DE60139762D1 - Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren - Google Patents

Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren

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Publication number
DE60139762D1
DE60139762D1 DE60139762T DE60139762T DE60139762D1 DE 60139762 D1 DE60139762 D1 DE 60139762D1 DE 60139762 T DE60139762 T DE 60139762T DE 60139762 T DE60139762 T DE 60139762T DE 60139762 D1 DE60139762 D1 DE 60139762D1
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DE
Germany
Prior art keywords
pyrophosphorolysis
pap
allel
sequencing
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE60139762T
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English (en)
Inventor
Qiang Liu
Steve S Sommer
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City of Hope
Original Assignee
City of Hope
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Publication date
Application filed by City of Hope filed Critical City of Hope
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Publication of DE60139762D1 publication Critical patent/DE60139762D1/de
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
DE60139762T 2000-02-23 2001-02-22 Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren Expired - Lifetime DE60139762D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18431500P 2000-02-23 2000-02-23
US18703500P 2000-03-06 2000-03-06
US23718000P 2000-10-03 2000-10-03
PCT/US2001/005527 WO2001062975A2 (en) 2000-02-23 2001-02-22 Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination

Publications (1)

Publication Number Publication Date
DE60139762D1 true DE60139762D1 (de) 2009-10-15

Family

ID=27391815

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60139762T Expired - Lifetime DE60139762D1 (de) 2000-02-23 2001-02-22 Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren

Country Status (9)

Country Link
US (2) US6534269B2 (de)
EP (2) EP1259646B1 (de)
JP (1) JP4989004B2 (de)
AT (1) ATE441726T1 (de)
AU (2) AU4162101A (de)
CA (1) CA2395391C (de)
DE (1) DE60139762D1 (de)
ES (1) ES2330408T3 (de)
WO (1) WO2001062975A2 (de)

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ATE552349T1 (de) * 2002-05-10 2012-04-15 Hope City Pyrophosphorolyse-aktivierte polymerisation (pap)
WO2005086679A2 (en) 2004-03-05 2005-09-22 Medical College Of Ohio Methods and compositions for assessing nucleic acids and alleles
CA2568238C (en) * 2004-04-12 2013-10-15 Medical College Of Ohio Methods and compositions for assaying analytes
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US20060060977A1 (en) * 2004-09-22 2006-03-23 Kabushiki Kaisha Toshiba Semiconductor device
US7378260B2 (en) * 2005-04-01 2008-05-27 Applera Corporation Products and methods for reducing dye artifacts
US8071536B2 (en) * 2007-11-28 2011-12-06 Roche Molecular System, Inc. Mutant DNA polymerases with improved pyrophosphorolysis activated polymerization (PAP) ability
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
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CN101381766B (zh) * 2008-06-11 2012-04-18 厦门大学 一种基因稀有突变的定量检测方法
US8481685B2 (en) * 2008-11-03 2013-07-09 Kapa Biosystems Modified DNA polymerases
US20100112565A1 (en) * 2008-11-03 2010-05-06 Life Technologies Corporation Methods, kits, and reaction mixtures for high resolution melt genotyping
EP2652152A4 (de) * 2010-12-13 2014-06-25 Life Technologies Corp Polymerisation von nukleinsäuren mittels aktivierung durch polyphosphorolyse-reaktionen (app)
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2017072678A1 (en) 2015-10-26 2017-05-04 Oti Lumionics Inc. Method for patterning a coating on a surface and device including a patterned coating
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
CN105316421B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 用于检测与肺癌相关的shox2基因启动子区甲基化程度的试剂盒
CN116583131A (zh) 2017-04-26 2023-08-11 Oti照明公司 用于图案化表面上覆层的方法和包括图案化覆层的装置
KR20200006569A (ko) 2017-05-17 2020-01-20 오티아이 루미오닉스 인크. 패턴화 코팅 위에 전도성 코팅을 선택적으로 증착시키는 방법 및 전도성 코팅을 포함하는 디바이스
CN110603326B (zh) * 2017-09-26 2024-03-08 宁波尚宁生物技术有限公司 扩增靶核酸的方法
US11751415B2 (en) 2018-02-02 2023-09-05 Oti Lumionics Inc. Materials for forming a nucleation-inhibiting coating and devices incorporating same
WO2019180137A1 (en) * 2018-03-21 2019-09-26 F. Hoffmann-La Roche Ag Dna polymerases for efficient and effective incorporation of methylated-dntps
US11268140B2 (en) * 2018-06-12 2022-03-08 Shaofeng Ding Delayed pyrophosphorolysis activated polymerization
US11730012B2 (en) 2019-03-07 2023-08-15 Oti Lumionics Inc. Materials for forming a nucleation-inhibiting coating and devices incorporating same
WO2020261191A1 (en) 2019-06-26 2020-12-30 Oti Lumionics Inc. Optoelectronic device including light transmissive regions, with light diffraction characteristics
US11832473B2 (en) 2019-06-26 2023-11-28 Oti Lumionics Inc. Optoelectronic device including light transmissive regions, with light diffraction characteristics
JP2022544198A (ja) 2019-08-09 2022-10-17 オーティーアイ ルミオニクス インコーポレーテッド 補助電極および仕切りを含む光電子デバイス

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Also Published As

Publication number Publication date
EP1259646B1 (de) 2009-09-02
ATE441726T1 (de) 2009-09-15
CA2395391C (en) 2011-08-02
JP2003526344A (ja) 2003-09-09
WO2001062975A2 (en) 2001-08-30
EP2112233B1 (de) 2013-10-23
US20020127554A1 (en) 2002-09-12
CA2395391A1 (en) 2001-08-30
WO2001062975A9 (en) 2002-10-24
WO2001062975A3 (en) 2002-08-29
EP2112233A1 (de) 2009-10-28
US7105298B2 (en) 2006-09-12
ES2330408T3 (es) 2009-12-10
EP1259646A2 (de) 2002-11-27
US20030092051A1 (en) 2003-05-15
AU2001241621B2 (en) 2005-08-25
US6534269B2 (en) 2003-03-18
AU4162101A (en) 2001-09-03
JP4989004B2 (ja) 2012-08-01

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