CN111423488B - 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法 - Google Patents

在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法 Download PDF

Info

Publication number
CN111423488B
CN111423488B CN202010257856.2A CN202010257856A CN111423488B CN 111423488 B CN111423488 B CN 111423488B CN 202010257856 A CN202010257856 A CN 202010257856A CN 111423488 B CN111423488 B CN 111423488B
Authority
CN
China
Prior art keywords
protein
eluate
activated carbon
column
impurities
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010257856.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN111423488A (zh
Inventor
N·比安
C·吉莱斯皮
M·T·斯通
M·科兹洛夫
J·陈
M·瓦克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
EMD Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EMD Millipore Corp filed Critical EMD Millipore Corp
Priority to CN202010257856.2A priority Critical patent/CN111423488B/zh
Publication of CN111423488A publication Critical patent/CN111423488A/zh
Application granted granted Critical
Publication of CN111423488B publication Critical patent/CN111423488B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/36Extraction; Separation; Purification by a combination of two or more processes of different types
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/10Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/12Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
    • B01D15/125Pre-filtration
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/363Anion-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/20Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising free carbon; comprising carbon obtained by carbonising processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282Porous sorbents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26Cation exchangers for chromatographic processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20Anion exchangers for chromatographic processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J43/00Amphoteric ion-exchange, i.e. using ion-exchangers having cationic and anionic groups; Use of material as amphoteric ion-exchangers; Treatment of material for improving their amphoteric ion-exchange properties
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00Ion-exchange processes in general; Apparatus therefor
    • B01J47/02Column or bed processes
    • B01J47/04Mixed-bed processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/20Partition-, reverse-phase or hydrophobic interaction chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/10Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804Affinity chromatography
    • B01D15/3809Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN202010257856.2A 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法 Active CN111423488B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010257856.2A CN111423488B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201161575349P 2011-08-19 2011-08-19
US61/575,349 2011-08-19
US201261666240P 2012-06-29 2012-06-29
US61/666,240 2012-06-29
CN202010257856.2A CN111423488B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法
CN201210398359.XA CN102977182B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质水平的方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201210398359.XA Division CN102977182B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质水平的方法

Publications (2)

Publication Number Publication Date
CN111423488A CN111423488A (zh) 2020-07-17
CN111423488B true CN111423488B (zh) 2024-08-16

Family

ID=47076067

Family Applications (3)

Application Number Title Priority Date Filing Date
CN202010257856.2A Active CN111423488B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法
CN201610090272.4A Active CN105669829B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法
CN201210398359.XA Active CN102977182B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质水平的方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN201610090272.4A Active CN105669829B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法
CN201210398359.XA Active CN102977182B (zh) 2011-08-19 2012-08-20 在蛋白质纯化过程中降低样品中的一种或多种杂质水平的方法

Country Status (8)

Country Link
US (3) US9096648B2 (enExample)
EP (2) EP2574618B1 (enExample)
JP (4) JP5938295B2 (enExample)
KR (1) KR101484659B1 (enExample)
CN (3) CN111423488B (enExample)
ES (2) ES2677650T3 (enExample)
TW (1) TWI574975B (enExample)
WO (1) WO2013028330A2 (enExample)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10507409B2 (en) * 2016-03-12 2019-12-17 Spf Technologies, Llc Hyper-productive chromatography system and process
US11395980B2 (en) 2010-01-25 2022-07-26 Spf Technologies Llc Chromatographic cassette
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
SG11201406407TA (en) * 2012-06-29 2014-11-27 Emd Millipore Corp Methods for inactivating viruses during a protein purification process
US9650411B2 (en) * 2012-08-07 2017-05-16 Kyowa Hakko Kirin Co., Ltd. Method of purifying protein
WO2014133741A1 (en) * 2013-02-26 2014-09-04 Emd Millipore Corporation Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions
WO2014143622A1 (en) * 2013-03-13 2014-09-18 Seattle Genetics, Inc. ACTIVATED CARBON FILTRATION FOR PURIFICATION OF BENZODIAZEPINE ADCs
TWI631132B (zh) * 2013-05-06 2018-08-01 賽諾菲公司 用於純化抗體之連續多步驟方法
JP6141524B2 (ja) * 2013-07-12 2017-06-07 メルク パテント ゲーエムベーハー 活性炭を用いた標的タンパク質含有サンプルからのフラグメントの除去
JP2016529885A (ja) 2013-07-12 2016-09-29 イー・エム・デイー・ミリポア・コーポレイシヨン 活性炭を使用して標的タンパク質を含む試料からのウイルス除去を決定する方法
KR102571391B1 (ko) * 2013-09-13 2023-08-29 제넨테크, 인크. 정제된 재조합 폴리펩티드를 포함하는 방법 및 조성물
CN103497248B (zh) * 2013-09-22 2016-08-10 中国抗体制药有限公司 一种从细胞培养上清中分离纯化抗体的方法
US9546208B2 (en) 2014-01-03 2017-01-17 Bio-Rad Laboratories, Inc. Removal of impurities from protein A eluates
SG10201807572PA (en) * 2014-03-04 2018-10-30 Merck Patent Gmbh Robust antibody purification
JP6702587B2 (ja) * 2014-03-14 2020-06-03 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ 生物粒子の精製用分離マトリックス
CA2966764C (en) 2014-11-06 2022-10-18 Merck Patent Gmbh Activated carbon for the removal of leachables and/or extractables
JP6894843B2 (ja) * 2015-03-13 2021-06-30 ノビミューン エスアー 二重特異性抗体を精製する方法
DK3334747T5 (da) 2015-08-13 2024-10-07 Amgen Inc Ladet dybdefiltrering af antigenbindende proteiner
WO2017031476A2 (en) * 2015-08-20 2017-02-23 Genentech, Inc. Purification of fkpa and uses thereof for producing recombinant polypeptides
US12409440B1 (en) 2016-01-26 2025-09-09 Spf Technologies Llc Separation module
CN108779242B (zh) * 2016-03-17 2021-06-11 默克专利股份公司 纯化泊洛沙姆的方法
US20200283472A1 (en) * 2016-03-29 2020-09-10 Navya Biologicals Pvt. Ltd A process for purification of fc-fusion proteins
KR102413808B1 (ko) * 2016-04-01 2022-06-29 유씨비 바이오파마 에스알엘 단백질 정제 방법
CN109964123A (zh) * 2016-11-18 2019-07-02 捷恩智株式会社 抗体的纯化方法
US20200224144A1 (en) * 2017-04-01 2020-07-16 Massachusetts Institute Of Technology Systems and methods for manufacturing biologically-produced products
CN110892077A (zh) * 2017-05-17 2020-03-17 默沙东和惠康基金会合资的希勒曼实验室私人有限公司 细菌多糖的纯化
CN107236038B (zh) * 2017-06-20 2020-11-27 华兰生物工程股份有限公司 微晶纤维素提高蛋白质澄清度的方法及其产品和应用
US11697670B2 (en) 2017-08-22 2023-07-11 Biogen Ma Inc. Methods for purifying antibodies having reduced high molecular weight aggregates
KR20200042532A (ko) 2017-08-31 2020-04-23 메사추세츠 인스티튜트 오브 테크놀로지 생물학적으로 생성된 생성물을 제조하기 위한 중공 섬유 멤브레인 여과 시스템 및 방법
US11071927B2 (en) * 2017-11-15 2021-07-27 Arup Laboratories, Inc. Separating and quantifying unbound target analytes from biological samples
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
CN111818980B (zh) * 2018-03-08 2022-10-11 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
CN112566930A (zh) * 2018-06-19 2021-03-26 百时美施贵宝公司 使用色谱纯化蛋白质的方法
EP4509831A3 (en) * 2018-07-27 2025-03-19 Waters Technologies Corporation Liquid chromatography/mass spectrometry methods for the analysis of polar molecules
JP7228675B2 (ja) 2018-08-16 2023-02-24 イー・エム・デイー・ミリポア・コーポレイシヨン 閉鎖バイオプロセシングデバイス
CN111215027A (zh) * 2018-11-26 2020-06-02 中国科学院大连化学物理研究所 一种水热碳微球色谱介质的修饰方法
WO2020117760A1 (en) * 2018-12-03 2020-06-11 Duke University Purification method for recombinant proteins and nanoparticles
KR102120675B1 (ko) * 2018-12-05 2020-06-09 유바이오주식회사 바이러스 클리어런스 공정을 포함하는 봉독의 정제방법 및 이를 이용한 염증성 질환 예방 또는 치료용 조성물
JP7565276B2 (ja) 2018-12-12 2024-10-10 ドナルドソン カンパニー,インコーポレイティド 親和性膜及び製造方法
CA3127174A1 (en) 2019-01-23 2020-07-30 Daiichi Sankyo Company, Limited Methods for purifying antibodies comprising of a process by using activated carbon materials
KR20210148288A (ko) * 2019-04-02 2021-12-07 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치 생체내 리폴딩된 항체 단편의 클로닝 및 발현
AU2020268896B2 (en) 2019-05-03 2024-11-28 F. Hoffmann-La Roche Ag Methods of reducing the enzymatic hydrolysis activity rate in a composition obtained from a purification platform
CN114040815B (zh) 2019-09-05 2024-06-28 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
US11186625B2 (en) 2019-12-06 2021-11-30 Regeneran Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
US20210178334A1 (en) * 2019-12-16 2021-06-17 Dionex Corporation Electrolytic eluent generators with stabilized operating voltages
JP2023521193A (ja) * 2020-04-13 2023-05-23 スリーエム イノベイティブ プロパティズ カンパニー フロースループロセス及び標的分子を精製するためのデバイス
AU2021268026A1 (en) 2020-05-08 2023-01-19 Regeneron Pharmaceuticals, Inc. VEGF traps and mini-traps and methods for treating ocular disorders and cancer
CN116507395A (zh) * 2020-10-30 2023-07-28 基因泰克公司 用于获得具有降低的水解酶活性率的药物组合物的纯化平台
CN116829569A (zh) * 2020-11-20 2023-09-29 阿雷斯贸易股份有限公司 蛋白质纯化缓冲液和方法
EP4271706A4 (en) 2020-12-28 2025-01-15 Plasma Technologies LLC SYSTEMS AND METHODS FOR THE ISOLATION OF IMMUNGLOBULIN G ON A PROCESS SCALE
CN112870979B (zh) * 2020-12-31 2022-12-23 内蒙古金达威药业有限公司 一种β-烟酰胺单核苷酸的分离纯化方法
CN115326937B (zh) * 2021-05-11 2024-06-18 山东省食品药品检验研究院 一种基因毒杂质捕捉用固相探针及其使用方法与应用
US12037360B2 (en) 2021-10-27 2024-07-16 Plasma Technologies, Llc Compositions and methods for isolating proteins
CN116120392B (zh) * 2023-04-18 2023-08-01 上海健士拜生物科技有限公司 聚体蛋白的纯化方法
CN118126110B (zh) * 2024-05-10 2024-08-06 江苏赛孚士生物技术有限公司 一种纯化单克隆抗体的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1577319A1 (en) * 2004-03-19 2005-09-21 Millipore Corporation Prefilter system for biological systems
WO2008025747A1 (en) * 2006-08-28 2008-03-06 Ares Trading S.A. Process for the purification of fc-fusion proteins

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US31093A (en) 1861-01-08 Mode of constructing iron pavements
USRE31093E (en) 1978-05-10 1982-11-30 Westvaco Corporation Process for making activated carbon with control of metal ion concentration in phosphoric acid
JPS5918731A (ja) 1982-07-21 1984-01-31 Toray Ind Inc イオン交換繊維
US4639513A (en) * 1984-02-02 1987-01-27 Cuno Inc. Intravenously injectable immunoglobulin G (IGG) and method for producing same
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DE3927112C1 (enExample) * 1989-08-17 1990-10-25 Biotest Pharma Gmbh, 6072 Dreieich, De
GB8923662D0 (en) 1989-10-20 1989-12-06 Norit Uk Ltd A method of producing granular activated carbon
JPH0778025B2 (ja) * 1990-03-20 1995-08-23 日本赤十字社 免疫グロブリンgの製造方法
US5204310A (en) 1992-02-21 1993-04-20 Westvaco Corporation High activity, high density activated carbon
US5612558A (en) 1995-11-15 1997-03-18 Micron Technology, Inc. Hemispherical grained silicon on refractory metal nitride
US20020056686A1 (en) * 1996-06-14 2002-05-16 Agathagelos Kyrlidis Chromatography and other adsorptions using modified carbon adsorbents
DE29801192U1 (de) 1998-01-26 1998-08-20 Hans Bernstein Spezialfabrik für Schaltkontakte GmbH & Co, 32457 Porta Westfalica Radiusbetätiger für Sicherheitsschalter
DE19935160A1 (de) 1999-07-27 2001-02-15 Grove Us Llc Shady Grove Vorrichtung zur Erhöhung der Lenkfähigkeit getriebener Achsen
US6569910B1 (en) 1999-10-27 2003-05-27 Basf Aktiengesellschaft Ion exchange resins and methods of making the same
US20040028901A1 (en) 2002-02-25 2004-02-12 Rumpf Frederick H. Compositions comprising continuous networks and monoliths
MXPA04012978A (es) 2002-07-01 2005-09-12 Arkion Life Sciences Llc D B A Proceso y material para la produccion de glucosamina y n-acetilglucosamina.
DK1601697T3 (da) 2003-02-28 2007-10-01 Lonza Biologics Plc Oprensning af antistof ved protein A- og ionbytningskromatografi
GB0304576D0 (en) 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
WO2005077130A2 (en) 2004-02-11 2005-08-25 Tanox, Inc. A method for the removal of aggregate proteins from recombinant samples using ion exchange chromatography
SE0402558D0 (sv) * 2004-10-21 2004-10-21 Amersham Biosciences Ab A process for the purification of antibodies
AU2005323087A1 (en) * 2004-12-30 2006-07-13 Schering-Plough Pty. Limited Neutralizing epitope-based growth enhancing vaccine
WO2007063129A2 (en) 2005-12-02 2007-06-07 Novozymes A/S Insolation of peptides , polypeptides and proteins
WO2007067689A2 (en) 2005-12-08 2007-06-14 Waters Investments Limited Device and methods for preparation of peptides and proteins samples from solution
CA2570956C (en) * 2006-12-12 2014-04-01 Norgen Biotek Corporation Method of using silicon carbide for removal of adventitious agents
AU2008256411B2 (en) * 2007-06-01 2013-08-22 F. Hoffmann-La Roche Ag Immunoglobulin purification
JP2010279461A (ja) 2009-06-03 2010-12-16 Daicen Membrane Systems Ltd 人工透析液又は人工透析用水の清浄化方法
US8945895B2 (en) 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
PT2462158T (pt) 2009-08-06 2018-02-23 H Hoffnabb La Roche Ag Método para melhorar a remoção de vírus na purificação de proteínas
WO2011017514A1 (en) * 2009-08-07 2011-02-10 Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
US9527010B2 (en) * 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
EP2519537A4 (en) * 2009-12-29 2013-07-10 Reddys Lab Ltd Dr CLEANING PROTEINS
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
JP2014527528A (ja) * 2011-08-19 2014-10-16 イー・エム・デイー・ミリポア・コーポレイシヨン 生体分子の精製方法のための小分子の使用
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
JP6141524B2 (ja) * 2013-07-12 2017-06-07 メルク パテント ゲーエムベーハー 活性炭を用いた標的タンパク質含有サンプルからのフラグメントの除去

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1577319A1 (en) * 2004-03-19 2005-09-21 Millipore Corporation Prefilter system for biological systems
WO2008025747A1 (en) * 2006-08-28 2008-03-06 Ares Trading S.A. Process for the purification of fc-fusion proteins

Also Published As

Publication number Publication date
EP2942353A1 (en) 2015-11-11
US9096648B2 (en) 2015-08-04
WO2013028330A2 (en) 2013-02-28
US20130197200A1 (en) 2013-08-01
JP6339120B2 (ja) 2018-06-06
JP6743074B2 (ja) 2020-08-19
KR101484659B1 (ko) 2015-01-21
ES2677650T3 (es) 2018-08-06
CN111423488A (zh) 2020-07-17
US20190218250A1 (en) 2019-07-18
JP6254072B2 (ja) 2017-12-27
JP5938295B2 (ja) 2016-06-22
CN105669829A (zh) 2016-06-15
JP2015071629A (ja) 2015-04-16
CN105669829B (zh) 2020-04-21
EP2574618A1 (en) 2013-04-03
ES2539232T3 (es) 2015-06-29
US20160016992A1 (en) 2016-01-21
CN102977182B (zh) 2016-03-02
TWI574975B (zh) 2017-03-21
JP2016085231A (ja) 2016-05-19
KR20130020950A (ko) 2013-03-04
CN102977182A (zh) 2013-03-20
EP2574618B1 (en) 2015-03-18
US11634457B2 (en) 2023-04-25
EP2942353B1 (en) 2018-05-09
WO2013028330A3 (en) 2013-08-22
TW201313735A (zh) 2013-04-01
US10287314B2 (en) 2019-05-14
JP2018091858A (ja) 2018-06-14
JP2013044748A (ja) 2013-03-04

Similar Documents

Publication Publication Date Title
CN111423488B (zh) 在蛋白质纯化过程中降低样品中的一种或多种杂质的水平的方法
KR101415660B1 (ko) 샘플 중의 1 이상의 불순물로부터 표적 단백질을 정제하는 방법
KR101921552B1 (ko) 사전 세정 단계를 이용하는 면역글로불린 정제
AU2004286938B2 (en) Removal of high molecular weight aggregates using hydroxyapatite chromatography
KR102070566B1 (ko) 크로마토그래피 방법
JP6484169B2 (ja) 生体分子の精製
CN113710685B (zh) 通过使用捕获前的絮凝改善免疫球蛋白的亲和色谱
RU2794431C1 (ru) Улучшение аффинной хроматографии иммуноглобулинов путем применения флокуляции до захвата
CN118291432A (zh) 一种利用非亲和层析捕获技术纯化蛋白的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant