CN104662167B - 确定致腹泻病原体的存在的方法 - Google Patents
确定致腹泻病原体的存在的方法 Download PDFInfo
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- CN104662167B CN104662167B CN201380041290.8A CN201380041290A CN104662167B CN 104662167 B CN104662167 B CN 104662167B CN 201380041290 A CN201380041290 A CN 201380041290A CN 104662167 B CN104662167 B CN 104662167B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261664959P | 2012-06-27 | 2012-06-27 | |
| US61/664,959 | 2012-06-27 | ||
| FI20125730 | 2012-06-27 | ||
| FI20125730 | 2012-06-27 | ||
| PCT/FI2013/050716 WO2014001648A1 (en) | 2012-06-27 | 2013-06-27 | Method for determining the presence of diarrhoea causing pathogens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104662167A CN104662167A (zh) | 2015-05-27 |
| CN104662167B true CN104662167B (zh) | 2020-06-16 |
Family
ID=49782335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380041290.8A Active CN104662167B (zh) | 2012-06-27 | 2013-06-27 | 确定致腹泻病原体的存在的方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US10724106B2 (enExample) |
| EP (1) | EP2867372B1 (enExample) |
| JP (1) | JP6498115B2 (enExample) |
| CN (1) | CN104662167B (enExample) |
| AU (1) | AU2013283152B2 (enExample) |
| CA (1) | CA2877835C (enExample) |
| PL (1) | PL2867372T3 (enExample) |
| WO (1) | WO2014001648A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2014003938A (es) * | 2014-04-01 | 2015-09-30 | Univ Nac Autónoma De México | Macroarreglos para deteccion en muestras ambientales y biologicas de microorganismos enteropatogenos. |
| US11965216B2 (en) | 2015-04-07 | 2024-04-23 | Polyskope Labs | Detection of one or more pathogens |
| JP6728657B2 (ja) * | 2015-12-04 | 2020-07-22 | 東洋紡株式会社 | 核酸増幅法 |
| CN107090518B (zh) * | 2017-04-05 | 2020-03-20 | 苏州协云基因科技有限公司 | 腹泻相关病原体多重rt-pcr联合基因芯片检测试剂盒 |
| CN109234420A (zh) * | 2018-10-09 | 2019-01-18 | 河南省奶牛生产性能测定中心 | 检测微小隐孢子虫的荧光定量pcr引物、探针、试剂盒及方法 |
| EP4359556A4 (en) * | 2021-06-21 | 2025-05-21 | The Johns Hopkins University | Rapid lamp methods for detecting bacterial and viral pathogens |
| CN113801951A (zh) * | 2021-11-02 | 2021-12-17 | 中国动物卫生与流行病学中心 | 一种用于同时检测多种致猪死亡的病原微生物的引物探针组、试剂盒及其应用 |
| CN114752594B (zh) * | 2022-05-20 | 2024-03-15 | 华中农业大学 | 用于检测沙门氏菌和隐孢子虫的多重实时荧光定量pcr引物和探针组合 |
| CN116064925B (zh) * | 2022-07-06 | 2025-12-05 | 北京博晖创新生物技术集团股份有限公司 | 一种用于胃肠道病原体检测的引物探针组合物及其应用 |
| WO2024161179A1 (en) * | 2023-01-31 | 2024-08-08 | Mobidiag Oy | Compositions and methods for detecting stx nucleic acids |
| CN119639934A (zh) * | 2025-02-18 | 2025-03-18 | 江苏硕世生物科技股份有限公司 | 一种快速检测五种致泻性大肠杆菌的探针引物组合及试剂盒 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0242334A1 (de) | 1986-04-16 | 1987-10-21 | Eduard Winner | Verfahren zur Herstellung von Beton und betonähnlichen Stoffen sowie Dach- und Fassadenbauteile |
| FR2605114B1 (fr) | 1986-10-01 | 1990-02-16 | Reosc | Lentille stigmatique a plusieurs longueurs d'onde |
| RU2329305C2 (ru) | 2001-03-01 | 2008-07-20 | Дзе Джонс Хопкинс Юниверсити | Количественный анализ, позволяющий одновременно обнаруживать и идентифицировать бактериальные инфекции |
| CA2448098A1 (en) | 2002-11-26 | 2004-05-26 | Paul A. Horgen | Ultrasensitive detection of pathogenic microbes |
| WO2005005659A1 (en) * | 2003-07-14 | 2005-01-20 | Statens Serum Institut | Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp. |
| CA2557543A1 (en) | 2004-02-27 | 2005-09-09 | Stichting Laboratorium Voor Infectieziekten | Method for detecting a microorganism in a fecal specimen |
| EP1725681A2 (en) | 2004-03-19 | 2006-11-29 | Université Catholique de Louvain | Assay for detecting and identifying micro-organisms |
| CN101351559A (zh) | 2005-11-09 | 2009-01-21 | 普里梅拉生物系统有限公司 | 病原体的多元定量检测 |
| CN101113475B (zh) | 2007-05-30 | 2010-10-06 | 中国疾病预防控制中心传染病预防控制所 | 病原微生物检测用引物和使用所述引物的多重扩增 |
| CN101113476B (zh) * | 2007-05-30 | 2010-05-26 | 中国疾病预防控制中心传染病预防控制所 | 一种病原微生物dna检测芯片及其制备方法和应用 |
| CN101113471A (zh) | 2007-06-07 | 2008-01-30 | 天津出入境检验检疫局动植物与食品检测中心 | 运用复合荧光pcr技术检测食源性致病肠杆菌的方法 |
| CN101245384A (zh) | 2008-01-25 | 2008-08-20 | 广东省微生物研究所 | 畜禽肉中沙门氏菌和大肠杆菌o157多重pcr快速检测试剂盒及检测方法 |
| CN101235410B (zh) | 2008-01-25 | 2011-01-19 | 广东省微生物研究所 | 水产品中致病菌多重pcr快速检测试剂盒及检测方法 |
| CN101760517A (zh) * | 2008-12-26 | 2010-06-30 | 天津生物芯片技术有限责任公司 | 用于检测肠产毒性大肠杆菌的基因芯片及使用方法、检测试剂盒 |
| CN101831491A (zh) | 2009-03-11 | 2010-09-15 | 北京泰格瑞分子检验有限公司 | 一种系统置换的多重基因扩增技术 |
| KR20110138289A (ko) | 2009-04-22 | 2011-12-26 | 미쓰비시 타나베 파마 코퍼레이션 | 염기 변이 판별용 프로브 세트 및 염기 변이의 판별 방법 |
| EP2485752A4 (en) | 2009-10-09 | 2013-07-31 | Univ South Dakota | VACCINES WITH ENTEROTOXY E. COLI FUSION PROTEINS |
| US20130059756A1 (en) | 2010-04-14 | 2013-03-07 | Takaaki Yamasaki | Primer set for pcr, peaction liquid for pcr, and method for detecting food poisoning bacteria |
-
2013
- 2013-06-27 AU AU2013283152A patent/AU2013283152B2/en not_active Ceased
- 2013-06-27 PL PL13809410T patent/PL2867372T3/pl unknown
- 2013-06-27 CA CA2877835A patent/CA2877835C/en active Active
- 2013-06-27 WO PCT/FI2013/050716 patent/WO2014001648A1/en not_active Ceased
- 2013-06-27 EP EP13809410.7A patent/EP2867372B1/en active Active
- 2013-06-27 JP JP2015519266A patent/JP6498115B2/ja active Active
- 2013-06-27 CN CN201380041290.8A patent/CN104662167B/zh active Active
- 2013-06-27 US US14/411,170 patent/US10724106B2/en active Active
Non-Patent Citations (3)
| Title |
|---|
| Duplex Real-Time SYBR Green PCR Assays for Detection of 17 Species of Food- or Waterborne Pathogens in Stools;Fukushima Hiroshi et al.;《JOURNAL OF CLINICAL MICROBIOLOGY》;20031130;第41卷(第11期);全文 * |
| Rapid detection of Escherichia coli virulence factor genes using multiplex real-time TaqMan PCR assays;West D.M. et al.;《Veterinary Microbiology》;20071231;第122卷;全文 * |
| Taqman三重实时PCR快速检测原料乳中致泻性大肠埃希氏菌;张红宇等;《东北农业大学学报》;20101031;第41卷(第10期);摘要、第1.3.2、1.3.3、2.1节、表1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015522271A (ja) | 2015-08-06 |
| EP2867372A4 (en) | 2016-04-13 |
| WO2014001648A1 (en) | 2014-01-03 |
| CN104662167A (zh) | 2015-05-27 |
| US10724106B2 (en) | 2020-07-28 |
| US20150299774A1 (en) | 2015-10-22 |
| JP6498115B2 (ja) | 2019-04-10 |
| CA2877835C (en) | 2021-11-16 |
| AU2013283152B2 (en) | 2019-04-18 |
| AU2013283152A1 (en) | 2015-02-12 |
| EP2867372A1 (en) | 2015-05-06 |
| CA2877835A1 (en) | 2014-01-03 |
| EP2867372B1 (en) | 2018-12-19 |
| PL2867372T3 (pl) | 2019-06-28 |
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