MX2007012786A - Ensayo para produccion de circovirus porcino. - Google Patents

Ensayo para produccion de circovirus porcino.

Info

Publication number
MX2007012786A
MX2007012786A MX2007012786A MX2007012786A MX2007012786A MX 2007012786 A MX2007012786 A MX 2007012786A MX 2007012786 A MX2007012786 A MX 2007012786A MX 2007012786 A MX2007012786 A MX 2007012786A MX 2007012786 A MX2007012786 A MX 2007012786A
Authority
MX
Mexico
Prior art keywords
cells
host cells
viral
production
methods
Prior art date
Application number
MX2007012786A
Other languages
English (en)
Inventor
Patrice Allibert
Lionel Pierre Cupillard
Jean Reyes
Original Assignee
Merial Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merial Ltd filed Critical Merial Ltd
Publication of MX2007012786A publication Critical patent/MX2007012786A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10051—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses

Abstract

La presente invencion proporciona metodos para la determinacion del titulo viral de un cultivo de celulas hospederas de animal hospedero infectadas con un circovirus. Los metodos de la invencion basados en FACS pueden incluir la determinacion de la viabilidad de las celulas hospederas en un sobrenadante de medio de cultivo de celulas y de aquellas celulas que permanecen adherentes a un soporte solido. La deteccion y la medicion del porcentaje de celulas que expresaron los antigenos virales ORF1 y ORF2 pueden determinar la carga viral de las celulas hospederas cultivadas. El rendimiento del virus se puede establecer por la deteccion y medicion de ambos antigenos en las celulas sobrenadantes, por ejemplo (5) a (7) dias desde cuando las celulas hospederas se transfieren a un medio libre de suero. Los metodos de la invencion pueden producir datos cuantitativos rapidos. Esto permite el monitoreo repetido en el proceso de la produccion viral por todo el periodo de incubacion, y la seleccion adecuada del punto de recoleccion mucho mas apropiado.
MX2007012786A 2005-04-13 2006-04-13 Ensayo para produccion de circovirus porcino. MX2007012786A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67089205P 2005-04-13 2005-04-13
PCT/US2006/014068 WO2006113435A2 (en) 2005-04-13 2006-04-13 Method for porcine circovirus production and assays for monitoring production

Publications (1)

Publication Number Publication Date
MX2007012786A true MX2007012786A (es) 2008-01-11

Family

ID=37115731

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2007012786A MX2007012786A (es) 2005-04-13 2006-04-13 Ensayo para produccion de circovirus porcino.

Country Status (21)

Country Link
US (2) US7358075B2 (es)
EP (2) EP1869471B1 (es)
JP (1) JP5408998B2 (es)
KR (1) KR101369656B1 (es)
CN (1) CN101194165B (es)
BR (1) BRPI0607540B1 (es)
CA (1) CA2604691C (es)
CR (1) CR9511A (es)
CY (1) CY1117850T1 (es)
DK (1) DK1869471T3 (es)
ES (1) ES2589928T3 (es)
HK (1) HK1121232A1 (es)
HR (1) HRP20161132T1 (es)
HU (1) HUE028343T2 (es)
ME (1) ME02683B (es)
MX (1) MX2007012786A (es)
PL (1) PL1869471T3 (es)
PT (1) PT1869471T (es)
RS (1) RS55085B1 (es)
SI (1) SI1869471T1 (es)
WO (1) WO2006113435A2 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2772047B1 (fr) 1997-12-05 2004-04-09 Ct Nat D Etudes Veterinaires E Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection
US7700285B1 (en) * 2005-12-29 2010-04-20 Boehringer Ingelheim Vetmedica, Inc. PCV2 immunogenic compositions and methods of producing such compositions
US7833707B2 (en) * 2004-12-30 2010-11-16 Boehringer Ingelheim Vetmedica, Inc. Methods of overexpression and recovery of porcine circovirus type 2 ORF2
UA95602C2 (ru) 2004-12-30 2011-08-25 Берингер Ингельхейм Ветмедика, Инк. Иммуногенная композиция цвс2 и способы приготовления такой композиции
US20080226594A1 (en) * 2005-02-03 2008-09-18 Hans Nauwynck Culturing circular ssdna viruses for the production of vaccines
DK2371382T3 (en) 2005-12-29 2016-06-13 Boehringer Ingelheim Vetmedica Inc Use of a PCV2 immunogenic composition to reduce the clinical signs in swine
TWI528970B (zh) 2005-12-29 2016-04-11 百靈佳殷格翰家畜藥品公司 多價第二型豬環狀病毒（ｐｃｖ２）免疫組合物及製備該組合物之方法
EP2859900A1 (en) 2006-12-11 2015-04-15 Boehringer Ingelheim Vetmedica, Inc. Effective method of treatment of porcine circovirus and lawsonia intracellularis infections
CN107412761A (zh) 2006-12-15 2017-12-01 贝林格尔.英格海姆维特梅迪卡有限公司 以pcv2抗原治疗猪
EP1941903A1 (en) 2007-01-03 2008-07-09 Boehringer Ingelheim Vetmedica Gmbh Prophylaxis and treatment of PRDC
EP1958644A1 (en) * 2007-02-13 2008-08-20 Boehringer Ingelheim Vetmedica Gmbh Prevention and treatment of sub-clinical pcvd
US8502920B2 (en) * 2007-03-14 2013-08-06 Vyacheslav Shyshkin Method and apparatus for extracting a desired television signal from a wideband IF input
US8518696B2 (en) 2007-05-11 2013-08-27 Temasek Life Sciences Laboratory Limited Production of homogeneous cell line highly permissive to porcine circovirus type 2 (PCV2) infection
US7829274B2 (en) * 2007-09-04 2010-11-09 Boehringer Ingelheim Vetmedica, Inc. Reduction of concomitant infections in pigs by the use of PCV2 antigen
WO2009088950A2 (en) * 2007-12-31 2009-07-16 Boehringer Ingelheim Vetmedica, Inc. Pcv2 orf2 virus like particle with foreign amino acid insertion
CA2712006A1 (en) 2008-01-23 2009-10-15 Boehringer Ingelheim Vetmedica, Inc. Pcv2 mycoplasma hyopneumoniae immunogenic compositions and methods of producing such compositions
AR078253A1 (es) 2009-09-02 2011-10-26 Boehringer Ingelheim Vetmed Metodos para reducir la actividad antivirica en composiciones pcv-2 y composiciones pcv-2 con mejor inmunogenicidad
CN101773667A (zh) * 2010-01-28 2010-07-14 洛阳普莱柯生物工程有限公司 一种猪圆环病毒ii型疫苗制备
CN102690790B (zh) * 2012-04-27 2013-09-25 成都天邦生物制品有限公司 猪圆环病毒2型细胞悬浮培养生产方法
WO2014182872A1 (en) 2013-05-08 2014-11-13 Protatek International, Inc. Vaccine for pcv2 and mycoplasma
RU2662685C2 (ru) 2013-09-25 2018-07-26 Зоэтис Сервисиз Ллс Pcv2b дивергентная вакцинная композиция и способы её применения
DK3052516T3 (da) 2013-10-02 2020-03-23 Boehringer Ingelheim Animal Health Usa Inc PCV2-ORF2-proteinvariant og viruslignende partikler sammensat deraf
US10663468B2 (en) 2015-07-24 2020-05-26 Boehringer Ingelheim Animal Health USA Inc. High throughput methods for virus quantification
DE102018010282A1 (de) 2018-01-17 2019-07-18 Immatics US, Inc. Verfahren zur Feststellung der Wirksamkeit von viralen Vektoren
DE102018100967B4 (de) 2018-01-17 2019-08-14 Immatics US, Inc. Verfahren zur feststellung der wirksamkeit von viralen vektoren

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6391314B1 (en) * 1997-10-03 2002-05-21 Merial Porcine circoviruses vaccines diagnostic reagents
UA78180C2 (uk) * 1997-10-03 2007-03-15 Меріаль Кільцевий вірус свині типу ii, вакцини та діагностичні реагенти
EP1111040A1 (en) * 1999-12-10 2001-06-27 Daniel Favre Infection of eukaryotic cells with viruses in vitro
EP1290016A2 (en) * 2000-06-15 2003-03-12 Purdue Research Foundation Vaccine for congenital tremors in pigs
DE10044648A1 (de) * 2000-09-08 2002-03-21 Aventis Behring Gmbh Verfahren zur Vermehrung oder zur Entfernung von Cirocoviren aus biologischem Material
EP2287288B1 (en) * 2002-07-09 2012-11-07 Baxter International Inc. Animal protein free media for cultivation of cells
CN100582244C (zh) * 2003-02-25 2010-01-20 米迪缪尼有限公司 制备流感疫苗组合物的方法

Also Published As

Publication number Publication date
HK1121232A1 (en) 2009-04-17
WO2006113435A3 (en) 2007-06-21
RS55085B1 (sr) 2016-12-30
CN101194165A (zh) 2008-06-04
HRP20161132T1 (hr) 2016-12-02
CA2604691C (en) 2013-12-31
PT1869471T (pt) 2016-08-16
BRPI0607540A2 (pt) 2009-09-15
JP2008536499A (ja) 2008-09-11
PL1869471T3 (pl) 2017-02-28
HUE028343T2 (en) 2016-12-28
CY1117850T1 (el) 2017-05-17
CR9511A (es) 2008-01-29
KR20070120603A (ko) 2007-12-24
DK1869471T3 (en) 2016-08-01
US7358075B2 (en) 2008-04-15
EP1869471B1 (en) 2016-06-08
WO2006113435A2 (en) 2006-10-26
ES2589928T3 (es) 2016-11-17
BRPI0607540B1 (pt) 2017-05-09
CA2604691A1 (en) 2006-10-26
CN101194165B (zh) 2013-01-09
SI1869471T1 (sl) 2016-09-30
JP5408998B2 (ja) 2014-02-05
EP3088893B1 (en) 2018-02-14
EP1869471A2 (en) 2007-12-26
ME02683B (me) 2017-06-20
US7476507B2 (en) 2009-01-13
EP3088893A1 (en) 2016-11-02
US20080241821A1 (en) 2008-10-02
KR101369656B1 (ko) 2014-03-04
US20060246425A1 (en) 2006-11-02

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