JP2020509751A5 - - Google Patents
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- Publication number
- JP2020509751A5 JP2020509751A5 JP2019548882A JP2019548882A JP2020509751A5 JP 2020509751 A5 JP2020509751 A5 JP 2020509751A5 JP 2019548882 A JP2019548882 A JP 2019548882A JP 2019548882 A JP2019548882 A JP 2019548882A JP 2020509751 A5 JP2020509751 A5 JP 2020509751A5
- Authority
- JP
- Japan
- Prior art keywords
- expression construct
- recombinant
- recombinant host
- protein
- host cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 230000014509 gene expression Effects 0.000 claims description 41
- 210000004027 cell Anatomy 0.000 claims description 26
- 108090000623 proteins and genes Proteins 0.000 claims description 21
- 102000004169 proteins and genes Human genes 0.000 claims description 21
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 14
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 12
- 102000040430 polynucleotide Human genes 0.000 claims description 12
- 108091033319 polynucleotide Proteins 0.000 claims description 12
- 239000002157 polynucleotide Substances 0.000 claims description 12
- 230000003248 secreting effect Effects 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 8
- 210000005253 yeast cell Anatomy 0.000 claims description 6
- 241000235058 Komagataella pastoris Species 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 3
- 241000235648 Pichia Species 0.000 claims description 2
- 102000040739 Secretory proteins Human genes 0.000 claims description 2
- 108091058545 Secretory proteins Proteins 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 230000028327 secretion Effects 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical group C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 241000235343 Saccharomycetales Species 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762470144P | 2017-03-10 | 2017-03-10 | |
| US62/470,144 | 2017-03-10 | ||
| PCT/US2018/021812 WO2018165589A2 (en) | 2017-03-10 | 2018-03-09 | Compositions and methods for producing high secreted yields of recombinant proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020509751A JP2020509751A (ja) | 2020-04-02 |
| JP2020509751A5 true JP2020509751A5 (enExample) | 2021-04-08 |
| JP7237365B2 JP7237365B2 (ja) | 2023-03-13 |
Family
ID=63448293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019548882A Active JP7237365B2 (ja) | 2017-03-10 | 2018-03-09 | 高分泌収量の組み換えタンパク質を産生するための組成物及び方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US11306127B2 (enExample) |
| EP (1) | EP3592800A4 (enExample) |
| JP (1) | JP7237365B2 (enExample) |
| KR (1) | KR102638074B1 (enExample) |
| WO (1) | WO2018165589A2 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3263593A1 (en) * | 2016-07-01 | 2018-01-03 | Anna Rising | Engineered spider silk proteins and uses thereof |
| WO2021106017A1 (en) * | 2019-11-27 | 2021-06-03 | Revelations Biotech Pvt Ltd | Nucleic acids, vectors, host cells and methods for production of fructosyltransferase from aspergillus japonicus |
| CN111500479B (zh) * | 2020-04-29 | 2022-12-27 | 江南大学 | 一种非甲醇诱导双启动子毕赤酵母工程菌的构建及其应用 |
| IL304910A (en) | 2021-02-12 | 2023-10-01 | Boehringer Ingelheim Rcv Gmbh | Signal peptides for increased protein secretion |
| JP7756787B2 (ja) * | 2021-07-30 | 2025-10-20 | ヘライナ，インコーポレーテッド | タンパク質合成および分泌のための方法および組成物 |
| CN115960175A (zh) * | 2022-08-08 | 2023-04-14 | 江南大学 | 一种强化乳铁蛋白表达的毕赤酵母及其构建方法与应用 |
| AU2023343332A1 (en) * | 2022-09-13 | 2025-04-03 | Ginkgo Bioworks, Inc. | Production of proteins, including secreted proteins |
| KR20240071112A (ko) | 2022-11-15 | 2024-05-22 | 숙명여자대학교산학협력단 | 사료용 미생물단백질 생산을 위한 신규한 사카로마이세스 세레비지에 균주 |
| EP4642916A2 (en) * | 2022-12-30 | 2025-11-05 | Biotalys NV | Secretion signals |
| WO2025059777A1 (en) * | 2023-09-22 | 2025-03-27 | Biofect Innovations Inc. | Production and secretion of recombinant proteins in yeast |
| KR20250082126A (ko) | 2023-11-29 | 2025-06-09 | 인하대학교 산학협력단 | Nad+를 포함하는 세포 배양용 배지 조성물 및 이의 용도 |
| CN119464103B (zh) * | 2025-01-17 | 2025-05-09 | 上海昌进生物科技有限公司 | 高效表达β-乳球蛋白的菌株及其构建方法和应用 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6100042A (en) * | 1993-03-31 | 2000-08-08 | Cadus Pharmaceutical Corporation | Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor |
| US20030013154A1 (en) * | 1998-02-24 | 2003-01-16 | Chiron Corporation | Pichia secretory leader for protein expression |
| WO2001077351A1 (en) * | 2000-04-07 | 2001-10-18 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Vectors and methods for dual protein expression in pichia pastoris and escherichia coli |
| JP2003135058A (ja) * | 2001-08-21 | 2003-05-13 | Ajinomoto Co Inc | メタノール資化性酵母を用いたトランスグルタミナーゼの製造法 |
| KR20030062854A (ko) * | 2002-01-21 | 2003-07-28 | 주식회사 엘지생명과학 | 분비형 벡터를 이용한 효모에서의 재조합 단백질의 제조방법 |
| US7314974B2 (en) | 2002-02-21 | 2008-01-01 | Monsanto Technology, Llc | Expression of microbial proteins in plants for production of plants with improved properties |
| JP2006501166A (ja) | 2002-06-11 | 2006-01-12 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | 異種前立腺タンパク質ｐ５０１ｓを含む免疫原性組成物 |
| EP1711529A1 (en) | 2004-01-21 | 2006-10-18 | Novozymes A/S | Production of a monoclonal antibody in a heterokaryon fungus or in a fungal host cell |
| US20070248967A1 (en) * | 2005-06-09 | 2007-10-25 | National Institute Of Advanced Industrial Science | Reporter Assay Using Secrectory Luminescent Enzymes |
| KR20070009269A (ko) | 2005-07-15 | 2007-01-18 | 한국생명공학연구원 | 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자 |
| JP5354559B2 (ja) * | 2005-11-24 | 2013-11-27 | 独立行政法人産業技術総合研究所 | 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系 |
| WO2008052043A2 (en) * | 2006-10-24 | 2008-05-02 | Cogenesys, Inc. | Opioid receptor agonist fusion proteins |
| US20110165681A1 (en) * | 2009-02-26 | 2011-07-07 | Massachusetts Institute Of Technology | Light-Activated Proton Pumps and Applications Thereof |
| WO2010058057A1 (es) * | 2008-11-21 | 2010-05-27 | Consejo Superior De Investigaciones Científicas (Csic) (90%) | Lacasas de alto potencial redox obtenidas por evolución dirigida |
| EP2258855A1 (en) | 2009-05-28 | 2010-12-08 | Universität für Bodenkultur Wien | Expression sequences |
| US8785159B2 (en) | 2009-11-25 | 2014-07-22 | Alliance For Sustainable Energy, Llc | Extracellular secretion of recombinant proteins |
| GB201105418D0 (en) * | 2011-03-31 | 2011-05-18 | Univ Durham | Pesticide |
| US9732146B2 (en) * | 2012-03-30 | 2017-08-15 | The United States Of America As Represented By The Department Of Veterans Affairs | Antibody-mediated transduction of heat shock proteins into living cells |
| US20150293076A1 (en) | 2012-10-22 | 2015-10-15 | Bolt Threads, Inc. | Cellular Reprogramming for Product Optimization |
| TWI636136B (zh) | 2013-03-15 | 2018-09-21 | 艾爾德生物製藥股份有限公司 | 在酵母菌及其它轉型細胞中多肽高產量表現用之溫度變動技術 |
| CA2924343C (en) * | 2013-09-17 | 2025-09-16 | Bolt Threads, Inc. | PROCESSES AND COMPOSITIONS FOR SYNTHESIZING IMPROVED SILK FIBERS |
| CN114365792A (zh) * | 2014-11-11 | 2022-04-19 | 克莱拉食品公司 | 用于生成卵清蛋白的方法和组合物 |
| WO2016149414A1 (en) | 2015-03-16 | 2016-09-22 | Bolt Threads, Inc. | Improved silk fibers |
| EP3307765B1 (en) | 2015-06-11 | 2024-04-10 | Bolt Threads, Inc. | Recombinant protein fiber yarns with improved properties |
| US20200399646A9 (en) * | 2017-01-10 | 2020-12-24 | Massachusetts Institute Of Technology | Constructs and cells for enhanced protein expression |
| WO2018165594A1 (en) | 2017-03-10 | 2018-09-13 | Bolt Threads, Inc. | Compositions and methods for producing high secreted yields of recombinant proteins |
| US20240026331A1 (en) * | 2022-05-10 | 2024-01-25 | Codexis, Inc. | Recombinant neprosin polypeptides and methods of expression |
-
2018
- 2018-03-09 JP JP2019548882A patent/JP7237365B2/ja active Active
- 2018-03-09 KR KR1020197029626A patent/KR102638074B1/ko active Active
- 2018-03-09 WO PCT/US2018/021812 patent/WO2018165589A2/en not_active Ceased
- 2018-03-09 EP EP18764854.8A patent/EP3592800A4/en active Pending
- 2018-03-13 US US15/920,291 patent/US11306127B2/en active Active
-
2022
- 2022-03-15 US US17/695,219 patent/US11725030B2/en active Active
-
2023
- 2023-06-26 US US18/341,669 patent/US12325730B2/en active Active
-
2025
- 2025-05-30 US US19/223,644 patent/US20250289856A1/en active Pending
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