CN101421396B - 吡咯并喹啉醌依赖性可溶性葡萄糖脱氢酶的改良突变体 - Google Patents

吡咯并喹啉醌依赖性可溶性葡萄糖脱氢酶的改良突变体 Download PDF

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CN101421396B
CN101421396B CN200780013259.8A CN200780013259A CN101421396B CN 101421396 B CN101421396 B CN 101421396B CN 200780013259 A CN200780013259 A CN 200780013259A CN 101421396 B CN101421396 B CN 101421396B
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mutant
gdh
glucose
amino acid
mutants
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CN101421396A (zh
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M·博尼茨-多莱特
D·贝克
P·克拉茨什
R·施穆克
H·冯德埃尔茨
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN200780013259.8A 2006-04-13 2007-04-11 吡咯并喹啉醌依赖性可溶性葡萄糖脱氢酶的改良突变体 Active CN101421396B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06007779.9 2006-04-13
EP06007779 2006-04-13
PCT/EP2007/003207 WO2007118647A1 (en) 2006-04-13 2007-04-11 Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase

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CN101421396A CN101421396A (zh) 2009-04-29
CN101421396B true CN101421396B (zh) 2016-01-20

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US (1) US7732179B2 (enExample)
EP (2) EP3246402B1 (enExample)
JP (3) JP5683809B2 (enExample)
KR (1) KR101168623B1 (enExample)
CN (1) CN101421396B (enExample)
AU (1) AU2007237478B2 (enExample)
BR (1) BRPI0710131B1 (enExample)
CA (1) CA2649135C (enExample)
ES (2) ES2624805T3 (enExample)
HU (2) HUE032804T2 (enExample)
MX (1) MX2008013001A (enExample)
PL (2) PL2010649T3 (enExample)
PT (2) PT3246402T (enExample)
WO (1) WO2007118647A1 (enExample)

Families Citing this family (16)

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JP5753720B2 (ja) * 2010-04-22 2015-07-22 アークレイ株式会社 バイオセンサ
WO2012010308A1 (en) 2010-07-23 2012-01-26 Roche Diagnostics Gmbh Zwitterion buffer containing compositions and uses in electroanalytical devices and methods
EP2655650B1 (en) 2010-12-20 2017-12-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
EP2656058A1 (en) 2010-12-22 2013-10-30 Roche Diagnostics GmbH Systems and methods to compensate for sources of error during electrochemical testing
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
EP2839267B1 (de) 2012-04-19 2019-12-04 F.Hoffmann-La Roche Ag Verfahren und vorrichtung zur bestimmung einer analytkonzentration in blut
EP2811299A1 (en) 2013-06-07 2014-12-10 Roche Diagniostics GmbH Test element for detecting at least one analyte in a body fluid
EP3353310B1 (en) 2015-09-25 2020-04-29 H. Hoffnabb-La Roche Ag Process for producing thioesters employing a sortase a
WO2017050872A1 (en) 2015-09-25 2017-03-30 F. Hoffmann-La Roche Ag Transamidation employing sortase a in deep eutectic solvents
CN111073866B (zh) 2015-10-29 2024-03-19 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及其在葡萄糖检测中的应用
CN105331590B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
WO2017109003A1 (en) 2015-12-21 2017-06-29 F. Hoffmann-La Roche Ag Mutant 3-hydroxybutyrate dehydrogenase from alcaligenes faecalis as well as methods and uses involving the same
ES2782199T3 (es) 2016-02-09 2020-09-11 Hoffmann La Roche 3-Hidroxibutirato deshidrogenasa mutante de Rhodobacter sphaeroides, así como procedimientos y usos que implican la misma
PL3523639T3 (pl) 2016-10-05 2025-03-17 F. Hoffmann-La Roche Ag Odczynniki wykrywające i układy elektrod do elementów diagnostycznych testów wieloanalitowych oraz sposoby ich zastosowania
CN115820608B (zh) * 2022-08-31 2024-06-04 中国海洋大学 λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1340104A (zh) * 1999-02-19 2002-03-13 默克专利股份有限公司 葡萄糖脱氢酶融合蛋白及其在表达系统中的用途
CN1350579A (zh) * 1999-04-08 2002-05-22 早出广司 葡萄糖脱氢酶
US20030104595A1 (en) * 2000-10-27 2003-06-05 Peter Kratzch Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

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DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
TWI224136B (en) 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
JP2004344145A (ja) * 2002-05-27 2004-12-09 Toyobo Co Ltd 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素
WO2006008132A1 (en) 2004-07-20 2006-01-26 Roche Diagnostics Gmbh Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1340104A (zh) * 1999-02-19 2002-03-13 默克专利股份有限公司 葡萄糖脱氢酶融合蛋白及其在表达系统中的用途
CN1350579A (zh) * 1999-04-08 2002-05-22 早出广司 葡萄糖脱氢酶
US20030104595A1 (en) * 2000-10-27 2003-06-05 Peter Kratzch Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

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Publication number Publication date
HUE032804T2 (en) 2017-11-28
KR101168623B1 (ko) 2012-08-03
ES2807530T3 (es) 2021-02-23
US20090148874A1 (en) 2009-06-11
EP3246402A1 (en) 2017-11-22
WO2007118647A1 (en) 2007-10-25
BRPI0710131B1 (pt) 2020-03-10
CA2649135A1 (en) 2007-10-25
JP5683809B2 (ja) 2015-03-11
JP6185617B2 (ja) 2017-08-23
PL2010649T3 (pl) 2017-08-31
HK1131633A1 (zh) 2010-01-29
BRPI0710131A2 (pt) 2011-08-02
ES2624805T3 (es) 2017-07-17
US7732179B2 (en) 2010-06-08
JP2013198497A (ja) 2013-10-03
PT2010649T (pt) 2017-05-03
AU2007237478B2 (en) 2011-05-19
KR20080106349A (ko) 2008-12-04
CN101421396A (zh) 2009-04-29
EP3246402B1 (en) 2020-05-20
CA2649135C (en) 2013-11-19
JP2009533033A (ja) 2009-09-17
AU2007237478A1 (en) 2007-10-25
EP2010649B1 (en) 2017-03-08
PT3246402T (pt) 2020-07-14
MX2008013001A (es) 2008-10-17
HUE049911T2 (hu) 2020-11-30
PL3246402T3 (pl) 2020-10-19
JP5937547B2 (ja) 2016-06-22
JP2016119904A (ja) 2016-07-07
EP2010649A1 (en) 2009-01-07

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