JP5680302B2 - キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質 - Google Patents

キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質 Download PDF

Info

Publication number
JP5680302B2
JP5680302B2 JP2009534717A JP2009534717A JP5680302B2 JP 5680302 B2 JP5680302 B2 JP 5680302B2 JP 2009534717 A JP2009534717 A JP 2009534717A JP 2009534717 A JP2009534717 A JP 2009534717A JP 5680302 B2 JP5680302 B2 JP 5680302B2
Authority
JP
Japan
Prior art keywords
protein
mutant
substrate
hydrolase
dehalogenase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2009534717A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010508023A (ja
Inventor
ダージンズ，アルディス
ピー． エンセル，ランス
ピー． エンセル，ランス
フリードマン オハナ，レイチェル
フリードマン オハナ，レイチェル
オットー，ポール
ビドゥギリス，ゲディミナス
ブイ． ウッド，キース
ブイ． ウッド，キース
ジー． ウッド，モニカ
ジー． ウッド，モニカ
ジマーマン，クリス
アール． スレイター，マイケル
アール． スレイター，マイケル
チン チャオ，ケイト
チン チャオ，ケイト
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of JP2010508023A publication Critical patent/JP2010508023A/ja
Application granted granted Critical
Publication of JP5680302B2 publication Critical patent/JP5680302B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y308/00—Hydrolases acting on halide bonds (3.8)
    • C12Y308/01—Hydrolases acting on halide bonds (3.8) in C-halide substances (3.8.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2009534717A 2006-10-30 2007-10-30 キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質 Active JP5680302B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US85523706P 2006-10-30 2006-10-30
US60/855,237 2006-10-30
US93020107P 2007-05-15 2007-05-15
US60/930,201 2007-05-15
PCT/US2007/023205 WO2008054821A2 (en) 2006-10-30 2007-10-30 Mutant hydrolase proteins with enhanced kinetics and functional expression

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2012264713A Division JP5840117B2 (ja) 2006-10-30 2012-12-03 キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質

Publications (2)

Publication Number Publication Date
JP2010508023A JP2010508023A (ja) 2010-03-18
JP5680302B2 true JP5680302B2 (ja) 2015-03-04

Family

ID=39344927

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2009534717A Active JP5680302B2 (ja) 2006-10-30 2007-10-30 キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質
JP2012264713A Active JP5840117B2 (ja) 2006-10-30 2012-12-03 キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2012264713A Active JP5840117B2 (ja) 2006-10-30 2012-12-03 キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質

Country Status (5)

Country Link
US (5) US8420367B2 (enExample)
EP (4) EP2492342B1 (enExample)
JP (2) JP5680302B2 (enExample)
CA (1) CA2667697A1 (enExample)
WO (1) WO2008054821A2 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
EP2635583B1 (en) 2010-11-02 2015-06-03 Promega Corporation Coelenterazine derivatives and methods of using same
AU2011323418B2 (en) 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use
JP6297559B2 (ja) 2012-07-31 2018-03-20 バジル・エム・ハンタッシュＢａｓｉｌ Ｍ． ＨＡＮＴＡＳＨ Ｈｌａｇ改変された細胞および方法
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
KR20190025362A (ko) * 2017-09-01 2019-03-11 삼성전자주식회사 할로산 데할로게나제 수퍼패밀리 단백질 변이체 및 그를 이용한 시료 중 불소 함유 화합물의 농도를 감소시키는 방법
IL280880B2 (en) 2018-08-27 2025-04-01 Regeneron Pharma Using Raman Spectroscopy in Downstream Purification
EP3932934A4 (en) * 2019-02-27 2023-09-20 National University Corporation Tokyo Medical and Dental University FUSION PROTEIN OF AN ANTIGEN BINDING PROTEIN AND FLUORESCENT PROTEIN OR FLUORESCENCE-LABLED TAG PROTEIN
CN110819612A (zh) * 2019-11-29 2020-02-21 天津科技大学 新型抗自切碱性蛋白酶的筛选
WO2022160156A1 (en) 2021-01-28 2022-08-04 Genequantum Healthcare (Suzhou) Co., Ltd. Ligase fusion proteins and applications thereof
JP2022127607A (ja) * 2021-02-19 2022-08-31 国立研究開発法人産業技術総合研究所 標的タンパク質の生産方法
CN115322980B (zh) * 2021-05-11 2024-04-02 中国科学院化学研究所 酰胺水解酶突变体及其在手性环状化合物合成上的应用
EP4206674B1 (en) 2021-12-28 2025-10-22 Encodia, Inc. High-throughput serotyping and antibody profiling assays
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
JP2025515181A (ja) * 2022-05-04 2025-05-13 プロメガ コーポレイション 伸長表面ループ領域を有する修飾デハロゲナーゼ
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
JP2025521803A (ja) 2022-07-01 2025-07-10 ジーンクアンタム ヘルスケア （スーチョウ） シーオー．， エルティーディー． 固定化エンドグリコシダーゼ融合タンパク質及びその使用
US20240368565A1 (en) * 2023-05-04 2024-11-07 Promega Corporation Complementation-based tags and reporters for dual-modality labeling
WO2025090949A1 (en) * 2023-10-27 2025-05-01 Trustees Of Tufts College Fluorogenic self-labeling enzyme compositions, systems, and kits
CN118165960B (zh) * 2024-03-25 2025-03-25 南京大学 一种内切β-1,4-葡聚糖酶突变体及其基因、载体和制备方法

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3131122A (en) 1961-04-14 1964-04-28 Boehringer Sohn Ingelheim Method of producing analgesia with n-substituted-4-phenyl-4-carbalkoxypiperidines
DE1229532B (de) 1961-04-14 1966-12-01 Boehringer Sohn Ingelheim Verfahren zur Herstellung neuer Piperidinderivate
US3420367A (en) 1967-05-25 1969-01-07 Du Pont Multiple container package
US4574079A (en) 1983-05-27 1986-03-04 Gavras Haralambos P Radiolabeled angiotensin converting enzyme inhibitors for radiolabeling mammalian organ sites
US4612302A (en) 1983-11-14 1986-09-16 Brigham And Women's Hospital Clinical use of somatostatin analogues
US4684620A (en) 1984-09-04 1987-08-04 Gibson-Stephens Neuropharmaceuticals, Inc. Cyclic polypeptides having mu-receptor specificity
US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
US4853371A (en) 1986-06-17 1989-08-01 The Administrators Of The Tulane Educational Fund Therapeutic somatostatin analogs
DE3778458D1 (de) 1986-09-04 1992-05-27 Idemitsu Kosan Co Fluessigkristallpolymere.
US4810636A (en) 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
CS259396B1 (sk) 1987-05-06 1988-10-14 Jozef Luston l,2,2,6,6-PentametyI-4-(w-brĎmalkoxy)piperidíny a spásob ich přípravy
SE8900130L (sv) 1989-01-16 1990-07-17 Klaus Mosbach Konceptet att med hjaelp av molekylavtrycksmetoden framstaella konstgjorda antikroppar genom imprinting av t ex antigener samt att framstaella konstgjorda entzymer genom imprintning med transition state analoger
US5071469A (en) 1989-04-21 1991-12-10 E. I. Du Pont De Nemours And Company Herbicidal benzylsulfonamides
US5498694A (en) 1989-05-25 1996-03-12 La Jolla Cancer Research Foundation Peptides of the cytoplasmic domain of integrin
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5099020A (en) 1989-11-27 1992-03-24 Abbott Laboratories Barbiturate assay compositions and methods
WO1992009690A2 (en) 1990-12-03 1992-06-11 Genentech, Inc. Enrichment method for variant proteins with altered binding properties
US5576424A (en) 1991-08-23 1996-11-19 Molecular Probes, Inc. Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells
FR2700855B1 (fr) 1993-01-28 1995-03-03 Commissariat Energie Atomique Dosage immunométrique d'un antigène ou d'un haptène.
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5372944A (en) 1993-10-14 1994-12-13 The Dow Chemical Company Method for conversion of halogenated hydrocarbons to halohydrins
US5523209A (en) 1994-03-14 1996-06-04 The Scripps Research Institute Methods for identifying inhibitors of integrin activation
US5503977A (en) 1994-04-22 1996-04-02 California Institute Of Technology Split ubiquitin protein sensor
JP3583489B2 (ja) 1994-12-22 2004-11-04 日清紡績株式会社 カルボジイミド誘導体
CH689633A5 (de) 1995-01-10 1999-07-30 Von Roll Umwelttechnik Ag Verfahren zur Kuehlung und Reinigung von Rauchgasen.
NZ322686A (en) 1995-10-19 2000-01-28 Univ Washington Discrete-length polyethene glycols
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5786428A (en) 1996-03-27 1998-07-28 California Institute Of Technology Adsorbents for amino acid and peptide separation
US6255461B1 (en) 1996-04-05 2001-07-03 Klaus Mosbach Artificial antibodies to corticosteroids prepared by molecular imprinting
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US6416733B1 (en) 1996-10-07 2002-07-09 Bristol-Myers Squibb Pharma Company Radiopharmaceuticals for imaging infection and inflammation
US5932421A (en) 1996-12-06 1999-08-03 The Scripps Research Institute Methods and cell lines for identification of regulators of integrin activation
IL131209A0 (en) 1997-02-13 2001-01-28 Dow Chemical Co Recombinant haloaliphatic dehalogenases
US6333154B1 (en) 1997-12-04 2001-12-25 Institut Pasteur Bacterial multi-hybrid system and applications thereof
CA2361927A1 (en) * 1999-03-09 2000-09-14 Diversa Corporation End selection in directed evolution
US20020042055A1 (en) 1999-12-23 2002-04-11 Affholter Joseph A. Alteration of hydrolase genes and screening of the resulting libraries for the ability to catalyze specific reactions
FR2804116B1 (fr) 2000-01-20 2002-08-23 Centre Nat Rech Scient Composes organosilicies, leur procede de preparation et leurs utilisations
AU2001238485A1 (en) 2000-02-18 2001-08-27 The Immune Response Corporation Methods and compositions for gene delivery
JP2003530365A (ja) 2000-04-10 2003-10-14 ザ スクリプス リサーチ インスティチュート プロテオミック分析
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP2004510767A (ja) 2000-10-02 2004-04-08 モレキュラー・プロウブズ・インコーポレーテッド アルデヒド部分またはケトン部分を有する生物分子を標識するための試薬
US20020137171A1 (en) 2000-12-01 2002-09-26 Diversa Corporation Hydrolase enzymes and their use in kinetic resolution
JP4589602B2 (ja) 2000-12-01 2010-12-01 ヴェレニウム コーポレイション デハロゲナーゼ活性を有する酵素およびその利用方法
US6800453B2 (en) 2001-01-23 2004-10-05 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
DE60237792D1 (de) 2001-04-10 2010-11-04 Ecole Polytech Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen
EA200400648A1 (ru) 2001-11-08 2005-04-28 Элан Фармасьютикалз, Инк. N, n'-замещенные производные 1,3-диамино-2-гидроксипропана
WO2004009788A2 (en) 2002-07-19 2004-01-29 Diversa Corporation Fluorescent proteins, nucleic acids encoding them and methods for making and using them
EP1567862A4 (en) 2002-11-22 2007-04-18 Univ Carnegie Mellon COMPOSITIONS AND METHODS FOR REVERSIBLE INFLAMMATION OF BIOMOLECULES
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US20050095651A1 (en) 2003-08-12 2005-05-05 The Regents Of The University Of California Photoswitchable method for the ordered attachment of proteins to surfaces
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
EP1989548A2 (en) 2006-02-08 2008-11-12 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression

Also Published As

Publication number Publication date
EP2374875B1 (en) 2015-09-02
EP2374875A2 (en) 2011-10-12
EP2502990A3 (en) 2013-04-17
EP2502990A2 (en) 2012-09-26
WO2008054821A3 (en) 2008-12-24
EP2492342B1 (en) 2015-06-03
US9593316B2 (en) 2017-03-14
EP2492342A1 (en) 2012-08-29
JP2010508023A (ja) 2010-03-18
JP2013078329A (ja) 2013-05-02
WO2008054821A2 (en) 2008-05-08
US20080145882A1 (en) 2008-06-19
EP2087107A2 (en) 2009-08-12
EP2374875A3 (en) 2012-10-10
US8420367B2 (en) 2013-04-16
US20150140636A1 (en) 2015-05-21
CA2667697A1 (en) 2008-05-08
US10246690B2 (en) 2019-04-02
US20180223267A1 (en) 2018-08-09
US8748148B2 (en) 2014-06-10
US9873866B2 (en) 2018-01-23
US20170137793A1 (en) 2017-05-18
US20130302880A1 (en) 2013-11-14
JP5840117B2 (ja) 2016-01-06
EP2502990B1 (en) 2015-07-22

Similar Documents

Publication Publication Date Title
JP5680302B2 (ja) キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質
JP4748685B2 (ja) タンパク質に対する官能基の共有結合的テザリング
US20090253131A1 (en) Hybrid fusion reporter and uses thereof
JP5214244B2 (ja) タンパク質およびその基質への、共有結合による機能性基の係留
US20100273186A1 (en) Split mutant hydrolase fusion reporter and uses thereof
JP5758768B2 (ja) タンパク質およびその基質への、共有結合による機能性基の係留
CN101595215A (zh) 具有增强的动力学和功能性表达的突变水解酶蛋白

Legal Events

Date Code Title Description
RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20110506

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20110506

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20111124

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20120223

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20120301

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120524

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20120802

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20121203

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130121

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20130124

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20130322

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141118

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150107

R150 Certificate of patent or registration of utility model

Ref document number: 5680302

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250