HRP20161132T1 - Postupak proizvodnje svinjskog cirkovirusa - Google Patents

Postupak proizvodnje svinjskog cirkovirusa Download PDF

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Publication number
HRP20161132T1
HRP20161132T1 HRP20161132TT HRP20161132T HRP20161132T1 HR P20161132 T1 HRP20161132 T1 HR P20161132T1 HR P20161132T T HRP20161132T T HR P20161132TT HR P20161132 T HRP20161132 T HR P20161132T HR P20161132 T1 HRP20161132 T1 HR P20161132T1
Authority
HR
Croatia
Prior art keywords
circovirus
orf2
host cell
orf1
expression
Prior art date
Application number
HRP20161132TT
Other languages
English (en)
Inventor
Patrice Allibert
Lionel Pierre Cupillard
Jean Reyes
Original Assignee
Merial, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merial, Inc. filed Critical Merial, Inc.
Publication of HRP20161132T1 publication Critical patent/HRP20161132T1/hr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10051—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses

Claims (5)

1. Postupak proizvodnje cirkovirusa, naznačen time, da obuhvaća sljedeće korake: kalemljenje kulture stanice domaćina s kulturom cirkovirusa; inkubacija kulture stanice domaćina bez seruma od fetusa mladunca; nadziranje (i) životne aktivnosti stanica domaćina, i (ii) ekspresije ORF1 i ORF2 putem stanica domaćina; i sabiranje cirkovirusa iz kulture stanice domaćina kada je ekspresija ORF2 otprilike ista u neprianjajućim stanicama kao i u prianjajućim stanicama od kulture stanice domaćina; te time, da cirkovirus je PCV2.
2. Postupak prema zahtjevu 1, naznačen time, da je prinos cirkovirusa u kulturi sjemena određen pomoću nadziranja ekspresije cirkovirusnih antigena ORF1 i ORF2 u stanicama domaćina koje su isplivale na površinu, uporabom postupka koji se temelji na sortiranju fluorescentnih stanica protutijela (FACS-fluorescent antibody cell sorting).
3. Postupak prema zahtjevu 1 ili 2, naznačen time, da se ekspresija cirkovirusnih antigena ORF1 i ORF2 u stanici domaćina koja je inficirana virusom, nadzire uporabom postupka koji se temelji na (FACS) sortiranju fluorescentnih stanica protutijela, te time, da se proizvodnja cirkovirusnog antigena odnosi na postotak pozitivnih stanica otvorenog okvira čitanja 1 (ORF1-open reading frame 1) i otvorenog okvira čitanja 2 (ORF2-open reading frame 2).
4. Postupak prema bilo kojem od zahtjeva 1 do 3, naznačen time, da se životna održivost stanica određuje pomoću mjerenja primanja propidijevog jodida uporabom protočne citometrije.
5. Postupak prema bilo kojem od zahtjeva 1 do 4, naznačen time, da stanica domaćina je PK-15.
HRP20161132TT 2005-04-13 2016-09-02 Postupak proizvodnje svinjskog cirkovirusa HRP20161132T1 (hr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US67089205P 2005-04-13 2005-04-13
PCT/US2006/014068 WO2006113435A2 (en) 2005-04-13 2006-04-13 Method for porcine circovirus production and assays for monitoring production
EP06750177.5A EP1869471B1 (en) 2005-04-13 2006-04-13 Method for porcine circovirus production

Publications (1)

Publication Number Publication Date
HRP20161132T1 true HRP20161132T1 (hr) 2016-12-02

Family

ID=37115731

Family Applications (1)

Application Number Title Priority Date Filing Date
HRP20161132TT HRP20161132T1 (hr) 2005-04-13 2016-09-02 Postupak proizvodnje svinjskog cirkovirusa

Country Status (21)

Country Link
US (2) US7358075B2 (hr)
EP (2) EP1869471B1 (hr)
JP (1) JP5408998B2 (hr)
KR (1) KR101369656B1 (hr)
CN (1) CN101194165B (hr)
BR (1) BRPI0607540B1 (hr)
CA (1) CA2604691C (hr)
CR (1) CR9511A (hr)
CY (1) CY1117850T1 (hr)
DK (1) DK1869471T3 (hr)
ES (1) ES2589928T3 (hr)
HK (1) HK1121232A1 (hr)
HR (1) HRP20161132T1 (hr)
HU (1) HUE028343T2 (hr)
ME (1) ME02683B (hr)
MX (1) MX2007012786A (hr)
PL (1) PL1869471T3 (hr)
PT (1) PT1869471T (hr)
RS (1) RS55085B1 (hr)
SI (1) SI1869471T1 (hr)
WO (1) WO2006113435A2 (hr)

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UA95602C2 (ru) 2004-12-30 2011-08-25 Берингер Ингельхейм Ветмедика, Инк. Иммуногенная композиция цвс2 и способы приготовления такой композиции
US7700285B1 (en) * 2005-12-29 2010-04-20 Boehringer Ingelheim Vetmedica, Inc. PCV2 immunogenic compositions and methods of producing such compositions
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KR102319843B1 (ko) 2013-09-25 2021-10-29 조에티스 서비시즈 엘엘씨 Pcv2b 분지형 백신 조성물 및 사용 방법
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Also Published As

Publication number Publication date
US20060246425A1 (en) 2006-11-02
CR9511A (es) 2008-01-29
WO2006113435A3 (en) 2007-06-21
CN101194165B (zh) 2013-01-09
PL1869471T3 (pl) 2017-02-28
PT1869471T (pt) 2016-08-16
ES2589928T3 (es) 2016-11-17
BRPI0607540B1 (pt) 2017-05-09
US7476507B2 (en) 2009-01-13
RS55085B1 (sr) 2016-12-30
MX2007012786A (es) 2008-01-11
CN101194165A (zh) 2008-06-04
BRPI0607540A2 (pt) 2009-09-15
EP1869471A2 (en) 2007-12-26
CA2604691A1 (en) 2006-10-26
KR101369656B1 (ko) 2014-03-04
JP5408998B2 (ja) 2014-02-05
KR20070120603A (ko) 2007-12-24
US20080241821A1 (en) 2008-10-02
JP2008536499A (ja) 2008-09-11
EP3088893A1 (en) 2016-11-02
CY1117850T1 (el) 2017-05-17
EP3088893B1 (en) 2018-02-14
SI1869471T1 (sl) 2016-09-30
CA2604691C (en) 2013-12-31
EP1869471B1 (en) 2016-06-08
HK1121232A1 (en) 2009-04-17
DK1869471T3 (en) 2016-08-01
HUE028343T2 (en) 2016-12-28
ME02683B (me) 2017-06-20
US7358075B2 (en) 2008-04-15
WO2006113435A2 (en) 2006-10-26

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