetischer Mikropartikel in einem Hochdruck-Flüssigkeitssystem und Extraktionsprozess
JP4609494B2 (ja) * 2008-01-07 2011-01-12 株式会社島津製作所 液体クロマトグラフ
JP2010014429A (ja) * 2008-07-01 2010-01-21 Shimadzu Corp 液体クロマトグラフ
US8337705B2 (en) * 2008-12-04 2012-12-25 Roche Diagnostics Operations, Inc. Manipulation of magnetic microparticles in a high pressure liquid system and extraction process
KR20110114560A (ko) * 2008-12-10 2011-10-19 올테크 어소시에이츠, 인크. 크로마토그래피 시스템용 용매 공급 시스템 및 제작 방법 및 이용 방법
WO2010083147A1 (en) 2009-01-14 2010-07-22 Waters Technologies Corporation Rotating valve
US20100288443A1 (en) * 2009-03-13 2010-11-18 Richard Earl Tucker Tuckers e.z. all purpose taping tool
JP5195686B2 (ja) * 2009-08-05 2013-05-08 株式会社島津製作所 液体クロマトグラフ装置
CN203577372U (zh) * 2010-10-29 2014-05-07 赛默菲尼根有限责任公司 模块式多柱色谱盒
EP2689244B1 (en) 2011-03-23 2020-06-17 Regents of the University of Minnesota Valve and splitting system for multi-dimensional liquid analysis
US9228982B2 (en) * 2011-09-16 2016-01-05 Agilent Technologies, Inc. Single injection valve for HPLC combining sample introduction, wash cycles and diagnosis
NL2008483C2 (en) 2012-03-13 2013-09-16 Univ Leiden Method and device for solvent evaporation from a liquid feed.
EP2825864B1 (en) 2012-03-13 2022-08-31 Universiteit Leiden Method and device for solvent evaporation from a liquid feed
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
JP6232067B2 (ja) * 2012-08-31 2017-11-15 西安奥▲嵐▼科技▲開▼▲発▼有限▲責▼任公司 タンパク質分離用多次元の液体クロマトグラフィーの分離システム及分離方法
CN103063753B (zh) * 2012-08-31 2015-07-29 西安奥岚科技开发有限责任公司 用于蛋白分离的多维液相色谱分离系统及分离方法
CN103808817A (zh) * 2012-11-12 2014-05-21 中国科学院大连化学物理研究所 一种成倍提高高效液相色谱仪分析通量的结构
US9404900B2 (en) * 2012-12-21 2016-08-02 Thermo Finnigan Llc System for analysis of phase-I and phase-II metabolites and parent compounds without hydrolysis
US9679757B2 (en) * 2013-04-12 2017-06-13 Waters Technologies Corporation Liquid chromatography systems and methods
US9683975B2 (en) 2014-02-12 2017-06-20 Idex Health & Science Llc Volumetric flow regulation in multi-dimensional liquid analysis systems
KR101617615B1 (ko) * 2014-03-28 2016-05-03 고려대학교 산학협력단 이중 온라인 액체크로마토그래피 장치 및 그 제어 방법
JP6372147B2 (ja) * 2014-04-23 2018-08-15 株式会社島津製作所 タンパク質に対する親和性評価のための分析方法及びその装置
WO2016127330A1 (zh) * 2015-02-11 2016-08-18 王峰 一种二维液相色谱仪
CN106645506B (zh) * 2015-08-20 2019-04-09 中国科学院大连化学物理研究所 一种三维液相色谱分离系统
US10585071B2 (en) * 2015-11-12 2020-03-10 Michael Organ Multidimensional peak purity analysis
KR101795747B1 (ko) * 2016-01-15 2017-11-08 고려대학교 산학협력단 비연속적 시료 분획 및 통합유닛과 이를 구비한 이중 온라인 다기능 액체크로마토그래피 장치
DE102017101012A1 (de) 2016-01-22 2017-07-27 Waters Technologies Corporation At-column dilution verwendendes mehrdimensionales chromatographiesystem
DE102017101427A1 (de) * 2016-01-25 2017-07-27 Waters Technologies Corporation Mehrdimensionales Chromatographiesystem zur Analyse multipler Probenkomponenten
JP6716087B2 (ja) * 2016-03-25 2020-07-01 公立大学法人大阪 分析方法及び分析装置
CN106075954B (zh) * 2016-05-25 2018-04-27 华南理工大学 以一个多通阀为切换装置的停流型二维液相色谱及其应用
CN105891369B (zh) * 2016-05-25 2017-12-01 华南理工大学 以两个多通阀为切换装置的停流型二维液相色谱及其应用
CN105938130B (zh) * 2016-06-30 2018-04-20 朱靖博 一种集分离方法开发、在线分离‑富集于一体的天然药物二维制备色谱仪及其工作方法
CN107037169B (zh) * 2016-11-01 2018-12-04 聊城大学 一种带多功能切换阀的全二维高效液相色谱仪及色谱分离方法
CN106501429B (zh) * 2016-11-01 2018-03-09 聊城大学 一种带多功能切换阀的多通道全二维高效液相色谱仪及用途
JP6790963B2 (ja) * 2017-03-30 2020-11-25 株式会社島津製作所 液体クロマトグラフ
JP6933343B2 (ja) * 2017-04-13 2021-09-08 株式会社島津製作所 液体クロマトグラフ質量分析による試料分析方法
US20210055270A1 (en) * 2017-12-27 2021-02-25 Shimadzu Corporation Two-dimensional liquid chromatograph system
CN108562678B (zh) * 2018-02-12 2023-09-22 大连博迈科技发展有限公司 基于同一检测器的全在线检测的三维液相色谱分离系统
US12130267B2 (en) * 2018-11-28 2024-10-29 Shimadzu Corporation Analyzing method for azo compound
JPWO2021214916A1 (enExample) * 2020-04-22 2021-10-28
EP4147040B1 (en) * 2020-05-07 2025-06-25 Waters Technologies Corporation Multidimensional liquid chromatography system
EP3910329B1 (en) 2020-05-14 2025-08-20 LG Chem, Ltd. On-line system for improving detection level of analytes by liquid chromatography and analysis method using same
KR102572670B1 (ko) * 2020-05-14 2023-08-30 주식회사 엘지화학 액체크로마토그래피에 의한 분석 물질의 검출 감도 향상을 위한 온라인 시스템 및 이를 이용한 분석 방법
US12458908B2 (en) * 2020-11-30 2025-11-04 PAK Biosolutions Inc. End-to-end continuous purification system
JP7548414B2 (ja) * 2021-03-09 2024-09-10 株式会社島津製作所 分取液体クロマトグラフ
CN116008416A (zh) * 2022-12-13 2023-04-25 北京豪思生物科技股份有限公司 液相色谱仪
CN116203167B (zh) * 2023-04-27 2023-08-01 广东省农业科学院蚕业与农产品加工研究所 一种循环型多维液相色谱分离系统及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4314823A (en) * 1979-03-05 1982-02-09 Dionex Corporation Combination apparatus and method for chromatographic separation and quantitative analysis of multiple ionic species
FR2527934A1 (fr) * 1982-06-03 1983-12-09 Elf Aquitaine Procede de fractionnement de melanges par chromatographie d'elution avec fluide en etat supercritique et installation pour sa mise en oeuvre
US4544485A (en) * 1984-08-31 1985-10-01 Purdue Research Foundation Chromatographic method and means
US4597943A (en) * 1984-11-29 1986-07-01 Morinaga & Co., Ltd. Apparatus for analyzing solid sample with supercritical fluid
JPS62138753A (ja) * 1985-12-12 1987-06-22 Hitachi Ltd 液体クロマトグラフイによる分画・分取方法および装置
JP2892795B2 (ja) * 1989-09-12 1999-05-17 エーザイ株式会社 高速液体クロマトグラフィー質量分析における移動相の変換方法と装置
US5372716A (en) * 1991-02-28 1994-12-13 Suprex Corporation Supercritical fluid extraction coupled to analytical chromatography system
US5458783A (en) * 1992-08-13 1995-10-17 Suprex Corporation Supercritical fluid extraction coupled to analytical chromatography system
US5449902A (en) * 1992-12-17 1995-09-12 Hitachi, Ltd. Apparatus for directly coupling analytical column with mass spectrometer
US5403386A (en) * 1993-10-14 1995-04-04 Rohm And Haas Company Concentrator apparatus for detecting trace organic components in aqueous samples
US5935443A (en) * 1995-03-03 1999-08-10 Alltech Associates, Inc. Electrochemically regenerated ion neutralization and concentration devices and systems
US5720798A (en) * 1996-04-30 1998-02-24 Hewlett-Packard Company Micromachined analyte trap for gas phase streams

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US20030168392A1 (en) 2003-09-11
JP2003254955A (ja) 2003-09-10

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