CY1117850T1 - Μεθοδοι για παραγωγη χοιρειου κυκλοϊου - Google Patents
Μεθοδοι για παραγωγη χοιρειου κυκλοϊουInfo
- Publication number
- CY1117850T1 CY1117850T1 CY20161100740T CY161100740T CY1117850T1 CY 1117850 T1 CY1117850 T1 CY 1117850T1 CY 20161100740 T CY20161100740 T CY 20161100740T CY 161100740 T CY161100740 T CY 161100740T CY 1117850 T1 CY1117850 T1 CY 1117850T1
- Authority
- CY
- Cyprus
- Prior art keywords
- cells
- viral
- methods
- host cells
- supernatant
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/02—Recovery or purification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/10011—Circoviridae
- C12N2750/10051—Methods of production or purification of viral material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
Abstract
Η παρούσα εφεύρεση παρέχει μεθόδους για τον προσδιορισμό του ιικού τίτλου μίας καλλιέργειας κυττάρων ξενιστών ζώου ξενιστή μολυνθέντος με κυκλοϊό. Οι βασιζόμενες σε FACS μέθοδοι της εφεύρεσης μπορεί να περιλαμβάνουν προσδιορισμό της βιωσιμότητας των κυττάρων ξενιστών σε ένα υπερκείμενο μέσο κυτταροκαλλιέργειας και αυτών των κυττάρων που παραμένουν προσκολλημένα σε στερεό υπόστρωμα. Ανίχνευση και μέτρηση ποσοστού επί τοις εκατό κυττάρων που εκφράζουν τα ιικά αντιγόνα ORF1 και 0RF2 μπορεί να προσδιορίζουν το ιικό φορτίο των καλλιεργημένων κυττάρων-ξενιστών. Η απόδοση του ιού μπορεί να καθιερώνεται με την ανίχνευση και τη μέτρηση αμφοτέρων των αντιγόνων στα υπερκείμενα κύτταρα, για παράδειγμα (5) έως (7) ημέρες από όταν τα κύτταρα ξενιστές μεταφέρονται σε άνευ ορού μέσο. Οι μέθοδοι της εφεύρεσης μπορεί να αποδώσουν ταχεία ποσοτικά δεδομένα. Αυτό επιτρέπει την επαναλαμβανόμενη εν τη μεθόδω παρακολούθηση της ιικής παραγωγής σε όλη τη διάρκεια της περιόδου επώασης, και εύκολη επιλογή του πλέον κατάλληλου σημείου συγκομιδής.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US67089205P | 2005-04-13 | 2005-04-13 | |
PCT/US2006/014068 WO2006113435A2 (en) | 2005-04-13 | 2006-04-13 | Method for porcine circovirus production and assays for monitoring production |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1117850T1 true CY1117850T1 (el) | 2017-05-17 |
Family
ID=37115731
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20161100740T CY1117850T1 (el) | 2005-04-13 | 2016-07-27 | Μεθοδοι για παραγωγη χοιρειου κυκλοϊου |
Country Status (21)
Country | Link |
---|---|
US (2) | US7358075B2 (el) |
EP (2) | EP1869471B1 (el) |
JP (1) | JP5408998B2 (el) |
KR (1) | KR101369656B1 (el) |
CN (1) | CN101194165B (el) |
BR (1) | BRPI0607540B1 (el) |
CA (1) | CA2604691C (el) |
CR (1) | CR9511A (el) |
CY (1) | CY1117850T1 (el) |
DK (1) | DK1869471T3 (el) |
ES (1) | ES2589928T3 (el) |
HK (1) | HK1121232A1 (el) |
HR (1) | HRP20161132T1 (el) |
HU (1) | HUE028343T2 (el) |
ME (1) | ME02683B (el) |
MX (1) | MX2007012786A (el) |
PL (1) | PL1869471T3 (el) |
PT (1) | PT1869471T (el) |
RS (1) | RS55085B1 (el) |
SI (1) | SI1869471T1 (el) |
WO (1) | WO2006113435A2 (el) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2772047B1 (fr) | 1997-12-05 | 2004-04-09 | Ct Nat D Etudes Veterinaires E | Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection |
UA95602C2 (ru) | 2004-12-30 | 2011-08-25 | Берингер Ингельхейм Ветмедика, Инк. | Иммуногенная композиция цвс2 и способы приготовления такой композиции |
US7700285B1 (en) * | 2005-12-29 | 2010-04-20 | Boehringer Ingelheim Vetmedica, Inc. | PCV2 immunogenic compositions and methods of producing such compositions |
US7833707B2 (en) * | 2004-12-30 | 2010-11-16 | Boehringer Ingelheim Vetmedica, Inc. | Methods of overexpression and recovery of porcine circovirus type 2 ORF2 |
US20080226594A1 (en) * | 2005-02-03 | 2008-09-18 | Hans Nauwynck | Culturing circular ssdna viruses for the production of vaccines |
HUE051025T2 (hu) * | 2005-12-29 | 2021-01-28 | Boehringer Ingelheim Animal Health Usa Inc | Multivalens PCV2 immunogén készítmények és eljárások ilyen készítmények elõállítására |
EP3766518A1 (en) * | 2005-12-29 | 2021-01-20 | Boehringer Ingelheim Animal Health USA Inc. | Pcv2 immunogenic composition for lessening clinical symptoms in pigs |
EP2101815A4 (en) | 2006-12-11 | 2010-10-06 | Boehringer Ingelheim Vetmed | EFFICIENT PROCESS FOR THE TREATMENT OF PORCINE CIRCOVIRUS AND LAWSONIA INTRACELLULARIS INFECTIONS |
CL2007003648A1 (es) * | 2006-12-15 | 2008-04-11 | Boehringer Ingelheim Vetmed | Metodo para el tratamiento o profilaxis de infeccion por circovirus 2 porcino (pcv2) o para la reduccion de los sintomas clinicos asociados a pcv2 en animales que tienen anticuerpos anti-pcv2 y/o que tienen 1 a 22 dias de edad que comprende administr |
EP1941903A1 (en) | 2007-01-03 | 2008-07-09 | Boehringer Ingelheim Vetmedica Gmbh | Prophylaxis and treatment of PRDC |
EP1958644A1 (en) * | 2007-02-13 | 2008-08-20 | Boehringer Ingelheim Vetmedica Gmbh | Prevention and treatment of sub-clinical pcvd |
US8330873B2 (en) * | 2007-03-14 | 2012-12-11 | Larry Silver | Signal demodulator with overmodulation protection |
US8518696B2 (en) | 2007-05-11 | 2013-08-27 | Temasek Life Sciences Laboratory Limited | Production of homogeneous cell line highly permissive to porcine circovirus type 2 (PCV2) infection |
US7829274B2 (en) | 2007-09-04 | 2010-11-09 | Boehringer Ingelheim Vetmedica, Inc. | Reduction of concomitant infections in pigs by the use of PCV2 antigen |
US20110020394A1 (en) * | 2007-12-31 | 2011-01-27 | Boehringer Ingelheim Vetmedica, Inc. | Pcv2 orf2 virus like particle with foreign amino acid insertion |
BRPI0907438A2 (pt) * | 2008-01-23 | 2015-08-04 | Boehringer Ingelheim Vetmed | Composições imunogênicas para mycoplasma hyopneumoniae e pcv2 e métodos para produzir tais composições |
TWI627281B (zh) | 2009-09-02 | 2018-06-21 | 百靈佳殷格翰家畜藥品公司 | 降低pcv-2組合物殺病毒活性之方法及具有改良免疫原性之pcv-2組合物 |
CN101773667A (zh) * | 2010-01-28 | 2010-07-14 | 洛阳普莱柯生物工程有限公司 | 一种猪圆环病毒ii型疫苗制备 |
CN102690790B (zh) * | 2012-04-27 | 2013-09-25 | 成都天邦生物制品有限公司 | 猪圆环病毒2型细胞悬浮培养生产方法 |
WO2014182872A1 (en) | 2013-05-08 | 2014-11-13 | Protatek International, Inc. | Vaccine for pcv2 and mycoplasma |
KR102319843B1 (ko) | 2013-09-25 | 2021-10-29 | 조에티스 서비시즈 엘엘씨 | Pcv2b 분지형 백신 조성물 및 사용 방법 |
EP3052516B1 (en) | 2013-10-02 | 2020-01-01 | Boehringer Ingelheim Animal Health USA Inc. | Pcv2 orf2 protein variant and virus like particles composed thereof |
WO2017019404A1 (en) | 2015-07-24 | 2017-02-02 | Merial, Inc. | High throughput methods for virus quantification |
DE102018010282A1 (de) | 2018-01-17 | 2019-07-18 | Immatics US, Inc. | Verfahren zur Feststellung der Wirksamkeit von viralen Vektoren |
DE102018100967B4 (de) | 2018-01-17 | 2019-08-14 | Immatics US, Inc. | Verfahren zur feststellung der wirksamkeit von viralen vektoren |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6391314B1 (en) * | 1997-10-03 | 2002-05-21 | Merial | Porcine circoviruses vaccines diagnostic reagents |
UA78180C2 (uk) * | 1997-10-03 | 2007-03-15 | Меріаль | Кільцевий вірус свині типу ii, вакцини та діагностичні реагенти |
EP1111040A1 (en) * | 1999-12-10 | 2001-06-27 | Daniel Favre | Infection of eukaryotic cells with viruses in vitro |
CA2410229A1 (en) * | 2000-06-15 | 2001-12-20 | Purdue Research Foundation | Vaccine for congenital tremors in pigs |
DE10044648A1 (de) * | 2000-09-08 | 2002-03-21 | Aventis Behring Gmbh | Verfahren zur Vermehrung oder zur Entfernung von Cirocoviren aus biologischem Material |
DK2287288T3 (da) * | 2002-07-09 | 2013-01-07 | Baxter Int | Medium frit for animalsk protein til dyrkning af celler |
WO2005014862A1 (en) * | 2003-02-25 | 2005-02-17 | Medimmune Vaccines, Inc. | Methods of producing inflenza vaccine compositions |
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2006
- 2006-04-13 HU HUE06750177A patent/HUE028343T2/en unknown
- 2006-04-13 EP EP06750177.5A patent/EP1869471B1/en active Active
- 2006-04-13 US US11/403,497 patent/US7358075B2/en active Active
- 2006-04-13 PT PT67501775T patent/PT1869471T/pt unknown
- 2006-04-13 DK DK06750177.5T patent/DK1869471T3/en active
- 2006-04-13 ME MEP-2016-174A patent/ME02683B/me unknown
- 2006-04-13 RS RS20160699A patent/RS55085B1/sr unknown
- 2006-04-13 SI SI200632090A patent/SI1869471T1/sl unknown
- 2006-04-13 JP JP2008506739A patent/JP5408998B2/ja active Active
- 2006-04-13 WO PCT/US2006/014068 patent/WO2006113435A2/en active Application Filing
- 2006-04-13 CA CA2604691A patent/CA2604691C/en active Active
- 2006-04-13 ES ES06750177.5T patent/ES2589928T3/es active Active
- 2006-04-13 KR KR1020077026312A patent/KR101369656B1/ko active IP Right Grant
- 2006-04-13 PL PL06750177T patent/PL1869471T3/pl unknown
- 2006-04-13 BR BRPI0607540A patent/BRPI0607540B1/pt active IP Right Grant
- 2006-04-13 CN CN2006800205132A patent/CN101194165B/zh active Active
- 2006-04-13 MX MX2007012786A patent/MX2007012786A/es active IP Right Grant
- 2006-04-13 EP EP16164122.0A patent/EP3088893B1/en active Active
-
2007
- 2007-11-12 CR CR9511A patent/CR9511A/es unknown
-
2008
- 2008-04-11 US US12/101,512 patent/US7476507B2/en active Active
- 2008-11-21 HK HK08112728.6A patent/HK1121232A1/xx unknown
-
2016
- 2016-07-27 CY CY20161100740T patent/CY1117850T1/el unknown
- 2016-09-02 HR HRP20161132TT patent/HRP20161132T1/hr unknown
Also Published As
Publication number | Publication date |
---|---|
US20060246425A1 (en) | 2006-11-02 |
CR9511A (es) | 2008-01-29 |
WO2006113435A3 (en) | 2007-06-21 |
CN101194165B (zh) | 2013-01-09 |
PL1869471T3 (pl) | 2017-02-28 |
PT1869471T (pt) | 2016-08-16 |
ES2589928T3 (es) | 2016-11-17 |
BRPI0607540B1 (pt) | 2017-05-09 |
US7476507B2 (en) | 2009-01-13 |
RS55085B1 (sr) | 2016-12-30 |
MX2007012786A (es) | 2008-01-11 |
CN101194165A (zh) | 2008-06-04 |
BRPI0607540A2 (pt) | 2009-09-15 |
EP1869471A2 (en) | 2007-12-26 |
CA2604691A1 (en) | 2006-10-26 |
KR101369656B1 (ko) | 2014-03-04 |
JP5408998B2 (ja) | 2014-02-05 |
HRP20161132T1 (hr) | 2016-12-02 |
KR20070120603A (ko) | 2007-12-24 |
US20080241821A1 (en) | 2008-10-02 |
JP2008536499A (ja) | 2008-09-11 |
EP3088893A1 (en) | 2016-11-02 |
EP3088893B1 (en) | 2018-02-14 |
SI1869471T1 (sl) | 2016-09-30 |
CA2604691C (en) | 2013-12-31 |
EP1869471B1 (en) | 2016-06-08 |
HK1121232A1 (en) | 2009-04-17 |
DK1869471T3 (en) | 2016-08-01 |
HUE028343T2 (en) | 2016-12-28 |
ME02683B (me) | 2017-06-20 |
US7358075B2 (en) | 2008-04-15 |
WO2006113435A2 (en) | 2006-10-26 |
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