JP2015071629A5 - - Google Patents
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- Publication number
- JP2015071629A5 JP2015071629A5 JP2014246058A JP2014246058A JP2015071629A5 JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5 JP 2014246058 A JP2014246058 A JP 2014246058A JP 2014246058 A JP2014246058 A JP 2014246058A JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5
- Authority
- JP
- Japan
- Prior art keywords
- eluate
- sample
- flow
- protein
- hcp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 20
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 14
- 102000004169 proteins and genes Human genes 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 11
- 239000002609 medium Substances 0.000 claims 7
- 238000005349 anion exchange Methods 0.000 claims 4
- 239000003575 carbonaceous material Substances 0.000 claims 4
- 239000002775 capsule Substances 0.000 claims 2
- 238000004587 chromatography analysis Methods 0.000 claims 2
- 239000012501 chromatography medium Substances 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 238000011100 viral filtration Methods 0.000 claims 2
- 239000006096 absorbing agent Substances 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 230000003068 static effect Effects 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161575349P | 2011-08-19 | 2011-08-19 | |
| US61/575,349 | 2011-08-19 | ||
| US201261666240P | 2012-06-29 | 2012-06-29 | |
| US61/666,240 | 2012-06-29 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Division JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015071629A JP2015071629A (ja) | 2015-04-16 |
| JP2015071629A5 true JP2015071629A5 (enExample) | 2016-03-31 |
| JP6254072B2 JP6254072B2 (ja) | 2017-12-27 |
Family
ID=47076067
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Active JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2014246058A Active JP6254072B2 (ja) | 2011-08-19 | 2014-12-04 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2016022509A Active JP6339120B2 (ja) | 2011-08-19 | 2016-02-09 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2018017028A Active JP6743074B2 (ja) | 2011-08-19 | 2018-02-02 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Active JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016022509A Active JP6339120B2 (ja) | 2011-08-19 | 2016-02-09 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2018017028A Active JP6743074B2 (ja) | 2011-08-19 | 2018-02-02 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US9096648B2 (enExample) |
| EP (2) | EP2942353B1 (enExample) |
| JP (4) | JP5938295B2 (enExample) |
| KR (1) | KR101484659B1 (enExample) |
| CN (3) | CN111423488B (enExample) |
| ES (2) | ES2539232T3 (enExample) |
| TW (1) | TWI574975B (enExample) |
| WO (1) | WO2013028330A2 (enExample) |
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| US10507409B2 (en) * | 2016-03-12 | 2019-12-17 | Spf Technologies, Llc | Hyper-productive chromatography system and process |
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| WO2013028330A2 (en) * | 2011-08-19 | 2013-02-28 | Emd Millipore Corporation | Methods of reducing level of one of more impurities in a sample during protein purification |
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| EP2682168A1 (en) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| CN104411820B (zh) * | 2012-06-29 | 2017-05-03 | Emd密理博公司 | 在蛋白纯化过程中灭活病毒的方法 |
| US9650411B2 (en) * | 2012-08-07 | 2017-05-16 | Kyowa Hakko Kirin Co., Ltd. | Method of purifying protein |
| WO2014133741A1 (en) * | 2013-02-26 | 2014-09-04 | Emd Millipore Corporation | Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions |
| EP2968494B1 (en) * | 2013-03-13 | 2018-11-14 | Seattle Genetics, Inc. | Activated carbon filtration for purification of benzodiazepine adcs |
| HUE038741T2 (hu) | 2013-05-06 | 2018-11-28 | Sanofi Sa | Folyamatos többlépéses eljárás antitestek tisztítására |
| CA2910770C (en) * | 2013-07-12 | 2018-02-27 | Merck Patent Gmbh | Removal of fragments from a sample containing a target protein using activated carbon |
| KR101894762B1 (ko) * | 2013-07-12 | 2018-09-05 | 이엠디 밀리포어 코포레이션 | 표적 단백질을 함유하는 샘플로부터 활성탄을 사용하여 바이러스 제거를 결정하는 방법 |
| CN105722532A (zh) * | 2013-09-13 | 2016-06-29 | 豪夫迈·罗氏有限公司 | 包含纯化的重组多肽的方法和组合物 |
| CN103497248B (zh) * | 2013-09-22 | 2016-08-10 | 中国抗体制药有限公司 | 一种从细胞培养上清中分离纯化抗体的方法 |
| EP2986307A4 (en) | 2014-01-03 | 2017-04-12 | Bio-rad Laboratories, Inc. | Removal of impurities from protein a eluates |
| DK3114455T3 (da) * | 2014-03-04 | 2020-04-06 | Merck Patent Gmbh | Robust antistof-oprensning |
| EP3116645B1 (en) * | 2014-03-14 | 2019-04-10 | GE Healthcare BioProcess R&D AB | Separation matrices for purification of biological particles |
| EP3215246B1 (en) | 2014-11-06 | 2024-08-07 | Merck Patent GmbH | A method for the removal of leachables and/or extractables |
| JP6894843B2 (ja) * | 2015-03-13 | 2021-06-30 | ノビミューン エスアー | 二重特異性抗体を精製する方法 |
| EP3334747B1 (en) * | 2015-08-13 | 2023-09-27 | Amgen Inc. | Charged depth filtration of antigen-binding proteins |
| KR20180048731A (ko) * | 2015-08-20 | 2018-05-10 | 제넨테크, 인크. | 재조합 폴리펩티드를 제조하기 위한 fkpa의 정제 및 그의 용도 |
| US12409440B1 (en) | 2016-01-26 | 2025-09-09 | Spf Technologies Llc | Separation module |
| EP3430072B1 (en) * | 2016-03-17 | 2020-07-01 | Merck Patent GmbH | Method for purifying poloxamers |
| EP3436473A4 (en) * | 2016-03-29 | 2019-10-23 | Navya Biologicals Pvt. Ltd. | METHOD FOR PURIFYING FC FUSION PROTEINS |
| BR112018068803A2 (pt) * | 2016-04-01 | 2019-01-22 | Ucb Biopharma Sprl | método para purificação de proteína |
| CN109964123A (zh) * | 2016-11-18 | 2019-07-02 | 捷恩智株式会社 | 抗体的纯化方法 |
| WO2018183971A1 (en) * | 2017-04-01 | 2018-10-04 | Massachusetts Institute Of Technology | Systems and methods for manufacturing biologically-produced products |
| US20200247911A1 (en) * | 2017-05-17 | 2020-08-06 | Msd Wellcome Trust Hilleman Laboratories Pvt. Ltd. | Purification of Bacterial Polysaccharides |
| CN107236038B (zh) * | 2017-06-20 | 2020-11-27 | 华兰生物工程股份有限公司 | 微晶纤维素提高蛋白质澄清度的方法及其产品和应用 |
| US11697670B2 (en) | 2017-08-22 | 2023-07-11 | Biogen Ma Inc. | Methods for purifying antibodies having reduced high molecular weight aggregates |
| AU2018404665A1 (en) | 2017-08-31 | 2020-02-27 | Massachusetts Institute Of Technology | Hollow fibre membrane filtration systems and methods for manufacturing biologically-produced products |
| US11071927B2 (en) * | 2017-11-15 | 2021-07-27 | Arup Laboratories, Inc. | Separating and quantifying unbound target analytes from biological samples |
| CN109929038B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | Vegf捕获剂融合蛋白的纯化方法 |
| CN111818980B (zh) * | 2018-03-08 | 2022-10-11 | 生物辐射实验室股份有限公司 | 阴离子交换-疏水混合模式色谱树脂 |
| EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| EP3810650A1 (en) * | 2018-06-19 | 2021-04-28 | Bristol-Myers Squibb Company | Methods of purifying proteins using chromatography |
| EP3829733B1 (en) * | 2018-07-27 | 2024-12-18 | Waters Technologies Corporation | Liquid chromatography/mass spectrometry methods for the analysis of polar molecules |
| WO2020036869A1 (en) | 2018-08-16 | 2020-02-20 | Emd Millipore Corporation | Closed bioprocessing device |
| CN111215027A (zh) * | 2018-11-26 | 2020-06-02 | 中国科学院大连化学物理研究所 | 一种水热碳微球色谱介质的修饰方法 |
| US20220041650A1 (en) * | 2018-12-03 | 2022-02-10 | Duke University | Purification method for recombinant proteins and nanoparticles |
| KR102120675B1 (ko) * | 2018-12-05 | 2020-06-09 | 유바이오주식회사 | 바이러스 클리어런스 공정을 포함하는 봉독의 정제방법 및 이를 이용한 염증성 질환 예방 또는 치료용 조성물 |
| WO2020123714A1 (en) | 2018-12-12 | 2020-06-18 | Purilogics, LLC | Affinity membrane and method of preparation |
| TW202045527A (zh) * | 2019-01-23 | 2020-12-16 | 日商第一三共股份有限公司 | 包含使用活性碳材料之步驟之抗體純化方法 |
| EP3947694A4 (en) * | 2019-04-02 | 2023-01-04 | Council of Scientific & Industrial Research | CLONING AND EXPRESSION OF AN IN VIVO REFOLDED ANTIBODY FRAGMENT |
| EP3962924A1 (en) | 2019-05-03 | 2022-03-09 | Genentech, Inc. | Methods of reducing the enzymatic hydrolysis activity rate in a composition obtained from a purification platform |
| CN114040815B (zh) | 2019-09-05 | 2024-06-28 | 生物辐射实验室股份有限公司 | 阴离子交换-疏水混合模式色谱树脂 |
| JP2022548197A (ja) | 2019-12-06 | 2022-11-17 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 抗vegfタンパク質組成物及びその製造方法 |
| US20210178334A1 (en) * | 2019-12-16 | 2021-06-17 | Dionex Corporation | Electrolytic eluent generators with stabilized operating voltages |
| JP2023521193A (ja) * | 2020-04-13 | 2023-05-23 | スリーエム イノベイティブ プロパティズ カンパニー | フロースループロセス及び標的分子を精製するためのデバイス |
| CN115803009A (zh) | 2020-05-08 | 2023-03-14 | 瑞泽恩制药公司 | 用于治疗眼病和癌症的vegf阱和微阱及方法 |
| EP4237116A1 (en) * | 2020-10-30 | 2023-09-06 | Genentech, Inc. | Purification platforms for obtaining pharmaceutical compositions having a reduced hydrolytic enzyme activity rate |
| JP2023549938A (ja) * | 2020-11-20 | 2023-11-29 | アレス トレーディング ソシエテ アノニム | タンパク質を精製するための緩衝液と方法 |
| EP4271706A4 (en) * | 2020-12-28 | 2025-01-15 | Plasma Technologies LLC | SYSTEMS AND METHODS FOR THE ISOLATION OF IMMUNGLOBULIN G ON A PROCESS SCALE |
| CN112870979B (zh) * | 2020-12-31 | 2022-12-23 | 内蒙古金达威药业有限公司 | 一种β-烟酰胺单核苷酸的分离纯化方法 |
| CN115326937B (zh) * | 2021-05-11 | 2024-06-18 | 山东省食品药品检验研究院 | 一种基因毒杂质捕捉用固相探针及其使用方法与应用 |
| CA3236213A1 (en) | 2021-10-27 | 2023-05-04 | Eugene ZURLO | Compositions and methods for isolating proteins |
| CN116120392B (zh) * | 2023-04-18 | 2023-08-01 | 上海健士拜生物科技有限公司 | 聚体蛋白的纯化方法 |
| CN118126110B (zh) * | 2024-05-10 | 2024-08-06 | 江苏赛孚士生物技术有限公司 | 一种纯化单克隆抗体的方法 |
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| SG10201701224UA (en) * | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| EP2682168A1 (en) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| CA2910770C (en) * | 2013-07-12 | 2018-02-27 | Merck Patent Gmbh | Removal of fragments from a sample containing a target protein using activated carbon |
-
2012
- 2012-08-02 WO PCT/US2012/049351 patent/WO2013028330A2/en not_active Ceased
- 2012-08-02 US US13/565,463 patent/US9096648B2/en active Active
- 2012-08-07 TW TW101128407A patent/TWI574975B/zh active
- 2012-08-09 EP EP15159525.3A patent/EP2942353B1/en active Active
- 2012-08-09 ES ES12179861.5T patent/ES2539232T3/es active Active
- 2012-08-09 ES ES15159525.3T patent/ES2677650T3/es active Active
- 2012-08-09 EP EP12179861.5A patent/EP2574618B1/en active Active
- 2012-08-13 JP JP2012179197A patent/JP5938295B2/ja active Active
- 2012-08-16 KR KR20120089449A patent/KR101484659B1/ko active Active
- 2012-08-20 CN CN202010257856.2A patent/CN111423488B/zh active Active
- 2012-08-20 CN CN201210398359.XA patent/CN102977182B/zh active Active
- 2012-08-20 CN CN201610090272.4A patent/CN105669829B/zh active Active
-
2014
- 2014-12-04 JP JP2014246058A patent/JP6254072B2/ja active Active
-
2015
- 2015-06-23 US US14/747,029 patent/US10287314B2/en active Active
-
2016
- 2016-02-09 JP JP2016022509A patent/JP6339120B2/ja active Active
-
2018
- 2018-02-02 JP JP2018017028A patent/JP6743074B2/ja active Active
-
2019
- 2019-03-20 US US16/358,845 patent/US11634457B2/en active Active
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