JP2014083053A5 - - Google Patents

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Publication number
JP2014083053A5
JP2014083053A5 JP2013214714A JP2013214714A JP2014083053A5 JP 2014083053 A5 JP2014083053 A5 JP 2014083053A5 JP 2013214714 A JP2013214714 A JP 2013214714A JP 2013214714 A JP2013214714 A JP 2013214714A JP 2014083053 A5 JP2014083053 A5 JP 2014083053A5
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JP
Japan
Prior art keywords
seq
poly
nucleic acid
primer
sequence
Prior art date
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Application number
JP2013214714A
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English (en)
Japanese (ja)
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JP2014083053A (ja
JP6659206B2 (ja
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Publication date
Priority claimed from EP12189009.9A external-priority patent/EP2722399A1/en
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Publication of JP2014083053A publication Critical patent/JP2014083053A/ja
Publication of JP2014083053A5 publication Critical patent/JP2014083053A5/ja
Application granted granted Critical
Publication of JP6659206B2 publication Critical patent/JP6659206B2/ja
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JP2013214714A 2012-10-18 2013-10-15 増幅中の高分子量生成物を防止する方法 Active JP6659206B2 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261715464P 2012-10-18 2012-10-18
EP12189009.9 2012-10-18
EP12189009.9A EP2722399A1 (en) 2012-10-18 2012-10-18 Method for preventing high molecular weight products during amplification
US61/715464 2012-10-18

Publications (3)

Publication Number Publication Date
JP2014083053A JP2014083053A (ja) 2014-05-12
JP2014083053A5 true JP2014083053A5 (enExample) 2016-10-13
JP6659206B2 JP6659206B2 (ja) 2020-03-04

Family

ID=47040587

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013214714A Active JP6659206B2 (ja) 2012-10-18 2013-10-15 増幅中の高分子量生成物を防止する方法

Country Status (5)

Country Link
US (1) US9447476B2 (enExample)
EP (2) EP2722399A1 (enExample)
JP (1) JP6659206B2 (enExample)
CN (1) CN103773841B (enExample)
ES (1) ES2628286T3 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2722399A1 (en) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
CA2896616C (en) * 2013-01-10 2018-03-13 F. Hoffmann-La Roche Ag Improved calibration of high resolution melting
WO2014160254A2 (en) * 2013-03-14 2014-10-02 Abbott Molecular Inc. Minimizing errors using uracil-dna-n-glycosylase
CN107075585B (zh) 2014-10-14 2021-07-20 雅培实验室 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法
ES2791280T3 (es) 2014-12-24 2020-11-03 Abbott Lab Método de detección, composición y kit basados en reacciones en cascada de ADN amplificador de señal con extensión de la diana
JP6240632B2 (ja) * 2015-04-03 2017-11-29 株式会社スタックシステム 殺菌消毒液の製造方法
CN105400908B (zh) * 2015-12-30 2019-04-05 中国检验检疫科学研究院 一种利用焦磷酸测序技术检测斑点叉尾鮰病毒的引物、试剂盒和检测方法
US10655190B2 (en) * 2016-03-11 2020-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Zika virus
WO2017212023A1 (en) * 2016-06-10 2017-12-14 Roche Diagnostics Gmbh Compositions and methods for detection of hepatitis c virus genotype 3
CN109642253A (zh) 2016-08-02 2019-04-16 豪夫迈·罗氏有限公司 用于提高核酸的扩增和检测/定量的效率的辅助寡核苷酸
JP6789866B2 (ja) * 2017-03-28 2020-11-25 京セラ株式会社 蓄電装置及び電力管理システム
CN109694926A (zh) * 2018-12-29 2019-04-30 中山大学达安基因股份有限公司 一种定量检测hbv核酸的方法及试剂盒

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US6716580B2 (en) * 1990-06-11 2004-04-06 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
EP0728218B1 (en) 1993-11-12 2008-01-02 PHRI Properties, Inc. Hybridization probes for nucleic acid detection, universal stems, methods and kits
DE19545320A1 (de) 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
GB9600384D0 (en) 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
EP1442794B1 (en) 1996-06-04 2006-03-29 University Of Utah Research Foundation System and method for carrying out and monitoring biological processes
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
DK0866071T3 (da) 1997-03-20 2005-01-17 Hoffmann La Roche Modificerede primere
WO2000017401A2 (en) * 1998-09-21 2000-03-30 Ramot University Authority For Applied Research & Industrial Development Ltd. Method and apparatus for effecting and monitoring nucleic acid amplification
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
ATE312942T1 (de) 2000-10-25 2005-12-15 Hoffmann La Roche Amplifizierung unter verwendung von modifizierten primern
JP2003199568A (ja) 2002-01-10 2003-07-15 Nichirei Corp Dna増幅反応の効率向上方法
US7718403B2 (en) * 2003-03-07 2010-05-18 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US8268982B2 (en) * 2004-01-07 2012-09-18 Hitachi Chemical Co., Ltd. Primers and probes for the detection of HIV
US20070048758A1 (en) 2005-06-09 2007-03-01 Epoch Biosciences, Inc. Improved primer-based amplification methods
WO2008064687A1 (en) * 2006-11-27 2008-06-05 Fluimedix Fidelity enhanced allele specific amplification method
US20090291475A1 (en) 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
WO2012032510A1 (en) * 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
EP2722399A1 (en) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification

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