MX366647B - Cambio de temperatura para una expresión con mayor rendimiento de polipéptidos en levaduras y otras células transformadas. - Google Patents
Cambio de temperatura para una expresión con mayor rendimiento de polipéptidos en levaduras y otras células transformadas.Info
- Publication number
- MX366647B MX366647B MX2015011968A MX2015011968A MX366647B MX 366647 B MX366647 B MX 366647B MX 2015011968 A MX2015011968 A MX 2015011968A MX 2015011968 A MX2015011968 A MX 2015011968A MX 366647 B MX366647 B MX 366647B
- Authority
- MX
- Mexico
- Prior art keywords
- yeast
- transformed cells
- polypeptides
- high yield
- temperature shift
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/244—Interleukins [IL]
- C07K16/248—IL-6
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Analytical Chemistry (AREA)
Abstract
La presente invención se refiere a métodos para producir proteínas heterólogas. En particular, la presente divulgación provee métodos mejorados para producir proteínas deseadas, que incluyen proteínas con múltiples subunidades tal como anticuerpos, con un rendimiento mayor y pureza mejorada. En formas de realización ejemplificativas, las células transformadas son una levadura, por ejemplo, levadura metilotrófica tal como Pichia pastoris. Un método para producir un complejo recombinante de múltiples subunidades en células de levadura, caracterizado porque comprende: (a) cultivar a una primera temperatura las células de lavadura que comprenden uno o más genes que codifican el complejo de múltiples subunidades; y (b) cultivar las células de levadura a una segunda temperatura y permitir que las células de levadura produzcan el complejo de múltiples subunidades.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361791471P | 2013-03-15 | 2013-03-15 | |
| US201361790613P | 2013-03-15 | 2013-03-15 | |
| PCT/US2014/030453 WO2014145650A1 (en) | 2013-03-15 | 2014-03-17 | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2015011968A MX2015011968A (es) | 2017-01-06 |
| MX366647B true MX366647B (es) | 2019-07-17 |
Family
ID=51538058
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015011968A MX366647B (es) | 2013-03-15 | 2014-03-17 | Cambio de temperatura para una expresión con mayor rendimiento de polipéptidos en levaduras y otras células transformadas. |
| MX2019008417A MX384491B (es) | 2013-03-15 | 2014-03-17 | Cambio de temperatura para una expresion con mayor rendimiento de polipeptidos en levaduras y otras celulas transformadas. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019008417A MX384491B (es) | 2013-03-15 | 2014-03-17 | Cambio de temperatura para una expresion con mayor rendimiento de polipeptidos en levaduras y otras celulas transformadas. |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US10138294B2 (es) |
| EP (2) | EP3597664B1 (es) |
| JP (1) | JP6412552B2 (es) |
| KR (1) | KR102202477B1 (es) |
| CN (1) | CN105121652B (es) |
| AU (1) | AU2014232813B2 (es) |
| CA (1) | CA2905180C (es) |
| DK (1) | DK2971037T3 (es) |
| ES (2) | ES2749751T3 (es) |
| IL (1) | IL241176B (es) |
| MX (2) | MX366647B (es) |
| SG (1) | SG11201506034TA (es) |
| TW (1) | TWI636136B (es) |
| WO (1) | WO2014145650A1 (es) |
| ZA (1) | ZA201505483B (es) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10202630B2 (en) * | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| MX378344B (es) | 2014-03-21 | 2025-03-10 | Teva Pharmaceuticals Int Gmbh | Anticuerpos antagonistas dirigidos contra el peptido relacionado con el gen de calcitonina y metodos que usan los mismos. |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| WO2016077457A1 (en) | 2014-11-11 | 2016-05-19 | Clara Foods Co. | Methods and compositions for egg white protein production |
| JP2017045865A (ja) | 2015-08-26 | 2017-03-02 | ルネサスエレクトロニクス株式会社 | 半導体装置およびその製造方法 |
| CN106191181B (zh) | 2016-07-22 | 2019-12-13 | 江苏江山聚源生物技术有限公司 | 一种毕赤酵母表达重组蛋白的发酵工艺 |
| SMT202600087T1 (it) | 2016-09-23 | 2026-03-09 | Teva Pharmaceuticals Int Gmbh | Trattamento dell’emicrania refrattaria |
| KR20250121449A (ko) | 2017-03-10 | 2025-08-12 | 볼트 쓰레즈, 인크. | 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 |
| WO2018165589A2 (en) | 2017-03-10 | 2018-09-13 | Bolt Threads, Inc. | Compositions and methods for producing high secreted yields of recombinant proteins |
| MA52007A (fr) * | 2018-03-16 | 2021-01-20 | Genzyme Corp | Procédés d'amélioration de la viabilité cellulaire dans un bioréacteur de production |
| US10221403B1 (en) * | 2018-04-25 | 2019-03-05 | Life Rainbow Biotech Co., Ltd. | Method of preparing zearalenone hydrolase |
| CN108546637A (zh) * | 2018-05-23 | 2018-09-18 | 华东理工大学 | 重组毕赤酵母表达葡萄糖氧化酶发酵装置以及发酵培养方法 |
| CN112703251A (zh) * | 2018-07-12 | 2021-04-23 | 阿迈瑞斯公司 | 控制发酵进料速率的方法 |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| FI13395Y1 (fi) | 2019-07-11 | 2023-06-06 | Clara Foods Co | Koostumuksia, jotka käsittävät rekombinanttia ovomukoidiproteiinia |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| EP4055177B1 (en) * | 2019-11-08 | 2024-07-31 | Danisco US Inc. | Fungal strains comprising enhanced protein productivity phenotypes and methods thereof |
| WO2021146233A1 (en) | 2020-01-14 | 2021-07-22 | Basf Se | Method for producing recombinant protein in yeast cells |
| US20250207158A1 (en) * | 2022-03-24 | 2025-06-26 | Versalis S.P.A. | Fermentation process for the production of lipids from oleaginous yeasts |
| US20260103736A1 (en) * | 2022-09-29 | 2026-04-16 | Antheia, Inc. | Methods Of Improving Production Of Morphinan Alkaloids And Derivatives |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| AU6445894A (en) | 1993-03-19 | 1994-10-11 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| AU3272695A (en) | 1994-08-12 | 1996-03-07 | Immunomedics Inc. | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6221630B1 (en) * | 1999-03-24 | 2001-04-24 | The Penn State Research Foundation | High copy number recombinant expression construct for regulated high-level production of polypeptides in yeast |
| DK1678314T3 (da) | 2003-10-22 | 2012-12-03 | Keck Graduate Inst | Fremgangsmåde til syntetisering af heteromultimere polypeptider i gær ved anvendelse af en haploid parringsstrategi |
| EP2021463B1 (en) | 2006-05-19 | 2016-11-23 | Alder Biopharmaceuticals, Inc. | Culture method for obtaining a clonal population of antigen-specific b cells |
| DK2164514T3 (en) | 2007-05-21 | 2017-02-27 | Alderbio Holdings Llc | Antibodies to IL-6 and its use |
| NZ601583A (en) | 2007-05-21 | 2013-08-30 | Bristol Myers Squibb Co | Novel rabbit antibody humanization methods and humanized rabbit antibodies |
| US20090269832A1 (en) | 2007-11-05 | 2009-10-29 | Modular Genetics, Inc. | Growth of Microorganisms in Media Containing Crude Glycerol |
| US8501449B2 (en) * | 2007-12-04 | 2013-08-06 | Proteon Therapeutics, Inc. | Recombinant elastase proteins and methods of manufacturing and use thereof |
| US8609416B2 (en) | 2009-12-18 | 2013-12-17 | Ventria Bioscience | Methods and compositions comprising heat shock proteins |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| SG2014011878A (en) * | 2011-08-19 | 2014-04-28 | Alderbio Holdings Llc | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
| US10150968B2 (en) | 2011-08-19 | 2018-12-11 | Alderbio Holdings Llc | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| US10202630B2 (en) * | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| WO2014145521A2 (en) * | 2013-03-15 | 2014-09-18 | Alderbio Holdings Llc | Fermentation process for antibody production |
-
2014
- 2014-03-17 TW TW103109931A patent/TWI636136B/zh active
- 2014-03-17 ES ES14764393T patent/ES2749751T3/es active Active
- 2014-03-17 WO PCT/US2014/030453 patent/WO2014145650A1/en not_active Ceased
- 2014-03-17 DK DK14764393.6T patent/DK2971037T3/da active
- 2014-03-17 EP EP19177519.6A patent/EP3597664B1/en active Active
- 2014-03-17 MX MX2015011968A patent/MX366647B/es active IP Right Grant
- 2014-03-17 ES ES19177519T patent/ES3057435T3/es active Active
- 2014-03-17 JP JP2016503404A patent/JP6412552B2/ja active Active
- 2014-03-17 US US14/776,892 patent/US10138294B2/en active Active
- 2014-03-17 CA CA2905180A patent/CA2905180C/en active Active
- 2014-03-17 CN CN201480014647.8A patent/CN105121652B/zh active Active
- 2014-03-17 EP EP14764393.6A patent/EP2971037B1/en active Active
- 2014-03-17 KR KR1020157028401A patent/KR102202477B1/ko active Active
- 2014-03-17 MX MX2019008417A patent/MX384491B/es unknown
- 2014-03-17 AU AU2014232813A patent/AU2014232813B2/en active Active
- 2014-03-17 SG SG11201506034TA patent/SG11201506034TA/en unknown
-
2015
- 2015-07-30 ZA ZA2015/05483A patent/ZA201505483B/en unknown
- 2015-09-03 IL IL241176A patent/IL241176B/en active IP Right Grant
-
2018
- 2018-10-24 US US16/169,130 patent/US11447540B2/en active Active
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Legal Events
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| FG | Grant or registration |