EA201992527A1 - Улучшенный способ крупномасштабного производства crm - Google Patents

Улучшенный способ крупномасштабного производства crm

Info

Publication number
EA201992527A1
EA201992527A1 EA201992527A EA201992527A EA201992527A1 EA 201992527 A1 EA201992527 A1 EA 201992527A1 EA 201992527 A EA201992527 A EA 201992527A EA 201992527 A EA201992527 A EA 201992527A EA 201992527 A1 EA201992527 A1 EA 201992527A1
Authority
EA
Eurasian Patent Office
Prior art keywords
crm
improved method
scale
production
gene
Prior art date
Application number
EA201992527A
Other languages
English (en)
Inventor
Баламурали Масиламани
Раджан Срираман
Мандар Шириш Диксит
Девипрасанна Чакка
Сатиям Найду Суредди
Рамеш Венкат Матур
Нарендер Дев Мантена
Махима Датла
Original Assignee
Байолоджикал И Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Байолоджикал И Лимитед filed Critical Байолоджикал И Лимитед
Publication of EA201992527A1 publication Critical patent/EA201992527A1/ru

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/385—Haptens or antigens, bound to carriers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/05—Actinobacteria, e.g. Actinomyces, Streptomyces, Nocardia, Bifidobacterium, Gardnerella, Corynebacterium; Propionibacterium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Manufacture Of Electron Tubes, Discharge Lamp Vessels, Lead-In Wires, And The Like (AREA)
  • Forging (AREA)

Abstract

Настоящее изобретение относится к улучшенному способу производства CRMс высоким выходом путем использования генно-модифицированного штамма Corynebacterium diphtheria с увеличенным количеством копий гена CRM, включающий выращивание указанного штамма в ферментативной среде без компонентов животного происхождения, содержащей одну или более аминокислот.
EA201992527A 2017-04-22 2018-04-19 Улучшенный способ крупномасштабного производства crm EA201992527A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201741014335 2017-04-22
PCT/IN2018/050235 WO2018193475A2 (en) 2017-04-22 2018-04-19 An improved method for high level production of crm

Publications (1)

Publication Number Publication Date
EA201992527A1 true EA201992527A1 (ru) 2020-02-28

Family

ID=63678648

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201992527A EA201992527A1 (ru) 2017-04-22 2018-04-19 Улучшенный способ крупномасштабного производства crm

Country Status (30)

Country Link
US (1) US11098089B2 (ru)
EP (1) EP3612553B1 (ru)
JP (1) JP7161493B2 (ru)
KR (1) KR102637436B1 (ru)
CN (1) CN110741013B (ru)
AU (1) AU2018255959B2 (ru)
BR (1) BR112019022093A2 (ru)
CA (1) CA3060723A1 (ru)
CL (1) CL2019003003A1 (ru)
CO (1) CO2019011643A2 (ru)
CU (1) CU24668B1 (ru)
DK (1) DK3612553T3 (ru)
EA (1) EA201992527A1 (ru)
ES (1) ES2926535T3 (ru)
HR (1) HRP20221060T1 (ru)
HU (1) HUE059629T2 (ru)
IL (1) IL270075B1 (ru)
LT (1) LT3612553T (ru)
MX (1) MX2019012563A (ru)
MY (1) MY194242A (ru)
PH (1) PH12019550214A1 (ru)
PL (1) PL3612553T3 (ru)
PT (1) PT3612553T (ru)
RS (1) RS63551B1 (ru)
SA (1) SA519410306B1 (ru)
SG (1) SG11201909765XA (ru)
SI (1) SI3612553T1 (ru)
UA (1) UA127497C2 (ru)
WO (1) WO2018193475A2 (ru)
ZA (1) ZA201906270B (ru)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113755380A (zh) * 2021-09-23 2021-12-07 艾美卫信生物药业（浙江）有限公司 一种发酵生产白喉毒素crm197蛋白的菌种培养基、配制方法以及菌种复苏培养方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69434079T2 (de) * 1993-03-05 2005-02-24 Wyeth Holdings Corp. Plasmid zur Herstellung von CRM-Protein und Diphtherie-Toxin
US20040087020A1 (en) * 1997-05-28 2004-05-06 Chiron S.P.A. Culture medium with yeast or soy bean extract as amino acid source and no protein complexes of animal origin
EP2199382A3 (en) 1997-05-28 2010-09-22 Novartis Vaccines and Diagnostics S.r.l. Culture medium with yeast or soy bean extract as aminoacid source and no protein complexes of animal origin
GB9904582D0 (en) 1999-02-26 1999-04-21 Nycomed Imaging As Process
EP1692269A4 (en) * 2003-12-12 2007-09-26 Sanofi Pasteur Ltd PRODUCTION OF DIPHTHERIA TOXIN
GB0505996D0 (en) * 2005-03-23 2005-04-27 Glaxosmithkline Biolog Sa Fermentation process
AU2010201410B2 (en) * 2010-03-30 2015-04-30 Pelican Technology Holdings, Inc. High level expression of recombinant CRM197
GB2495341B (en) * 2011-11-11 2013-09-18 Novartis Ag Fermentation methods and their products
FR2992656B1 (fr) * 2012-07-02 2016-10-07 Sanofi Pasteur Procede de production d'antigenes haemophilus influenzae type b
ITMI20130142A1 (it) * 2013-01-31 2014-08-01 Biosynth Srl Vaccini glicoconiugati comprendenti unita' di base di un costrutto molecolare esprimente epitopi multipli incorporati
JP6473460B2 (ja) * 2014-03-03 2019-02-20 スカラブ ゲノミクス， エルエルシー 大腸菌における組換えｃｒｍ１９７の産生強化

Also Published As

Publication number Publication date
PH12019550214A1 (en) 2020-09-14
AU2018255959B2 (en) 2022-02-17
BR112019022093A2 (pt) 2020-05-05
CA3060723A1 (en) 2018-10-25
SG11201909765XA (en) 2019-11-28
WO2018193475A3 (en) 2018-12-27
CU24668B1 (es) 2023-07-12
CU20190083A7 (es) 2020-10-20
SA519410306B1 (ar) 2022-02-02
CN110741013A (zh) 2020-01-31
KR20200010218A (ko) 2020-01-30
EP3612553A2 (en) 2020-02-26
IL270075B1 (en) 2024-02-01
UA127497C2 (uk) 2023-09-13
RS63551B1 (sr) 2022-09-30
PT3612553T (pt) 2022-09-08
AU2018255959A1 (en) 2019-11-14
WO2018193475A2 (en) 2018-10-25
ES2926535T3 (es) 2022-10-26
MX2019012563A (es) 2019-12-02
CL2019003003A1 (es) 2020-02-14
MY194242A (en) 2022-11-24
PL3612553T3 (pl) 2022-11-07
JP7161493B2 (ja) 2022-10-26
CO2019011643A2 (es) 2020-01-17
AU2018255959A8 (en) 2022-02-17
CN110741013B (zh) 2023-08-08
US20200277341A1 (en) 2020-09-03
EP3612553B1 (en) 2022-06-08
HUE059629T2 (hu) 2022-12-28
LT3612553T (lt) 2022-09-26
DK3612553T3 (da) 2022-08-29
SI3612553T1 (sl) 2022-11-30
IL270075A (ru) 2019-12-31
ZA201906270B (en) 2020-07-29
US11098089B2 (en) 2021-08-24
HRP20221060T1 (hr) 2022-11-11
KR102637436B1 (ko) 2024-02-15
JP2020517274A (ja) 2020-06-18

Similar Documents

Publication Publication Date Title
MX2023009109A (es) Una variante de isopropilmalato sintasa novedosa y un procedimiento de produccion de l-leucina usando la misma.
GB2568444A (en) De novo synthesized nucleic acid libraries
BR112015031233A2 (pt) métodos de produção de derivados de ácidos graxos omega-hidroxilados
BR112017021255A2 (pt) microrganismo modificado para a produção otimizada de 2,4-di-hidroxibutirato
MY191029A (en) Novel polypeptide and method of producing imp using the same
MY195408A (en) Novel Aspartokinase Variant and Method for Producing L- Amino Acid Using the Same
BR112013018385A2 (pt) microorganismo com produtividade de l-aminoácidos aumentada e processo de produção de l-aminoácidos usando o mesmo
EA202191822A1 (ru) Новые ацетилтрансферазы
BR112017025923A2 (pt) molécula de ácido nucleico recombinante, molécula de aminoácido recombinante, construto de expressão recombinante, vetor recombinante, micro-organismo recombinante, composição, métodos para produção de um micro-organismo recombinante e para cultivo e crescimento de um micro-organismo geneticamente modificado, uso de uma molécula de ácido nucleico recombinante, e, processo para produção fermentativa de um composto.
MY172080A (en) Microorganisms having putrescine productivity and process for producing putrescine using the same
AR079187A1 (es) Proceso de fermentacion para la produccion de acido glicolico
CL2019000318A1 (es) Método para producir un l-aminoácido tal como l-lisina.
MY181203A (en) Microorganism of corynebacterium sp. having enhanced l-lysine producibility and method for producing l-lysine using same
MY169049A (en) Microorganisms for producing putrescine and process for producing putrescine using them
PH12020551274A1 (en) Mycosporine-like amino acid-producing microorganism and method for production of mycosporine-like amino acids by using same
MY171648A (en) Microorganism producing l-lysine and method for producing l-lysine using the same
MY193587A (en) Genetically modified microorganism for improved production of fine chemicals on sucrose
MY182443A (en) Method of producing lipid
MY188407A (en) Novel lactobacillus sp. microorganisms, and composition for animal feed comprising same
EA201992527A1 (ru) Улучшенный способ крупномасштабного производства crm
PH12021550347A1 (en) MICROORGANISM FOR PRODUCING L-AMINO ACID WITH ENHANCED ACTIVITY OF a-GLUCOSIDASE AND METHOD FOR PRODUCING L-AMINO ACID USING THE SAME
MY181143A (en) Microorganisms producing l-amino acids and process for producing l-amino acids using the same
BR112016018195A2 (pt) micro-organismo modificado, método de produção de um composto orgânico e uso de um micro-organismo modificado
RU2016142706A (ru) Микроорганизм для продуцирования диамина и способ получения диамина с его использованием
RU2016141403A (ru) Микроорганизм для продуцирования диамина и способ получения диамина с его использованием