AU2007237478B2 - Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase - Google Patents

Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase Download PDF

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Publication number
AU2007237478B2
AU2007237478B2 AU2007237478A AU2007237478A AU2007237478B2 AU 2007237478 B2 AU2007237478 B2 AU 2007237478B2 AU 2007237478 A AU2007237478 A AU 2007237478A AU 2007237478 A AU2007237478 A AU 2007237478A AU 2007237478 B2 AU2007237478 B2 AU 2007237478B2
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Prior art keywords
mutant
gdh
glucose
amino acid
maltose
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AU2007237478A1 (en
Inventor
Daniela Beck
Mara Boenitz-Dulat
Peter Kratzsch
Rainer Schmuck
Herbert Von Der Eltz
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2007237478A 2006-04-13 2007-04-11 Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase Active AU2007237478B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06007779 2006-04-13
EP06007779.9 2006-04-13
PCT/EP2007/003207 WO2007118647A1 (en) 2006-04-13 2007-04-11 Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase

Publications (2)

Publication Number Publication Date
AU2007237478A1 AU2007237478A1 (en) 2007-10-25
AU2007237478B2 true AU2007237478B2 (en) 2011-05-19

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Family Applications (1)

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AU2007237478A Active AU2007237478B2 (en) 2006-04-13 2007-04-11 Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase

Country Status (14)

Country Link
US (1) US7732179B2 (enExample)
EP (2) EP2010649B1 (enExample)
JP (3) JP5683809B2 (enExample)
KR (1) KR101168623B1 (enExample)
CN (1) CN101421396B (enExample)
AU (1) AU2007237478B2 (enExample)
BR (1) BRPI0710131B1 (enExample)
CA (1) CA2649135C (enExample)
ES (2) ES2807530T3 (enExample)
HU (2) HUE032804T2 (enExample)
MX (1) MX2008013001A (enExample)
PL (2) PL2010649T3 (enExample)
PT (2) PT2010649T (enExample)
WO (1) WO2007118647A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5753720B2 (ja) * 2010-04-22 2015-07-22 アークレイ株式会社 バイオセンサ
US20130092536A1 (en) 2010-07-23 2013-04-18 Roche Diagnostics Operations, Inc. Zwitterion buffer containing compositions and uses in electroanalytical methods
CA2822331A1 (en) 2010-12-20 2012-07-19 Lek Pharmaceuticals D.D. Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
EP3901624B1 (en) 2010-12-22 2024-01-31 Roche Diabetes Care GmbH Methods to compensate for sources of error during electrochemical testing
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
WO2013156526A1 (de) 2012-04-19 2013-10-24 F. Hoffmann-La Roche Ag Verfahren und vorrichtung zur bestimmung einer analytkonzentration in blut
EP2811299A1 (en) 2013-06-07 2014-12-10 Roche Diagniostics GmbH Test element for detecting at least one analyte in a body fluid
EP3353314A1 (en) 2015-09-25 2018-08-01 H. Hoffnabb-La Roche Ag Transamidation employing sortase a in deep eutectic solvents
JP6895953B2 (ja) 2015-09-25 2021-06-30 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ソルターゼａを利用してチオエステルを作製するための方法
CN105331590B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
CN105331591B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及葡萄糖检测装置
BR112018012665A2 (pt) 2015-12-21 2018-12-04 Hoffmann La Roche 3-hidroxibutirato desidrogenase mutante de alcaligenes faecalis, ácido nucleico, célula, método para determinar a quantidade ou concentração de 3-hidroxibutirato, uso da 3-hbdh mutante e dispositivo
WO2017137491A1 (en) 2016-02-09 2017-08-17 Roche Diabetes Care Gmbh Mutant 3-hydroxybutyrate dehydrogenase from rhodobacter sphaeroides as well as methods and uses involving the same
EP4481375A3 (en) 2016-10-05 2025-04-02 F. Hoffmann-La Roche AG Detection reagents and electrode arrangements for multi-analyte diagnostic test elements, as well as methods of using the same
CN115820608B (zh) * 2022-08-31 2024-06-04 中国海洋大学 λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
KR20010103017A (ko) * 1999-02-19 2001-11-17 플레믹 크리스티안 글루코스 데히드로게나제 융합 단백질 및 발현계에서의그의 용도
JP2000350588A (ja) * 1999-04-08 2000-12-19 Koji Hayade グルコース脱水素酵素
TWI224136B (en) 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
EP1332216B1 (en) * 2000-10-27 2011-08-17 Roche Diagnostics GmbH Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
JP2004344145A (ja) * 2002-05-27 2004-12-09 Toyobo Co Ltd 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素
BRPI0513691A (pt) * 2004-07-20 2008-03-25 Roche Diagnostics Gmbh glicose desidrogenase dependente de pirroloquinolina quinona transformada geneticamente compreendendo uma inserção de aminoácido

Also Published As

Publication number Publication date
JP2016119904A (ja) 2016-07-07
US7732179B2 (en) 2010-06-08
MX2008013001A (es) 2008-10-17
JP2013198497A (ja) 2013-10-03
WO2007118647A1 (en) 2007-10-25
BRPI0710131A2 (pt) 2011-08-02
HUE049911T2 (hu) 2020-11-30
EP2010649B1 (en) 2017-03-08
JP6185617B2 (ja) 2017-08-23
KR101168623B1 (ko) 2012-08-03
CA2649135C (en) 2013-11-19
AU2007237478A1 (en) 2007-10-25
PT3246402T (pt) 2020-07-14
PL3246402T3 (pl) 2020-10-19
JP2009533033A (ja) 2009-09-17
CN101421396B (zh) 2016-01-20
HUE032804T2 (en) 2017-11-28
US20090148874A1 (en) 2009-06-11
PL2010649T3 (pl) 2017-08-31
CN101421396A (zh) 2009-04-29
BRPI0710131B1 (pt) 2020-03-10
EP2010649A1 (en) 2009-01-07
ES2624805T3 (es) 2017-07-17
HK1131633A1 (zh) 2010-01-29
EP3246402A1 (en) 2017-11-22
ES2807530T3 (es) 2021-02-23
EP3246402B1 (en) 2020-05-20
JP5937547B2 (ja) 2016-06-22
CA2649135A1 (en) 2007-10-25
PT2010649T (pt) 2017-05-03
KR20080106349A (ko) 2008-12-04
JP5683809B2 (ja) 2015-03-11

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