ES2624805T3 - Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona - Google Patents
Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona Download PDFInfo
- Publication number
- ES2624805T3 ES2624805T3 ES07724148.7T ES07724148T ES2624805T3 ES 2624805 T3 ES2624805 T3 ES 2624805T3 ES 07724148 T ES07724148 T ES 07724148T ES 2624805 T3 ES2624805 T3 ES 2624805T3
- Authority
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- Prior art keywords
- mutant
- glucose
- gdh
- amino acid
- maltose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000001419 dependent effect Effects 0.000 title abstract description 8
- 108040005045 glucose dehydrogenase activity proteins Proteins 0.000 title description 2
- MMXZSJMASHPLLR-UHFFFAOYSA-N pyrroloquinoline quinone Chemical compound C12=C(C(O)=O)C=C(C(O)=O)N=C2C(=O)C(=O)C2=C1NC(C(=O)O)=C2 MMXZSJMASHPLLR-UHFFFAOYSA-N 0.000 title 2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06007779 | 2006-04-13 | ||
| EP06007779 | 2006-04-13 | ||
| PCT/EP2007/003207 WO2007118647A1 (en) | 2006-04-13 | 2007-04-11 | Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2624805T3 true ES2624805T3 (es) | 2017-07-17 |
Family
ID=36579921
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07724148.7T Active ES2624805T3 (es) | 2006-04-13 | 2007-04-11 | Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona |
| ES17151869T Active ES2807530T3 (es) | 2006-04-13 | 2007-04-11 | Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES17151869T Active ES2807530T3 (es) | 2006-04-13 | 2007-04-11 | Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US7732179B2 (enExample) |
| EP (2) | EP3246402B1 (enExample) |
| JP (3) | JP5683809B2 (enExample) |
| KR (1) | KR101168623B1 (enExample) |
| CN (1) | CN101421396B (enExample) |
| AU (1) | AU2007237478B2 (enExample) |
| BR (1) | BRPI0710131B1 (enExample) |
| CA (1) | CA2649135C (enExample) |
| ES (2) | ES2624805T3 (enExample) |
| HU (2) | HUE032804T2 (enExample) |
| MX (1) | MX2008013001A (enExample) |
| PL (2) | PL2010649T3 (enExample) |
| PT (2) | PT3246402T (enExample) |
| WO (1) | WO2007118647A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5753720B2 (ja) * | 2010-04-22 | 2015-07-22 | アークレイ株式会社 | バイオセンサ |
| WO2012010308A1 (en) | 2010-07-23 | 2012-01-26 | Roche Diagnostics Gmbh | Zwitterion buffer containing compositions and uses in electroanalytical devices and methods |
| EP2655650B1 (en) | 2010-12-20 | 2017-12-20 | LEK Pharmaceuticals d.d. | Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof |
| EP2465936A1 (en) | 2010-12-20 | 2012-06-20 | LEK Pharmaceuticals d.d. | Enzymatic synthesis of statins and intermediates thereof |
| EP2656058A1 (en) | 2010-12-22 | 2013-10-30 | Roche Diagnostics GmbH | Systems and methods to compensate for sources of error during electrochemical testing |
| CN102559624B (zh) * | 2012-03-05 | 2013-10-16 | 浙江德清汇宁生物科技有限公司 | 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法 |
| EP2839267B1 (de) | 2012-04-19 | 2019-12-04 | F.Hoffmann-La Roche Ag | Verfahren und vorrichtung zur bestimmung einer analytkonzentration in blut |
| EP2811299A1 (en) | 2013-06-07 | 2014-12-10 | Roche Diagniostics GmbH | Test element for detecting at least one analyte in a body fluid |
| EP3353310B1 (en) | 2015-09-25 | 2020-04-29 | H. Hoffnabb-La Roche Ag | Process for producing thioesters employing a sortase a |
| WO2017050872A1 (en) | 2015-09-25 | 2017-03-30 | F. Hoffmann-La Roche Ag | Transamidation employing sortase a in deep eutectic solvents |
| CN111073866B (zh) | 2015-10-29 | 2024-03-19 | 英科隆生物技术（杭州）有限公司 | PQQ-sGDH突变体、聚核苷酸及其在葡萄糖检测中的应用 |
| CN105331590B (zh) * | 2015-10-29 | 2020-02-28 | 英科隆生物技术（杭州）有限公司 | 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用 |
| WO2017109003A1 (en) | 2015-12-21 | 2017-06-29 | F. Hoffmann-La Roche Ag | Mutant 3-hydroxybutyrate dehydrogenase from alcaligenes faecalis as well as methods and uses involving the same |
| ES2782199T3 (es) | 2016-02-09 | 2020-09-11 | Hoffmann La Roche | 3-Hidroxibutirato deshidrogenasa mutante de Rhodobacter sphaeroides, así como procedimientos y usos que implican la misma |
| PL3523639T3 (pl) | 2016-10-05 | 2025-03-17 | F. Hoffmann-La Roche Ag | Odczynniki wykrywające i układy elektrod do elementów diagnostycznych testów wieloanalitowych oraz sposoby ich zastosowania |
| CN115820608B (zh) * | 2022-08-31 | 2024-06-04 | 中国海洋大学 | λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3716957A1 (de) | 1987-05-20 | 1988-12-01 | Boehringer Mannheim Gmbh | Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten |
| DE4311464A1 (de) | 1993-04-08 | 1994-10-13 | Boehringer Mannheim Gmbh | Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase |
| DE19639169A1 (de) | 1996-09-24 | 1998-04-02 | Boehringer Mannheim Gmbh | Redoxaktive Verbindungen und deren Anwendung |
| JPH10243786A (ja) | 1997-03-03 | 1998-09-14 | Koji Hayade | 改変型グルコース脱水素酵素 |
| US5997817A (en) | 1997-12-05 | 1999-12-07 | Roche Diagnostics Corporation | Electrochemical biosensor test strip |
| JPH11243949A (ja) | 1998-03-03 | 1999-09-14 | Toyobo Co Ltd | Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法 |
| WO2000049039A2 (de) * | 1999-02-19 | 2000-08-24 | Merck Patent Gmbh | Glucose-dehydrogenase-fusionsproteine und ihre verwendung in expressionssystemen |
| JP2000350588A (ja) * | 1999-04-08 | 2000-12-19 | Koji Hayade | グルコース脱水素酵素 |
| TWI224136B (en) | 1999-04-30 | 2004-11-21 | Koji Sode | Glucose dehydrogenase |
| WO2002034919A1 (en) * | 2000-10-27 | 2002-05-02 | Roche Diagnostics Gmbh | Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase |
| JP2004344145A (ja) * | 2002-05-27 | 2004-12-09 | Toyobo Co Ltd | 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体 |
| JP2004173538A (ja) | 2002-11-25 | 2004-06-24 | Amano Enzyme Inc | ピロロキノリンキノン依存性グルコース脱水素酵素 |
| WO2006008132A1 (en) | 2004-07-20 | 2006-01-26 | Roche Diagnostics Gmbh | Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion |
-
2007
- 2007-04-11 CA CA2649135A patent/CA2649135C/en active Active
- 2007-04-11 PL PL07724148T patent/PL2010649T3/pl unknown
- 2007-04-11 AU AU2007237478A patent/AU2007237478B2/en active Active
- 2007-04-11 ES ES07724148.7T patent/ES2624805T3/es active Active
- 2007-04-11 PT PT171518699T patent/PT3246402T/pt unknown
- 2007-04-11 WO PCT/EP2007/003207 patent/WO2007118647A1/en not_active Ceased
- 2007-04-11 HU HUE07724148A patent/HUE032804T2/en unknown
- 2007-04-11 MX MX2008013001A patent/MX2008013001A/es active IP Right Grant
- 2007-04-11 PL PL17151869T patent/PL3246402T3/pl unknown
- 2007-04-11 JP JP2009504634A patent/JP5683809B2/ja active Active
- 2007-04-11 CN CN200780013259.8A patent/CN101421396B/zh active Active
- 2007-04-11 KR KR1020087025007A patent/KR101168623B1/ko active Active
- 2007-04-11 EP EP17151869.9A patent/EP3246402B1/en active Active
- 2007-04-11 BR BRPI0710131-7A patent/BRPI0710131B1/pt active Search and Examination
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- 2007-04-11 EP EP07724148.7A patent/EP2010649B1/en active Active
- 2007-04-11 HU HUE17151869A patent/HUE049911T2/hu unknown
- 2007-04-11 PT PT77241487T patent/PT2010649T/pt unknown
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2008
- 2008-10-13 US US12/250,241 patent/US7732179B2/en active Active
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- 2013-06-21 JP JP2013130565A patent/JP5937547B2/ja active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| HUE032804T2 (en) | 2017-11-28 |
| KR101168623B1 (ko) | 2012-08-03 |
| ES2807530T3 (es) | 2021-02-23 |
| US20090148874A1 (en) | 2009-06-11 |
| EP3246402A1 (en) | 2017-11-22 |
| WO2007118647A1 (en) | 2007-10-25 |
| BRPI0710131B1 (pt) | 2020-03-10 |
| CA2649135A1 (en) | 2007-10-25 |
| JP5683809B2 (ja) | 2015-03-11 |
| JP6185617B2 (ja) | 2017-08-23 |
| PL2010649T3 (pl) | 2017-08-31 |
| HK1131633A1 (zh) | 2010-01-29 |
| BRPI0710131A2 (pt) | 2011-08-02 |
| US7732179B2 (en) | 2010-06-08 |
| JP2013198497A (ja) | 2013-10-03 |
| CN101421396B (zh) | 2016-01-20 |
| PT2010649T (pt) | 2017-05-03 |
| AU2007237478B2 (en) | 2011-05-19 |
| KR20080106349A (ko) | 2008-12-04 |
| CN101421396A (zh) | 2009-04-29 |
| EP3246402B1 (en) | 2020-05-20 |
| CA2649135C (en) | 2013-11-19 |
| JP2009533033A (ja) | 2009-09-17 |
| AU2007237478A1 (en) | 2007-10-25 |
| EP2010649B1 (en) | 2017-03-08 |
| PT3246402T (pt) | 2020-07-14 |
| MX2008013001A (es) | 2008-10-17 |
| HUE049911T2 (hu) | 2020-11-30 |
| PL3246402T3 (pl) | 2020-10-19 |
| JP5937547B2 (ja) | 2016-06-22 |
| JP2016119904A (ja) | 2016-07-07 |
| EP2010649A1 (en) | 2009-01-07 |
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