KR101168623B1 - 피롤로퀴놀린 퀴논 의존 가용성 글루코오스 탈수소효소의 개선된 돌연변이체 - Google Patents

피롤로퀴놀린 퀴논 의존 가용성 글루코오스 탈수소효소의 개선된 돌연변이체 Download PDF

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KR101168623B1
KR101168623B1 KR1020087025007A KR20087025007A KR101168623B1 KR 101168623 B1 KR101168623 B1 KR 101168623B1 KR 1020087025007 A KR1020087025007 A KR 1020087025007A KR 20087025007 A KR20087025007 A KR 20087025007A KR 101168623 B1 KR101168623 B1 KR 101168623B1
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gdh
glucose
mutant
amino acid
maltose
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KR20080106349A (ko
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마라 뵈니츠-두라트
다니엘라 베크
페터 크라츠슈
라이너 슈무크
데르 엘츠 헤르베르트 폰
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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KR1020087025007A 2006-04-13 2007-04-11 피롤로퀴놀린 퀴논 의존 가용성 글루코오스 탈수소효소의 개선된 돌연변이체 Active KR101168623B1 (ko)

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Application Number Priority Date Filing Date Title
EP06007779.9 2006-04-13
EP06007779 2006-04-13
PCT/EP2007/003207 WO2007118647A1 (en) 2006-04-13 2007-04-11 Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase

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KR20080106349A KR20080106349A (ko) 2008-12-04
KR101168623B1 true KR101168623B1 (ko) 2012-08-03

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US (1) US7732179B2 (enExample)
EP (2) EP2010649B1 (enExample)
JP (3) JP5683809B2 (enExample)
KR (1) KR101168623B1 (enExample)
CN (1) CN101421396B (enExample)
AU (1) AU2007237478B2 (enExample)
BR (1) BRPI0710131B1 (enExample)
CA (1) CA2649135C (enExample)
ES (2) ES2624805T3 (enExample)
HU (2) HUE049911T2 (enExample)
MX (1) MX2008013001A (enExample)
PL (2) PL3246402T3 (enExample)
PT (2) PT2010649T (enExample)
WO (1) WO2007118647A1 (enExample)

Families Citing this family (16)

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Publication number Priority date Publication date Assignee Title
JP5753720B2 (ja) * 2010-04-22 2015-07-22 アークレイ株式会社 バイオセンサ
US20130092536A1 (en) 2010-07-23 2013-04-18 Roche Diagnostics Operations, Inc. Zwitterion buffer containing compositions and uses in electroanalytical methods
US20130337485A1 (en) 2010-12-20 2013-12-19 Lek Pharmaceuticals D.D Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
EP3901624B1 (en) 2010-12-22 2024-01-31 Roche Diabetes Care GmbH Methods to compensate for sources of error during electrochemical testing
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
CA2865678C (en) 2012-04-19 2018-03-13 Susanne BALDUS Method and device for determining an analyte concentration in blood
EP2811299A1 (en) 2013-06-07 2014-12-10 Roche Diagniostics GmbH Test element for detecting at least one analyte in a body fluid
JP6998863B2 (ja) 2015-09-25 2022-02-04 エフ．ホフマン－ラ ロシュ アーゲー 深共融溶媒におけるソルターゼａを利用したアミド基転移
WO2017050874A1 (en) 2015-09-25 2017-03-30 F. Hoffmann-La Roche Ag Process for producing thioesters employing a sortase a
CN105331590B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
CN111073866B (zh) 2015-10-29 2024-03-19 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及其在葡萄糖检测中的应用
CN108350439A (zh) 2015-12-21 2018-07-31 豪夫迈·罗氏有限公司 来自粪产碱菌的突变体3-羟基丁酸脱氢酶及其相关方法和用途
WO2017137491A1 (en) 2016-02-09 2017-08-17 Roche Diabetes Care Gmbh Mutant 3-hydroxybutyrate dehydrogenase from rhodobacter sphaeroides as well as methods and uses involving the same
PL3523639T3 (pl) 2016-10-05 2025-03-17 F. Hoffmann-La Roche Ag Odczynniki wykrywające i układy elektrod do elementów diagnostycznych testów wieloanalitowych oraz sposoby ich zastosowania
CN115820608B (zh) * 2022-08-31 2024-06-04 中国海洋大学 λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002034919A1 (en) 2000-10-27 2002-05-02 Roche Diagnostics Gmbh Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
WO2006008132A1 (en) 2004-07-20 2006-01-26 Roche Diagnostics Gmbh Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
CA2368461A1 (en) * 1999-02-19 2000-08-24 Winfried Linxweiler Glucose dehydrogenase fusion proteins and their utilization in expression systems
JP2000350588A (ja) * 1999-04-08 2000-12-19 Koji Hayade グルコース脱水素酵素
TWI224136B (en) 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
JP2004344145A (ja) * 2002-05-27 2004-12-09 Toyobo Co Ltd 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
JP2004173538A (ja) * 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002034919A1 (en) 2000-10-27 2002-05-02 Roche Diagnostics Gmbh Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
US20030104595A1 (en) 2000-10-27 2003-06-05 Peter Kratzch Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
WO2006008132A1 (en) 2004-07-20 2006-01-26 Roche Diagnostics Gmbh Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

Also Published As

Publication number Publication date
EP3246402A1 (en) 2017-11-22
PL3246402T3 (pl) 2020-10-19
BRPI0710131B1 (pt) 2020-03-10
EP3246402B1 (en) 2020-05-20
AU2007237478B2 (en) 2011-05-19
US7732179B2 (en) 2010-06-08
PL2010649T3 (pl) 2017-08-31
PT2010649T (pt) 2017-05-03
HUE049911T2 (hu) 2020-11-30
ES2807530T3 (es) 2021-02-23
PT3246402T (pt) 2020-07-14
ES2624805T3 (es) 2017-07-17
HUE032804T2 (en) 2017-11-28
CA2649135A1 (en) 2007-10-25
MX2008013001A (es) 2008-10-17
EP2010649A1 (en) 2009-01-07
AU2007237478A1 (en) 2007-10-25
JP2016119904A (ja) 2016-07-07
HK1131633A1 (zh) 2010-01-29
US20090148874A1 (en) 2009-06-11
JP5683809B2 (ja) 2015-03-11
JP6185617B2 (ja) 2017-08-23
JP2009533033A (ja) 2009-09-17
CA2649135C (en) 2013-11-19
CN101421396B (zh) 2016-01-20
CN101421396A (zh) 2009-04-29
JP2013198497A (ja) 2013-10-03
JP5937547B2 (ja) 2016-06-22
KR20080106349A (ko) 2008-12-04
EP2010649B1 (en) 2017-03-08
BRPI0710131A2 (pt) 2011-08-02
WO2007118647A1 (en) 2007-10-25

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