JP2017511148A5 - - Google Patents
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- Publication number
- JP2017511148A5 JP2017511148A5 JP2016562915A JP2016562915A JP2017511148A5 JP 2017511148 A5 JP2017511148 A5 JP 2017511148A5 JP 2016562915 A JP2016562915 A JP 2016562915A JP 2016562915 A JP2016562915 A JP 2016562915A JP 2017511148 A5 JP2017511148 A5 JP 2017511148A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- seq
- host cell
- amino acid
- functional homologue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004169 proteins and genes Human genes 0.000 claims 31
- 108090000623 proteins and genes Proteins 0.000 claims 31
- 102000040430 polynucleotide Human genes 0.000 claims 19
- 108091033319 polynucleotide Proteins 0.000 claims 19
- 239000002157 polynucleotide Substances 0.000 claims 19
- 125000003275 alpha amino acid group Chemical group 0.000 claims 11
- 150000001413 amino acids Chemical class 0.000 claims 7
- 238000000034 method Methods 0.000 claims 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
- 238000004113 cell culture Methods 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 230000002018 overexpression Effects 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 241001138401 Kluyveromyces lactis Species 0.000 claims 1
- 241001099157 Komagataella Species 0.000 claims 1
- 241000235058 Komagataella pastoris Species 0.000 claims 1
- 241000320412 Ogataea angusta Species 0.000 claims 1
- 241001452677 Ogataea methanolica Species 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 claims 1
- 241000499912 Trichoderma reesei Species 0.000 claims 1
- 241000222124 [Candida] boidinii Species 0.000 claims 1
- 239000003674 animal food additive Substances 0.000 claims 1
- 239000002778 food additive Substances 0.000 claims 1
- 235000013373 food additive Nutrition 0.000 claims 1
- 230000014509 gene expression Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14165186 | 2014-04-17 | ||
| EP14165186.9 | 2014-04-17 | ||
| PCT/EP2015/058242 WO2015158808A2 (en) | 2014-04-17 | 2015-04-16 | Recombinant host cell engineered to overexpress helper proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019236345A Division JP7062633B2 (ja) | 2014-04-17 | 2019-12-26 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017511148A JP2017511148A (ja) | 2017-04-20 |
| JP2017511148A5 true JP2017511148A5 (enExample) | 2018-05-17 |
Family
ID=50513080
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016562915A Pending JP2017511148A (ja) | 2014-04-17 | 2015-04-16 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
| JP2019236345A Active JP7062633B2 (ja) | 2014-04-17 | 2019-12-26 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019236345A Active JP7062633B2 (ja) | 2014-04-17 | 2019-12-26 | ヘルパータンパク質を過剰発現するように改変された組換え宿主細胞 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10865416B2 (enExample) |
| EP (2) | EP3132028B1 (enExample) |
| JP (2) | JP2017511148A (enExample) |
| KR (1) | KR102373954B1 (enExample) |
| CN (1) | CN106661541B (enExample) |
| AU (1) | AU2015248815B2 (enExample) |
| BR (1) | BR112016023304A2 (enExample) |
| CA (1) | CA2948369C (enExample) |
| IL (1) | IL248376B (enExample) |
| SG (2) | SG11201608084TA (enExample) |
| WO (1) | WO2015158808A2 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201809152QA (en) | 2014-04-17 | 2018-11-29 | Boehringer Ingelheim Rcv Gmbh | Recombinant host cell for expressing proteins of interest |
| CN114365792A (zh) | 2014-11-11 | 2022-04-19 | 克莱拉食品公司 | 用于生成卵清蛋白的方法和组合物 |
| JP7189145B2 (ja) * | 2017-03-29 | 2022-12-13 | ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 改変された膜脂質組成を有するリコンビナントホスト細胞 |
| CN109722445A (zh) * | 2017-10-27 | 2019-05-07 | 成都必高生物科技有限公司 | 提升溶菌酶产量及活性的方法 |
| JP2021503307A (ja) * | 2017-11-20 | 2021-02-12 | アクセンス ホールディング ビー．ヴイ．Ａｘｘｅｎｃｅ Ｈｏｌｄｉｎｇ Ｂ．Ｖ． | 宿主細胞におけるフレーバー化合物の生成 |
| WO2019222633A1 (en) | 2018-05-17 | 2019-11-21 | Bolt Threads, Inc. | Sec modified strains for improved secretion of recombinant proteins |
| WO2020002494A1 (en) * | 2018-06-27 | 2020-01-02 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Means and methods for increased protein expression by use of transcription factors |
| CN112739819A (zh) | 2018-09-24 | 2021-04-30 | 中东科技大学 | 利用启动子工程化设计醇脱氢酶2(adh2)启动子变体 |
| CN109439640B (zh) * | 2018-10-09 | 2021-07-20 | 天津科技大学 | 伏马毒素降解酶FumDSB及其基因和应用 |
| CN109439639B (zh) * | 2018-10-09 | 2021-07-20 | 天津科技大学 | 伏马毒素降解酶FumDPS及其基因和应用 |
| CN111378585B (zh) * | 2018-12-28 | 2023-06-16 | 丰益(上海)生物技术研发中心有限公司 | 用于表达外源基因的毕赤酵母突变株 |
| WO2020144313A1 (en) * | 2019-01-11 | 2020-07-16 | Lonza Ltd | Carbon-source regulated protein production in a recombinant host cell |
| HRP20250315T1 (hr) | 2019-07-11 | 2025-06-06 | Clara Foods Co. | Sastav napitka koji sadrži rekombinantni protein ovomukoid |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CA3165286A1 (en) * | 2020-02-04 | 2021-08-12 | Clara Foods Co. | Systems and methods for high yielding recombinant microorganisms and uses thereof |
| CN111349177B (zh) * | 2020-03-13 | 2022-04-22 | 西北农林科技大学 | 一种融合抗菌肽cat的制备方法和应用 |
| CN111607575B (zh) * | 2020-04-07 | 2022-10-04 | 天津科技大学 | 一种转氨酶phta、制备方法和应用 |
| CN111549007B (zh) * | 2020-04-07 | 2022-10-04 | 天津科技大学 | 一种转氨酶tsta、制备方法和应用 |
| CN113088533B (zh) * | 2021-04-15 | 2023-03-24 | 华中科技大学 | 一种高效表达藤壶粘胶蛋白的酵母工程菌及其制备方法 |
| CN113801801B (zh) * | 2021-10-08 | 2023-03-07 | 枣庄市杰诺生物酶有限公司 | 一株高效生产碱性果胶酶的重组菌及其应用 |
| CN118147180A (zh) * | 2024-05-09 | 2024-06-07 | 芝诺(苏州)生物科技有限公司 | 一种代谢工程改造毕赤酵母合成人乳铁蛋白的方法 |
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|---|---|---|---|---|
| US4546082A (en) | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US4601893A (en) | 1984-02-08 | 1986-07-22 | Pfizer Inc. | Laminate device for controlled and prolonged release of substances to an ambient environment and method of use |
| GB8726953D0 (en) | 1987-11-18 | 1987-12-23 | Delta Biotechnology Ltd | Yeast expression system |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5364780A (en) | 1989-03-17 | 1994-11-15 | E. I. Du Pont De Nemours And Company | External regulation of gene expression by inducible promoters |
| US5194600A (en) * | 1990-03-05 | 1993-03-16 | Royal Institute For The Advancement Of Learning | Genes which participate in β-glucan assembly and use thereof |
| DE4027453A1 (de) | 1990-08-30 | 1992-03-05 | Degussa | Neue plasmide aus corynebacterium glutamicum und davon abgeleitete plasmidvektoren |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| US6291205B1 (en) | 1992-06-12 | 2001-09-18 | Merck & Co., Inc. | Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae |
| EP0663010B1 (en) | 1992-10-02 | 2000-08-09 | Research Corporation Technologies, Inc. | Methods for increasing secretion of overexpressed proteins |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| AU3272695A (en) | 1994-08-12 | 1996-03-07 | Immunomedics Inc. | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
| US6068995A (en) * | 1994-10-25 | 2000-05-30 | Yoshitomi Pharmaceutical Industries, Ltd. | Method for producing protein |
| DE4440118C1 (de) | 1994-11-11 | 1995-11-09 | Forschungszentrum Juelich Gmbh | Die Genexpression in coryneformen Bakterien regulierende DNA |
| GB9515941D0 (en) | 1995-08-03 | 1995-10-04 | Zeneca Ltd | DNA constructs |
| GB9516241D0 (en) | 1995-08-08 | 1995-10-11 | Zeneca Ltd | Dna constructs |
| JPH10229891A (ja) | 1997-02-20 | 1998-09-02 | Mitsubishi Rayon Co Ltd | マロン酸誘導体の製造法 |
| FI972909A0 (fi) * | 1997-07-09 | 1997-07-09 | Valtion Teknillinen | Oekad produktion av avsoendrade proteiner genom rekombinatjaestceller |
| US7033781B1 (en) * | 1999-09-29 | 2006-04-25 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
| EP1266018B1 (en) | 2000-03-24 | 2008-05-07 | Genencor International, Inc. | Production of secreted proteins by recombinant eukaryotic cells |
| GB0104685D0 (en) * | 2001-02-26 | 2001-04-11 | Leuven K U Res & Dev | Tau-opathy model |
| WO2003012036A2 (en) | 2001-07-27 | 2003-02-13 | The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| SE526220C2 (sv) | 2003-12-23 | 2005-08-02 | Kvaerner Pulping Tech | Anordning samt förfarande för att mata ut cellulosamassa från ett cylindriskt lagringstorn |
| GB0329681D0 (en) | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Gene expression technique |
| WO2006067511A1 (en) | 2004-12-23 | 2006-06-29 | Novozymes Delta Limited | Gene expression technique |
| AU2005254734B9 (en) * | 2004-06-16 | 2011-10-13 | Dsm Ip Assets B.V. | Production of polypeptides by improved secretion |
| US7785872B2 (en) * | 2004-12-08 | 2010-08-31 | Simpson Biotech Co., Ltd. | Nucleic acids for enhancing gene expression and use thereof |
| AU2008241061A1 (en) | 2007-04-20 | 2008-10-30 | Polymun Scientific Immunbiologische Forschung Gmbh | Yeast expression systems |
| BRPI0816096A2 (pt) * | 2007-08-31 | 2016-03-22 | Basf Plant Science Gmbh | método para produzir uma célula de planta transgênica, uma planta ou uma parte da mesma com maior resistência a doença de planta, molécula de ácido nucleico isolado, construção de ácido nucleico, vetor, célula hospedeira, e, tecido de planta, construção de propagação, semente, material coletado ou uma planta. |
| US20090221030A1 (en) | 2007-11-01 | 2009-09-03 | Kai Bao | Signal sequences and co-expressed chaperones for improving protein production in a host cell |
| DE102008035152A1 (de) * | 2008-07-28 | 2010-02-04 | Evocatal Gmbh | Chaperon-Toolbox |
| WO2010135678A1 (en) * | 2009-05-22 | 2010-11-25 | Research Corporation Technologies, Inc. | Nucleic acids of pichia pastoris and use thereof for recombinant production of proteins |
| AU2011220878A1 (en) * | 2010-02-24 | 2012-08-23 | Merck Sharp & Dohme Corp. | Method for increasing N-glycosylation site occupancy on therapeutic glycoproteins produced in Pichia pastoris |
| CN102220254B (zh) * | 2010-04-14 | 2013-04-17 | 新疆农业科学院生物质能源研究所 | 一种重组酿酒酵母工程菌株及其应用 |
| JP6295512B2 (ja) | 2012-03-15 | 2018-03-20 | 株式会社豊田中央研究所 | 酵母における外来遺伝子の発現産物の生産方法、酵母における発現調節剤及びその利用 |
| KR101472273B1 (ko) * | 2012-03-29 | 2014-12-24 | 엘에스엠 주식회사 | ＣＧＴａｓｅ의 세포 표면 발현 재조합 벡터 |
| SG10201809152QA (en) * | 2014-04-17 | 2018-11-29 | Boehringer Ingelheim Rcv Gmbh | Recombinant host cell for expressing proteins of interest |
-
2015
- 2015-04-16 KR KR1020167032074A patent/KR102373954B1/ko active Active
- 2015-04-16 SG SG11201608084TA patent/SG11201608084TA/en unknown
- 2015-04-16 JP JP2016562915A patent/JP2017511148A/ja active Pending
- 2015-04-16 CA CA2948369A patent/CA2948369C/en active Active
- 2015-04-16 US US15/304,050 patent/US10865416B2/en active Active
- 2015-04-16 WO PCT/EP2015/058242 patent/WO2015158808A2/en not_active Ceased
- 2015-04-16 EP EP15721134.3A patent/EP3132028B1/en active Active
- 2015-04-16 SG SG10201809146QA patent/SG10201809146QA/en unknown
- 2015-04-16 AU AU2015248815A patent/AU2015248815B2/en active Active
- 2015-04-16 EP EP23205278.7A patent/EP4311857A3/en active Pending
- 2015-04-16 BR BR112016023304A patent/BR112016023304A2/pt not_active Application Discontinuation
- 2015-04-16 CN CN201580032989.7A patent/CN106661541B/zh active Active
-
2016
- 2016-10-18 IL IL248376A patent/IL248376B/en active IP Right Grant
-
2019
- 2019-12-26 JP JP2019236345A patent/JP7062633B2/ja active Active
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