KR102638074B1 - 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 - Google Patents

재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 Download PDF

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KR102638074B1
KR102638074B1 KR1020197029626A KR20197029626A KR102638074B1 KR 102638074 B1 KR102638074 B1 KR 102638074B1 KR 1020197029626 A KR1020197029626 A KR 1020197029626A KR 20197029626 A KR20197029626 A KR 20197029626A KR 102638074 B1 KR102638074 B1 KR 102638074B1
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KR20190127802A (ko
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조슈아 키틀슨
토마스 스티븐슨
레나 힐
카를로스 구스타보 페세
데이비드 브레슬라우
다니엘 위드마이어
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볼트 쓰레즈, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Insects & Arthropods (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020197029626A 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 Active KR102638074B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762470144P 2017-03-10 2017-03-10
US62/470,144 2017-03-10
PCT/US2018/021812 WO2018165589A2 (en) 2017-03-10 2018-03-09 Compositions and methods for producing high secreted yields of recombinant proteins

Publications (2)

Publication Number Publication Date
KR20190127802A KR20190127802A (ko) 2019-11-13
KR102638074B1 true KR102638074B1 (ko) 2024-02-20

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US (4) US11306127B2 (enExample)
EP (1) EP3592800A4 (enExample)
JP (1) JP7237365B2 (enExample)
KR (1) KR102638074B1 (enExample)
WO (1) WO2018165589A2 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3263593A1 (en) * 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
BR112022008699A2 (pt) * 2019-11-27 2022-07-26 Revelations Biotech Pvt Ltd Ácidos nucleicos, vetores, células hospedeiras e métodos para a produção de frutosiltransferase a partir de aspergillus japonicus
CN111500479B (zh) * 2020-04-29 2022-12-27 江南大学 一种非甲醇诱导双启动子毕赤酵母工程菌的构建及其应用
CN117120618A (zh) 2021-02-12 2023-11-24 勃林格殷格翰 Rcv 两合公司 用于增加蛋白质分泌的信号肽
CA3187918A1 (en) * 2021-07-30 2023-01-30 Helaina, Inc. Methods and compositions for protein synthesis and secretion
CN115960175A (zh) * 2022-08-08 2023-04-14 江南大学 一种强化乳铁蛋白表达的毕赤酵母及其构建方法与应用
EP4587574A1 (en) * 2022-09-13 2025-07-23 Ginkgo Bioworks, Inc. Production of proteins, including secreted proteins
KR20240071112A (ko) 2022-11-15 2024-05-22 숙명여자대학교산학협력단 사료용 미생물단백질 생산을 위한 신규한 사카로마이세스 세레비지에 균주
EP4642916A2 (en) 2022-12-30 2025-11-05 Biotalys NV Secretion signals
WO2025059777A1 (en) * 2023-09-22 2025-03-27 Biofect Innovations Inc. Production and secretion of recombinant proteins in yeast
KR20250082126A (ko) 2023-11-29 2025-06-09 인하대학교 산학협력단 Nad+를 포함하는 세포 배양용 배지 조성물 및 이의 용도
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
CN119464103B (zh) * 2025-01-17 2025-05-09 上海昌进生物科技有限公司 高效表达β-乳球蛋白的菌株及其构建方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016077457A1 (en) * 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
US20160222174A1 (en) * 2013-09-17 2016-08-04 Bolt Threads, Inc. Method and Compositions for Synthesizing Improved Silk Fibers

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6100042A (en) * 1993-03-31 2000-08-08 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US20030013154A1 (en) * 1998-02-24 2003-01-16 Chiron Corporation Pichia secretory leader for protein expression
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
JP2003135058A (ja) * 2001-08-21 2003-05-13 Ajinomoto Co Inc メタノール資化性酵母を用いたトランスグルタミナーゼの製造法
KR20030062854A (ko) * 2002-01-21 2003-07-28 주식회사 엘지생명과학 분비형 벡터를 이용한 효모에서의 재조합 단백질의 제조방법
US7314974B2 (en) 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
CA2487907A1 (en) 2002-06-11 2003-12-18 Glaxosmithkline Biologicals S.A. Immunogenic compositions comprising a xenogenic prostate protein p501s
WO2005070962A1 (en) 2004-01-21 2005-08-04 Novozymes A/S Production of a monoclonal antibody in a heterokaryon fungus or in a fungal host cell
US20070248967A1 (en) * 2005-06-09 2007-10-25 National Institute Of Advanced Industrial Science Reporter Assay Using Secrectory Luminescent Enzymes
KR20070009269A (ko) 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
JP5354559B2 (ja) * 2005-11-24 2013-11-27 独立行政法人産業技術総合研究所 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
WO2008052043A2 (en) * 2006-10-24 2008-05-02 Cogenesys, Inc. Opioid receptor agonist fusion proteins
US20110165681A1 (en) * 2009-02-26 2011-07-07 Massachusetts Institute Of Technology Light-Activated Proton Pumps and Applications Thereof
WO2010058057A1 (es) * 2008-11-21 2010-05-27 Consejo Superior De Investigaciones Científicas (Csic) (90%) Lacasas de alto potencial redox obtenidas por evolución dirigida
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
US8785159B2 (en) 2009-11-25 2014-07-22 Alliance For Sustainable Energy, Llc Extracellular secretion of recombinant proteins
GB201105418D0 (en) * 2011-03-31 2011-05-18 Univ Durham Pesticide
US9732146B2 (en) * 2012-03-30 2017-08-15 The United States Of America As Represented By The Department Of Veterans Affairs Antibody-mediated transduction of heat shock proteins into living cells
US20150293076A1 (en) 2012-10-22 2015-10-15 Bolt Threads, Inc. Cellular Reprogramming for Product Optimization
ES3057435T3 (en) 2013-03-15 2026-03-02 H Lundbeck As Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
CN114000218A (zh) 2015-03-16 2022-02-01 保尔特纺织品公司 改善的丝纤维
JP2018521239A (ja) 2015-06-11 2018-08-02 ボルト スレッズ インコーポレイテッド 改善された特性を有する組換えタンパク質繊維糸
WO2018132512A1 (en) * 2017-01-10 2018-07-19 Massachusetts Institute Of Technology Constructs and cells for enhanced protein expression
KR20250121449A (ko) 2017-03-10 2025-08-12 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
WO2023220658A2 (en) * 2022-05-10 2023-11-16 Codexis, Inc. Recombinant neprosin polypeptides and methods of expression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160222174A1 (en) * 2013-09-17 2016-08-04 Bolt Threads, Inc. Method and Compositions for Synthesizing Improved Silk Fibers
WO2016077457A1 (en) * 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production

Also Published As

Publication number Publication date
JP7237365B2 (ja) 2023-03-13
WO2018165589A2 (en) 2018-09-13
KR20190127802A (ko) 2019-11-13
US20220315630A1 (en) 2022-10-06
US11306127B2 (en) 2022-04-19
US20250289856A1 (en) 2025-09-18
JP2020509751A (ja) 2020-04-02
WO2018165589A3 (en) 2018-10-18
US20240150416A1 (en) 2024-05-09
US20180282380A1 (en) 2018-10-04
EP3592800A2 (en) 2020-01-15
US11725030B2 (en) 2023-08-15
EP3592800A4 (en) 2021-01-06
US12325730B2 (en) 2025-06-10

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