JP2017511148A5 - - Google Patents
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- JP2017511148A5 JP2017511148A5 JP2016562915A JP2016562915A JP2017511148A5 JP 2017511148 A5 JP2017511148 A5 JP 2017511148A5 JP 2016562915 A JP2016562915 A JP 2016562915A JP 2016562915 A JP2016562915 A JP 2016562915A JP 2017511148 A5 JP2017511148 A5 JP 2017511148A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- seq
- host cell
- amino acid
- functional homologue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004169 proteins and genes Human genes 0.000 claims 31
- 108090000623 proteins and genes Proteins 0.000 claims 31
- 102000040430 polynucleotide Human genes 0.000 claims 19
- 108091033319 polynucleotide Proteins 0.000 claims 19
- 239000002157 polynucleotide Substances 0.000 claims 19
- 125000003275 alpha amino acid group Chemical group 0.000 claims 11
- 150000001413 amino acids Chemical class 0.000 claims 7
- 238000000034 method Methods 0.000 claims 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
- 238000004113 cell culture Methods 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 230000002018 overexpression Effects 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 241001138401 Kluyveromyces lactis Species 0.000 claims 1
- 241001099157 Komagataella Species 0.000 claims 1
- 241000235058 Komagataella pastoris Species 0.000 claims 1
- 241000320412 Ogataea angusta Species 0.000 claims 1
- 241001452677 Ogataea methanolica Species 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 claims 1
- 241000499912 Trichoderma reesei Species 0.000 claims 1
- 241000222124 [Candida] boidinii Species 0.000 claims 1
- 239000003674 animal food additive Substances 0.000 claims 1
- 239000002778 food additive Substances 0.000 claims 1
- 235000013373 food additive Nutrition 0.000 claims 1
- 230000014509 gene expression Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14165186.9 | 2014-04-17 | ||
| EP14165186 | 2014-04-17 | ||
| PCT/EP2015/058242 WO2015158808A2 (en) | 2014-04-17 | 2015-04-16 | Recombinant host cell engineered to overexpress helper proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019236345A Division JP7062633B2 (ja) | 2014-04-17 | 2019-12-26 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017511148A JP2017511148A (ja) | 2017-04-20 |
| JP2017511148A5 true JP2017511148A5 (enExample) | 2018-05-17 |
Family
ID=50513080
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016562915A Pending JP2017511148A (ja) | 2014-04-17 | 2015-04-16 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
| JP2019236345A Active JP7062633B2 (ja) | 2014-04-17 | 2019-12-26 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019236345A Active JP7062633B2 (ja) | 2014-04-17 | 2019-12-26 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10865416B2 (enExample) |
| EP (2) | EP4311857A3 (enExample) |
| JP (2) | JP2017511148A (enExample) |
| KR (1) | KR102373954B1 (enExample) |
| CN (1) | CN106661541B (enExample) |
| AU (1) | AU2015248815B2 (enExample) |
| BR (1) | BR112016023304A2 (enExample) |
| CA (1) | CA2948369C (enExample) |
| IL (1) | IL248376B (enExample) |
| SG (2) | SG10201809146QA (enExample) |
| WO (1) | WO2015158808A2 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2015248807B2 (en) | 2014-04-17 | 2021-07-22 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Recombinant host cell for expressing proteins of interest |
| MX2017006127A (es) | 2014-11-11 | 2017-11-08 | Clara Foods Co | Metodos y composiciones para produccion de proteina de clara de huevo. |
| CN110612352B (zh) * | 2017-03-29 | 2025-01-24 | 贝林格尔·英格海姆Rcv两合公司 | 具有改变的膜脂组成的重组宿主细胞 |
| CN109722445A (zh) * | 2017-10-27 | 2019-05-07 | 成都必高生物科技有限公司 | 提升溶菌酶产量及活性的方法 |
| BR112020010081A2 (pt) * | 2017-11-20 | 2020-11-03 | Axxence Holding B.V. | produção de um composto aromatizante em uma célula hospedeira |
| JP7623680B2 (ja) | 2018-05-17 | 2025-01-29 | ボルト スレッズ インコーポレイテッド | 組換えタンパク質の分泌を改善するためのｓｅｃ改変株 |
| US20210269811A1 (en) * | 2018-06-27 | 2021-09-02 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Means and methods for increased protein expression by use of transcription factors |
| CN112739819A (zh) | 2018-09-24 | 2021-04-30 | 中东科技大学 | 利用启动子工程化设计醇脱氢酶2(adh2)启动子变体 |
| CN109439640B (zh) * | 2018-10-09 | 2021-07-20 | 天津科技大学 | 伏马毒素降解酶FumDSB及其基因和应用 |
| CN109439639B (zh) * | 2018-10-09 | 2021-07-20 | 天津科技大学 | 伏马毒素降解酶FumDPS及其基因和应用 |
| CN111378585B (zh) | 2018-12-28 | 2023-06-16 | 丰益(上海)生物技术研发中心有限公司 | 用于表达外源基因的毕赤酵母突变株 |
| KR102495283B1 (ko) | 2019-01-11 | 2023-02-06 | 론자 리미티드 | 재조합 숙주 세포에서 탄소 공급원 조절된 단백질 생산 |
| HRP20250315T1 (hr) | 2019-07-11 | 2025-06-06 | Clara Foods Co. | Sastav napitka koji sadrži rekombinantni protein ovomukoid |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| JP2023512309A (ja) * | 2020-02-04 | 2023-03-24 | クララ フーズ カンパニー | 高収量組換え微生物のためのシステムおよび方法ならびにその使用 |
| CN111349177B (zh) * | 2020-03-13 | 2022-04-22 | 西北农林科技大学 | 一种融合抗菌肽cat的制备方法和应用 |
| CN111607575B (zh) * | 2020-04-07 | 2022-10-04 | 天津科技大学 | 一种转氨酶phta、制备方法和应用 |
| CN111549007B (zh) * | 2020-04-07 | 2022-10-04 | 天津科技大学 | 一种转氨酶tsta、制备方法和应用 |
| CN113088533B (zh) * | 2021-04-15 | 2023-03-24 | 华中科技大学 | 一种高效表达藤壶粘胶蛋白的酵母工程菌及其制备方法 |
| CN113801801B (zh) * | 2021-10-08 | 2023-03-07 | 枣庄市杰诺生物酶有限公司 | 一株高效生产碱性果胶酶的重组菌及其应用 |
| CN118147180A (zh) * | 2024-05-09 | 2024-06-07 | 芝诺(苏州)生物科技有限公司 | 一种代谢工程改造毕赤酵母合成人乳铁蛋白的方法 |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4546082A (en) | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US4601893A (en) | 1984-02-08 | 1986-07-22 | Pfizer Inc. | Laminate device for controlled and prolonged release of substances to an ambient environment and method of use |
| GB8726953D0 (en) | 1987-11-18 | 1987-12-23 | Delta Biotechnology Ltd | Yeast expression system |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| EP0463056A1 (en) | 1989-03-17 | 1992-01-02 | E.I. Du Pont De Nemours And Company | External regulation of gene expression |
| US5194600A (en) * | 1990-03-05 | 1993-03-16 | Royal Institute For The Advancement Of Learning | Genes which participate in β-glucan assembly and use thereof |
| DE4027453A1 (de) | 1990-08-30 | 1992-03-05 | Degussa | Neue plasmide aus corynebacterium glutamicum und davon abgeleitete plasmidvektoren |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| US6291205B1 (en) | 1992-06-12 | 2001-09-18 | Merck & Co., Inc. | Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae |
| WO1994008012A1 (en) | 1992-10-02 | 1994-04-14 | Research Corporation Technologies, Inc. | Methods for increasing secretion of overexpressed proteins |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| DE69534530T2 (de) | 1994-08-12 | 2006-07-06 | Immunomedics, Inc. | Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper |
| US6068995A (en) * | 1994-10-25 | 2000-05-30 | Yoshitomi Pharmaceutical Industries, Ltd. | Method for producing protein |
| DE4440118C1 (de) | 1994-11-11 | 1995-11-09 | Forschungszentrum Juelich Gmbh | Die Genexpression in coryneformen Bakterien regulierende DNA |
| GB9515941D0 (en) | 1995-08-03 | 1995-10-04 | Zeneca Ltd | DNA constructs |
| GB9516241D0 (en) | 1995-08-08 | 1995-10-11 | Zeneca Ltd | Dna constructs |
| JPH10229891A (ja) | 1997-02-20 | 1998-09-02 | Mitsubishi Rayon Co Ltd | マロン酸誘導体の製造法 |
| FI972909A0 (fi) | 1997-07-09 | 1997-07-09 | Valtion Teknillinen | Oekad produktion av avsoendrade proteiner genom rekombinatjaestceller |
| US7033781B1 (en) * | 1999-09-29 | 2006-04-25 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
| WO2001072783A2 (en) | 2000-03-24 | 2001-10-04 | Genencor International, Inc. | Production of secreted proteins by recombinant eukaryotic cells |
| GB0104685D0 (en) * | 2001-02-26 | 2001-04-11 | Leuven K U Res & Dev | Tau-opathy model |
| JP2005500841A (ja) | 2001-07-27 | 2005-01-13 | アメリカ合衆国 | オリゴヌクレオチドを用いるインビボ部位指定変異誘発のためのシステム |
| US9057061B2 (en) | 2003-12-23 | 2015-06-16 | Novozymes Biopharma Dk A/S | Gene expression technique |
| GB0329681D0 (en) | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Gene expression technique |
| SE526220C2 (sv) | 2003-12-23 | 2005-08-02 | Kvaerner Pulping Tech | Anordning samt förfarande för att mata ut cellulosamassa från ett cylindriskt lagringstorn |
| AU2005254734B9 (en) | 2004-06-16 | 2011-10-13 | Dsm Ip Assets B.V. | Production of polypeptides by improved secretion |
| US7785872B2 (en) * | 2004-12-08 | 2010-08-31 | Simpson Biotech Co., Ltd. | Nucleic acids for enhancing gene expression and use thereof |
| AU2008241061A1 (en) | 2007-04-20 | 2008-10-30 | Polymun Scientific Immunbiologische Forschung Gmbh | Yeast expression systems |
| EP2198028A2 (en) * | 2007-08-31 | 2010-06-23 | BASF Plant Science GmbH | Method for producing a transgenic plant cell, a plant or a part thereof with increased resistance to plant disease |
| AU2008318644B2 (en) | 2007-11-01 | 2013-05-02 | Danisco Us Inc. | Signal sequences and co-expressed chaperones for improving protein production in a host cell |
| DE102008035152A1 (de) | 2008-07-28 | 2010-02-04 | Evocatal Gmbh | Chaperon-Toolbox |
| US8440456B2 (en) | 2009-05-22 | 2013-05-14 | Vib, Vzw | Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins |
| KR101930961B1 (ko) * | 2010-02-24 | 2018-12-19 | 머크 샤프 앤드 돔 코포레이션 | 피키아 파스토리스에서 생산된 치료 당단백질 상의 ｎ-글리코실화 부위 점유를 증가시키는 방법 |
| CN102220254B (zh) * | 2010-04-14 | 2013-04-17 | 新疆农业科学院生物质能源研究所 | 一种重组酿酒酵母工程菌株及其应用 |
| JP6295512B2 (ja) | 2012-03-15 | 2018-03-20 | 株式会社豊田中央研究所 | 酵母における外来遺伝子の発現産物の生産方法、酵母における発現調節剤及びその利用 |
| KR101472273B1 (ko) | 2012-03-29 | 2014-12-24 | 엘에스엠 주식회사 | ＣＧＴａｓｅ의 세포 표면 발현 재조합 벡터 |
| AU2015248807B2 (en) * | 2014-04-17 | 2021-07-22 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Recombinant host cell for expressing proteins of interest |
-
2015
- 2015-04-16 AU AU2015248815A patent/AU2015248815B2/en active Active
- 2015-04-16 EP EP23205278.7A patent/EP4311857A3/en active Pending
- 2015-04-16 KR KR1020167032074A patent/KR102373954B1/ko active Active
- 2015-04-16 SG SG10201809146QA patent/SG10201809146QA/en unknown
- 2015-04-16 SG SG11201608084TA patent/SG11201608084TA/en unknown
- 2015-04-16 BR BR112016023304A patent/BR112016023304A2/pt not_active Application Discontinuation
- 2015-04-16 EP EP15721134.3A patent/EP3132028B1/en active Active
- 2015-04-16 WO PCT/EP2015/058242 patent/WO2015158808A2/en not_active Ceased
- 2015-04-16 CA CA2948369A patent/CA2948369C/en active Active
- 2015-04-16 US US15/304,050 patent/US10865416B2/en active Active
- 2015-04-16 CN CN201580032989.7A patent/CN106661541B/zh active Active
- 2015-04-16 JP JP2016562915A patent/JP2017511148A/ja active Pending
-
2016
- 2016-10-18 IL IL248376A patent/IL248376B/en active IP Right Grant
-
2019
- 2019-12-26 JP JP2019236345A patent/JP7062633B2/ja active Active
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