JP2014014371A5 - - Google Patents
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- JP2014014371A5 JP2014014371A5 JP2013185104A JP2013185104A JP2014014371A5 JP 2014014371 A5 JP2014014371 A5 JP 2014014371A5 JP 2013185104 A JP2013185104 A JP 2013185104A JP 2013185104 A JP2013185104 A JP 2013185104A JP 2014014371 A5 JP2014014371 A5 JP 2014014371A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- oligonucleotide
- rnase
- nucleic acid
- cleavage domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000013615 primer Substances 0.000 claims 130
- 108091034117 Oligonucleotide Proteins 0.000 claims 108
- 238000003776 cleavage reaction Methods 0.000 claims 85
- 230000007017 scission Effects 0.000 claims 84
- 238000000034 method Methods 0.000 claims 79
- 230000000903 blocking effect Effects 0.000 claims 61
- 150000007523 nucleic acids Chemical class 0.000 claims 58
- 108010083644 Ribonucleases Proteins 0.000 claims 56
- 102000006382 Ribonucleases Human genes 0.000 claims 56
- 108020004707 nucleic acids Proteins 0.000 claims 44
- 102000039446 nucleic acids Human genes 0.000 claims 44
- 102000004190 Enzymes Human genes 0.000 claims 35
- 108090000790 Enzymes Proteins 0.000 claims 35
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 34
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 27
- 239000011541 reaction mixture Substances 0.000 claims 27
- 239000000523 sample Substances 0.000 claims 24
- 230000000295 complement effect Effects 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 16
- 239000000758 substrate Substances 0.000 claims 13
- 102100034343 Integrase Human genes 0.000 claims 12
- 230000004543 DNA replication Effects 0.000 claims 11
- 239000002773 nucleotide Substances 0.000 claims 11
- 229920000642 polymer Polymers 0.000 claims 10
- 101710203526 Integrase Proteins 0.000 claims 9
- 150000001875 compounds Chemical class 0.000 claims 9
- 238000012986 modification Methods 0.000 claims 9
- 230000004048 modification Effects 0.000 claims 9
- 238000006243 chemical reaction Methods 0.000 claims 8
- 229920002477 rna polymer Polymers 0.000 claims 8
- 125000006850 spacer group Chemical group 0.000 claims 8
- 102000003960 Ligases Human genes 0.000 claims 7
- 108090000364 Ligases Proteins 0.000 claims 7
- 125000000217 alkyl group Chemical group 0.000 claims 7
- -1 polymerase Proteins 0.000 claims 7
- 125000000446 sulfanediyl group Chemical group *S* 0.000 claims 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 6
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 6
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 5
- 230000000694 effects Effects 0.000 claims 5
- 238000009396 hybridization Methods 0.000 claims 5
- 239000002751 oligonucleotide probe Substances 0.000 claims 5
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 4
- 239000005977 Ethylene Substances 0.000 claims 4
- 150000001412 amines Chemical class 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 125000001369 canonical nucleoside group Chemical group 0.000 claims 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 4
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 4
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 claims 4
- 230000035772 mutation Effects 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 claims 4
- 239000001226 triphosphate Substances 0.000 claims 4
- 235000011178 triphosphate Nutrition 0.000 claims 4
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 3
- 229930024421 Adenine Natural products 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- 229960000643 adenine Drugs 0.000 claims 3
- 229940104302 cytosine Drugs 0.000 claims 3
- 239000007850 fluorescent dye Substances 0.000 claims 3
- 125000001153 fluoro group Chemical group F* 0.000 claims 3
- 239000000203 mixture Substances 0.000 claims 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 3
- 239000002987 primer (paints) Substances 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical class CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims 2
- 241001148023 Pyrococcus abyssi Species 0.000 claims 2
- 241000205156 Pyrococcus furiosus Species 0.000 claims 2
- 108091028664 Ribonucleotide Proteins 0.000 claims 2
- 241000205091 Sulfolobus solfataricus Species 0.000 claims 2
- 241001235254 Thermococcus kodakarensis Species 0.000 claims 2
- 238000003556 assay Methods 0.000 claims 2
- 150000001768 cations Chemical class 0.000 claims 2
- 230000001351 cycling effect Effects 0.000 claims 2
- 239000005546 dideoxynucleotide Substances 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 239000002777 nucleoside Substances 0.000 claims 2
- 239000002336 ribonucleotide Substances 0.000 claims 2
- 125000002652 ribonucleotide group Chemical group 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- 230000002194 synthesizing effect Effects 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 108700028369 Alleles Proteins 0.000 claims 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims 1
- 239000003155 DNA primer Substances 0.000 claims 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 1
- 241000194036 Lactococcus Species 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 241000203407 Methanocaldococcus jannaschii Species 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
- ZADPBFCGQRWHPN-UHFFFAOYSA-N boronic acid Chemical compound OBO ZADPBFCGQRWHPN-UHFFFAOYSA-N 0.000 claims 1
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 1
- 239000005547 deoxyribonucleotide Substances 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 150000005690 diesters Chemical class 0.000 claims 1
- NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 claims 1
- 239000000975 dye Substances 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 claims 1
- 238000006460 hydrolysis reaction Methods 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims 1
- NBTOZLQBSIZIKS-UHFFFAOYSA-N methoxide Chemical compound [O-]C NBTOZLQBSIZIKS-UHFFFAOYSA-N 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 229910052759 nickel Inorganic materials 0.000 claims 1
- 125000003835 nucleoside group Chemical group 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 125000001981 tert-butyldimethylsilyl group Chemical group [H]C([H])([H])[Si]([H])(C([H])([H])[H])[*]C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H] 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
- 125000000025 triisopropylsilyl group Chemical group C(C)(C)[Si](C(C)C)(C(C)C)* 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4920408P | 2008-04-30 | 2008-04-30 | |
| US61/049,204 | 2008-04-30 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011507669A Division JP5539325B2 (ja) | 2008-04-30 | 2009-04-30 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015116458A Division JP2015221036A (ja) | 2008-04-30 | 2015-06-09 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014014371A JP2014014371A (ja) | 2014-01-30 |
| JP2014014371A5 true JP2014014371A5 (https=) | 2014-06-26 |
Family
ID=41203821
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011507669A Active JP5539325B2 (ja) | 2008-04-30 | 2009-04-30 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2013185104A Pending JP2014014371A (ja) | 2008-04-30 | 2013-09-06 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2015116458A Ceased JP2015221036A (ja) | 2008-04-30 | 2015-06-09 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246235A Active JP6453296B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246236A Expired - Fee Related JP6276835B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2018038239A Active JP6510695B2 (ja) | 2008-04-30 | 2018-03-05 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011507669A Active JP5539325B2 (ja) | 2008-04-30 | 2009-04-30 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015116458A Ceased JP2015221036A (ja) | 2008-04-30 | 2015-06-09 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246235A Active JP6453296B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246236A Expired - Fee Related JP6276835B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2018038239A Active JP6510695B2 (ja) | 2008-04-30 | 2018-03-05 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Country Status (7)
| Country | Link |
|---|---|
| US (4) | US20090325169A1 (https=) |
| EP (6) | EP2644708B1 (https=) |
| JP (6) | JP5539325B2 (https=) |
| AU (1) | AU2009242546B2 (https=) |
| CA (1) | CA2722541C (https=) |
| DK (2) | DK2279263T3 (https=) |
| WO (1) | WO2009135093A2 (https=) |
Families Citing this family (79)
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| US9850527B2 (en) | 2010-06-14 | 2017-12-26 | National University Of Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative PCR |
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| US20120052482A1 (en) * | 2010-08-27 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting hepatitis c virus and method of detecting hepatitis c virus using the same |
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| EP2663654A1 (en) * | 2011-01-14 | 2013-11-20 | Life Technologies Corporation | Methods, compositions, and kits for detecting rare cells |
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2009
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- 2009-04-30 EP EP09739895.2A patent/EP2279263B1/en active Active
- 2009-04-30 DK DK09739895.2T patent/DK2279263T3/da active
- 2009-04-30 AU AU2009242546A patent/AU2009242546B2/en active Active
- 2009-04-30 EP EP13173388.3A patent/EP2644707B1/en active Active
- 2009-04-30 EP EP13173390.9A patent/EP2644709B1/en active Active
- 2009-04-30 DK DK13173388.3T patent/DK2644707T3/en active
- 2009-04-30 EP EP16198184.0A patent/EP3150727B1/en active Active
- 2009-04-30 CA CA2722541A patent/CA2722541C/en active Active
- 2009-04-30 WO PCT/US2009/042454 patent/WO2009135093A2/en not_active Ceased
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- 2009-04-30 EP EP13173391.7A patent/EP2644710B1/en active Active
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- 2016-12-20 JP JP2016246236A patent/JP6276835B2/ja not_active Expired - Fee Related
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