JP2013078329A5 - - Google Patents
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- Publication number
- JP2013078329A5 JP2013078329A5 JP2012264713A JP2012264713A JP2013078329A5 JP 2013078329 A5 JP2013078329 A5 JP 2013078329A5 JP 2012264713 A JP2012264713 A JP 2012264713A JP 2012264713 A JP2012264713 A JP 2012264713A JP 2013078329 A5 JP2013078329 A5 JP 2013078329A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- dehalogenase
- mutant dehalogenase
- mutant
- substitution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006467 substitution reaction Methods 0.000 claims 14
- 238000000034 method Methods 0.000 claims 12
- 239000000758 substrate Substances 0.000 claims 9
- 125000003275 alpha amino acid group Chemical group 0.000 claims 7
- 125000000524 functional group Chemical group 0.000 claims 6
- 230000004927 fusion Effects 0.000 claims 6
- 229920001184 polypeptide Polymers 0.000 claims 5
- 102000004196 processed proteins & peptides Human genes 0.000 claims 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 102220539256 Programmed cell death 1 ligand 2_S58T_mutation Human genes 0.000 claims 3
- 102200076247 c.463G>A Human genes 0.000 claims 3
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 102200094897 rs121913558 Human genes 0.000 claims 3
- 102220024940 rs199473371 Human genes 0.000 claims 3
- 239000007787 solid Substances 0.000 claims 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 2
- 102220466696 Histone H4 transcription factor_L88M_mutation Human genes 0.000 claims 2
- 102220468699 Protein arginine N-methyltransferase 3_Y87F_mutation Human genes 0.000 claims 2
- 102220512952 Putative 60S ribosomal protein L13a protein RPL13AP3_K195N_mutation Human genes 0.000 claims 2
- 230000004913 activation Effects 0.000 claims 2
- 125000004432 carbon atom Chemical group C* 0.000 claims 2
- 150000002148 esters Chemical class 0.000 claims 2
- 102200010676 rs137853070 Human genes 0.000 claims 2
- 102200004222 rs199473453 Human genes 0.000 claims 2
- 102220037374 rs202045056 Human genes 0.000 claims 2
- 102220105417 rs879254506 Human genes 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical group [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- 125000004429 atom Chemical group 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Chemical group BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 claims 1
- 229910052794 bromium Chemical group 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 125000001309 chloro group Chemical group Cl* 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 229910052736 halogen Inorganic materials 0.000 claims 1
- 150000002367 halogens Chemical group 0.000 claims 1
- 125000001072 heteroaryl group Chemical group 0.000 claims 1
- 125000004356 hydroxy functional group Chemical group O* 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 150000002978 peroxides Chemical class 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85523706P | 2006-10-30 | 2006-10-30 | |
| US60/855,237 | 2006-10-30 | ||
| US93020107P | 2007-05-15 | 2007-05-15 | |
| US60/930,201 | 2007-05-15 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009534717A Division JP5680302B2 (ja) | 2006-10-30 | 2007-10-30 | キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013078329A JP2013078329A (ja) | 2013-05-02 |
| JP2013078329A5 true JP2013078329A5 (enExample) | 2013-10-03 |
| JP5840117B2 JP5840117B2 (ja) | 2016-01-06 |
Family
ID=39344927
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009534717A Active JP5680302B2 (ja) | 2006-10-30 | 2007-10-30 | キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質 |
| JP2012264713A Active JP5840117B2 (ja) | 2006-10-30 | 2012-12-03 | キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009534717A Active JP5680302B2 (ja) | 2006-10-30 | 2007-10-30 | キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質 |
Country Status (5)
| Country | Link |
|---|---|
| US (5) | US8420367B2 (enExample) |
| EP (4) | EP2492342B1 (enExample) |
| JP (2) | JP5680302B2 (enExample) |
| CA (1) | CA2667697A1 (enExample) |
| WO (1) | WO2008054821A2 (enExample) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1594962B1 (en) | 2003-01-31 | 2014-08-27 | Promega Corporation | Covalent tethering of functional groups to proteins |
| US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
| US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
| CA2667697A1 (en) | 2006-10-30 | 2008-05-08 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and functional expression |
| EP2635583B1 (en) | 2010-11-02 | 2015-06-03 | Promega Corporation | Coelenterazine derivatives and methods of using same |
| AU2011323418B2 (en) | 2010-11-02 | 2017-07-27 | Promega Corporation | Novel coelenterazine substrates and methods of use |
| JP6297559B2 (ja) | 2012-07-31 | 2018-03-20 | バジル・エム・ハンタッシュＢａｓｉｌ Ｍ． ＨＡＮＴＡＳＨ | Ｈｌａｇ改変された細胞および方法 |
| US11072811B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| US9927430B2 (en) | 2014-01-29 | 2018-03-27 | Promega Corporation | Pro-substrates for live cell applications |
| JP6703484B2 (ja) | 2014-01-29 | 2020-06-03 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ |
| KR20190025362A (ko) * | 2017-09-01 | 2019-03-11 | 삼성전자주식회사 | 할로산 데할로게나제 수퍼패밀리 단백질 변이체 및 그를 이용한 시료 중 불소 함유 화합물의 농도를 감소시키는 방법 |
| IL280880B2 (en) | 2018-08-27 | 2025-04-01 | Regeneron Pharma | Using Raman Spectroscopy in Downstream Purification |
| EP3932934A4 (en) * | 2019-02-27 | 2023-09-20 | National University Corporation Tokyo Medical and Dental University | FUSION PROTEIN OF AN ANTIGEN BINDING PROTEIN AND FLUORESCENT PROTEIN OR FLUORESCENCE-LABLED TAG PROTEIN |
| CN110819612A (zh) * | 2019-11-29 | 2020-02-21 | 天津科技大学 | 新型抗自切碱性蛋白酶的筛选 |
| WO2022160156A1 (en) | 2021-01-28 | 2022-08-04 | Genequantum Healthcare (Suzhou) Co., Ltd. | Ligase fusion proteins and applications thereof |
| JP2022127607A (ja) * | 2021-02-19 | 2022-08-31 | 国立研究開発法人産業技術総合研究所 | 標的タンパク質の生産方法 |
| CN115322980B (zh) * | 2021-05-11 | 2024-04-02 | 中国科学院化学研究所 | 酰胺水解酶突变体及其在手性环状化合物合成上的应用 |
| EP4206674B1 (en) | 2021-12-28 | 2025-10-22 | Encodia, Inc. | High-throughput serotyping and antibody profiling assays |
| US20240174992A1 (en) | 2022-05-04 | 2024-05-30 | Promega Corporation | Split modified dehalogenase variants |
| JP2025515181A (ja) * | 2022-05-04 | 2025-05-13 | プロメガ コーポレイション | 伸長表面ループ領域を有する修飾デハロゲナーゼ |
| US20230357266A1 (en) | 2022-05-04 | 2023-11-09 | Promega Corporation | Photoactivatable compounds and uses thereof |
| JP2025521803A (ja) | 2022-07-01 | 2025-07-10 | ジーンクアンタム ヘルスケア （スーチョウ） シーオー．， エルティーディー． | 固定化エンドグリコシダーゼ融合タンパク質及びその使用 |
| US20240368565A1 (en) * | 2023-05-04 | 2024-11-07 | Promega Corporation | Complementation-based tags and reporters for dual-modality labeling |
| WO2025090949A1 (en) * | 2023-10-27 | 2025-05-01 | Trustees Of Tufts College | Fluorogenic self-labeling enzyme compositions, systems, and kits |
| CN118165960B (zh) * | 2024-03-25 | 2025-03-25 | 南京大学 | 一种内切β-1,4-葡聚糖酶突变体及其基因、载体和制备方法 |
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| DE1229532B (de) | 1961-04-14 | 1966-12-01 | Boehringer Sohn Ingelheim | Verfahren zur Herstellung neuer Piperidinderivate |
| US3420367A (en) | 1967-05-25 | 1969-01-07 | Du Pont | Multiple container package |
| US4574079A (en) | 1983-05-27 | 1986-03-04 | Gavras Haralambos P | Radiolabeled angiotensin converting enzyme inhibitors for radiolabeling mammalian organ sites |
| US4612302A (en) | 1983-11-14 | 1986-09-16 | Brigham And Women's Hospital | Clinical use of somatostatin analogues |
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| US4812409A (en) | 1986-01-31 | 1989-03-14 | Eastman Kodak Company | Hydrolyzable fluorescent substrates and analytical determinations using same |
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| US5099020A (en) | 1989-11-27 | 1992-03-24 | Abbott Laboratories | Barbiturate assay compositions and methods |
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| WO2004009788A2 (en) | 2002-07-19 | 2004-01-29 | Diversa Corporation | Fluorescent proteins, nucleic acids encoding them and methods for making and using them |
| EP1567862A4 (en) | 2002-11-22 | 2007-04-18 | Univ Carnegie Mellon | COMPOSITIONS AND METHODS FOR REVERSIBLE INFLAMMATION OF BIOMOLECULES |
| US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
| EP1594962B1 (en) | 2003-01-31 | 2014-08-27 | Promega Corporation | Covalent tethering of functional groups to proteins |
| US20050095651A1 (en) | 2003-08-12 | 2005-05-05 | The Regents Of The University Of California | Photoswitchable method for the ordered attachment of proteins to surfaces |
| US8293503B2 (en) | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
| US20070087400A1 (en) | 2004-07-30 | 2007-04-19 | Aldis Darzins | Covalent tethering of functional groups to proteins and substrates therefor |
| US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
| EP1989548A2 (en) | 2006-02-08 | 2008-11-12 | Promega Corporation | Compositions and methods for capturing and analyzing cross-linked biomolecules |
| CA2667697A1 (en) | 2006-10-30 | 2008-05-08 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and functional expression |
-
2007
- 2007-10-30 CA CA002667697A patent/CA2667697A1/en not_active Abandoned
- 2007-10-30 US US11/978,950 patent/US8420367B2/en active Active
- 2007-10-30 EP EP12153529.8A patent/EP2492342B1/en active Active
- 2007-10-30 EP EP11075056.9A patent/EP2374875B1/en active Active
- 2007-10-30 JP JP2009534717A patent/JP5680302B2/ja active Active
- 2007-10-30 EP EP12153531.4A patent/EP2502990B1/en active Active
- 2007-10-30 EP EP07867352A patent/EP2087107A2/en not_active Withdrawn
- 2007-10-30 WO PCT/US2007/023205 patent/WO2008054821A2/en not_active Ceased
-
2012
- 2012-12-03 JP JP2012264713A patent/JP5840117B2/ja active Active
-
2013
- 2013-04-16 US US13/863,924 patent/US8748148B2/en active Active
-
2014
- 2014-05-22 US US14/285,327 patent/US9593316B2/en active Active
-
2017
- 2017-01-24 US US15/414,291 patent/US9873866B2/en active Active
-
2018
- 2018-01-23 US US15/877,980 patent/US10246690B2/en active Active
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