KR102373954B1 - 보조 단백질을 과발현하도록 조작된 재조합 숙주세포 - Google Patents

보조 단백질을 과발현하도록 조작된 재조합 숙주세포 Download PDF

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KR102373954B1
KR102373954B1 KR1020167032074A KR20167032074A KR102373954B1 KR 102373954 B1 KR102373954 B1 KR 102373954B1 KR 1020167032074 A KR1020167032074 A KR 1020167032074A KR 20167032074 A KR20167032074 A KR 20167032074A KR 102373954 B1 KR102373954 B1 KR 102373954B1
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protein
host cell
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KR20170002453A (ko
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브리기테 가세르
디트하르트 마타노비흐
마르쿠스 부셰틱스
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베링거 인겔하임 에르체파우 게엠베하 운트 코 카게
발리도겐 게엠베하
론자 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020167032074A 2014-04-17 2015-04-16 보조 단백질을 과발현하도록 조작된 재조합 숙주세포 Active KR102373954B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14165186 2014-04-17
EP14165186.9 2014-04-17
PCT/EP2015/058242 WO2015158808A2 (en) 2014-04-17 2015-04-16 Recombinant host cell engineered to overexpress helper proteins

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KR20170002453A KR20170002453A (ko) 2017-01-06
KR102373954B1 true KR102373954B1 (ko) 2022-03-14

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US (1) US10865416B2 (enExample)
EP (2) EP4311857A3 (enExample)
JP (2) JP2017511148A (enExample)
KR (1) KR102373954B1 (enExample)
CN (1) CN106661541B (enExample)
AU (1) AU2015248815B2 (enExample)
BR (1) BR112016023304A2 (enExample)
CA (1) CA2948369C (enExample)
IL (1) IL248376B (enExample)
SG (2) SG11201608084TA (enExample)
WO (1) WO2015158808A2 (enExample)

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WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
SG10202110774QA (en) * 2017-03-29 2021-11-29 Boehringer Ingelheim Rcv Gmbh Recombinant host cell with altered membrane lipid composition
CN109722445A (zh) * 2017-10-27 2019-05-07 成都必高生物科技有限公司 提升溶菌酶产量及活性的方法
KR20200086303A (ko) * 2017-11-20 2020-07-16 아젠스 홀딩 비.브이. 숙주 세포에서의 향미 화합물 생산
JP7623680B2 (ja) * 2018-05-17 2025-01-29 ボルト スレッズ インコーポレイテッド 組換えタンパク質の分泌を改善するためのｓｅｃ改変株
CA3103988A1 (en) * 2018-06-27 2020-01-02 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for increased protein expression by use of transcription factors
CN112739819A (zh) * 2018-09-24 2021-04-30 中东科技大学 利用启动子工程化设计醇脱氢酶2(adh2)启动子变体
CN109439640B (zh) * 2018-10-09 2021-07-20 天津科技大学 伏马毒素降解酶FumDSB及其基因和应用
CN109439639B (zh) * 2018-10-09 2021-07-20 天津科技大学 伏马毒素降解酶FumDPS及其基因和应用
CN111378585B (zh) 2018-12-28 2023-06-16 丰益(上海)生物技术研发中心有限公司 用于表达外源基因的毕赤酵母突变株
DK3874047T4 (da) 2019-01-11 2025-02-03 Lonza Ag Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle
DK3997118T3 (da) 2019-07-11 2025-03-24 Clara Foods Co Drikkevaresammensætning, der indeholder rekombinant ovomucoid-protein
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
AU2021216950A1 (en) * 2020-02-04 2022-09-01 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof
CN111349177B (zh) * 2020-03-13 2022-04-22 西北农林科技大学 一种融合抗菌肽cat的制备方法和应用
CN111549007B (zh) * 2020-04-07 2022-10-04 天津科技大学 一种转氨酶tsta、制备方法和应用
CN111607575B (zh) * 2020-04-07 2022-10-04 天津科技大学 一种转氨酶phta、制备方法和应用
CN113088533B (zh) * 2021-04-15 2023-03-24 华中科技大学 一种高效表达藤壶粘胶蛋白的酵母工程菌及其制备方法
CN113801801B (zh) * 2021-10-08 2023-03-07 枣庄市杰诺生物酶有限公司 一株高效生产碱性果胶酶的重组菌及其应用
CN118147180A (zh) * 2024-05-09 2024-06-07 芝诺(苏州)生物科技有限公司 一种代谢工程改造毕赤酵母合成人乳铁蛋白的方法

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Publication number Publication date
WO2015158808A2 (en) 2015-10-22
EP4311857A2 (en) 2024-01-31
KR20170002453A (ko) 2017-01-06
CN106661541A (zh) 2017-05-10
WO2015158808A3 (en) 2016-01-28
JP7062633B2 (ja) 2022-05-06
US10865416B2 (en) 2020-12-15
IL248376A0 (en) 2016-11-30
EP3132028A2 (en) 2017-02-22
AU2015248815A1 (en) 2016-11-03
SG11201608084TA (en) 2016-10-28
SG10201809146QA (en) 2018-11-29
CA2948369C (en) 2023-09-26
IL248376B (en) 2021-04-29
EP4311857A3 (en) 2024-07-31
EP3132028B1 (en) 2023-10-25
US20170029827A1 (en) 2017-02-02
JP2020072696A (ja) 2020-05-14
BR112016023304A2 (pt) 2017-10-17
CN106661541B (zh) 2021-09-03
JP2017511148A (ja) 2017-04-20
AU2015248815B2 (en) 2021-07-08
CA2948369A1 (en) 2015-10-22

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