JP2015522271A5 - - Google Patents

Info

Publication number

JP2015522271A5

JP2015522271A5 JP2015519266A JP2015519266A JP2015522271A5 JP 2015522271 A5 JP2015522271 A5 JP 2015522271A5 JP 2015519266 A JP2015519266 A JP 2015519266A JP 2015519266 A JP2015519266 A JP 2015519266A JP 2015522271 A5 JP2015522271 A5 JP 2015522271A5

Authority

JP

Japan

Prior art keywords

seq

primer

reverse

pair

reverse primer

Prior art date

2013-06-27

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims description 22
• 238000006243 chemical reaction Methods 0.000 claims description 18
• 230000000741 diarrhetic effect Effects 0.000 claims description 11
• 244000052769 pathogen Species 0.000 claims description 11
• 241000589877 Campylobacter coli Species 0.000 claims description 4
• 241000589875 Campylobacter jejuni Species 0.000 claims description 4
• 241000607477 Yersinia pseudotuberculosis Species 0.000 claims description 4
• 241000224432 Entamoeba histolytica Species 0.000 claims description 2
• 241000224467 Giardia intestinalis Species 0.000 claims description 2
• 241000607142 Salmonella Species 0.000 claims description 2
• 241000607768 Shigella Species 0.000 claims description 2
• 241000607626 Vibrio cholerae Species 0.000 claims description 2
• 241000607447 Yersinia enterocolitica Species 0.000 claims description 2
• 241000607479 Yersinia pestis Species 0.000 claims description 2
• 229940007078 entamoeba histolytica Drugs 0.000 claims description 2
• 229940085435 giardia lamblia Drugs 0.000 claims description 2
• 229940118696 vibrio cholerae Drugs 0.000 claims description 2
• 229940098232 yersinia enterocolitica Drugs 0.000 claims description 2
• 239000000523 sample Substances 0.000 claims 47
• 125000003729 nucleotide group Chemical group 0.000 claims 20
• 239000002773 nucleotide Substances 0.000 claims 19
• 230000001717 pathogenic effect Effects 0.000 claims 8
• 108020004707 nucleic acids Proteins 0.000 claims 7
• 150000007523 nucleic acids Chemical class 0.000 claims 7
• 102000039446 nucleic acids Human genes 0.000 claims 7
• 238000003556 assay Methods 0.000 claims 4
• 238000007403 mPCR Methods 0.000 claims 4
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
• 108091093088 Amplicon Proteins 0.000 claims 3
• 239000012472 biological sample Substances 0.000 claims 3
• 238000001514 detection method Methods 0.000 claims 3
• 108020004711 Nucleic Acid Probes Proteins 0.000 claims 2
• 239000000203 mixture Substances 0.000 claims 2
• 239000002853 nucleic acid probe Substances 0.000 claims 2
• 238000003752 polymerase chain reaction Methods 0.000 claims 2
• 239000011541 reaction mixture Substances 0.000 claims 2
• 241000589876 Campylobacter Species 0.000 claims 1
• 241000223935 Cryptosporidium Species 0.000 claims 1
• 239000012807 PCR reagent Substances 0.000 claims 1
• 241000607734 Yersinia <bacteria> Species 0.000 claims 1
• 239000000654 additive Substances 0.000 claims 1
• 239000000872 buffer Substances 0.000 claims 1
• 238000002844 melting Methods 0.000 claims 1
• 230000008018 melting Effects 0.000 claims 1
• 239000013615 primer Substances 0.000 claims 1
• 239000002987 primer (paints) Substances 0.000 claims 1
• 238000003753 real-time PCR Methods 0.000 claims 1
• 150000003839 salts Chemical class 0.000 claims 1
• 239000004094 surface-active agent Substances 0.000 claims 1
• 241000295636 Cryptosporidium sp. Species 0.000 description 1
• 239000012139 lysis buffer Substances 0.000 description 1