CA2877835C - Method for determining the presence of diarrhoea causing pathogens - Google Patents

Method for determining the presence of diarrhoea causing pathogens Download PDF

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Publication number
CA2877835C
CA2877835C CA2877835A CA2877835A CA2877835C CA 2877835 C CA2877835 C CA 2877835C CA 2877835 A CA2877835 A CA 2877835A CA 2877835 A CA2877835 A CA 2877835A CA 2877835 C CA2877835 C CA 2877835C
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seq
primer
ffl
reverse
reverse primer
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French (fr)
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CA2877835A1 (en
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Jenni ANTIKAINEN
Juha Kirveskari
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Mobidiag Oy
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Mobidiag Oy
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2877835A 2012-06-27 2013-06-27 Method for determining the presence of diarrhoea causing pathogens Active CA2877835C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261664959P 2012-06-27 2012-06-27
FI20125730 2012-06-27
US61/664,959 2012-06-27
FI20125730 2012-06-27
PCT/FI2013/050716 WO2014001648A1 (en) 2012-06-27 2013-06-27 Method for determining the presence of diarrhoea causing pathogens

Publications (2)

Publication Number Publication Date
CA2877835A1 CA2877835A1 (en) 2014-01-03
CA2877835C true CA2877835C (en) 2021-11-16

Family

ID=49782335

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2877835A Active CA2877835C (en) 2012-06-27 2013-06-27 Method for determining the presence of diarrhoea causing pathogens

Country Status (8)

Country Link
US (1) US10724106B2 (enExample)
EP (1) EP2867372B1 (enExample)
JP (1) JP6498115B2 (enExample)
CN (1) CN104662167B (enExample)
AU (1) AU2013283152B2 (enExample)
CA (1) CA2877835C (enExample)
PL (1) PL2867372T3 (enExample)
WO (1) WO2014001648A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2014003938A (es) * 2014-04-01 2015-09-30 Univ Nac Autónoma De México Macroarreglos para deteccion en muestras ambientales y biologicas de microorganismos enteropatogenos.
HK1251023A1 (zh) 2015-04-07 2019-01-18 Polyskope Labs 检测一个或多个病原体
JP6728657B2 (ja) * 2015-12-04 2020-07-22 東洋紡株式会社 核酸増幅法
CN107090518B (zh) * 2017-04-05 2020-03-20 苏州协云基因科技有限公司 腹泻相关病原体多重rt-pcr联合基因芯片检测试剂盒
CN109234420A (zh) * 2018-10-09 2019-01-18 河南省奶牛生产性能测定中心 检测微小隐孢子虫的荧光定量pcr引物、探针、试剂盒及方法
WO2022271743A1 (en) * 2021-06-21 2022-12-29 The Johns Hopkins University Rapid lamp methods for detecting bacterial and viral pathogens
CN113801951A (zh) * 2021-11-02 2021-12-17 中国动物卫生与流行病学中心 一种用于同时检测多种致猪死亡的病原微生物的引物探针组、试剂盒及其应用
CN114752594B (zh) * 2022-05-20 2024-03-15 华中农业大学 用于检测沙门氏菌和隐孢子虫的多重实时荧光定量pcr引物和探针组合
CN116064925B (zh) * 2022-07-06 2025-12-05 北京博晖创新生物技术集团股份有限公司 一种用于胃肠道病原体检测的引物探针组合物及其应用
WO2024161179A1 (en) * 2023-01-31 2024-08-08 Mobidiag Oy Compositions and methods for detecting stx nucleic acids
CN119639934A (zh) * 2025-02-18 2025-03-18 江苏硕世生物科技股份有限公司 一种快速检测五种致泻性大肠杆菌的探针引物组合及试剂盒

Family Cites Families (18)

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EP0242334A1 (de) 1986-04-16 1987-10-21 Eduard Winner Verfahren zur Herstellung von Beton und betonähnlichen Stoffen sowie Dach- und Fassadenbauteile
FR2605114B1 (fr) 1986-10-01 1990-02-16 Reosc Lentille stigmatique a plusieurs longueurs d'onde
RU2329305C2 (ru) 2001-03-01 2008-07-20 Дзе Джонс Хопкинс Юниверсити Количественный анализ, позволяющий одновременно обнаруживать и идентифицировать бактериальные инфекции
US20040248148A1 (en) 2002-11-26 2004-12-09 Horgen Paul A. Ultrasensitive detection of pathogenic microbes
US20060194206A1 (en) 2003-07-14 2006-08-31 Soren Persson Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp
CA2557543A1 (en) 2004-02-27 2005-09-09 Stichting Laboratorium Voor Infectieziekten Method for detecting a microorganism in a fecal specimen
US7785780B2 (en) 2004-03-19 2010-08-31 Universite Catholique De Louvain Assay for detecting and identifying micro-organisms
EP1951888B1 (en) 2005-11-09 2012-09-05 PrimeraDx, Inc. Multiplexed quantitative detection of pathogens
CN101113476B (zh) * 2007-05-30 2010-05-26 中国疾病预防控制中心传染病预防控制所 一种病原微生物dna检测芯片及其制备方法和应用
CN101113475B (zh) * 2007-05-30 2010-10-06 中国疾病预防控制中心传染病预防控制所 病原微生物检测用引物和使用所述引物的多重扩增
CN101113471A (zh) 2007-06-07 2008-01-30 天津出入境检验检疫局动植物与食品检测中心 运用复合荧光pcr技术检测食源性致病肠杆菌的方法
CN101235410B (zh) 2008-01-25 2011-01-19 广东省微生物研究所 水产品中致病菌多重pcr快速检测试剂盒及检测方法
CN101245384A (zh) 2008-01-25 2008-08-20 广东省微生物研究所 畜禽肉中沙门氏菌和大肠杆菌o157多重pcr快速检测试剂盒及检测方法
CN101760517A (zh) 2008-12-26 2010-06-30 天津生物芯片技术有限责任公司 用于检测肠产毒性大肠杆菌的基因芯片及使用方法、检测试剂盒
CN101831491A (zh) * 2009-03-11 2010-09-15 北京泰格瑞分子检验有限公司 一种系统置换的多重基因扩增技术
MX2011011040A (es) * 2009-04-22 2011-11-04 Vertex Pharma Grupo de sondas para identificar la mutacion del nucleotido y metodo para identificar la mutacion del nucleotido.
EP2485752A4 (en) 2009-10-09 2013-07-31 Univ South Dakota VACCINES WITH ENTEROTOXY E. COLI FUSION PROTEINS
JP5830461B2 (ja) 2010-04-14 2015-12-09 東洋製罐グループホールディングス株式会社 Pcr用プライマーセット、pcr用反応液、食中毒菌の検出方法

Also Published As

Publication number Publication date
PL2867372T3 (pl) 2019-06-28
US20150299774A1 (en) 2015-10-22
EP2867372A4 (en) 2016-04-13
EP2867372A1 (en) 2015-05-06
AU2013283152B2 (en) 2019-04-18
EP2867372B1 (en) 2018-12-19
AU2013283152A1 (en) 2015-02-12
CA2877835A1 (en) 2014-01-03
WO2014001648A1 (en) 2014-01-03
JP2015522271A (ja) 2015-08-06
CN104662167A (zh) 2015-05-27
US10724106B2 (en) 2020-07-28
CN104662167B (zh) 2020-06-16
JP6498115B2 (ja) 2019-04-10

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