JP2016513478A5 - - Google Patents
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- Publication number
- JP2016513478A5 JP2016513478A5 JP2016503216A JP2016503216A JP2016513478A5 JP 2016513478 A5 JP2016513478 A5 JP 2016513478A5 JP 2016503216 A JP2016503216 A JP 2016503216A JP 2016503216 A JP2016503216 A JP 2016503216A JP 2016513478 A5 JP2016513478 A5 JP 2016513478A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- culture medium
- insulin
- cystine
- copper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 64
- 238000000034 method Methods 0.000 claims 57
- 239000006143 cell culture medium Substances 0.000 claims 49
- 102000004877 Insulin Human genes 0.000 claims 32
- 108090001061 Insulin Proteins 0.000 claims 32
- 229940125396 insulin Drugs 0.000 claims 32
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 claims 31
- 229960003067 cystine Drugs 0.000 claims 31
- 229960000397 bevacizumab Drugs 0.000 claims 21
- 239000012634 fragment Substances 0.000 claims 21
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 20
- 229940108928 copper Drugs 0.000 claims 20
- 229910052802 copper Inorganic materials 0.000 claims 20
- 239000010949 copper Substances 0.000 claims 20
- 210000004962 mammalian cell Anatomy 0.000 claims 16
- 238000004113 cell culture Methods 0.000 claims 14
- 210000004027 cell Anatomy 0.000 claims 10
- 238000012258 culturing Methods 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 239000002609 medium Substances 0.000 claims 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 3
- 235000018417 cysteine Nutrition 0.000 claims 3
- 241001465754 Metazoa Species 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 230000010261 cell growth Effects 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 230000001186 cumulative effect Effects 0.000 claims 1
- 230000002708 enhancing effect Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361801247P | 2013-03-15 | 2013-03-15 | |
| US61/801,247 | 2013-03-15 | ||
| PCT/US2014/029758 WO2014145091A1 (en) | 2013-03-15 | 2014-03-14 | Cell culture media and methods of antibody production |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019112773A Division JP2019193643A (ja) | 2013-03-15 | 2019-06-18 | 細胞培養培地及び抗体を産生する方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016513478A JP2016513478A (ja) | 2016-05-16 |
| JP2016513478A5 true JP2016513478A5 (enExample) | 2017-04-13 |
Family
ID=51537877
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016503216A Pending JP2016513478A (ja) | 2013-03-15 | 2014-03-14 | 細胞培養培地及び抗体を産生する方法 |
| JP2019112773A Pending JP2019193643A (ja) | 2013-03-15 | 2019-06-18 | 細胞培養培地及び抗体を産生する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019112773A Pending JP2019193643A (ja) | 2013-03-15 | 2019-06-18 | 細胞培養培地及び抗体を産生する方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US9441035B2 (enExample) |
| EP (1) | EP2968544A4 (enExample) |
| JP (2) | JP2016513478A (enExample) |
| KR (1) | KR102202476B1 (enExample) |
| CN (1) | CN105246510A (enExample) |
| AR (1) | AR095348A1 (enExample) |
| BR (1) | BR112015022529A2 (enExample) |
| CA (1) | CA2903589A1 (enExample) |
| HK (1) | HK1213179A1 (enExample) |
| MX (1) | MX366112B (enExample) |
| RU (1) | RU2015144020A (enExample) |
| WO (1) | WO2014145091A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2012001742A (es) | 2009-08-11 | 2012-03-21 | Genentech Inc | Produccion de proteinas en medios de cultivo de celulas libres de glutamina. |
| KR102007930B1 (ko) | 2014-12-31 | 2019-08-06 | 주식회사 엘지화학 | 재조합 당단백질의 글리코실화 조절 방법 |
| AR104050A1 (es) | 2015-03-26 | 2017-06-21 | Chugai Pharmaceutical Co Ltd | Proceso de producción con iones de cobre controlados |
| KR102595048B1 (ko) * | 2016-11-07 | 2023-10-26 | 씨젠 인크. | 조작된-시스테인 캡의 분포 |
| GB201708655D0 (en) | 2017-05-31 | 2017-07-12 | Ucb Biopharma Sprl | Cell culture methods |
| EP3727629A1 (en) | 2017-12-22 | 2020-10-28 | Regeneron Pharmaceuticals, Inc. | System and method for characterizing drug product impurities |
| CN108823267B (zh) * | 2018-06-25 | 2020-05-08 | 深圳市菲鹏生物制药股份有限公司 | 调节cho-k1表达系统所分泌抗体的酸性峰含量的方法 |
| US20240043486A1 (en) * | 2020-12-08 | 2024-02-08 | Partner Therapeutics, Inc. | Manufacture of granulocyte macrophage-colony stimulating factor |
| CN116635416A (zh) * | 2020-12-22 | 2023-08-22 | 诺华股份有限公司 | 用于在细胞培养期间降低分泌的重组表达蛋白质中半胱氨酸残基的氧化水平的方法 |
| WO2022233770A1 (en) * | 2021-05-03 | 2022-11-10 | Boehringer Ingelheim International Gmbh | Method for producing spesolimab |
| AU2023250258A1 (en) * | 2022-04-06 | 2024-11-14 | Kashiv Biosciences, Llc | An improved cell culture process for production of protein |
| EP4587552A1 (en) * | 2022-11-09 | 2025-07-23 | Infors AG | Incubator avoiding condensation |
Family Cites Families (84)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4419446A (en) | 1980-12-31 | 1983-12-06 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant DNA process utilizing a papilloma virus DNA as a vector |
| NZ201705A (en) | 1981-08-31 | 1986-03-14 | Genentech Inc | Recombinant dna method for production of hepatitis b surface antigen in yeast |
| US4601978A (en) | 1982-11-24 | 1986-07-22 | The Regents Of The University Of California | Mammalian metallothionein promoter system |
| US4560655A (en) | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
| US4657866A (en) | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| US4879231A (en) | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
| GB8516415D0 (en) | 1985-06-28 | 1985-07-31 | Celltech Ltd | Culture of animal cells |
| US4676980A (en) | 1985-09-23 | 1987-06-30 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target specific cross-linked heteroantibodies |
| US6548640B1 (en) | 1986-03-27 | 2003-04-15 | Btg International Limited | Altered antibodies |
| US4927762A (en) | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
| GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| AU632065B2 (en) | 1988-09-23 | 1992-12-17 | Novartis Vaccines And Diagnostics, Inc. | Cell culture medium for enhanced cell growth, culture longevity and product expression |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| ATE102631T1 (de) | 1988-11-11 | 1994-03-15 | Medical Res Council | Klonierung von immunglobulin sequenzen aus den variabelen domaenen. |
| FR2646437B1 (fr) | 1989-04-28 | 1991-08-30 | Transgene Sa | Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant |
| EP0402226A1 (en) | 1989-06-06 | 1990-12-12 | Institut National De La Recherche Agronomique | Transformation vectors for yeast yarrowia |
| DE3920358A1 (de) | 1989-06-22 | 1991-01-17 | Behringwerke Ag | Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung |
| DE69029036T2 (de) | 1989-06-29 | 1997-05-22 | Medarex Inc | Bispezifische reagenzien für die aids-therapie |
| US5959177A (en) | 1989-10-27 | 1999-09-28 | The Scripps Research Institute | Transgenic plants expressing assembled secretory antibodies |
| US6075181A (en) | 1990-01-12 | 2000-06-13 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| CA2050918A1 (en) | 1990-01-12 | 1991-07-13 | Raju Kucherlapati | Generation of xenogeneic antibodies |
| US6150584A (en) | 1990-01-12 | 2000-11-21 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| WO1996033735A1 (en) | 1995-04-27 | 1996-10-31 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| DE69133476T2 (de) | 1990-08-29 | 2006-01-05 | GenPharm International, Inc., Palo Alto | Transgene Mäuse fähig zur Produktion heterologer Antikörper |
| US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| US5122469A (en) * | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| EP0564531B1 (en) | 1990-12-03 | 1998-03-25 | Genentech, Inc. | Enrichment method for variant proteins with altered binding properties |
| US5571894A (en) | 1991-02-05 | 1996-11-05 | Ciba-Geigy Corporation | Recombinant antibodies specific for a growth factor receptor |
| US6582959B2 (en) | 1991-03-29 | 2003-06-24 | Genentech, Inc. | Antibodies to vascular endothelial cell growth factor |
| GB9114948D0 (en) | 1991-07-11 | 1991-08-28 | Pfizer Ltd | Process for preparing sertraline intermediates |
| US7018809B1 (en) | 1991-09-19 | 2006-03-28 | Genentech, Inc. | Expression of functional antibody fragments |
| EP0605522B1 (en) | 1991-09-23 | 1999-06-23 | Medical Research Council | Methods for the production of humanized antibodies |
| FI941572L (fi) | 1991-10-07 | 1994-05-27 | Oncologix Inc | Anti-erbB-2-monoklonaalisten vasta-aineiden yhdistelmä ja käyttömenetelmä |
| WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
| US5667988A (en) | 1992-01-27 | 1997-09-16 | The Scripps Research Institute | Methods for producing antibody libraries using universal or randomized immunoglobulin light chains |
| ATE419355T1 (de) | 1992-02-06 | 2009-01-15 | Novartis Vaccines & Diagnostic | Marker für krebs und biosynthetisches bindeprotein dafür |
| JPH08500017A (ja) | 1992-08-17 | 1996-01-09 | ジェネンテク，インコーポレイテッド | 二特異的免疫アドヘジン |
| WO1994011026A2 (en) | 1992-11-13 | 1994-05-26 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human b lymphocyte restricted differentiation antigen for treatment of b cell lymphoma |
| US5789199A (en) | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5840523A (en) | 1995-03-01 | 1998-11-24 | Genetech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
| US5731168A (en) | 1995-03-01 | 1998-03-24 | Genentech, Inc. | Method for making heteromultimeric polypeptides |
| US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| US5869046A (en) | 1995-04-14 | 1999-02-09 | Genentech, Inc. | Altered polypeptides with increased half-life |
| CA2219486A1 (en) | 1995-04-28 | 1996-10-31 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| EP0921855B1 (en) | 1996-08-30 | 2003-11-19 | Upfront Chromatography A/S | Isolation of immunoglobulins |
| DK1500329T3 (da) | 1996-12-03 | 2012-07-09 | Amgen Fremont Inc | Humane antistoffer, der specifikt binder TNF-alfa |
| US20080318254A9 (en) | 1997-03-10 | 2008-12-25 | The Regents Of The University Of California | PSCA antibodies and hybridomas producing them |
| US20020032315A1 (en) | 1997-08-06 | 2002-03-14 | Manuel Baca | Anti-vegf antibodies |
| US6884879B1 (en) * | 1997-04-07 | 2005-04-26 | Genentech, Inc. | Anti-VEGF antibodies |
| ATE293640T1 (de) | 1997-04-07 | 2005-05-15 | Genentech Inc | Anti-vegf antikörper |
| US20020173629A1 (en) | 1997-05-05 | 2002-11-21 | Aya Jakobovits | Human monoclonal antibodies to epidermal growth factor receptor |
| US6040498A (en) | 1998-08-11 | 2000-03-21 | North Caroline State University | Genetically engineered duckweed |
| US6610833B1 (en) | 1997-11-24 | 2003-08-26 | The Institute For Human Genetics And Biochemistry | Monoclonal human natural antibodies |
| WO1999029888A1 (en) | 1997-12-05 | 1999-06-17 | The Scripps Research Institute | Humanization of murine antibody |
| US6528286B1 (en) * | 1998-05-29 | 2003-03-04 | Genentech, Inc. | Mammalian cell culture process for producing glycoproteins |
| DE60039596D1 (de) | 1999-03-30 | 2008-09-04 | Japan Tobacco Inc | Verfahren zur herstellung von monoklonalen antikörpern |
| DE60031999T2 (de) | 1999-10-04 | 2007-06-06 | Novartis Vaccines and Diagnostics, Inc., Emeryville | Stabilisierte flüssige polypeptid-haltige pharmazeutische zusammensetzungen |
| ES2248127T3 (es) | 1999-10-04 | 2006-03-16 | Medicago Inc. | Metodo para regular la transcripcion de genes foraneos en presencia de nigtrogeno. |
| US7125978B1 (en) | 1999-10-04 | 2006-10-24 | Medicago Inc. | Promoter for regulating expression of foreign genes |
| NZ526173A (en) * | 2000-11-06 | 2005-03-24 | Invitrogen Corp | Dry powder cells and cell culture reagents and methods of production thereof |
| CA2447114A1 (en) | 2001-05-16 | 2002-11-21 | Abgenix, Inc. | Human antipneumococcal antibodies from non-human animals |
| WO2003046162A2 (en) * | 2001-11-28 | 2003-06-05 | Polymun Scientific Immunbiologische Forschung Gmbh | Process for the production of polypeptides in mammalian cell cultures |
| CN1703233A (zh) | 2002-07-12 | 2005-11-30 | 米德列斯公司 | 防止蛋白氧化降解的方法和组合物 |
| CN1761482A (zh) | 2003-01-17 | 2006-04-19 | 纽约州立大学研究基金会 | 与胰腺癌相关的抗原、针对它的抗体以及诊断和治疗方法 |
| US7456264B2 (en) | 2003-05-21 | 2008-11-25 | Medarex, Inc. | Human monoclonal antibodies against Bacillus anthracis protective antigen |
| US20060115901A1 (en) * | 2004-10-22 | 2006-06-01 | Bahram Valamehr | Method and media for single cell serum-free culture of CHO cells |
| US8530192B2 (en) * | 2005-02-11 | 2013-09-10 | Novo Nordisk Healthcare Ag | Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate |
| CN100362098C (zh) * | 2005-09-29 | 2008-01-16 | 华东理工大学 | 适用于多种动物细胞大规模培养的无血清培养基 |
| JP4976502B2 (ja) * | 2007-10-15 | 2012-07-18 | 中外製薬株式会社 | 抗体の製造方法 |
| EP2334323A2 (en) | 2008-09-10 | 2011-06-22 | F. Hoffmann-La Roche AG | Compositions and methods for the prevention of oxidative degradation of proteins |
| KR20110074564A (ko) * | 2008-09-24 | 2011-06-30 | 메디뮨 엘엘씨 | 세포 배양, 바이러스 증식 및 바이러스 정제를 위한 방법 |
| JP5749930B2 (ja) * | 2008-10-28 | 2015-07-15 | 中外製薬株式会社 | ペプチド含有動物細胞培養用培地 |
| CN101603026B (zh) * | 2009-06-19 | 2011-01-19 | 华东理工大学 | 适于动物细胞产品生产的无动物来源低蛋白培养基 |
| CN102471377B (zh) * | 2009-07-24 | 2015-04-22 | 霍夫曼-拉罗奇有限公司 | 优化抗体的生产 |
| MX2012001742A (es) * | 2009-08-11 | 2012-03-21 | Genentech Inc | Produccion de proteinas en medios de cultivo de celulas libres de glutamina. |
| WO2011091350A2 (en) * | 2010-01-25 | 2011-07-28 | Ventria Bioscience | Methods & compositions for improving protein production |
| US9133493B2 (en) * | 2011-04-21 | 2015-09-15 | Amgen Inc. | Method for culturing mammalian cells to improve recombinant protein production |
| US20130281355A1 (en) * | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
| US8956830B2 (en) * | 2013-03-14 | 2015-02-17 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
-
2014
- 2014-03-14 CA CA2903589A patent/CA2903589A1/en not_active Abandoned
- 2014-03-14 WO PCT/US2014/029758 patent/WO2014145091A1/en not_active Ceased
- 2014-03-14 RU RU2015144020A patent/RU2015144020A/ru not_active Application Discontinuation
- 2014-03-14 KR KR1020157029225A patent/KR102202476B1/ko active Active
- 2014-03-14 HK HK16101113.2A patent/HK1213179A1/zh unknown
- 2014-03-14 MX MX2015011781A patent/MX366112B/es active IP Right Grant
- 2014-03-14 CN CN201480027392.9A patent/CN105246510A/zh active Pending
- 2014-03-14 BR BR112015022529A patent/BR112015022529A2/pt not_active Application Discontinuation
- 2014-03-14 AR ARP140101052A patent/AR095348A1/es unknown
- 2014-03-14 EP EP14763258.2A patent/EP2968544A4/en not_active Withdrawn
- 2014-03-14 JP JP2016503216A patent/JP2016513478A/ja active Pending
- 2014-03-14 US US14/211,467 patent/US9441035B2/en active Active
-
2015
- 2015-09-11 US US14/852,382 patent/US20160102141A1/en not_active Abandoned
-
2016
- 2016-07-29 US US15/224,113 patent/US20170051049A1/en not_active Abandoned
-
2019
- 2019-06-18 JP JP2019112773A patent/JP2019193643A/ja active Pending
- 2019-10-18 US US16/657,844 patent/US20200291103A1/en not_active Abandoned
- 2019-11-15 US US16/685,798 patent/US20200347122A1/en not_active Abandoned
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