JP2019524134A5 - - Google Patents

Info

Publication number

JP2019524134A5

JP2019524134A5 JP2019507224A JP2019507224A JP2019524134A5 JP 2019524134 A5 JP2019524134 A5 JP 2019524134A5 JP 2019507224 A JP2019507224 A JP 2019507224A JP 2019507224 A JP2019507224 A JP 2019507224A JP 2019524134 A5 JP2019524134 A5 JP 2019524134A5

Authority

JP

Japan

Prior art keywords

seq

amino acid

udp glycosyltransferase

host cell

loop

Prior art date

2017-08-11

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 125000003275 alpha amino acid group Chemical group 0.000 claims 62
• 102000051366 Glycosyltransferases Human genes 0.000 claims 41
• 108700023372 Glycosyltransferases Proteins 0.000 claims 41
• 235000001014 amino acid Nutrition 0.000 claims 32
• 108020004707 nucleic acids Proteins 0.000 claims 6
• 102000039446 nucleic acids Human genes 0.000 claims 6
• 150000007523 nucleic acids Chemical class 0.000 claims 6
• 241000954177 Bangana ariza Species 0.000 claims 4
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 4
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 4
• 239000004383 Steviol glycoside Substances 0.000 claims 4
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 4
• 235000013922 glutamic acid Nutrition 0.000 claims 4
• 239000004220 glutamic acid Substances 0.000 claims 4
• RPYRMTHVSUWHSV-CUZJHZIBSA-N rebaudioside D Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RPYRMTHVSUWHSV-CUZJHZIBSA-N 0.000 claims 4
• 229930182488 steviol glycoside Natural products 0.000 claims 4
• 235000019411 steviol glycoside Nutrition 0.000 claims 4
• 235000019202 steviosides Nutrition 0.000 claims 4
• 239000004474 valine Substances 0.000 claims 4
• 102000004190 Enzymes Human genes 0.000 claims 3
• 108090000790 Enzymes Proteins 0.000 claims 3
• 230000037361 pathway Effects 0.000 claims 3
• 150000008144 steviol glycosides Chemical class 0.000 claims 3
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 2
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 2
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 2
• 125000000539 amino acid group Chemical group 0.000 claims 2
• 230000002538 fungal effect Effects 0.000 claims 2
• 229960000310 isoleucine Drugs 0.000 claims 2
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims 2
• 238000004519 manufacturing process Methods 0.000 claims 2
• 238000002864 sequence alignment Methods 0.000 claims 2
• ONVABDHFQKWOSV-UHFFFAOYSA-N 16-Phyllocladene Natural products C1CC(C2)C(=C)CC32CCC2C(C)(C)CCCC2(C)C31 ONVABDHFQKWOSV-UHFFFAOYSA-N 0.000 claims 1
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
• 102000007317 Farnesyltranstransferase Human genes 0.000 claims 1
• 108010007508 Farnesyltranstransferase Proteins 0.000 claims 1
• 241000238631 Hexapoda Species 0.000 claims 1
• 102000008109 Mixed Function Oxygenases Human genes 0.000 claims 1
• 108010074633 Mixed Function Oxygenases Proteins 0.000 claims 1
• 108010045510 NADPH-Ferrihemoprotein Reductase Proteins 0.000 claims 1
• 108090000854 Oxidoreductases Proteins 0.000 claims 1
• 102000004316 Oxidoreductases Human genes 0.000 claims 1
• 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
• 101100427140 Stevia rebaudiana UGT74G1 gene Proteins 0.000 claims 1
• 101100262416 Stevia rebaudiana UGT76G1 gene Proteins 0.000 claims 1
• 101100048059 Stevia rebaudiana UGT85C2 gene Proteins 0.000 claims 1
• QFVOYBUQQBFCRH-UHFFFAOYSA-N Steviol Natural products C1CC2(C3)CC(=C)C3(O)CCC2C2(C)C1C(C)(C(O)=O)CCC2 QFVOYBUQQBFCRH-UHFFFAOYSA-N 0.000 claims 1
• 230000001580 bacterial effect Effects 0.000 claims 1
• 229910052799 carbon Inorganic materials 0.000 claims 1
• 150000001875 compounds Chemical class 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 239000001177 diphosphate Substances 0.000 claims 1
• XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 1
• 235000011180 diphosphates Nutrition 0.000 claims 1
• 238000000855 fermentation Methods 0.000 claims 1
• 230000004151 fermentation Effects 0.000 claims 1
• 239000008103 glucose Substances 0.000 claims 1
• 108700014210 glycosyltransferase activity proteins Proteins 0.000 claims 1
• 238000000034 method Methods 0.000 claims 1
• 239000002773 nucleotide Substances 0.000 claims 1
• 125000003729 nucleotide group Chemical group 0.000 claims 1
• 229920001184 polypeptide Polymers 0.000 claims 1
• 108090000765 processed proteins & peptides Proteins 0.000 claims 1
• 102000004196 processed proteins & peptides Human genes 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 claims 1
• QFVOYBUQQBFCRH-VQSWZGCSSA-N steviol Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)CC1)C[C@H]2[C@@]2(C)[C@H]1[C@](C)(C(O)=O)CCC2 QFVOYBUQQBFCRH-VQSWZGCSSA-N 0.000 claims 1
• 229940032084 steviol Drugs 0.000 claims 1