JP2015071629A5 - - Google Patents
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- JP2015071629A5 JP2015071629A5 JP2014246058A JP2014246058A JP2015071629A5 JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5 JP 2014246058 A JP2014246058 A JP 2014246058A JP 2014246058 A JP2014246058 A JP 2014246058A JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5
- Authority
- JP
- Japan
- Prior art keywords
- eluate
- sample
- flow
- protein
- hcp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 20
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 14
- 102000004169 proteins and genes Human genes 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 11
- 239000002609 medium Substances 0.000 claims 7
- 238000005349 anion exchange Methods 0.000 claims 4
- 239000003575 carbonaceous material Substances 0.000 claims 4
- 239000002775 capsule Substances 0.000 claims 2
- 238000004587 chromatography analysis Methods 0.000 claims 2
- 239000012501 chromatography medium Substances 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 238000011100 viral filtration Methods 0.000 claims 2
- 239000006096 absorbing agent Substances 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 230000003068 static effect Effects 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161575349P | 2011-08-19 | 2011-08-19 | |
| US61/575,349 | 2011-08-19 | ||
| US201261666240P | 2012-06-29 | 2012-06-29 | |
| US61/666,240 | 2012-06-29 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Division JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015071629A JP2015071629A (ja) | 2015-04-16 |
| JP2015071629A5 true JP2015071629A5 (enExample) | 2016-03-31 |
| JP6254072B2 JP6254072B2 (ja) | 2017-12-27 |
Family
ID=47076067
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Active JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2014246058A Active JP6254072B2 (ja) | 2011-08-19 | 2014-12-04 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2016022509A Active JP6339120B2 (ja) | 2011-08-19 | 2016-02-09 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2018017028A Active JP6743074B2 (ja) | 2011-08-19 | 2018-02-02 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Active JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016022509A Active JP6339120B2 (ja) | 2011-08-19 | 2016-02-09 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2018017028A Active JP6743074B2 (ja) | 2011-08-19 | 2018-02-02 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US9096648B2 (enExample) |
| EP (2) | EP2942353B1 (enExample) |
| JP (4) | JP5938295B2 (enExample) |
| KR (1) | KR101484659B1 (enExample) |
| CN (3) | CN102977182B (enExample) |
| ES (2) | ES2539232T3 (enExample) |
| TW (1) | TWI574975B (enExample) |
| WO (1) | WO2013028330A2 (enExample) |
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| US9096648B2 (en) | 2011-08-19 | 2015-08-04 | Emd Millipore Corporation | Methods of reducing level of one or more impurities in a sample during protein purification |
| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| JP6182210B2 (ja) * | 2012-06-29 | 2017-08-16 | イー・エム・デイー・ミリポア・コーポレイシヨン | タンパク質精製プロセス中のウイルスの不活性化方法 |
| EP2682168A1 (en) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| US9650411B2 (en) * | 2012-08-07 | 2017-05-16 | Kyowa Hakko Kirin Co., Ltd. | Method of purifying protein |
| CA2893760C (en) * | 2013-02-26 | 2018-07-24 | Emd Millipore Corporation | Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions |
| DK2968494T3 (en) | 2013-03-13 | 2019-03-04 | Seattle Genetics Inc | ACTIVATED CARBON FILTERING FOR CLEANING BENZODIAZEPINE ADCs |
| DK2994485T3 (en) | 2013-05-06 | 2018-04-23 | Sanofi Sa | CONTINUOUS MULTIPLE STEP PROCEDURE FOR PURIFICATION OF ANTIBODIES |
| US10519194B2 (en) | 2013-07-12 | 2019-12-31 | Merck Patent Gmbh | Removal of fragments from a sample containing a target protein using activated carbon |
| CA2912765C (en) | 2013-07-12 | 2018-05-01 | Emd Millipore Corporation | A method of determining virus removal from a sample containing a target protein using activated carbon |
| SG11201601823TA (en) * | 2013-09-13 | 2016-04-28 | Genentech Inc | Methods and compositions comprising purified recombinant polypeptides |
| CN103497248B (zh) * | 2013-09-22 | 2016-08-10 | 中国抗体制药有限公司 | 一种从细胞培养上清中分离纯化抗体的方法 |
| US9546208B2 (en) | 2014-01-03 | 2017-01-17 | Bio-Rad Laboratories, Inc. | Removal of impurities from protein A eluates |
| CA2942134C (en) * | 2014-03-04 | 2023-08-15 | Merck Patent Gmbh | Robust antibody purification |
| WO2015137860A1 (en) * | 2014-03-14 | 2015-09-17 | Ge Healthcare Bio-Sciences Ab | Separation matrices for purification of biological particles |
| CA2966764C (en) * | 2014-11-06 | 2022-10-18 | Merck Patent Gmbh | Activated carbon for the removal of leachables and/or extractables |
| CN107922476A (zh) * | 2015-03-13 | 2018-04-17 | 诺夫免疫股份有限公司 | 纯化双特异性抗体的方法 |
| ES2964640T3 (es) * | 2015-08-13 | 2024-04-08 | Amgen Inc | Filtración en profundidad cargada de proteínas de unión al antígeno |
| CN108473558A (zh) * | 2015-08-20 | 2018-08-31 | 豪夫迈·罗氏有限公司 | Fkpa的纯化及其用于生产重组多肽的用途 |
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| KR20180125999A (ko) * | 2016-03-17 | 2018-11-26 | 메르크 파텐트 게엠베하 | 폴록사머의 정제 방법 |
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| US20200247911A1 (en) * | 2017-05-17 | 2020-08-06 | Msd Wellcome Trust Hilleman Laboratories Pvt. Ltd. | Purification of Bacterial Polysaccharides |
| CN107236038B (zh) * | 2017-06-20 | 2020-11-27 | 华兰生物工程股份有限公司 | 微晶纤维素提高蛋白质澄清度的方法及其产品和应用 |
| MA49962A (fr) | 2017-08-22 | 2020-07-01 | Biogen Ma Inc | Procédés de purification d'anticorps présentant des agrégats réduits à poids moléculaire élevé |
| AU2018404665A1 (en) | 2017-08-31 | 2020-02-27 | Massachusetts Institute Of Technology | Hollow fibre membrane filtration systems and methods for manufacturing biologically-produced products |
| US11071927B2 (en) * | 2017-11-15 | 2021-07-27 | Arup Laboratories, Inc. | Separating and quantifying unbound target analytes from biological samples |
| CN109929038B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | Vegf捕获剂融合蛋白的纯化方法 |
| CN111818980B (zh) * | 2018-03-08 | 2022-10-11 | 生物辐射实验室股份有限公司 | 阴离子交换-疏水混合模式色谱树脂 |
| EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| WO2019246153A1 (en) * | 2018-06-19 | 2019-12-26 | Bristol-Myers Squibb Company | Methods of purifying proteins using chromatography |
| US11391707B2 (en) * | 2018-07-27 | 2022-07-19 | Waters Technologies Corporation | Liquid chromatography/mass spectrometry methods for the analysis of polar molecules |
| CA3107770C (en) | 2018-08-16 | 2023-09-05 | Emd Millipore Corporation | Closed bioprocessing device |
| CN111215027A (zh) * | 2018-11-26 | 2020-06-02 | 中国科学院大连化学物理研究所 | 一种水热碳微球色谱介质的修饰方法 |
| WO2020117760A1 (en) * | 2018-12-03 | 2020-06-11 | Duke University | Purification method for recombinant proteins and nanoparticles |
| KR102120675B1 (ko) * | 2018-12-05 | 2020-06-09 | 유바이오주식회사 | 바이러스 클리어런스 공정을 포함하는 봉독의 정제방법 및 이를 이용한 염증성 질환 예방 또는 치료용 조성물 |
| CA3118156A1 (en) | 2018-12-12 | 2020-06-18 | Purilogics, LLC | Affinity membrane and method of preparation |
| WO2020153390A1 (ja) | 2019-01-23 | 2020-07-30 | 第一三共株式会社 | 活性炭材料を使用する工程を含む抗体精製方法 |
| WO2020202208A1 (en) * | 2019-04-02 | 2020-10-08 | Council Of Scientific & Industrial Research | Cloning and expression of in-vivo refolded antibody fragment |
| AU2020268896B2 (en) | 2019-05-03 | 2024-11-28 | F. Hoffmann-La Roche Ag | Methods of reducing the enzymatic hydrolysis activity rate in a composition obtained from a purification platform |
| CN114040815B (zh) | 2019-09-05 | 2024-06-28 | 生物辐射实验室股份有限公司 | 阴离子交换-疏水混合模式色谱树脂 |
| IL309560B2 (en) | 2019-12-06 | 2025-05-01 | Regeneron Pharma | Anti-VEGF protein preparations and methods for their production |
| US20210178334A1 (en) * | 2019-12-16 | 2021-06-17 | Dionex Corporation | Electrolytic eluent generators with stabilized operating voltages |
| AU2021208515A1 (en) | 2020-01-15 | 2022-08-04 | F. Hoffmann-La Roche Ag | Methods to decrease impurities from recombinant protein manufacturing processes |
| EP4136093A4 (en) * | 2020-04-13 | 2025-08-06 | Solventum Intellectual Properties Company | CONTINUOUS FLOW METHODS AND DEVICES FOR PURIFYING A TARGET MOLECULE |
| MX2022013812A (es) | 2020-05-08 | 2022-12-15 | Regeneron Pharma | Trampas y mini-trampas de vegf y metodos para el tratamiento de trastornos oculares y cancer. |
| IL302333A (en) * | 2020-10-30 | 2023-06-01 | Genentech Inc | Purification platforms for obtaining pharmaceutical preparations with a reduced hydrolytic enzyme activity rate |
| IL302981A (en) * | 2020-11-20 | 2023-07-01 | Ares Trading Sa | Methods for purifying proteins |
| JP2024501025A (ja) * | 2020-12-28 | 2024-01-10 | プラズマ テクノロジーズ エルエルシー | 免疫グロブリンgのプロセススケールでの単離のためのシステム及び方法 |
| CN112870979B (zh) * | 2020-12-31 | 2022-12-23 | 内蒙古金达威药业有限公司 | 一种β-烟酰胺单核苷酸的分离纯化方法 |
| CN115326937B (zh) * | 2021-05-11 | 2024-06-18 | 山东省食品药品检验研究院 | 一种基因毒杂质捕捉用固相探针及其使用方法与应用 |
| CA3236213A1 (en) | 2021-10-27 | 2023-05-04 | Eugene ZURLO | Compositions and methods for isolating proteins |
| CN116375808B (zh) * | 2022-12-30 | 2026-07-17 | 中国科学院武汉病毒研究所 | 一种huh固相结合蛋白及其介导的蛋白或核酸的固相结合方法 |
| CN116120392B (zh) * | 2023-04-18 | 2023-08-01 | 上海健士拜生物科技有限公司 | 聚体蛋白的纯化方法 |
| CN118126110B (zh) * | 2024-05-10 | 2024-08-06 | 江苏赛孚士生物技术有限公司 | 一种纯化单克隆抗体的方法 |
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| US31093A (en) | 1861-01-08 | Mode of constructing iron pavements | ||
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| US4639513A (en) | 1984-02-02 | 1987-01-27 | Cuno Inc. | Intravenously injectable immunoglobulin G (IGG) and method for producing same |
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| SG10201701224UA (en) * | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| EP2682168A1 (en) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| US10519194B2 (en) * | 2013-07-12 | 2019-12-31 | Merck Patent Gmbh | Removal of fragments from a sample containing a target protein using activated carbon |
-
2012
- 2012-08-02 US US13/565,463 patent/US9096648B2/en active Active
- 2012-08-02 WO PCT/US2012/049351 patent/WO2013028330A2/en not_active Ceased
- 2012-08-07 TW TW101128407A patent/TWI574975B/zh active
- 2012-08-09 EP EP15159525.3A patent/EP2942353B1/en active Active
- 2012-08-09 ES ES12179861.5T patent/ES2539232T3/es active Active
- 2012-08-09 ES ES15159525.3T patent/ES2677650T3/es active Active
- 2012-08-09 EP EP12179861.5A patent/EP2574618B1/en active Active
- 2012-08-13 JP JP2012179197A patent/JP5938295B2/ja active Active
- 2012-08-16 KR KR20120089449A patent/KR101484659B1/ko active Active
- 2012-08-20 CN CN201210398359.XA patent/CN102977182B/zh active Active
- 2012-08-20 CN CN202010257856.2A patent/CN111423488B/zh active Active
- 2012-08-20 CN CN201610090272.4A patent/CN105669829B/zh active Active
-
2014
- 2014-12-04 JP JP2014246058A patent/JP6254072B2/ja active Active
-
2015
- 2015-06-23 US US14/747,029 patent/US10287314B2/en active Active
-
2016
- 2016-02-09 JP JP2016022509A patent/JP6339120B2/ja active Active
-
2018
- 2018-02-02 JP JP2018017028A patent/JP6743074B2/ja active Active
-
2019
- 2019-03-20 US US16/358,845 patent/US11634457B2/en active Active
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