JP2014517273A - 生物学的試料の固定および保存のための固定液 - Google Patents
生物学的試料の固定および保存のための固定液 Download PDFInfo
- Publication number
- JP2014517273A JP2014517273A JP2014508879A JP2014508879A JP2014517273A JP 2014517273 A JP2014517273 A JP 2014517273A JP 2014508879 A JP2014508879 A JP 2014508879A JP 2014508879 A JP2014508879 A JP 2014508879A JP 2014517273 A JP2014517273 A JP 2014517273A
- Authority
- JP
- Japan
- Prior art keywords
- fixative
- dibromo
- fixing
- solvent
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000834 fixative Substances 0.000 title claims abstract description 26
- 239000012472 biological sample Substances 0.000 title claims abstract description 5
- 238000003860 storage Methods 0.000 title claims description 7
- 239000012620 biological material Substances 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 26
- 239000000126 substance Substances 0.000 claims description 19
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- UUIVKBHZENILKB-UHFFFAOYSA-N 2,2-dibromo-2-cyanoacetamide Chemical compound NC(=O)C(Br)(Br)C#N UUIVKBHZENILKB-UHFFFAOYSA-N 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 9
- 239000003963 antioxidant agent Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 230000003078 antioxidant effect Effects 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 239000000576 food coloring agent Substances 0.000 claims description 4
- 229910052736 halogen Inorganic materials 0.000 claims description 4
- -1 halogen cyanoacetamides Chemical class 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 3
- 230000003100 immobilizing effect Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000000600 sorbitol Substances 0.000 claims description 3
- 235000010356 sorbitol Nutrition 0.000 claims description 3
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- 239000003826 tablet Substances 0.000 claims description 3
- 239000000080 wetting agent Substances 0.000 claims description 3
- GSOYMOAPJZYXTB-UHFFFAOYSA-N 2,6-ditert-butyl-4-(3,5-ditert-butyl-4-hydroxyphenyl)phenol Chemical class CC(C)(C)C1=C(O)C(C(C)(C)C)=CC(C=2C=C(C(O)=C(C=2)C(C)(C)C)C(C)(C)C)=C1 GSOYMOAPJZYXTB-UHFFFAOYSA-N 0.000 claims description 2
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical class CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- 239000004386 Erythritol Substances 0.000 claims description 2
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 229910052783 alkali metal Inorganic materials 0.000 claims description 2
- 150000001340 alkali metals Chemical class 0.000 claims description 2
- 235000010354 butylated hydroxytoluene Nutrition 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 claims description 2
- 235000019414 erythritol Nutrition 0.000 claims description 2
- 229940009714 erythritol Drugs 0.000 claims description 2
- 239000003205 fragrance Substances 0.000 claims description 2
- 239000002563 ionic surfactant Substances 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 239000002966 varnish Substances 0.000 claims description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- 239000003002 pH adjusting agent Substances 0.000 claims 3
- 239000011780 sodium chloride Substances 0.000 claims 3
- DKCPKDPYUFEZCP-UHFFFAOYSA-N 2,6-di-tert-butylphenol Chemical compound CC(C)(C)C1=CC=CC(C(C)(C)C)=C1O DKCPKDPYUFEZCP-UHFFFAOYSA-N 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 239000007864 aqueous solution Substances 0.000 claims 2
- 239000010692 aromatic oil Substances 0.000 claims 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims 1
- 244000166124 Eucalyptus globulus Species 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- 150000001448 anilines Chemical class 0.000 claims 1
- 239000002585 base Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 235000019253 formic acid Nutrition 0.000 claims 1
- 239000000499 gel Substances 0.000 claims 1
- 239000000049 pigment Substances 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 4
- 244000045947 parasite Species 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 3
- 241000700605 Viruses Species 0.000 abstract description 3
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 2
- 210000000056 organ Anatomy 0.000 abstract description 2
- 230000028327 secretion Effects 0.000 abstract description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 39
- 239000000523 sample Substances 0.000 description 21
- 239000007788 liquid Substances 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 239000000047 product Substances 0.000 description 5
- 231100000331 toxic Toxicity 0.000 description 5
- 230000002588 toxic effect Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 230000000711 cancerogenic effect Effects 0.000 description 4
- 231100000315 carcinogenic Toxicity 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000001263 FEMA 3042 Substances 0.000 description 2
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000006664 bond formation reaction Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003518 caustics Substances 0.000 description 2
- KRVSOGSZCMJSLX-UHFFFAOYSA-L chromic acid Substances O[Cr](O)(=O)=O KRVSOGSZCMJSLX-UHFFFAOYSA-L 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- AWJWCTOOIBYHON-UHFFFAOYSA-N furo[3,4-b]pyrazine-5,7-dione Chemical compound C1=CN=C2C(=O)OC(=O)C2=N1 AWJWCTOOIBYHON-UHFFFAOYSA-N 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 2
- 229940033123 tannic acid Drugs 0.000 description 2
- 235000015523 tannic acid Nutrition 0.000 description 2
- 229920002258 tannic acid Polymers 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- HRTLFDCAIRVTDP-UHFFFAOYSA-N 2-cyanoacetamide 2,2-dibromo-2-cyanoacetamide Chemical compound NC(=O)CC#N.NC(=O)C(Br)(Br)C#N HRTLFDCAIRVTDP-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010015946 Eye irritation Diseases 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000004614 Process Aid Substances 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 150000008051 alkyl sulfates Chemical class 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 239000000538 analytical sample Substances 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000011325 biochemical measurement Methods 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- DGJMPUGMZIKDRO-UHFFFAOYSA-N cyanoacetamide Chemical compound NC(=O)CC#N DGJMPUGMZIKDRO-UHFFFAOYSA-N 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 231100000013 eye irritation Toxicity 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 125000005843 halogen group Chemical group 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000003791 organic solvent mixture Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000003108 parasitologic effect Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 230000015843 photosynthesis, light reaction Effects 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 231100000370 skin sensitisation Toxicity 0.000 description 1
- 229940077386 sodium benzenesulfonate Drugs 0.000 description 1
- MZSDGDXXBZSFTG-UHFFFAOYSA-M sodium;benzenesulfonate Chemical compound [Na+].[O-]S(=O)(=O)C1=CC=CC=C1 MZSDGDXXBZSFTG-UHFFFAOYSA-M 0.000 description 1
- 239000007944 soluble tablet Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229910001631 strontium chloride Inorganic materials 0.000 description 1
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 150000003871 sulfonates Chemical class 0.000 description 1
- 231100000378 teratogenic Toxicity 0.000 description 1
- 230000003390 teratogenic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
シャウディン固定液(水銀塩を含有している)、SAF(ホルムアルデヒドを含有している)、MIF(ホルムアルデヒドと水銀塩とを含有している)、オスミウム酸(発癌性、毒性、苛性である)、クロム酸(発癌性、毒性、苛性)、FA(ホルムアルデヒドとアルコール)および文献に示された他のものが挙げられる。
− 有効な基準(この場合、欧州共同体の基準)に従うものであり、ユーザーおよび環境に対して危険でないか、または危険性はわずかにすぎない(いずれの場合も、従来の固定液よりも危険性が低い)
− 他の形態の危険(可燃性、爆発性、腐食性、環境中への蓄積または高い揮発性)を提示しない
− 穏やかな様式で試料および試料タンパク質と反応し、試料の形態構造の変形(「収縮」)を引き起こさず、従来の液よりも多くの生物学的および生化学的パラメータが損傷を受けずに維持される
− 固定後に試料が受けるべきプロセスに有意な支障(例えば、不要な反応、染色の妨げなど)がない
固定液を提供することからなるものである。
− ハロゲンシアノ−アセトアミド
− 2,2−ジブロモ−2−シアノアセトアミドまたは2,2−ジブロモ−3−ニトリロプロピオンアミドあるいは略してDBNPA
を選択した。
ハロゲンシアノ−アセトアミドおよびDBNPAは強力な殺生物性を有し、試料中の生物学的および微生物学的活性を完全に遮断する
という理由で選択した。
文献から、ハロゲンシアノアセトアミド、特にDBPNAは、溶液中の場合、アルカリ加水分解を受けることが知られている。この溶液で長期間の操作を保証するため、早期劣化を回避するために該溶液に少量の特定の酸化防止剤を添加するのがよい。かくして処理された溶液は、約2年間、その活性が維持される。処分されるときの該溶液の崩壊性は影響を受けず、そのエコロジー的特徴は維持される。
DBPNA 2%
プロピレングリコール 5%
TWEEN80 1%
食品着色料 0.01%
香料混合物 0.01%
酸化防止剤 0.05%
100%になるまで水
DBPNA 100g
ソルビトール粉末 41g
TWEEN80 2.5g
クエン酸 2.0g
食品着色料 1.0g
香料混合物 l.0g
酸化防止剤 2.5g
アラビアガム 50g
全質量 200g
Claims (11)
- 1種類以上のハロゲンシアノアセトアミドが生物学的試料の固定に使用される、生体物質の固定および保存のための固定用物質。
- 2,2−ジブロモ−2−シアノアセトアミドまたは2,2−ジブロモ−3−ニトリロプロピオンアミドあるいは略してDBNPAである、請求項1に記載の固定用物質。
- 水、有機溶媒の有機混合物または水と有機溶媒との混合物であり得る溶媒を含む請求項1または2に記載の固定液。
- 固定液をpH3.0〜pH9.0の範囲に調整するためのpH調整剤を含むことを特徴とする、請求項1、2および3のいずれか1項に記載の固定液。
- 酸、塩基またはバッファーであり得、固定液をpH範囲3.5〜pH6に適合させるためのpH調整剤を含むことを特徴とする、請求項1、2、3および4のいずれか1項に記載の固定液。
- クエン酸、酢酸、リン酸もしくはギ酸またはその対応するバッファーである1種類のpH調整剤の存在を特徴とする、請求項1、2、3、4および5のいずれか1項に記載の固定液。
- 以下のさらなる成分:
− 湿潤剤または湿潤剤混合物:グリセリン、エチレングリコール、プロピレングリコール、エリトリトール、ソルビトールまたは同様の特質を有する物質など
− 界面活性剤または界面活性剤混合物、例えば、TWEEN80などの非イオン性界面活性剤またはドデシル硫酸ナトリウムなどのイオン性界面活性剤
− ユーカリ油などの香気物質または芳香物質の混合物
− 水溶性または溶媒中で使用されるアニリンなどの色素または色素混合物
− 等張性に調整するための塩化ナトリウムなどのアルカリ金属またはアルカリ土類の塩
− 4,4−ビス(2,6−ジ−tert−ブチルフェノール)または2,6−ジ−tert−ブチル−p−クレゾールなどの固定液を安定化させるための酸化防止剤
のうちの少なくとも1種類を含み、これらの成分はすべて適切な溶媒に溶解されている、請求項1、2、3、4、5および6のいずれか1項に記載の固定液。 - 以下の成分:
− 0.05%〜5%の2,2−ジブロモ−2−シアノアセトアミドまたは2,2−ジブロモ−3−ニトリロプロピオンアミドあるいは略してDBNPA、クエン酸、1%〜30%のプロピレングリコール、0.01%〜5%のTween80、0.001〜0.1%の芳香油、0.001%〜0.1%の食用色素、0.05%〜0.1%の酸化防止剤4.4−ビス(2,6−ジ−tert−ブチルフェノール)、0.01〜10%の塩化ナトリウム
を含む水溶液からなる、請求項1ないし7のいずれか1項に記載の固定液。 - 以下の成分:
− 1%の2,2−ジブロモ−2−シアノアセトアミドまたは2,2−ジブロモ−3−ニトリロプロピオンアミド、0.5%のクエン酸、12%のプロピレングリコール、0.1%のTween80、0.05%の芳香油、0.05%の酸化防止剤4.4−ビス(2,6−ジ−tert−ブチルフェノール)、0.01%の食用色素、0.1%の塩化ナトリウム
を含む水溶液からなる、請求項1ないし8のいずれか1項に記載の固定液。 - 諸成分が、溶媒なしで粉末、タブレット、カプセル、ゲルもしくはワニスの形態で、またはわずかな溶媒を伴って濃縮物の形態で存在し、必要量の溶媒の添加によりすぐに使える状態の溶液が得られるものである、請求項1ないし9のいずれか1項に記載の濃縮型固定液。
- 固定をより良好に調節するための他の固定剤または固定用物質と合わせたものであり得る、請求項1ないし10のいずれか1項に記載の固定液。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/IB2011/051937 WO2012150479A1 (en) | 2011-05-02 | 2011-05-02 | Fixative solution, for fixation and preservation of biological samples |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2014517273A true JP2014517273A (ja) | 2014-07-17 |
JP6177764B2 JP6177764B2 (ja) | 2017-08-09 |
Family
ID=44741688
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2014508879A Expired - Fee Related JP6177764B2 (ja) | 2011-05-02 | 2011-05-02 | 生物学的試料の固定および保存のための固定液 |
Country Status (10)
Country | Link |
---|---|
US (1) | US9677978B2 (ja) |
EP (1) | EP2705345B1 (ja) |
JP (1) | JP6177764B2 (ja) |
CN (1) | CN103688152B (ja) |
BR (1) | BR112013028298B1 (ja) |
CA (1) | CA2834817C (ja) |
ES (1) | ES2537173T3 (ja) |
MX (1) | MX361688B (ja) |
PT (1) | PT2705345E (ja) |
WO (1) | WO2012150479A1 (ja) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9677978B2 (en) | 2011-05-02 | 2017-06-13 | José Carlos Lapenna | Fixative solution, for fixation and preservation of biological samples |
CN105486554A (zh) * | 2015-11-19 | 2016-04-13 | 新疆农业大学 | 一种蜱虫样品扫描电镜固定配方及方法 |
CN107367417B (zh) * | 2017-07-14 | 2021-06-22 | 上海市松江区中心医院 | 一种超声引导下穿刺细胞快速固定剂组合物及其制备方法 |
CN108931408B (zh) * | 2018-06-15 | 2021-08-27 | 山东聚众数字医学科技开发有限公司 | 一种解剖教学标本用保色固定液及其制备方法 |
CN109090096A (zh) * | 2018-09-28 | 2018-12-28 | 河南科技大学 | 一种鱼内脏塑化标本的制作方法 |
CN109042622A (zh) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | 一种保色固定剂及其制备方法与在标本制备中的应用 |
CN109166439B (zh) * | 2018-10-30 | 2020-11-13 | 河南科技大学 | 一种羊的胃剖面硅胶模型的制作方法 |
CN109511653A (zh) * | 2018-11-28 | 2019-03-26 | 陈大为 | 植物提取防dna和rna降解并保持细胞原有形态的保存液 |
CN110455601A (zh) * | 2019-06-28 | 2019-11-15 | 魏兴江 | 一种含有异噻唑啉酮的组合物的用途 |
CN110283726A (zh) * | 2019-07-03 | 2019-09-27 | 孝感宏翔生物医械技术有限公司 | 一种液基细胞和微生物处理保存试剂及其制备方法 |
CN111397997A (zh) * | 2020-04-26 | 2020-07-10 | 中烨(山东)检验检测有限公司 | 一种用于新鲜组织样本固定的组织固定液 |
EP4145105A1 (de) | 2021-09-06 | 2023-03-08 | Anacyte Laboratories GmbH | Lösung für die asservierung von zellen |
Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4241080A (en) * | 1979-05-29 | 1980-12-23 | The Dow Chemical Company | Stabilized aqueous antimicrobial composition |
JPS5947601U (ja) * | 1982-09-18 | 1984-03-29 | 余村 道雄 | 二枚貝の固定構造 |
JPH0624910A (ja) * | 1992-02-17 | 1994-02-01 | Katayama Chem Works Co Ltd | 殺菌剤 |
JPH10324604A (ja) * | 1997-05-22 | 1998-12-08 | Hokko Chem Ind Co Ltd | 防腐防かび剤 |
JP2000506893A (ja) * | 1996-03-20 | 2000-06-06 | ザ・ダウ・ケミカル・カンパニー | 2,2―ジブロモ―3―ニトリロプロピオンアミドの懸濁配合物 |
JP2000198705A (ja) * | 1999-01-07 | 2000-07-18 | Rohm & Haas Co | 殺生物組成物 |
JP3071306U (ja) * | 2000-02-25 | 2000-08-29 | 株式会社フジコン | 昆虫標本作成キット |
JP2000336038A (ja) * | 1999-03-19 | 2000-12-05 | Pola Chem Ind Inc | 肝臓保護剤 |
US20050020649A1 (en) * | 2003-07-24 | 2005-01-27 | Williams Terry Michael | Microbicidal composition |
JP2006513162A (ja) * | 2002-11-01 | 2006-04-20 | パラテック ファーマシューティカルズ インコーポレイテッド | 転写因子調節化合物およびその使用方法 |
US20080102094A1 (en) * | 2006-11-01 | 2008-05-01 | Bryant Stephen D | Microbicidal Compositions Including A Cyanodithiocarbimate And A Second Microbicide, And Methods of Using The Same |
US20100186631A1 (en) * | 2009-01-29 | 2010-07-29 | Thor Gmbh | Compounds for preventing ghost odour |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3689660A (en) * | 1970-07-31 | 1972-09-05 | Dow Chemical Co | Stable liquid antimicrobial composition |
GB1560481A (en) | 1975-07-09 | 1980-02-06 | Johnson Matthey Co Ltd | Complexes of osmium tetroxide |
US4163797A (en) * | 1977-12-14 | 1979-08-07 | The Dow Chemical Company | Stabilized aqueous amide antimicrobial composition |
DE3822183A1 (de) | 1988-06-28 | 1990-02-08 | Schubert Werner | Substanzgruppe fuer fixierungszwecke |
DE3824936A1 (de) | 1988-06-28 | 1990-03-22 | Schubert Werner | Schnellfixierung |
US5422277A (en) | 1992-03-27 | 1995-06-06 | Ortho Diagnostic Systems Inc. | Cell fixative composition and method of staining cells without destroying the cell surface |
DE4404544A1 (de) | 1994-02-12 | 1995-08-17 | Schubert Werner | Verfahren und Vorrichtung für die Stabilisation frischer Gewebs-Probeexzisionen |
JPH09278700A (ja) * | 1996-04-17 | 1997-10-28 | Katayama Chem Works Co Ltd | 脂肪族アルデヒド水性溶液、その安定剤及び安定化方法 |
AU7865598A (en) * | 1997-08-14 | 1999-02-25 | Rohm And Haas Company | Solid biocidal compositions |
EP0953284A1 (en) * | 1998-04-24 | 1999-11-03 | Clearwater, Inc. | Biocide with accelerated dissolution |
MXPA01000215A (es) | 1998-06-30 | 2003-09-10 | Lamina Inc | Composicion fijadora histologica y metodos de uso. |
DE60136573D1 (de) | 2001-09-28 | 2008-12-24 | Dcs Innovative Diagnostik Syst | Gewebefixativzusammensetzung |
EP1455174B1 (en) | 2003-03-05 | 2004-12-15 | Milestone S.r.l. | Fixative |
US7326577B2 (en) | 2003-10-20 | 2008-02-05 | Esoterix, Inc. | Cell fixation and use in phospho-proteome screening |
US9677978B2 (en) | 2011-05-02 | 2017-06-13 | José Carlos Lapenna | Fixative solution, for fixation and preservation of biological samples |
-
2011
- 2011-05-02 US US14/115,141 patent/US9677978B2/en not_active Expired - Fee Related
- 2011-05-02 EP EP11764613.3A patent/EP2705345B1/en not_active Not-in-force
- 2011-05-02 WO PCT/IB2011/051937 patent/WO2012150479A1/en active Application Filing
- 2011-05-02 ES ES11764613.3T patent/ES2537173T3/es active Active
- 2011-05-02 JP JP2014508879A patent/JP6177764B2/ja not_active Expired - Fee Related
- 2011-05-02 MX MX2013012647A patent/MX361688B/es active IP Right Grant
- 2011-05-02 BR BR112013028298-3A patent/BR112013028298B1/pt not_active IP Right Cessation
- 2011-05-02 CA CA2834817A patent/CA2834817C/en not_active Expired - Fee Related
- 2011-05-02 PT PT117646133T patent/PT2705345E/pt unknown
- 2011-05-02 CN CN201180071839.9A patent/CN103688152B/zh not_active Expired - Fee Related
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4241080A (en) * | 1979-05-29 | 1980-12-23 | The Dow Chemical Company | Stabilized aqueous antimicrobial composition |
JPS5947601U (ja) * | 1982-09-18 | 1984-03-29 | 余村 道雄 | 二枚貝の固定構造 |
JPH0624910A (ja) * | 1992-02-17 | 1994-02-01 | Katayama Chem Works Co Ltd | 殺菌剤 |
JP2000506893A (ja) * | 1996-03-20 | 2000-06-06 | ザ・ダウ・ケミカル・カンパニー | 2,2―ジブロモ―3―ニトリロプロピオンアミドの懸濁配合物 |
JPH10324604A (ja) * | 1997-05-22 | 1998-12-08 | Hokko Chem Ind Co Ltd | 防腐防かび剤 |
JP2000198705A (ja) * | 1999-01-07 | 2000-07-18 | Rohm & Haas Co | 殺生物組成物 |
JP2000336038A (ja) * | 1999-03-19 | 2000-12-05 | Pola Chem Ind Inc | 肝臓保護剤 |
JP3071306U (ja) * | 2000-02-25 | 2000-08-29 | 株式会社フジコン | 昆虫標本作成キット |
JP2006513162A (ja) * | 2002-11-01 | 2006-04-20 | パラテック ファーマシューティカルズ インコーポレイテッド | 転写因子調節化合物およびその使用方法 |
US20050020649A1 (en) * | 2003-07-24 | 2005-01-27 | Williams Terry Michael | Microbicidal composition |
US20080102094A1 (en) * | 2006-11-01 | 2008-05-01 | Bryant Stephen D | Microbicidal Compositions Including A Cyanodithiocarbimate And A Second Microbicide, And Methods of Using The Same |
US20100186631A1 (en) * | 2009-01-29 | 2010-07-29 | Thor Gmbh | Compounds for preventing ghost odour |
Also Published As
Publication number | Publication date |
---|---|
CN103688152A (zh) | 2014-03-26 |
BR112013028298A2 (pt) | 2018-10-09 |
ES2537173T3 (es) | 2015-06-03 |
CN103688152B (zh) | 2017-10-20 |
EP2705345A1 (en) | 2014-03-12 |
WO2012150479A1 (en) | 2012-11-08 |
CA2834817A1 (en) | 2012-11-08 |
MX2013012647A (es) | 2015-11-16 |
US9677978B2 (en) | 2017-06-13 |
JP6177764B2 (ja) | 2017-08-09 |
CA2834817C (en) | 2019-01-15 |
US20140162246A1 (en) | 2014-06-12 |
PT2705345E (pt) | 2015-06-17 |
MX361688B (es) | 2018-12-13 |
BR112013028298B1 (pt) | 2020-11-17 |
EP2705345B1 (en) | 2015-02-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6177764B2 (ja) | 生物学的試料の固定および保存のための固定液 | |
Halla et al. | Cosmetics preservation: a review on present strategies | |
Ince et al. | Thymoquinone attenuates cypermethrin induced oxidative stress in Swiss albino mice | |
TW201402008A (zh) | 殺菌劑組成物 | |
Fernández‐Serrano et al. | Ecotoxicological assessment of mixtures of ether carboxylic derivative and amine‐oxide‐based non‐ionic surfactants on the aquatic environment | |
US11656159B2 (en) | Composition for preparing biological material having excellent light transmissivity and use of composition | |
Lee et al. | The influence of alkane chain length on the skin irritation potential of 1, 2‐alkanediols | |
CN110292045A (zh) | 一种含有ε-聚赖氨酸的抗微生物组合物制备方法及应用 | |
CN105532637A (zh) | 一种人体教学标本保存液及其制备方法 | |
CN106706395B (zh) | 一种环保固定液 | |
CN106726869A (zh) | 一种天然无刺激的洗发水及其制备方法 | |
KR101924401B1 (ko) | 구강상피세포 보존액과 이의 제조방법 | |
AU2012264488A8 (en) | Aqueous alcoholic microbicidal compositions comprising copper ions | |
CN110200899A (zh) | 一种防腐剂及其制备方法和应用 | |
US7915007B2 (en) | Fixative for fixing biological materials | |
JP6887991B2 (ja) | 化粧品保存用液体濃縮物 | |
JP5372311B2 (ja) | モノアルキルグリセロールエーテルおよび芳香族アルコールに基づく安定化剤組成物 | |
CN103674640A (zh) | 甲醛分子膜化缓冲液及其制备方法 | |
JP2008533086A (ja) | 有効性の改善されたイソチアゾロン含有保存剤。 | |
Melrose et al. | Histological and immunohistological studies on cartilage | |
CN109432157A (zh) | 一种皮肤抑菌液及其制备方法 | |
TW200817043A (en) | Skin preparation for external use | |
EP3214180B1 (en) | Methods, compositions and kits for determining cleanness of a surface | |
CN110122498A (zh) | 一种酸碱体系下的控菌复配杀菌剂及其制备方法 | |
US20220142147A1 (en) | Compositions and Methods for Preservation and Fixation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140501 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20141217 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150106 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150403 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150430 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150603 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150703 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20151208 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20160307 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20160407 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20160506 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160608 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20160608 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160629 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20160711 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20161130 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170227 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170531 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20170612 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20170712 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6177764 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |