JP2017169579A5 - - Google Patents

Info

Publication number

JP2017169579A5

JP2017169579A5 JP2017097126A JP2017097126A JP2017169579A5 JP 2017169579 A5 JP2017169579 A5 JP 2017169579A5 JP 2017097126 A JP2017097126 A JP 2017097126A JP 2017097126 A JP2017097126 A JP 2017097126A JP 2017169579 A5 JP2017169579 A5 JP 2017169579A5

Authority

JP

Japan

Prior art keywords

cell

antibody

feeder

tnfα

nucleic acid

Prior art date

2017-05-16

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 210000003719 b-lymphocyte Anatomy 0.000 claims 17
• 238000000034 method Methods 0.000 claims 12
• 210000004027 cell Anatomy 0.000 claims 9
• 239000000203 mixture Substances 0.000 claims 6
• 108090000174 Interleukin-10 Proteins 0.000 claims 5
• 108060008682 Tumor Necrosis Factor Proteins 0.000 claims 5
• 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims 5
• 108010002350 Interleukin-2 Proteins 0.000 claims 4
• 108090001005 Interleukin-6 Proteins 0.000 claims 4
• 150000007523 nucleic acids Chemical group 0.000 claims 4
• 101150095323 Adcy10 gene Proteins 0.000 claims 3
• 241000699800 Cricetinae Species 0.000 claims 3
• 238000012258 culturing Methods 0.000 claims 3
• 241000283973 Oryctolagus cuniculus Species 0.000 claims 2
• 239000000427 antigen Substances 0.000 claims 2
• 102000036639 antigens Human genes 0.000 claims 2
• 108091007433 antigens Proteins 0.000 claims 2
• 108020004707 nucleic acids Proteins 0.000 claims 2
• 102000039446 nucleic acids Human genes 0.000 claims 2
• DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims 2
• 239000000243 solution Substances 0.000 claims 2
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims 2
• MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 claims 1
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 1
• 125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 claims 1
• 239000007995 HEPES buffer Substances 0.000 claims 1
• 108090000978 Interleukin-4 Proteins 0.000 claims 1
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
• 229930182555 Penicillin Natural products 0.000 claims 1
• JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims 1
• 239000012980 RPMI-1640 medium Substances 0.000 claims 1
• 101710181056 Tumor necrosis factor ligand superfamily member 13B Proteins 0.000 claims 1
• 239000000872 buffer Substances 0.000 claims 1
• 238000005119 centrifugation Methods 0.000 claims 1
• 238000003501 co-culture Methods 0.000 claims 1
• 239000012881 co-culture medium Substances 0.000 claims 1
• 239000012228 culture supernatant Substances 0.000 claims 1
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 1
• 238000011534 incubation Methods 0.000 claims 1
• 108010074108 interleukin-21 Proteins 0.000 claims 1
• 210000003519 mature b lymphocyte Anatomy 0.000 claims 1
• 229940049954 penicillin Drugs 0.000 claims 1
• 238000003757 reverse transcription PCR Methods 0.000 claims 1
• 229940054269 sodium pyruvate Drugs 0.000 claims 1
• 229960005322 streptomycin Drugs 0.000 claims 1