ES2624805T3 - Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona - Google Patents

Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona Download PDF

Info

Publication number
ES2624805T3
ES2624805T3 ES07724148.7T ES07724148T ES2624805T3 ES 2624805 T3 ES2624805 T3 ES 2624805T3 ES 07724148 T ES07724148 T ES 07724148T ES 2624805 T3 ES2624805 T3 ES 2624805T3
Authority
ES
Spain
Prior art keywords
mutant
glucose
gdh
amino acid
maltose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES07724148.7T
Other languages
English (en)
Spanish (es)
Inventor
Mara Boenitz-Dulat
Daniela Beck
Peter Kratzsch
Rainer Schmuck
Herbert Von Der Eltz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36579921&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2624805(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2624805T3 publication Critical patent/ES2624805T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
ES07724148.7T 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona Active ES2624805T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06007779 2006-04-13
EP06007779 2006-04-13
PCT/EP2007/003207 WO2007118647A1 (en) 2006-04-13 2007-04-11 Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase

Publications (1)

Publication Number Publication Date
ES2624805T3 true ES2624805T3 (es) 2017-07-17

Family

ID=36579921

Family Applications (2)

Application Number Title Priority Date Filing Date
ES07724148.7T Active ES2624805T3 (es) 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona
ES17151869T Active ES2807530T3 (es) 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES17151869T Active ES2807530T3 (es) 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona

Country Status (14)

Country Link
US (1) US7732179B2 (enExample)
EP (2) EP2010649B1 (enExample)
JP (3) JP5683809B2 (enExample)
KR (1) KR101168623B1 (enExample)
CN (1) CN101421396B (enExample)
AU (1) AU2007237478B2 (enExample)
BR (1) BRPI0710131B1 (enExample)
CA (1) CA2649135C (enExample)
ES (2) ES2624805T3 (enExample)
HU (2) HUE032804T2 (enExample)
MX (1) MX2008013001A (enExample)
PL (2) PL3246402T3 (enExample)
PT (2) PT2010649T (enExample)
WO (1) WO2007118647A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5753720B2 (ja) * 2010-04-22 2015-07-22 アークレイ株式会社 バイオセンサ
US20130092536A1 (en) 2010-07-23 2013-04-18 Roche Diagnostics Operations, Inc. Zwitterion buffer containing compositions and uses in electroanalytical methods
JP6194251B2 (ja) 2010-12-20 2017-09-06 レツク・フアーマシユーテイカルズ・デー・デー 医薬品有効成分およびこれの中間体の酵素的合成
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
EP2656058A1 (en) 2010-12-22 2013-10-30 Roche Diagnostics GmbH Systems and methods to compensate for sources of error during electrochemical testing
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
JP6111323B2 (ja) 2012-04-19 2017-04-05 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 血液中の検体濃度を測定する方法および装置
EP2811299A1 (en) 2013-06-07 2014-12-10 Roche Diagniostics GmbH Test element for detecting at least one analyte in a body fluid
EP3353310B1 (en) 2015-09-25 2020-04-29 H. Hoffnabb-La Roche Ag Process for producing thioesters employing a sortase a
JP6998863B2 (ja) 2015-09-25 2022-02-04 エフ．ホフマン－ラ ロシュ アーゲー 深共融溶媒におけるソルターゼａを利用したアミド基転移
CN105331591B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及葡萄糖检测装置
CN105331590B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
WO2017109003A1 (en) 2015-12-21 2017-06-29 F. Hoffmann-La Roche Ag Mutant 3-hydroxybutyrate dehydrogenase from alcaligenes faecalis as well as methods and uses involving the same
WO2017137491A1 (en) 2016-02-09 2017-08-17 Roche Diabetes Care Gmbh Mutant 3-hydroxybutyrate dehydrogenase from rhodobacter sphaeroides as well as methods and uses involving the same
JP2019529935A (ja) 2016-10-05 2019-10-17 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 多検体診断用試験エレメントのための検出試薬および電極配置、ならびにそれらを使用する方法
CN115820608B (zh) * 2022-08-31 2024-06-04 中国海洋大学 λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
BR0008370A (pt) * 1999-02-19 2001-11-06 Merck Patent Gmbh Proteìnas de fusão de glicose deshidrogenase e uso das mesmas em sistemas de expressão
JP2000350588A (ja) * 1999-04-08 2000-12-19 Koji Hayade グルコース脱水素酵素
TWI224136B (en) 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
AU1596602A (en) * 2000-10-27 2002-05-06 Peter Kratzsch Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
JP2004344145A (ja) * 2002-05-27 2004-12-09 Toyobo Co Ltd 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素
JP4842938B2 (ja) * 2004-07-20 2011-12-21 エフ．ホフマン−ラ ロシュ アーゲー アミノ酸挿入を含む遺伝子操作されたピロロキノリンキノン依存性グルコースデヒドロゲナーゼ

Also Published As

Publication number Publication date
ES2807530T3 (es) 2021-02-23
HK1131633A1 (zh) 2010-01-29
HUE032804T2 (en) 2017-11-28
JP2016119904A (ja) 2016-07-07
PT2010649T (pt) 2017-05-03
JP5683809B2 (ja) 2015-03-11
KR20080106349A (ko) 2008-12-04
MX2008013001A (es) 2008-10-17
EP3246402B1 (en) 2020-05-20
US20090148874A1 (en) 2009-06-11
AU2007237478A1 (en) 2007-10-25
CA2649135A1 (en) 2007-10-25
US7732179B2 (en) 2010-06-08
PT3246402T (pt) 2020-07-14
EP2010649A1 (en) 2009-01-07
EP3246402A1 (en) 2017-11-22
BRPI0710131B1 (pt) 2020-03-10
CA2649135C (en) 2013-11-19
PL2010649T3 (pl) 2017-08-31
BRPI0710131A2 (pt) 2011-08-02
HUE049911T2 (hu) 2020-11-30
JP6185617B2 (ja) 2017-08-23
WO2007118647A1 (en) 2007-10-25
JP2009533033A (ja) 2009-09-17
EP2010649B1 (en) 2017-03-08
CN101421396B (zh) 2016-01-20
PL3246402T3 (pl) 2020-10-19
AU2007237478B2 (en) 2011-05-19
JP2013198497A (ja) 2013-10-03
KR101168623B1 (ko) 2012-08-03
JP5937547B2 (ja) 2016-06-22
CN101421396A (zh) 2009-04-29

Similar Documents

Publication Publication Date Title
ES2720287T3 (es) Fructosil aminoácido oxidasa
ES2807530T3 (es) Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona
ES2391802T3 (es) Composición para el ensayo de proteínas glicosiladas
US9062286B2 (en) Amadoriase having altered substrate specificity
US7816116B2 (en) Modified creatinine amide hydrolase having improved affinity for substrate, and reagent composition for determination of creatinine
US8247189B2 (en) Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
CN109563534A (zh) 糖化血红蛋白的测定方法
JP2022081510A (ja) ＨｂＡ１ｃデヒドロゲナーゼ
JP6843740B2 (ja) 比活性が向上したアマドリアーゼ
KR20160068966A (ko) 당화 펩티드에 작용하는 아마도리아제를 이용한 HbA1c 측정법
Allocati et al. Evaluation of the role of two conserved active-site residues in Beta class glutathione S-transferases
US10752934B2 (en) PQQ-sGDH mutant, polynucleotide and glucose detection biosensor
Krautwurst et al. Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: revised amino acid sequence, site-directed mutagenesis, and microenvironment characteristics of cysteines 365 and 458
RU2336306C1 (ru) Генетически сконструированная зависимая от пирролохинолинхинона глюкозодегидрогеназа, содержащая инсерцию аминокислоты
CN105331590B (zh) 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
Ueki et al. Characterization of vanadium-binding sites of the vanadium-binding protein Vanabin2 by site-directed mutagenesis
JP7090547B2 (ja) 改変型ザルコシンオキシダーゼ並びにその遺伝子及び製造法
JPWO2019045052A1 (ja) 糖化ヘモグロビンオキシダーゼ改変体及び測定方法
KR20050042771A (ko) 글루코오스 탈수소효소
Allocati et al. Glutamic acid-65 is an essential residue for catalysis in Proteus mirabilis glutathione S-transferase B1-1
WO2005113761A2 (en) Novel alanine transaminase enzymes and methods of use
EP2748312A2 (en) Cholesterol oxidase
US7914985B2 (en) Alanine transaminase enzymes and methods of use
EP1677665A2 (en) Determination of ions using ion-sensitive enzymes
CA2461065A1 (en) Transductin-1 and transductin-2 and applications to hereditary deafness