AU2015248815B2 - Recombinant host cell engineered to overexpress helper proteins - Google Patents

Recombinant host cell engineered to overexpress helper proteins Download PDF

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Publication number
AU2015248815B2
AU2015248815B2 AU2015248815A AU2015248815A AU2015248815B2 AU 2015248815 B2 AU2015248815 B2 AU 2015248815B2 AU 2015248815 A AU2015248815 A AU 2015248815A AU 2015248815 A AU2015248815 A AU 2015248815A AU 2015248815 B2 AU2015248815 B2 AU 2015248815B2
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Australia
Prior art keywords
protein
seq
host cell
functional homologue
amino acid
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AU2015248815A
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AU2015248815A1 (en
Inventor
Markus BUCHETICS
Brigitte Gasser
Diethard Mattanovich
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Boehringer Ingelheim RCV GmbH and Co KG
Validogen GmbH
Lonza AG
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Boehringer Ingelheim RCV GmbH and Co KG
Validogen GmbH
Lonza AG
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Assigned to SANDOZ AG, LONZA LTD., BOEHRINGER INGELHEIM RCV GMBH & CO KG, VALIDOGEN GMBH reassignment SANDOZ AG Amend patent request/document other than specification (104) Assignors: BOEHRINGER INGELHEIM RCV GMBH & CO KG, LONZA LTD., SANDOZ AG, VTU TECHNOLOGY GMBH
Assigned to LONZA LTD., BOEHRINGER INGELHEIM RCV GMBH & CO KG, VALIDOGEN GMBH reassignment LONZA LTD. Request for Assignment Assignors: BOEHRINGER INGELHEIM RCV GMBH & CO KG, LONZA LTD., SANDOZ AG, VALIDOGEN GMBH
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2015248815A 2014-04-17 2015-04-16 Recombinant host cell engineered to overexpress helper proteins Active AU2015248815B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14165186 2014-04-17
EP14165186.9 2014-04-17
PCT/EP2015/058242 WO2015158808A2 (en) 2014-04-17 2015-04-16 Recombinant host cell engineered to overexpress helper proteins

Publications (2)

Publication Number Publication Date
AU2015248815A1 AU2015248815A1 (en) 2016-11-03
AU2015248815B2 true AU2015248815B2 (en) 2021-07-08

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AU2015248815A Active AU2015248815B2 (en) 2014-04-17 2015-04-16 Recombinant host cell engineered to overexpress helper proteins

Country Status (11)

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US (1) US10865416B2 (enExample)
EP (2) EP4311857A3 (enExample)
JP (2) JP2017511148A (enExample)
KR (1) KR102373954B1 (enExample)
CN (1) CN106661541B (enExample)
AU (1) AU2015248815B2 (enExample)
BR (1) BR112016023304A2 (enExample)
CA (1) CA2948369C (enExample)
IL (1) IL248376B (enExample)
SG (2) SG11201608084TA (enExample)
WO (1) WO2015158808A2 (enExample)

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US10590427B2 (en) * 2014-04-17 2020-03-17 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell for expressing proteins of interest
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
SG10202110774QA (en) * 2017-03-29 2021-11-29 Boehringer Ingelheim Rcv Gmbh Recombinant host cell with altered membrane lipid composition
CN109722445A (zh) * 2017-10-27 2019-05-07 成都必高生物科技有限公司 提升溶菌酶产量及活性的方法
KR20200086303A (ko) * 2017-11-20 2020-07-16 아젠스 홀딩 비.브이. 숙주 세포에서의 향미 화합물 생산
JP7623680B2 (ja) * 2018-05-17 2025-01-29 ボルト スレッズ インコーポレイテッド 組換えタンパク質の分泌を改善するためのｓｅｃ改変株
CA3103988A1 (en) * 2018-06-27 2020-01-02 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for increased protein expression by use of transcription factors
CN112739819A (zh) * 2018-09-24 2021-04-30 中东科技大学 利用启动子工程化设计醇脱氢酶2(adh2)启动子变体
CN109439640B (zh) * 2018-10-09 2021-07-20 天津科技大学 伏马毒素降解酶FumDSB及其基因和应用
CN109439639B (zh) * 2018-10-09 2021-07-20 天津科技大学 伏马毒素降解酶FumDPS及其基因和应用
CN111378585B (zh) 2018-12-28 2023-06-16 丰益(上海)生物技术研发中心有限公司 用于表达外源基因的毕赤酵母突变株
DK3874047T4 (da) 2019-01-11 2025-02-03 Lonza Ag Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle
DK3997118T3 (da) 2019-07-11 2025-03-24 Clara Foods Co Drikkevaresammensætning, der indeholder rekombinant ovomucoid-protein
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
AU2021216950A1 (en) * 2020-02-04 2022-09-01 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof
CN111349177B (zh) * 2020-03-13 2022-04-22 西北农林科技大学 一种融合抗菌肽cat的制备方法和应用
CN111549007B (zh) * 2020-04-07 2022-10-04 天津科技大学 一种转氨酶tsta、制备方法和应用
CN111607575B (zh) * 2020-04-07 2022-10-04 天津科技大学 一种转氨酶phta、制备方法和应用
CN113088533B (zh) * 2021-04-15 2023-03-24 华中科技大学 一种高效表达藤壶粘胶蛋白的酵母工程菌及其制备方法
CN113801801B (zh) * 2021-10-08 2023-03-07 枣庄市杰诺生物酶有限公司 一株高效生产碱性果胶酶的重组菌及其应用
CN118147180A (zh) * 2024-05-09 2024-06-07 芝诺(苏州)生物科技有限公司 一种代谢工程改造毕赤酵母合成人乳铁蛋白的方法

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Also Published As

Publication number Publication date
WO2015158808A2 (en) 2015-10-22
EP4311857A2 (en) 2024-01-31
KR20170002453A (ko) 2017-01-06
CN106661541A (zh) 2017-05-10
WO2015158808A3 (en) 2016-01-28
JP7062633B2 (ja) 2022-05-06
US10865416B2 (en) 2020-12-15
IL248376A0 (en) 2016-11-30
EP3132028A2 (en) 2017-02-22
AU2015248815A1 (en) 2016-11-03
SG11201608084TA (en) 2016-10-28
SG10201809146QA (en) 2018-11-29
CA2948369C (en) 2023-09-26
IL248376B (en) 2021-04-29
EP4311857A3 (en) 2024-07-31
EP3132028B1 (en) 2023-10-25
US20170029827A1 (en) 2017-02-02
JP2020072696A (ja) 2020-05-14
BR112016023304A2 (pt) 2017-10-17
CN106661541B (zh) 2021-09-03
KR102373954B1 (ko) 2022-03-14
JP2017511148A (ja) 2017-04-20
CA2948369A1 (en) 2015-10-22

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Free format text: FORMER NAME(S): VTU TECHNOLOGY GMBH; SANDOZ AG; LONZA LTD.; BOEHRINGER INGELHEIM RCV GMBH & CO KG

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