JP2016513478A5 - - Google Patents
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- Publication number
- JP2016513478A5 JP2016513478A5 JP2016503216A JP2016503216A JP2016513478A5 JP 2016513478 A5 JP2016513478 A5 JP 2016513478A5 JP 2016503216 A JP2016503216 A JP 2016503216A JP 2016503216 A JP2016503216 A JP 2016503216A JP 2016513478 A5 JP2016513478 A5 JP 2016513478A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- culture medium
- insulin
- cystine
- copper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 64
- 238000000034 method Methods 0.000 claims 57
- 239000006143 cell culture medium Substances 0.000 claims 49
- 102000004877 Insulin Human genes 0.000 claims 32
- 108090001061 Insulin Proteins 0.000 claims 32
- 229940125396 insulin Drugs 0.000 claims 32
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 claims 31
- 229960003067 cystine Drugs 0.000 claims 31
- 229960000397 bevacizumab Drugs 0.000 claims 21
- 239000012634 fragment Substances 0.000 claims 21
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 20
- 229940108928 copper Drugs 0.000 claims 20
- 229910052802 copper Inorganic materials 0.000 claims 20
- 239000010949 copper Substances 0.000 claims 20
- 210000004962 mammalian cell Anatomy 0.000 claims 16
- 238000004113 cell culture Methods 0.000 claims 14
- 210000004027 cell Anatomy 0.000 claims 10
- 238000012258 culturing Methods 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 239000002609 medium Substances 0.000 claims 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 3
- 235000018417 cysteine Nutrition 0.000 claims 3
- 241001465754 Metazoa Species 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 230000010261 cell growth Effects 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 230000001186 cumulative effect Effects 0.000 claims 1
- 230000002708 enhancing effect Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361801247P | 2013-03-15 | 2013-03-15 | |
| US61/801,247 | 2013-03-15 | ||
| PCT/US2014/029758 WO2014145091A1 (en) | 2013-03-15 | 2014-03-14 | Cell culture media and methods of antibody production |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019112773A Division JP2019193643A (ja) | 2013-03-15 | 2019-06-18 | 細胞培養培地及び抗体を産生する方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016513478A JP2016513478A (ja) | 2016-05-16 |
| JP2016513478A5 true JP2016513478A5 (enExample) | 2017-04-13 |
Family
ID=51537877
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016503216A Pending JP2016513478A (ja) | 2013-03-15 | 2014-03-14 | 細胞培養培地及び抗体を産生する方法 |
| JP2019112773A Pending JP2019193643A (ja) | 2013-03-15 | 2019-06-18 | 細胞培養培地及び抗体を産生する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019112773A Pending JP2019193643A (ja) | 2013-03-15 | 2019-06-18 | 細胞培養培地及び抗体を産生する方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US9441035B2 (enExample) |
| EP (1) | EP2968544A4 (enExample) |
| JP (2) | JP2016513478A (enExample) |
| KR (1) | KR102202476B1 (enExample) |
| CN (1) | CN105246510A (enExample) |
| AR (1) | AR095348A1 (enExample) |
| BR (1) | BR112015022529A2 (enExample) |
| CA (1) | CA2903589A1 (enExample) |
| HK (1) | HK1213179A1 (enExample) |
| MX (1) | MX366112B (enExample) |
| RU (1) | RU2015144020A (enExample) |
| WO (1) | WO2014145091A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HRP20200768T4 (hr) | 2009-08-11 | 2025-03-28 | F. Hoffmann - La Roche Ag | Proizvodnja proteina u mediju za uzgoj stanica bez glutamina |
| KR102007930B1 (ko) | 2014-12-31 | 2019-08-06 | 주식회사 엘지화학 | 재조합 당단백질의 글리코실화 조절 방법 |
| AR104050A1 (es) * | 2015-03-26 | 2017-06-21 | Chugai Pharmaceutical Co Ltd | Proceso de producción con iones de cobre controlados |
| CA3041533A1 (en) * | 2016-11-07 | 2018-05-11 | Seattle Genetics, Inc. | Distribution of engineered-cysteine caps |
| GB201708655D0 (en) * | 2017-05-31 | 2017-07-12 | Ucb Biopharma Sprl | Cell culture methods |
| MX2020006639A (es) | 2017-12-22 | 2020-09-14 | Regeneron Pharma | Sistema y metodo para caracterizar las impurezas de un producto farmaceutico. |
| CN108823267B (zh) * | 2018-06-25 | 2020-05-08 | 深圳市菲鹏生物制药股份有限公司 | 调节cho-k1表达系统所分泌抗体的酸性峰含量的方法 |
| US20240043486A1 (en) * | 2020-12-08 | 2024-02-08 | Partner Therapeutics, Inc. | Manufacture of granulocyte macrophage-colony stimulating factor |
| JP2023554489A (ja) * | 2020-12-22 | 2023-12-27 | ノバルティス アーゲー | 細胞培養中の分泌された組換え発現タンパク質中のシステイン残基の酸化レベルを低減するための方法 |
| EP4334356A1 (en) * | 2021-05-03 | 2024-03-13 | Boehringer Ingelheim International Gmbh | Method for producing spesolimab |
| WO2023194946A1 (en) * | 2022-04-06 | 2023-10-12 | Kashiv Biosciences, Llc | An improved cell culture process for production of protein |
| KR20250100741A (ko) * | 2022-11-09 | 2025-07-03 | 인포스 아게 | 응축을 방지하는 인큐베이터 |
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-
2014
- 2014-03-14 JP JP2016503216A patent/JP2016513478A/ja active Pending
- 2014-03-14 CN CN201480027392.9A patent/CN105246510A/zh active Pending
- 2014-03-14 HK HK16101113.2A patent/HK1213179A1/zh unknown
- 2014-03-14 EP EP14763258.2A patent/EP2968544A4/en not_active Withdrawn
- 2014-03-14 MX MX2015011781A patent/MX366112B/es active IP Right Grant
- 2014-03-14 KR KR1020157029225A patent/KR102202476B1/ko active Active
- 2014-03-14 CA CA2903589A patent/CA2903589A1/en not_active Abandoned
- 2014-03-14 US US14/211,467 patent/US9441035B2/en active Active
- 2014-03-14 BR BR112015022529A patent/BR112015022529A2/pt not_active Application Discontinuation
- 2014-03-14 AR ARP140101052A patent/AR095348A1/es unknown
- 2014-03-14 RU RU2015144020A patent/RU2015144020A/ru not_active Application Discontinuation
- 2014-03-14 WO PCT/US2014/029758 patent/WO2014145091A1/en not_active Ceased
-
2015
- 2015-09-11 US US14/852,382 patent/US20160102141A1/en not_active Abandoned
-
2016
- 2016-07-29 US US15/224,113 patent/US20170051049A1/en not_active Abandoned
-
2019
- 2019-06-18 JP JP2019112773A patent/JP2019193643A/ja active Pending
- 2019-10-18 US US16/657,844 patent/US20200291103A1/en not_active Abandoned
- 2019-11-15 US US16/685,798 patent/US20200347122A1/en not_active Abandoned
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