JP2010501179A5 - - Google Patents

Info

Publication number

JP2010501179A5

JP2010501179A5 JP2009525579A JP2009525579A JP2010501179A5 JP 2010501179 A5 JP2010501179 A5 JP 2010501179A5 JP 2009525579 A JP2009525579 A JP 2009525579A JP 2009525579 A JP2009525579 A JP 2009525579A JP 2010501179 A5 JP2010501179 A5 JP 2010501179A5

Authority

JP

Japan

Prior art keywords

wssv

seq

nucleic acid

isolated

diagnostic primer

Prior art date

2007-08-17

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

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• 241000318927 Shrimp white spot syndrome virus Species 0.000 claims description 60
• 239000000523 sample Substances 0.000 claims description 39
• 150000007523 nucleic acids Chemical class 0.000 claims description 31
• 108020004707 nucleic acids Proteins 0.000 claims description 29
• 102000039446 nucleic acids Human genes 0.000 claims description 29
• 238000000034 method Methods 0.000 claims description 25
• 230000000295 complement effect Effects 0.000 claims description 19
• 230000003321 amplification Effects 0.000 claims description 12
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 12
• 239000003795 chemical substances by application Substances 0.000 claims description 7
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 4
• 238000005382 thermal cycling Methods 0.000 claims description 4
• 238000012790 confirmation Methods 0.000 claims description 2
• 238000004925 denaturation Methods 0.000 claims description 2
• 230000036425 denaturation Effects 0.000 claims description 2
• 238000003745 diagnosis Methods 0.000 claims description 2
• 238000009396 hybridization Methods 0.000 claims description 2
• 239000012086 standard solution Substances 0.000 claims description 2
• 108020004414 DNA Proteins 0.000 description 6
• 108091093088 Amplicon Proteins 0.000 description 2
• 239000003153 chemical reaction reagent Substances 0.000 description 2
• 230000002401 inhibitory effect Effects 0.000 description 2
• 230000010076 replication Effects 0.000 description 2
• KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 1
• 230000005778 DNA damage Effects 0.000 description 1
• 231100000277 DNA damage Toxicity 0.000 description 1
• 241000238557 Decapoda Species 0.000 description 1
• 108091028043 Nucleic acid sequence Proteins 0.000 description 1
• 230000015572 biosynthetic process Effects 0.000 description 1
• 239000013043 chemical agent Substances 0.000 description 1
• OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 1
• OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 1
• 238000001514 detection method Methods 0.000 description 1
• 239000005546 dideoxynucleotide Substances 0.000 description 1
• 238000011156 evaluation Methods 0.000 description 1
• 238000001917 fluorescence detection Methods 0.000 description 1
• 230000036541 health Effects 0.000 description 1
• 230000002779 inactivation Effects 0.000 description 1
• 239000000203 mixture Substances 0.000 description 1
• 239000002777 nucleoside Substances 0.000 description 1
• 125000003835 nucleoside group Chemical group 0.000 description 1
• UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical group [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
• 238000003752 polymerase chain reaction Methods 0.000 description 1
• 239000000126 substance Substances 0.000 description 1
• 239000001226 triphosphate Substances 0.000 description 1
• 235000011178 triphosphate Nutrition 0.000 description 1
• 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1