JP2015071629A5 - - Google Patents

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JP2015071629A5
JP2015071629A5 JP2014246058A JP2014246058A JP2015071629A5 JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5 JP 2014246058 A JP2014246058 A JP 2014246058A JP 2014246058 A JP2014246058 A JP 2014246058A JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5
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protein
hcp
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JP2015071629A (ja
JP6254072B2 (ja
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JP2014246058A 2011-08-19 2014-12-04 タンパク質精製中に試料中の１または複数の不純物のレベルを低下させる方法 Active JP6254072B2 (ja)

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US201161575349P 2011-08-19 2011-08-19
US61/575,349 2011-08-19
US201261666240P 2012-06-29 2012-06-29
US61/666,240 2012-06-29

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JP2012179197A Division JP5938295B2 (ja) 2011-08-19 2012-08-13 タンパク質精製中に試料中の１または複数の不純物のレベルを低下させる方法

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JP2015071629A JP2015071629A (ja) 2015-04-16
JP2015071629A5 true JP2015071629A5 (enExample) 2016-03-31
JP6254072B2 JP6254072B2 (ja) 2017-12-27

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JP2012179197A Active JP5938295B2 (ja) 2011-08-19 2012-08-13 タンパク質精製中に試料中の１または複数の不純物のレベルを低下させる方法
JP2014246058A Active JP6254072B2 (ja) 2011-08-19 2014-12-04 タンパク質精製中に試料中の１または複数の不純物のレベルを低下させる方法
JP2016022509A Active JP6339120B2 (ja) 2011-08-19 2016-02-09 タンパク質精製中に試料中の１または複数の不純物のレベルを低下させる方法
JP2018017028A Active JP6743074B2 (ja) 2011-08-19 2018-02-02 タンパク質精製中に試料中の１または複数の不純物のレベルを低下させる方法

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JP2018017028A Active JP6743074B2 (ja) 2011-08-19 2018-02-02 タンパク質精製中に試料中の１または複数の不純物のレベルを低下させる方法

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US (3) US9096648B2 (enExample)
EP (2) EP2574618B1 (enExample)
JP (4) JP5938295B2 (enExample)
KR (1) KR101484659B1 (enExample)
CN (3) CN102977182B (enExample)
ES (2) ES2677650T3 (enExample)
TW (1) TWI574975B (enExample)
WO (1) WO2013028330A2 (enExample)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10507409B2 (en) * 2016-03-12 2019-12-17 Spf Technologies, Llc Hyper-productive chromatography system and process
US11395980B2 (en) 2010-01-25 2022-07-26 Spf Technologies Llc Chromatographic cassette
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
SG11201406407TA (en) * 2012-06-29 2014-11-27 Emd Millipore Corp Methods for inactivating viruses during a protein purification process
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9650411B2 (en) * 2012-08-07 2017-05-16 Kyowa Hakko Kirin Co., Ltd. Method of purifying protein
EP2961760B1 (en) * 2013-02-26 2021-03-24 EMD Millipore Corporation Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions
EP3441072B1 (en) * 2013-03-13 2020-12-30 Seagen Inc. Activated carbon filtration for purification of benzodiazepine adcs
ES2665645T3 (es) 2013-05-06 2018-04-26 Sanofi Proceso multietapa continuo para purificar anticuerpos
SG11201508835YA (en) * 2013-07-12 2015-11-27 Merck Patent Gmbh Removal of fragments from a sample containing a target protein using activated carbon
CN105339388B (zh) * 2013-07-12 2021-06-15 Emd密理博公司 确定利用活性炭从包含靶蛋白的样品去除病毒的方法
SG10201802525QA (en) * 2013-09-13 2018-04-27 Genentech Inc Methods and compositions comprising purified recombinant polypeptides
CN103497248B (zh) * 2013-09-22 2016-08-10 中国抗体制药有限公司 一种从细胞培养上清中分离纯化抗体的方法
CN105407905B (zh) 2014-01-03 2020-03-03 生物辐射实验室股份有限公司 蛋白a洗脱产物中杂质的去除
EP3114455B1 (en) * 2014-03-04 2020-01-15 Merck Patent GmbH Robust antibody purification
CN114917884A (zh) 2014-03-14 2022-08-19 思拓凡生物工艺研发有限公司 用于生物颗粒纯化的分离基质
KR102398719B1 (ko) * 2014-11-06 2022-05-16 메르크 파텐트 게엠베하 침출물 및/또는 추출물 제거용 활성탄
WO2016146594A1 (en) * 2015-03-13 2016-09-22 Novimmune Sa Methods of purifying bispecific antibodies
MA42613A (fr) 2015-08-13 2018-06-20 Amgen Inc Filtration en profondeur chargée de protéines de liaison à un antigène
JP7091238B2 (ja) * 2015-08-20 2022-06-27 ジェネンテック， インコーポレイテッド Ｆｋｐａの精製、及び組換えポリペプチドの産生のためのその使用
US12409440B1 (en) 2016-01-26 2025-09-09 Spf Technologies Llc Separation module
WO2017157505A1 (en) * 2016-03-17 2017-09-21 Merck Patent Gmbh Method for purifying poloxamers
WO2017168296A1 (en) * 2016-03-29 2017-10-05 Navya Biologicals Pvt. Ltd A process for purification of fc-fusion proteins
CN108883345B (zh) 2016-04-01 2022-09-13 Ucb生物制药私人有限公司 用于蛋白纯化的方法
CN109964123A (zh) * 2016-11-18 2019-07-02 捷恩智株式会社 抗体的纯化方法
US20200224144A1 (en) * 2017-04-01 2020-07-16 Massachusetts Institute Of Technology Systems and methods for manufacturing biologically-produced products
RU2019140392A (ru) * 2017-05-17 2021-06-17 Мсд Веллком Траст Хиллеман Лабораторис Пвт. Лтд. Очистка бактериальных полисахаридов
CN107236038B (zh) * 2017-06-20 2020-11-27 华兰生物工程股份有限公司 微晶纤维素提高蛋白质澄清度的方法及其产品和应用
WO2019040671A1 (en) * 2017-08-22 2019-02-28 Biogen Ma Inc. METHODS OF PURIFYING ANTIBODIES HAVING REDUCED AGGREGATES OF HIGH MOLECULAR WEIGHT
KR20200042532A (ko) 2017-08-31 2020-04-23 메사추세츠 인스티튜트 오브 테크놀로지 생물학적으로 생성된 생성물을 제조하기 위한 중공 섬유 멤브레인 여과 시스템 및 방법
US11071927B2 (en) * 2017-11-15 2021-07-27 Arup Laboratories, Inc. Separating and quantifying unbound target analytes from biological samples
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
WO2019173731A1 (en) * 2018-03-08 2019-09-12 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
EP3810650A1 (en) * 2018-06-19 2021-04-28 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
EP4509831A3 (en) * 2018-07-27 2025-03-19 Waters Technologies Corporation Liquid chromatography/mass spectrometry methods for the analysis of polar molecules
WO2020036869A1 (en) 2018-08-16 2020-02-20 Emd Millipore Corporation Closed bioprocessing device
CN111215027A (zh) * 2018-11-26 2020-06-02 中国科学院大连化学物理研究所 一种水热碳微球色谱介质的修饰方法
WO2020117760A1 (en) * 2018-12-03 2020-06-11 Duke University Purification method for recombinant proteins and nanoparticles
KR102120675B1 (ko) * 2018-12-05 2020-06-09 유바이오주식회사 바이러스 클리어런스 공정을 포함하는 봉독의 정제방법 및 이를 이용한 염증성 질환 예방 또는 치료용 조성물
CN113195519B (zh) 2018-12-12 2024-08-30 唐纳森公司 亲和膜及其制备方法
SG11202107192VA (en) * 2019-01-23 2021-07-29 Daiichi Sankyo Co Ltd Methods for purifying antibodies comprising of a process by using activated carbon materials
KR20210148288A (ko) * 2019-04-02 2021-12-07 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치 생체내 리폴딩된 항체 단편의 클로닝 및 발현
EP3962924A1 (en) 2019-05-03 2022-03-09 Genentech, Inc. Methods of reducing the enzymatic hydrolysis activity rate in a composition obtained from a purification platform
US11896969B2 (en) 2019-09-05 2024-02-13 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resins
EP3931303A1 (en) 2019-12-06 2022-01-05 Regeneron Pharmaceuticals, Inc. Anti-vegf protein compositions and methods for producing the same
US20210178334A1 (en) * 2019-12-16 2021-06-17 Dionex Corporation Electrolytic eluent generators with stabilized operating voltages
CN115397834A (zh) * 2020-04-13 2022-11-25 3M创新有限公司 用于纯化目标分子的流通方法和装置
WO2021226444A2 (en) 2020-05-08 2021-11-11 Regeneron Pharmaceuticals, Inc. Vegf traps and mini-traps and methods for treating ocular disorders and cancer
EP4237116A1 (en) * 2020-10-30 2023-09-06 Genentech, Inc. Purification platforms for obtaining pharmaceutical compositions having a reduced hydrolytic enzyme activity rate
CN116829569A (zh) * 2020-11-20 2023-09-29 阿雷斯贸易股份有限公司 蛋白质纯化缓冲液和方法
CA3203540A1 (en) * 2020-12-28 2022-07-07 Eugene ZURLO Systems and methods for process scale isolation of immunoglobulin g
CN112870979B (zh) * 2020-12-31 2022-12-23 内蒙古金达威药业有限公司 一种β-烟酰胺单核苷酸的分离纯化方法
CN115326937B (zh) * 2021-05-11 2024-06-18 山东省食品药品检验研究院 一种基因毒杂质捕捉用固相探针及其使用方法与应用
CA3236213A1 (en) 2021-10-27 2023-05-04 Eugene ZURLO Compositions and methods for isolating proteins
CN116120392B (zh) * 2023-04-18 2023-08-01 上海健士拜生物科技有限公司 聚体蛋白的纯化方法
CN118126110B (zh) * 2024-05-10 2024-08-06 江苏赛孚士生物技术有限公司 一种纯化单克隆抗体的方法

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US31093A (en) 1861-01-08 Mode of constructing iron pavements
USRE31093E (en) 1978-05-10 1982-11-30 Westvaco Corporation Process for making activated carbon with control of metal ion concentration in phosphoric acid
JPS5918731A (ja) 1982-07-21 1984-01-31 Toray Ind Inc イオン交換繊維
US4639513A (en) * 1984-02-02 1987-01-27 Cuno Inc. Intravenously injectable immunoglobulin G (IGG) and method for producing same
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DE3927112C1 (enExample) * 1989-08-17 1990-10-25 Biotest Pharma Gmbh, 6072 Dreieich, De
GB8923662D0 (en) 1989-10-20 1989-12-06 Norit Uk Ltd A method of producing granular activated carbon
JPH0778025B2 (ja) * 1990-03-20 1995-08-23 日本赤十字社 免疫グロブリンｇの製造方法
US5204310A (en) 1992-02-21 1993-04-20 Westvaco Corporation High activity, high density activated carbon
US5612558A (en) 1995-11-15 1997-03-18 Micron Technology, Inc. Hemispherical grained silicon on refractory metal nitride
US20020056686A1 (en) * 1996-06-14 2002-05-16 Agathagelos Kyrlidis Chromatography and other adsorptions using modified carbon adsorbents
DE29801192U1 (de) 1998-01-26 1998-08-20 Hans Bernstein Spezialfabrik für Schaltkontakte GmbH & Co, 32457 Porta Westfalica Radiusbetätiger für Sicherheitsschalter
DE19935160A1 (de) 1999-07-27 2001-02-15 Grove Us Llc Shady Grove Vorrichtung zur Erhöhung der Lenkfähigkeit getriebener Achsen
US6569910B1 (en) 1999-10-27 2003-05-27 Basf Aktiengesellschaft Ion exchange resins and methods of making the same
US20040028901A1 (en) 2002-02-25 2004-02-12 Rumpf Frederick H. Compositions comprising continuous networks and monoliths
KR101035043B1 (ko) 2002-07-01 2011-05-19 아르키온 라이프 사이언씨즈 엘엘씨 글루코사민 및 ｎ-아세틸글루코사민의 제조를 위한 물질및 공정
GB0304576D0 (en) 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
EP1601697B1 (en) 2003-02-28 2007-05-30 Lonza Biologics plc Antibody purification by Protein A and ion exchange chromatography
US20080058507A1 (en) 2004-02-11 2008-03-06 Hui Liu Method For The Removal Of Aggregate Proteins From Recombinant Samples Using Ion Exchange Chromatography
SE0402558D0 (sv) * 2004-10-21 2004-10-21 Amersham Biosciences Ab A process for the purification of antibodies
US7390403B2 (en) 2004-03-19 2008-06-24 Millipore Corporation Prefilter system for biological systems
MX2007008132A (es) * 2004-12-30 2007-07-20 Schering Plough Ltd Vacuna para aumentar el crecimiento basada en epitopes neutralizantes.
WO2007063129A2 (en) 2005-12-02 2007-06-07 Novozymes A/S Insolation of peptides , polypeptides and proteins
WO2007067689A2 (en) 2005-12-08 2007-06-14 Waters Investments Limited Device and methods for preparation of peptides and proteins samples from solution
AU2007291282B2 (en) * 2006-08-28 2011-10-20 Ares Trading S.A. Process for the purification of Fc-fusion proteins
CA2570956C (en) * 2006-12-12 2014-04-01 Norgen Biotek Corporation Method of using silicon carbide for removal of adventitious agents
RU2009148891A (ru) 2007-06-01 2011-07-20 Ф.Хоффманн-Ля Рош Аг (Ch) Очистка иммуноглобулина
JP2010279461A (ja) 2009-06-03 2010-12-16 Daicen Membrane Systems Ltd 人工透析液又は人工透析用水の清浄化方法
US8945895B2 (en) 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
SG178276A1 (en) 2009-08-06 2012-03-29 Genentech Inc Method to improve virus removal in protein purification
CN102574911B (zh) 2009-08-07 2017-06-06 Emd密理博公司 从样品的一或多种杂质中纯化靶蛋白的方法
US9527010B2 (en) * 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
EP2519537A4 (en) 2009-12-29 2013-07-10 Reddys Lab Ltd Dr CLEANING PROTEINS
EP2744518A2 (en) * 2011-08-19 2014-06-25 EMD Millipore Corporation Use of small molecules in methods for purification of biomolecules
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
SG11201508835YA (en) * 2013-07-12 2015-11-27 Merck Patent Gmbh Removal of fragments from a sample containing a target protein using activated carbon

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