JP2020509751A5 - - Google Patents

Info

Publication number

JP2020509751A5

JP2020509751A5 JP2019548882A JP2019548882A JP2020509751A5 JP 2020509751 A5 JP2020509751 A5 JP 2020509751A5 JP 2019548882 A JP2019548882 A JP 2019548882A JP 2019548882 A JP2019548882 A JP 2019548882A JP 2020509751 A5 JP2020509751 A5 JP 2020509751A5

Authority

JP

Japan

Prior art keywords

expression construct

recombinant

recombinant host

protein

host cell

Prior art date

2018-03-09

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 230000014509 gene expression Effects 0.000 claims description 41
• 210000004027 cell Anatomy 0.000 claims description 26
• 108090000623 proteins and genes Proteins 0.000 claims description 21
• 102000004169 proteins and genes Human genes 0.000 claims description 21
• 108010076504 Protein Sorting Signals Proteins 0.000 claims description 14
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 12
• 102000040430 polynucleotide Human genes 0.000 claims description 12
• 108091033319 polynucleotide Proteins 0.000 claims description 12
• 239000002157 polynucleotide Substances 0.000 claims description 12
• 230000003248 secreting effect Effects 0.000 claims description 11
• 238000000855 fermentation Methods 0.000 claims description 10
• 230000004151 fermentation Effects 0.000 claims description 10
• 239000001963 growth medium Substances 0.000 claims description 8
• 210000005253 yeast cell Anatomy 0.000 claims description 6
• 241000235058 Komagataella pastoris Species 0.000 claims description 5
• 238000000034 method Methods 0.000 claims description 3
• 241000235648 Pichia Species 0.000 claims description 2
• 102000040739 Secretory proteins Human genes 0.000 claims description 2
• 108091058545 Secretory proteins Proteins 0.000 claims description 2
• 150000001413 amino acids Chemical class 0.000 claims description 2
• 238000012258 culturing Methods 0.000 claims description 2
• 230000001939 inductive effect Effects 0.000 claims description 2
• 238000004519 manufacturing process Methods 0.000 claims description 2
• 230000028327 secretion Effects 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical group C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
• 241000235343 Saccharomycetales Species 0.000 description 1
• 230000000694 effects Effects 0.000 description 1
• 230000000116 mitigating effect Effects 0.000 description 1
• 230000002018 overexpression Effects 0.000 description 1