JP2009533033A5 - - Google Patents

Info

Publication number

JP2009533033A5

JP2009533033A5 JP2009504634A JP2009504634A JP2009533033A5 JP 2009533033 A5 JP2009533033 A5 JP 2009533033A5 JP 2009504634 A JP2009504634 A JP 2009504634A JP 2009504634 A JP2009504634 A JP 2009504634A JP 2009533033 A5 JP2009533033 A5 JP 2009533033A5

Authority

JP

Japan

Prior art keywords

gdh

mutant

glucose

variant

improve

Prior art date

2007-04-11

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 101000806049 Acinetobacter calcoaceticus Quinoprotein glucose dehydrogenase B Proteins 0.000 claims 13
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 10
• 239000008103 glucose Substances 0.000 claims 10
• 230000035772 mutation Effects 0.000 claims 6
• 238000006467 substitution reaction Methods 0.000 claims 5
• OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims 4
• GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims 4
• 238000004519 manufacturing process Methods 0.000 claims 4
• 239000013604 expression vector Substances 0.000 claims 3
• 238000000034 method Methods 0.000 claims 3
• 102000040430 polynucleotide Human genes 0.000 claims 3
• 108091033319 polynucleotide Proteins 0.000 claims 3
• 239000002157 polynucleotide Substances 0.000 claims 3
• 102000004190 Enzymes Human genes 0.000 claims 2
• 108090000790 Enzymes Proteins 0.000 claims 2
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
• 235000001014 amino acid Nutrition 0.000 claims 2
• 150000001413 amino acids Chemical group 0.000 claims 2
• 238000005259 measurement Methods 0.000 claims 2
• 238000010647 peptide synthesis reaction Methods 0.000 claims 2
• 230000001737 promoting effect Effects 0.000 claims 2
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims 1
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 241000588624 Acinetobacter calcoaceticus Species 0.000 claims 1
• 239000004471 Glycine Substances 0.000 claims 1
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 1
• 102000008300 Mutant Proteins Human genes 0.000 claims 1
• 108010021466 Mutant Proteins Proteins 0.000 claims 1
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 1
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 1
• 239000004473 Threonine Substances 0.000 claims 1
• 102220474575 Ubiquitin-conjugating enzyme E2 D1_D87R_mutation Human genes 0.000 claims 1
• 235000004279 alanine Nutrition 0.000 claims 1
• 239000003153 chemical reaction reagent Substances 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 230000001419 dependent effect Effects 0.000 claims 1
• 238000001514 detection method Methods 0.000 claims 1
• 108040005045 glucose dehydrogenase activity proteins Proteins 0.000 claims 1
• 238000003780 insertion Methods 0.000 claims 1
• 230000037431 insertion Effects 0.000 claims 1
• 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 claims 1